| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 73,581 | A→C | 8.7% | V314G (GTA→GGA) | thiP ← | thiamine/thiamine pyrophosphate ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 73,581 | 0 | A | C | 8.7% | 254.3 / 3.0 | 76 | V314G (GTA→GGA) | thiP | thiamine/thiamine pyrophosphate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (61/7); new base C (2/6); total (63/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACT > NC_000913/73345‑73819 | gTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTAc > 2:33942/1‑238 (MQ=255) tAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGCTTTAcc > 1:1089030/1‑238 (MQ=255) aaaaGAACCCGGTAGCCAGCACACCCCCCGCCATGCCGAGGATAACCCTCCCGCTCACCTCCAGC‑CCTGTCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCa < 2:1137639/239‑1 (MQ=255) aagCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATcgac > 2:1009047/1‑239 (MQ=255) agcggctacccAGCCCAACCCCCGCAAGGCCGAGAACCCACATGCCGCCCATCACCCGCACCTT‑CCCCCCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACCCCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATcga < 1:630724/228‑1 (MQ=255) ccGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATcgacga > 2:711870/1‑238 (MQ=255) ccGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATcgacga > 2:703101/1‑238 (MQ=255) ccGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATcgacga > 2:502804/1‑238 (MQ=255) ccGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATcgacga > 2:351341/1‑238 (MQ=255) gCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTCCCGCGCCCGCAGTTCGCGACTGCTCCATCGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGCCCACAGCGCCTGCCA‑CA‑GCACCGGTACCGCCA‑GCACTTCCGGCAACTGG‑CGATCTCCCCCATCGACGACCACCGcgcg > 1:124278/1‑235 (MQ=255) tCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCCTAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGCCCACAGCGCCTGCCA‑CA‑GCCCCGGTTGCGCCC‑GCCCTCCCGGCCACCGG‑CGATTTACCCCACCGACGACCACCGCCAGTCAccgc > 1:473608/1‑236 (MQ=255) aCATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGcc > 2:1079135/1‑239 (MQ=255) ccGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGcac > 2:1066201/1‑239 (MQ=255) cGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGcaca > 2:432982/1‑239 (MQ=255) cTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCCGCGCCAGCACAAt > 2:483028/1‑239 (MQ=255) cTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAt > 2:797558/1‑239 (MQ=255) cTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAt > 2:824337/1‑239 (MQ=255) cTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAt > 1:825480/1‑239 (MQ=255) aTCTCCAGCACCTG‑ACCCCCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCCCTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCATTTCCGGCACCTGG‑CGATTTCCCCCATCGCCGATCACCGCCAGTAACGGTAGCAGCAACAGCAGCAGCGCCAGCACAATTa > 1:525865/1‑238 (MQ=255) ccTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCACCGCCAGCACAATTAACCACGTGTc > 1:751785/1‑238 (MQ=255) tG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTACCACCGTGTCGCa > 2:816556/1‑239 (MQ=255) tG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCa > 2:888836/1‑239 (MQ=255) g‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAaca > 2:487365/1‑228 (MQ=255) g‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCaa > 2:551956/1‑239 (MQ=255) g‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCAAGCACAATTAACACCGTGTCGCaa > 2:908857/1‑239 (MQ=255) ccAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCg > 2:782078/1‑238 (MQ=255) gcgcCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCGCAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAaca > 2:566695/1‑207 (MQ=255) cTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCtt > 2:163557/1‑238 (MQ=255) aaccacacggcaccacaccacACACAATCCCCCCGCCCCCAGCACAACGCGAAACGGGGTCCCCACCGCC‑GCCACCA‑CCCCGGGTTGCGCCG‑CCACTCCCGGCAACGGG‑CGATTCCCCCCACCGACAATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCg < 1:996246/224‑1 (MQ=255) gcaTCATGGTCAGCACTACGCACAATCCACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtg > 1:500483/1‑239 (MQ=255) gcaTCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAaca > 2:272629/1‑177 (MQ=255) catcatGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtgg > 2:496664/1‑239 (MQ=255) catcatGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtgg > 1:122281/1‑239 (MQ=255) catcatGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtgg > 1:1022472/1‑239 (MQ=255) atGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGGGcc > 1:1090921/1‑239 (MQ=255) ccacaCGCACAACACCCCCGCCGCCAGCGAAACACCCAGCGAGGCCCACAGCCCCGGCCA‑CC‑GCCCCGTTTGGGCCA‑GCACTTCGGGCAACGGG‑AGATTCACCCAATCGCCTATCTCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCaa < 1:908857/235‑1 (MQ=255) cACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCaa > 2:165052/1‑239 (MQ=255) cACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCGTGCAGCAGCGTGGTGCCCGGCGCaa > 1:1085684/1‑239 (MQ=255) cGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATgg > 2:1101198/1‑237 (MQ=255) cGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGACA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCCACTGG‑CGCTTGACCCCAACGACGATCACCGACAGTAACGGTGGCAGCAACAGCAGCAGCGCCACCACAATTAACACCGTGTCGCAAATGCCGCTATGCAGGCCACCGACCGGGTCGCGGCAGCCTTgcagcacacagacgccgggcgccacggc > 1:830196/1‑216 (MQ=255) aaTACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGAGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTAc > 1:1142467/1‑238 (MQ=255) acCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGGGGTGCCGGGCGCAATGGCCTTACTCAATc > 1:290027/1‑239 (MQ=255) cTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATc > 1:236317/1‑237 (MQ=255) ccgccAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTCCTCAATCGCTg > 1:919590/1‑238 (MQ=255) ccgccAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTg > 1:658758/1‑238 (MQ=255) gccAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTACCGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCCCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCCTACTCAATCGCTgcc > 1:851665/1‑236 (MQ=255) aGCGCAATACGCAACGAGGTCCACAGCGCCTGCCT‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTCTGCAGACGATCGTCCGGGTCGCCCCAGCCTTGCAGCAGCGCGGAGCCGGGCGCAATGGCCGTACTCAATCGCTGACtaa > 1:439908/1‑236 (MQ=255) aGCGCAATACGCAACGAGGTCCACAGCGCCGGACA‑CA‑GCAACGGTTGCGCCA‑GCACTTCCGGCAACTGG‑CGCTTTACCCCATCGCCGCTCACCCCAAGGAACGGTGGCTGCAACAGCAGCAGCGCCAGCACAATTATCACCGTGTCGCAAATGCGGCTAGGCAGACGATCGTCCGGGTGTCGCCGCGCTTGTACAGGCGGAGTGCGGGGAGAAATGGCATTACTCAATCGATGAccat > 1:696511/1‑235 (MQ=255) gcAACAGGCAGCGAGTGCCACAGCGCC‑GCCC‑CACGCGCCTGTTGT‑CCA‑CGCCCTCCGGCAACCGGCCCATCTCCCCCATCGGCGTACACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAACACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCaa < 1:703101/236‑1 (MQ=255) aTACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑ACACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGACAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGCCGATCGTCCGGGGCGCGCCAGCCTCGCAGCAGCGGGGGCCCGGGCGCAATGGCCTTACTCAAACGCTGACTCAACAGCa > 1:396030/1‑238 (MQ=255) tCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGgca > 2:391982/1‑238 (MQ=255) cggctcccgCCA‑CA‑GCTCCGG‑TGGGCCACCCACTCCCGGCAACGGG‑CAATTTCCCCAACCGACAACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcaca < 1:861418/231‑1 (MQ=255) cacgcgtcgCCA‑CG‑GCACTGTTTGCGCCG‑GCCGCTCCGTCATCTGA‑CTATTCACCCAACAGAGGCCCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcaca < 1:159197/231‑1 (MQ=255) ccTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCa > 1:669073/1‑237 (MQ=255) ccTGCCA‑CA‑GCACCGGGTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCCGCGTGGTGACGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCa > 2:735534/1‑237 (MQ=255) cTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCa > 1:405874/1‑236 (MQ=255) gCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTgg > 2:1021663/1‑239 (MQ=255) a‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGagca > 1:1148575/1‑238 (MQ=255) accgggtggcccggcctctCCGGCAGCGCG‑CGATTCACCCCACCGACCATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAgcgc < 1:163557/221‑1 (MQ=255) gTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGc > 1:1188233/1‑239 (MQ=255) gTTGTGCCA‑GCACTTCCGAAAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGAGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATACAGACGATCGTCCGGGTCGCGCCAGCCTTACAGCACCGAGGGGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAAAAGCCCCAGCCCGAGGCAGCACACCATCTGGAACAGCCCCAGc > 1:957998/1‑239 (MQ=255) gtgCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCAtt > 2:82614/1‑239 (MQ=255) gCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCACCAGCATTg > 1:1035797/1‑238 (MQ=255) ccA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGcc > 2:1126946/1‑239 (MQ=255) a‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcg > 1:326344/1‑238 (MQ=255) ccgcccccGGAAACTGG‑TGATTCACCCCACCGACGCACACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgcg < 1:551956/233‑1 (MQ=255) cccctcgcgacaacgcg‑CGATATCCCCCAGCGAGCATCACCGCCAGTAACGGGGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgcg < 1:782078/223‑1 (MQ=255) aCTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgcgcg > 1:596935/1‑239 (MQ=255) gccgggCAACGGG‑CGTTTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCggg < 2:756161/235‑1 (MQ=255) cTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGt < 2:326344/239‑1 (MQ=255) tGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTa > 2:677453/1‑239 (MQ=255) tGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTa > 2:58387/1‑239 (MQ=255) cggcagttttCCCCCATCGCCGTCCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGt < 2:344695/232‑1 (MQ=255) g‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGag > 2:137133/1‑204 (MQ=255) g‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAg > 1:1180060/1‑238 (MQ=255) g‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCAAGCACAATTAACACCGTGTCGCAAATGGGGTTATGCAGCCGATCGTCCGGGACGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATAGATGACTCAACAGCACCAGCCCGAGGCAGCACCCCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAg > 2:717048/1‑238 (MQ=255) aCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTTCTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAAc > 2:808426/1‑238 (MQ=255) aCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACt < 2:825480/239‑1 (MQ=255) | GTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTG‑ACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA‑CA‑GCACCGGTTGTGCCA‑GCACTTCCGGCAACTGG‑CGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACT > NC_000913/73345‑73819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |