Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,383,710 C→A 26.2% L84F (TTG→TTT)  ycjW ← LacI family putative transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,383,7100CA26.2% 234.7 / 27.9 108L84F (TTG→TTT) ycjWLacI family putative transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (59/19);  new base A (2/27);  total (62/46)
Fisher's exact test for biased strand distribution p-value = 6.35e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

GACAGAATAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATAAA  >  NC_000913/1383473‑1383948
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
gACAGAATAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCa                                                                                                                                                                                                                                                  <  2:172434/239‑1 (MQ=255)
     aaTAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACt                                                                                                                                                                                                                                             >  2:231105/1‑239 (MQ=255)
      aTAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCaa                                                                                                                                                                                                                                                 <  2:221395/234‑1 (MQ=255)
      aTAAACATGGGCATATTGACCTTCAACGTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCaaaa                                                                                                                                                                                                                                               <  2:686034/236‑1 (MQ=255)
          aCATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑aga                                                                                                                                                                                                                                       >  2:865085/1‑239 (MQ=255)
               ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAACTGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGAGGTGATGGCATGCAAAACTTC‑AGAGaaaa                                                                                                                                                                                                                                  >  2:454530/1‑239 (MQ=255)
               ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGaaaa                                                                                                                                                                                                                                  >  2:1042969/1‑239 (MQ=255)
               ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGaaaa                                                                                                                                                                                                                                  >  2:80858/1‑239 (MQ=255)
               ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGaaaa                                                                                                                                                                                                                                  >  2:571408/1‑239 (MQ=255)
               ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGACTTTAATCATTTTATGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGAGGTGATGGCATTCAAATATTC‑AGAGaaaa                                                                                                                                                                                                                                  >  1:897315/1‑239 (MQ=255)
                    atTGACCTTCCACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGCTGGCATGAAAAACTTC‑AGAAAAAAA‑Cgta                                                                                                                                                                                                                            >  1:1160659/1‑237 (MQ=255)
                     ttGACCTTCAACTTTGCCAATCACCACCACCGGCATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGAt                                                                                                                                                                                                                           >  2:313411/1‑239 (MQ=255)
                        aCCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTa                                                                                                                                                                                                                         >  1:131709/1‑238 (MQ=255)
                         aattgtgtaaaagaaacagcccccacgGGAATATAATATTTACCGATTTGGGCAAAAAATTACTCATCCGTTGGCGAACTGAGC‑ATAATAATGCCTTTATTCATTTTCTGTTTAATTTTGCTTC‑CGCTTTTTTGCAGATCTTCTGCCGGATTGGGG‑GCCGTTTG‑TAATTTCCCGTCGAAACC‑TC‑TTCCTT‑CA‑GCTTTGGCGGTGTTGGCATGCAAAGCTTA‑AGAAAAAAA‑CGTATTccc                                                                                                                                                                                                                       <  1:1084510/217‑1 (MQ=255)
                            tCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACccg                                                                                                                                                                                                                     >  2:137633/1‑238 (MQ=255)
                            gtaagatgggtaaaagaggcggccGGAATATCATTTTTATCGAGTGGGAAAAAAAAGGACTCACCCGCTGGCGAATTGAGC‑ATAATAATGCTTTTAATCTTTTTCGGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGTTTGTGC‑GACGTTTG‑TAATACCCCTTCAAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCA‑GCAAAACCTCAAGAGAAAAA‑CGGATTccc                                                                                                                                                                                                                       <  1:1169804/214‑1 (MQ=255)
                             cAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGTAAAACTTC‑AGAGAAAAA‑CGGATTACccgcc                                                                                                                                                                                                                   >  2:93525/1‑239 (MQ=255)
                             cAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACccgcc                                                                                                                                                                                                                   >  2:1130212/1‑239 (MQ=255)
                             aaaCTTTGCCAACCACCACCCCCTAAATGTAATGTTTTAAAAATGGGAAAAAAAATGACTCATCCGTTGGCAAAC‑GAGCAATAATAATGCCTTTAAATATTTTCTGCTTAATTTTGCTTT‑CGCATTTTGGAAAATCTTCTGCGGGATTTTGC‑GGGGTTTG‑AAAAACCCCGCCAAACCC‑TT‑CTTCTT‑CG‑GCTTTGGCGGTGATGGCATGAAAAATTTC‑AGAAAAAAA‑CGGATTACccgcc                                                                                                                                                                                                                   <  1:934849/238‑1 (MQ=255)
                              aaCTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACccgccg                                                                                                                                                                                                                  >  2:982344/1‑239 (MQ=255)
                              aaCTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGCGCAAAAAATGACTCATCCGCTGGGGAACTGCGC‑ATAATAATGCCTATAATCATTTTCTGCTTAATTTTGCTTT‑CGCCTTTTTGCAGATCTTCTGCCGGATTGTGC‑GCCGTTTG‑TAATATCACGTCGACACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGCATTGCAAAATTTC‑CGATATAAC‑CGGATTACccgtcg                                                                                                                                                                                                                  >  1:1018344/1‑239 (MQ=255)
                                                  ccggataaactaatTTATGGAGTTGGAAAAAAAAGGTCTCATCCTCTGTGACAGTGAGA‑TTAATAATGCTTTTATCCTTTTTCTTCTTTATTTGGTTTT‑CGCATTTTTCCATATTTTCTGCCGTATTGGGC‑GAGGTTTG‑AAATACCACGTCAAACCC‑TT‑CTTCTT‑CA‑TCTTTGGCGGTTATGGAATGAAAAATTTC‑AGAGAAAAC‑CGGATTGCCCGCCGTAGTTTTGGTCGAACGGGTa                                                                                                                                                                                              <  1:690871/227‑1 (MQ=255)
                                                    taaataatatatTTAGCGAGTTGGGAAAAAAATGCTTCTCCCGCTGGCTAAATGAAC‑ATAAAAATTCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTGGCAGTGCTTCGGCGGGTTTGTGC‑GCGGTTTG‑AAATACAACGGCGAAACC‑TT‑CTTCTC‑CA‑GCTTGGGCGGTGGTGGCATGAAAAGCAAA‑AGAGAAAAC‑CGTATTACCCGCCGTAGTTTTGGTCGAACGGGTAGa                                                                                                                                                                                            <  1:440180/231‑1 (MQ=255)
                                                     gAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGaa                                                                                                                                                                                           >  2:935662/1‑239 (MQ=255)
                                                       attaaatttttATCGAGTGGGAAAAAAAAGGACAACTCCGCAGGCGAAAGGAGC‑ATAATAATGCTTTAAATCTTTTTCTGCTTATTTTTGTTTT‑CGTATTTTTGCAGCTCTTCTGCTGTATTGTGC‑GACTTTTT‑TAATGTCACGGAAAACCT‑TT‑CTCCTT‑CA‑CTGTTGGCGGTGTTGGAATACAAAACTAC‑AGAAAAAAG‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAAt                                                                                                                                                                                         <  1:490461/231‑1 (MQ=255)
                                                       atatCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGtt                                                                                                                                                                                                             >  2:1084510/1‑219 (MQ=255)
                                                          tCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTCGTTTTGGCCGAACGGGTAGAAATCa                                                                                                                                                                                       >  2:690871/1‑238 (MQ=255)
                                                           cATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAcc                                                                                                                                                                                     >  2:368976/1‑239 (MQ=255)
                                                             tatTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGtt                                                                                                                                                                                                             >  2:1169804/1‑213 (MQ=255)
                                                             tatTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCaacct                                                                                                                                                                                   >  1:252816/1‑235 (MQ=255)
                                                             tatTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCAt                                                                                                                                                                                   >  1:75213/1‑239 (MQ=255)
                                                                ttatgtaGTGGGGCAAAAAAAACCTTATCCGCGGGCTATCTGACA‑GAAATATTGCTTTTATTCATTTTTTTTTTATTTTTTTTTG‑TGCTTTTTTCAAGTTTTTCTG‑CGGGTTGGGG‑GGGTTTTGATAAAACCATGTC‑AAAAC‑TT‑CCTCTT‑TACGCTTTGGGGGTGGTGACATACAAAACTTA‑AGAGAAAAA‑CGAATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAAt                                                                                                                                                                                <  1:258912/233‑1 (MQ=255)
                                                                  aTCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGCGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGAGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGTGGACAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATg                                                                                                                                                                               >  2:744016/1‑238 (MQ=255)
                                                                  aTCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATg                                                                                                                                                                               >  2:516470/1‑238 (MQ=255)
                                                                      aGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATc                                                                                                                                                                          >  2:1164595/1‑239 (MQ=255)
                                                                        ttGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaa                                                                                                                                                                        >  2:338217/1‑239 (MQ=255)
                                                                             cAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAccc                                                                                                                                                                   >  2:614349/1‑239 (MQ=255)
                                                                                                   gcaaactggataaaatagtGCTTTTAATCTTTTTCTGCTAAATTTGTCTTT‑CGCATTTTTCCAGATCTTCTGCGGGATTGTGC‑GCCGTTTT‑AAATACCACGTCAAAACT‑TT‑CTTCTT‑CA‑GTTTTGGCGGTGATGGCATGAAAACCTTC‑AGAAAAAAA‑CGAATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAAt                                                                                                                                                                                         <  1:960762/183‑1 (MQ=255)
                                                                                                     accTGAGA‑ATAATAATTCTTTTAATCTTTTTCTGTTTAATGTTTCTTC‑CGCTTTTTTGCAGTTCTTCTGCCGGATTGGGG‑GACTTTTT‑TAATACACCGTCAAACCC‑TT‑CTTCTG‑CA‑GCTTGGGGGGGGAGGGCATGAAAACCTTA‑AGAAAAAAA‑CGTATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGc                                                                                                                                           <  1:1023200/237‑1 (MQ=255)
                                                                                                         gacc‑ATAAAAATCCCTTTAATTTTTTTCTGTTTAATTTTTCTTC‑CGCTTTTTGGCAGATCTGTTCCGGGATTGTGG‑GACTTTTG‑TAATATCCCGTCAAAACC‑TT‑‑TTCTTCCA‑GTTTTGGCGGGGATGGCATGAAAAACGAC‑AGAAAAAAC‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGt                                                                                                                                       <  1:324578/236‑1 (MQ=255)
                                                                                                            c‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGCTGGCCTGCACAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGCTCGAACGGGCAGAAATCACCATAATGGCATCAAAACCCGAAGCGGTAAGCGCGCGCGCAAGCTTGTTTg                                                                                                                                    >  1:312546/1‑239 (MQ=255)
                                                                                                            c‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATg                                                                                                                                                                               >  2:960762/1‑196 (MQ=255)
                                                                                                               taataaGTCTTTTATTCATTTTTTGCTTAATTTGGCTTC‑CGCTTTTTTGCAGATCTCCGGCCGGATTGTGC‑GACGTTTG‑TAATAGCACTCCGAAACC‑TT‑TTTTTT‑AA‑GCTTTGGCGGTGATGGAATACAAAACTCC‑AGAAAAAAA‑CGTATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGc                                                                                                                                  <  1:488659/239‑1 (MQ=255)
                                                                                                               taaaaaTGCCTTTAATTTTTTTCTGTTTAATGTTTCTTT‑CACTTTTTTGCAGCTCTTCTGCGGGATTGTGG‑GACGTTTG‑AAATATCCCTTCAAAACC‑TT‑CTTCTA‑GA‑GCTTGGGCGGGGATGGCATGAAAAACTGC‑AGAAAAAAA‑CGGTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGc                                                                                                                                  <  1:935662/239‑1 (MQ=255)
                                                                                                                  taatGCTTTTAATCTTTTTCTGTTTATTTTTGCTTT‑CGCTTTTTTGCAGTTTTTCTGCCGGTTTGTGC‑GCCGTTTG‑TAATACCACGGCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTAATGGAATGAAAAACTTC‑AGAAAAAAA‑CGAATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGa                                                                                                                               <  1:516470/239‑1 (MQ=255)
                                                                                                                    aggcttttAATCATTTTCTGTTTAATTTTGCTTT‑CGCTTTTTTGAAGATCTTCTGCCGGTTTGTGC‑GACGTTTG‑TAATATCAC‑TTGAAACCTTT‑CTTCTT‑CA‑GCTTGGGGGGTGATGGCAAGAAAAACTTC‑AGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATa                                                                                                                             <  2:252816/234‑1 (MQ=255)
                                                                                                                     tGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGAt                                                                                                                              >  2:64834/1‑237 (MQ=255)
                                                                                                                       ccTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGCTGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGAt                                                                                                                              >  2:78639/1‑235 (MQ=255)
                                                                                                                       ccTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGAt                                                                                                                              >  2:1105710/1‑235 (MQ=255)
                                                                                                                       ccTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGAt                                                                                                                              >  2:18544/1‑235 (MQ=255)
                                                                                                                          tttaactgtttCTTGTTTATTTTTCTTTCCCCTTTTTTGCAGTTCTTCGGCCGGTTGGGGC‑GATTTTTA‑AAATACAACGCCAAACCC‑TT‑CTTCTC‑CA‑GTTTGGGGGGGGAGGTCATGAAAAACAAC‑AGAAAAAAC‑GGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTa                                                                                                                       <  1:571408/231‑1 (MQ=255)
                                                                                                                          ttAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTaa                                                                                                                      >  1:113876/1‑239 (MQ=255)
                                                                                                                            gataaTTTTTTGTTTAATTTTGCTTT‑CGTGTTTTTGCAGACCTTTTGCCGGTTTGGGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑TCTTGGGCGGGGATGGAATGAAAACCTGA‑AGAGAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAAtt                                                                                                                    <  1:263935/235‑1 (MQ=255)
                                                                                                                                  tttCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCaa                                                                                                              >  2:569470/1‑239 (MQ=255)
                                                                                                                                   ttCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAAt                                                                                                             >  2:659262/1‑239 (MQ=255)
                                                                                                                                       gCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGc                                                                                                          >  2:832903/1‑238 (MQ=255)
                                                                                                                                        cTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATggtca                                                                                                        >  2:879327/1‑236 (MQ=255)
                                                                                                                                                         cttttttGCAGATTTTCTGTGGGTTTTGGC‑GTCTTTTT‑AAAAATCACTGCCAACCC‑TT‑TTCTTT‑CA‑GTTTGTGCGGTG‑TGGGACTCAAAACTTT‑CGAAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTcgcg                                                                                        <  1:614349/237‑1 (MQ=255)
                                                                                                                                                           tttttGAAGATTTTTGGCGGGATTGTGC‑GACGTTGT‑TAATACCAGGTCAAAACC‑TT‑TTTCTC‑CA‑GCTTGGGGGGTGATGGAATGAAAACCTTC‑AGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTcgcgcg                                                                                      <  1:1105710/239‑1 (MQ=255)
                                                                                                                                                           tttgtGCAGATCGTGTGCCGGATTGTGG‑GACGTGTG‑TAATAGCCCGTCGAAACT‑TT‑TTTCAT‑AC‑GTTTGGGGGGGGACGGGATGAAATACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTcgcgcg                                                                                      <  1:88346/239‑1 (MQ=255)
                                                                                                                                                                   gattgtctGCCGGTTTGGGC‑GACGTTTT‑AAATATCACGTCGAACCC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCAGGCAAAACTTC‑AAAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCgg                                                                               <  1:865085/233‑1 (MQ=255)
                                                                                                                                                                    tttttctGCCGG‑TTGTGCGGACTTTTG‑TAAAAACACGGCAAAACT‑TC‑CTTCTT‑CA‑GCTTTGGCGGTGTTGCCAGACAAAACTTG‑AGAGAAAAA‑CGTATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCgggg                                                                             <  1:1164595/236‑1 (MQ=255)
                                                                                                                                                                    ttcttcgGCCGGTTTGTGC‑GACGTTTG‑TAATATCCCGCCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTCA‑AGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCgggg                                                                             <  2:131709/238‑1 (MQ=255)
                                                                                                                                                                      cttctGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGgag                                                                           >  2:284513/1‑239 (MQ=255)
                                                                                                                                                                           tcagatgtgtaaaagaaacgggaaaTACCACGTAGAAACC‑TT‑CTTCTT‑CA‑GTTTGGGGGGTGATGGCATGCAAACCTTC‑AAAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGat                                                                         <  1:506034/215‑1 (MQ=255)
                                                                                                                                                                             cGGTTTGTCG‑GACTTTTG‑AAATATCGCGCGGAACCC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGAGGGAATCCAAACCTTC‑AAAAAAAAC‑CGGTTTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAg                                                                    <  2:23657/239‑1 (MQ=255)
                                                                                                                                                                                aTTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGttt                                                                 >  2:1137734/1‑239 (MQ=255)
                                                                                                                                                                                       ggagtttag‑TAATATCACGACAAAACC‑TT‑CTTCTC‑CA‑GCTTTGGGGGTGATGGAATGAAAAACTTC‑AGAGAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTa                                                            <  2:1160659/230‑1 (MQ=255)
                                                                                                                                                                                       ggACGTTTG‑TAATATCACGAAGCAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATTGCATGCAAAACCTC‑AGACAAAAC‑CGGATTACCCACCGCGGTTTTGGAGGAAAGGGCAGAAATAAACATAATAGAAAAACAACCCGAAAAGGACAGCGCGCGGGCCAAGTTGTTTGGATGGAACGCGTAA‑TCCTCACTGGCCCGAAACACTTTTTCGACCCCTTCCCGGGAAGTTATAGCTTGCTTa                                                            >  1:579566/2‑238 (MQ=255)
                                                                                                                                                                                            tttG‑TAAAATCCCGTCGAAACC‑TT‑CTTCTC‑AG‑GCTTTGGCGGTGATGGCATGAAAAACTTC‑AGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGc                                                      <  2:1096785/239‑1 (MQ=255)
                                                                                                                                                                                            tttG‑AAATATCCCGACGAAACC‑TT‑TTTCTT‑CA‑GCTTGGGGGGTGAGGGCATGAAAAGCTTC‑AGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGc                                                      <  1:64834/239‑1 (MQ=255)
                                                                                                                                                                                              ttttaaataccccgacaaaCC‑TTGCTTTTT‑CA‑GCTTGGGCGGTGATGGCATGAAAAGCTCA‑AGAAAAAAA‑CGGATCCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGc                                                      <  1:982344/223‑1 (MQ=255)
                                                                                                                                                                                              tG‑TAAAACCACGTCAAAACC‑TT‑CTTCTT‑CA‑GTTTTGGCGGTGATGGCAGGAAAAACTAC‑AAAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAc                                                                                                                            <  1:375406/168‑1 (MQ=255)
                                                                                                                                                                                               g‑TAATATCCCGTCAAACCC‑TT‑CTTCTT‑CG‑GTTTTGGGGGTGATGGCAGAAAAACCCTC‑AGAAAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCAcg                                                   <  2:1077560/239‑1 (MQ=255)
                                                                                                                                                                                                  aaTATCACGTAAAATCC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGAGGAAATGAAAAGCTAC‑AAAAAAAAA‑CGGTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCAcgcg                                                 <  2:130434/239‑1 (MQ=255)
                                                                                                                                                                                                       cACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTTGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCAAGTTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGGAACACTTTTTCGCGCGCCTCCGGGGCGATCTTAGTTTGCTTATTCAGCACGGGTgct                                             >  1:198310/1‑236 (MQ=255)
                                                                                                                                                                                                       cACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCtt                                                                                                                                         >  2:475239/1‑147 (MQ=255)
                                                                                                                                                                                                       aaCGGCGAAACC‑TT‑CTTCGT‑CA‑GCTTTGGCGGCGATGGAATAAAAAACTTG‑AGAGCAAAA‑CGGATTACCCGCTGTAGTTTTGGTCGAACGGGTAAAAACCACCATAATTGAATCAAAACCCGAAGGGTTAAGCGAGTTGGGCAGCATGTTTGGCTGATAC‑TGTAATTCTTCAATGGCACGTAACACTTTTTCGCGGCTTTCCCGCACGATATTAGTTTGCTTATTCAACCcgcggaata                                            >  1:866193/2‑234 (MQ=255)
                                                                                                                                                                                                        aCGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGGAGTTTTGGTCGAACGGGGAGAAATCACCATAATGGCATCAAAACCAGAAGAGGGCAGCGCGCGGTCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATAc                                           >  2:400547/1‑239 (MQ=255)
                                                                                                                                                                                                         cGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTa                                                                                                                       >  2:902169/1‑162 (MQ=255)
                                                                                                                                                                                                         cGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAAttctt                                                                                                                 >  2:375406/1‑168 (MQ=255)
                                                                                                                                                                                                           tCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGc                                                               >  2:506034/1‑216 (MQ=255)
                                                                                                                                                                                                           tCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGt                                        >  2:457740/1‑239 (MQ=255)
                                                                                                                                                                                                            cGAAACC‑TT‑CTTCTT‑CA‑GTTGTGGCGGTGATGCCATGAAAAACTTA‑AGAGAAAAC‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGt                                        <  1:711188/238‑1 (MQ=255)
                                                                                                                                                                                                                cgaaatcttttccttagtttttGGCGGTAATGGAATCCAAACCTCA‑AAAAAAAAA‑CTGATTGCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGt                                        <  1:998710/219‑1 (MQ=255)
                                                                                                                                                                                                                     t‑cttctt‑cA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGa                               >  2:603684/1‑239 (MQ=255)
                                                                                                                                                                                                                              cA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACg                           >  2:1140903/1‑236 (MQ=255)
                                                                                                                                                                                                                               a‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAAtt                                                                                                                    >  1:594545/1‑146 (MQ=255)
                                                                                                                                                                                                                               a‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAAtt                                                                                                                    <  2:594545/146‑1 (MQ=255)
                                                                                                                                                                                                                                tgcttgtgCGGTGAGGGCATGCAAAACTAC‑AGAAAAACA‑CGATTTCCCCGCCTTAGTTTTGGTCGAACGGGTAGAAATCACCATTATGGCATCAAAACCCGAAGAGGTCAGCGCTCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTg                       <  2:257843/232‑1 (MQ=255)
                                                                                                                                                                                                                                  cTTTGGCGGTGATGGCATGCAAAACTTA‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGTAGAGGCCAGCGCGCGGGCCAGCTTGTTTGGCAGATAC‑TGTACTTGTTCAATGGACCGTAACTCTTTTTTGCGCGCTTCCGGGGAGATTTTAGTTTGCTCATTCAGCACGCGTGATAAGGTGGTGTTTGATACGCCTgaa                     >  1:257843/1‑237 (MQ=255)
                                                                                                                                                                                                                                            gagggaaTGAAAAACTAC‑AAAGAAAAC‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa            <  1:284513/232‑1 (MQ=255)
                                                                                                                                                                                                                                            gATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa            <  2:113876/238‑1 (MQ=255)
                                                                                                                                                                                                                                            gATGACACGAAAAACTTG‑AGAAAAAAA‑CGAATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa            <  1:18544/238‑1 (MQ=255)
                                                                                                                                                                                                                                            aagggCATGCAAAACGGA‑AAAAAAAAA‑CGGATAGCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTGTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa            <  1:338217/235‑1 (MQ=255)
                                                                                                                                                                                                                                               ggaaTGAAAACCTTC‑AAAAAAAAA‑CAGTTCCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAata         <  1:832903/235‑1 (MQ=255)
                                                                                                                                                                                                                                                 cATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGGGGGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCa      >  2:49779/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 cATGAAAATCTGC‑AGAAAAAAA‑CAGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATc       <  1:1137734/238‑1 (MQ=255)
                                                                                                                                                                                                                                                  aTGAAAAACGTA‑AAAAAAAAA‑CGAATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCAt     <  1:988110/239‑1 (MQ=255)
                                                                                                                                                                                                                                                     cAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATaaa  >  2:549789/1‑239 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
GACAGAATAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGC‑ATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTT‑CGCATTTTTGCAGATCTTCTGCCGGATTGTGC‑GACGTTTG‑TAATATCACGTCGAAACC‑TT‑CTTCTT‑CA‑GCTTTGGCGGTGATGGCATGCAAAACTTC‑AGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAC‑TGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATAAA  >  NC_000913/1383473‑1383948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑