Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,576,077 C→A 14.7% D515Y (GAT→TAT)  yddB ← putative TonB‑dependent outer membrane receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,576,0770CA14.7% 186.9 / 3.0 62D515Y (GAT→TAT) yddBputative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base C (45/6);  new base A (2/8);  total (48/14)
Fisher's exact test for biased strand distribution p-value = 3.71e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTAT  >  NC_000913/1575847‑1576313
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGATTGCTTTTGATAATTTGATCATGCGATTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGTTTTTCTGCTGCTGTCCCATTGCCAGTTCATCGTTATCAGGCGTTTTCAAATCC‑TG‑ATCACGCGTCAGAGTTTTCTTACCTGTTACCGATTCCGT‑CCAGCTATTACGGATATCACGTTATCCCATTTCacgcatct                                                                                                                                                                                                                                      >  1:372496/1‑232 (MQ=255)
aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAatat                                                                                                                                                                                                                                      >  2:110312/1‑239 (MQ=255)
aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAatat                                                                                                                                                                                                                                      >  2:55195/1‑239 (MQ=255)
aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAatat                                                                                                                                                                                                                                      >  1:563173/1‑239 (MQ=255)
aGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CAAGCTATTGCGGATCTCACGTAATCCCATATCAAGAatat                                                                                                                                                                                                                                      >  1:1065844/1‑239 (MQ=255)
  tAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATAtt                                                                                                                                                                                                                                     >  1:592613/1‑238 (MQ=255)
  tAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATAtt                                                                                                                                                                                                                                     >  2:124897/1‑238 (MQ=255)
  tAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATAAAGAATAtt                                                                                                                                                                                                                                     >  2:949385/1‑238 (MQ=255)
     tggtggTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTccc                                                                                                                                                                                                                                  >  2:553358/1‑238 (MQ=255)
     tggtggTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTccc                                                                                                                                                                                                                                  >  2:337557/1‑238 (MQ=255)
       gtggtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGcc                                                                                                                                                                                                                               >  2:651016/1‑239 (MQ=255)
          gtTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATa                                                                                                                                                                                                                            >  2:1090785/1‑239 (MQ=255)
           ttttAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAg                                                                                                                                                                                                                           >  2:407447/1‑239 (MQ=255)
            tttAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCTGCCATAGt                                                                                                                                                                                                                          >  2:846968/1‑239 (MQ=255)
                    cgcTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTta                                                                                                                                                                                                                  >  2:273506/1‑239 (MQ=255)
                        gTCGGTACGATTTCTTTTGCTGTTTTGATCATGCGCTTCACGGTAAAAATAGTTTGCACGCGCAATAACTTTCTTACCGATTTTCTGCTGCAATCCCATTGCCATTTCATCTTTATAAGGCTTTTTAAAATCC‑TG‑ATAACGCGTCAGATTTTTTTTACCTAATACCTATCCCGT‑CCAGCTATTGCGGATATAACTAAACCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAAc                                                                                                                                                                                                              <  2:563173/239‑1 (MQ=255)
                                   tGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATc                                                                                                                                                                                                   >  2:216931/1‑239 (MQ=255)
                                   tGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCACATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAAta                                                                                                                                                                                                   >  1:1090410/1‑238 (MQ=255)
                                                    tCATGCGCTTCACGTTAAACATAGTTTGCACGCGAAATAACGTTCTTACCGATTTCTTGCTGCAACCCCATTGCCTTTTCATCTTTAAAAGGCTTTTTAAAATCC‑TG‑ATACCGCGTCATATTTTTATTACCTAATACCGATTCCGT‑CCAGCTATTGCGAATATCACGTACCCCAATATAAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAg                                                                                                                                                                                   <  2:592613/238‑1 (MQ=255)
                                                        gcgcTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaa                                                                                                                                                                              >  2:372333/1‑239 (MQ=255)
                                                          gcTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAat                                                                                                                                                                            >  2:1078909/1‑239 (MQ=255)
                                                                     aaCATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCg                                                                                                                                                                 >  2:449967/1‑239 (MQ=255)
                                                                        aTAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCAGATGCAATCCCATTGCCAGTTCACCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCACAGTTTTATTACCAGATACCGATTCCGT‑CCAGCTATTGCGGATATCAAGTACTCCCATATTAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATAACTGTAGCTTGATTATCAACAATATCCCAATCCgc                                                                                                                                                                >  2:993454/1‑236 (MQ=255)
                                                                             ttGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATaaa                                                                                                                                                          >  2:1190509/1‑238 (MQ=255)
                                                                             ttGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTCATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATaaa                                                                                                                                                          >  1:1159125/1‑238 (MQ=255)
                                                                             aatagcaagcAATACCTTTCTCACCTATTTTCTTCTGCACCCCCATTGCCACTTCTTCTTTATAAGGC‑TTTTTAAATCCTTG‑AAAGCGTTACAGTTTTTTTTTACCTAACCCCTATCTGCC‑CCAGCTATGGCGGATTTATCTAAACCCTATATCAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAg                                                                                                                                                         <  1:831976/231‑1 (MQ=255)
                                                                                     gaaaTAACTTTCTTACGGATTTTCTCCTCCACCCCAATTGCCA‑TTCAATGTTAAAAAGGCTTTTCAAAATC‑TGAAAAACGCGTCAGATTTTTATTCCCGGATACCGATCCCGC‑CCACTTTTTGCGAATACCACTTAACCCAATACAAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCgggg                                                                                                                                                  <  2:12770/236‑1 (MQ=255)
                                                                                            tttttgtACCGTTTTTCCGCTCAACTCCCATGGCCATTTATTCGTTATAGGTCTTTTACAAACCC‑AT‑ATAACGCGTAAGATTTTTATTCCTTGATCCCGTTTCCGC‑CCAGCTTATGGGAATATCACGTAACCCAATATCAAAAATTTCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATtg                                                                                                                                          <  1:337557/233‑1 (MQ=255)
                                                                                            ctttcttcCCGTTTTTCTGCTACAACCCCCTTCCATTTTCATGTTTATAGGTCGTTTTCAAATCT‑TT‑ATACCGCGTCATTTTTTTATTCCTTGATACCGACTCTGT‑CCAGTTATTGCGATTATCACATAATCCCATATAAAGATTTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATtg                                                                                                                                          <  1:449967/237‑1 (MQ=255)
                                                                                                cTTGCCGATTTTCTGCGCCAAGCGCATGGCCAGTTATTTGTTAGAGGTCGTGTTGAAATGT‑TT‑AAAGTGCGTCATATTTTATTTACCTGAT‑CGGGTTCCATCCCAGTTATTGGAGCTACAAGGAAATCCCAAATAAAAATTTTGCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGt                                                                                                                                      <  1:369642/239‑1 (MQ=255)
                                                                                                 ttACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAACGCGCGGGGAGATATTGTGGtt                                                                                                                                     >  2:169729/1‑239 (MQ=255)
                                                                                                 ttACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCTCGGGGAGATATTGTGGtt                                                                                                                                     >  2:786878/1‑239 (MQ=255)
                                                                                                 ttACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGgaga                                                                                                                                               >  2:831976/1‑229 (MQ=255)
                                                                                                 ttACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATAATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGTTATTGTGGtt                                                                                                                                     >  2:159314/1‑239 (MQ=255)
                                                                                                   aCCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTg                                                                                                                                   >  2:1111707/1‑239 (MQ=255)
                                                                                                   aCCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTg                                                                                                                                   >  2:311156/1‑239 (MQ=255)
                                                                                                   aCCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGATTTCATATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATAAAGAATATTCCCGACATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTAGATTAGCAAAAATAGCACTTTACTACATAAATCGTGGGGAGAGTTTGTGTTTTg                                                                                                                                   >  1:255576/1‑239 (MQ=255)
                                                                                                           tCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAg                                                                                                                           >  2:923805/1‑239 (MQ=255)
                                                                                                               ctgcAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTg                                                                                                                        >  2:1071677/1‑238 (MQ=255)
                                                                                                                                 ttCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACg                                                                                                      >  2:902772/1‑238 (MQ=255)
                                                                                                                                  tCATCGTTAAAAGGTGTTTCAAAATCC‑TA‑AAAACCGGTCAGTTTTTTATTCCCTAATCCCGATTCCGC‑CCAGTTTTGGGAGATATCACAAAACCAAATAAAAAAATTATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGcc                                                                                                    <  1:110312/239‑1 (MQ=255)
                                                                                                                                           taAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTaa                                                                                           >  2:617061/1‑239 (MQ=255)
                                                                                                                                              ggCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGATATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGCTGTCATAGCCATACCGCCCGCCGCGCATTAATg                                                                                         >  1:105307/1‑238 (MQ=255)
                                                                                                                                                cGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGac                                                                                       >  2:863532/1‑238 (MQ=255)
                                                                                                                                                  ttttCAAATCC‑TG‑ATAACGAGTCAGAGTTTTATTACGTGACACCGATACCGT‑CCAGCTATTGAGCATATCACGTACTCCCATATCAACAATATTCAGACGATAGTAACGGTTATAACTCGCTGTACTCACAGCAGCTTGATTAGCAAACATATCCAATTCCGTACTATAGAGCGGGCCGTTATCGTGGTTTGCCAGATCAGTATCATAGTCATACCGCACGCCGGGCATTACTggaaca                                                                                    >  1:223426/1‑234 (MQ=255)
                                                                                                                                                         aataccgaaTAACGGGTCATTTTTTTTTACCCTAATACCGCTTCCTA‑CCAGCTATTGCGAATAACACAAACCCCAATATAAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACg                                                                               <  1:273506/231‑1 (MQ=255)
                                                                                                                                                                     cgcgTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATaaa                                                                                                                                                          >  2:475058/1‑152 (MQ=255)
                                                                                                                                                                        gTCAGATTTTATTTGCCTAATACAAATTCCCT‑ACATTTATTGCTAATATAACGTACCCCAAAATAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGAt                                                                 <  2:372496/238‑1 (MQ=255)
                                                                                                                                                                              tttttatcccgcgatcctcaTTCCGT‑CCAGTTATGGCGTATATCATTTCACCCTATACCATGACTCTCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCat                                                          <  1:786878/220‑1 (MQ=255)
                                                                                                                                                                                           aTACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAg                                             >  2:945255/1‑239 (MQ=255)
                                                                                                                                                                                           aTACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAg                                             >  2:425660/1‑239 (MQ=255)
                                                                                                                                                                                                 aTTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATaa                                                                                                                                                                                                               >  2:839383/1‑71 (MQ=255)
                                                                                                                                                                                                  ttCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGGAACGGTTATAACCTGCTGTAATCATTGCGGTTTGACTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGAGGTTTGACAGATAGTTGTCATAGGCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGAGATCAGCCATATACGGCGTGTAGTTGCCaa                                      >  2:23643/1‑239 (MQ=255)
                                                                                                                                                                                                      gT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTc                                   >  2:721783/1‑238 (MQ=255)
                                                                                                                                                                                                       t‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTccc                                 >  2:520554/1‑239 (MQ=255)
                                                                                                                                                                                                           acttatgtggaatatCACGTACTCATAAAAAAAAAATATCACCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAgg                              <  1:520554/228‑1 (MQ=255)
                                                                                                                                                                                                             cTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAggcg                            >  2:522880/1‑239 (MQ=255)
                                                                                                                                                                                                                  gCGGATATCCCGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGGTATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCCGGCGGcatt                       >  1:371610/1‑239 (MQ=255)
                                                                                                                                                                                                                      atatCACAAAACCCCATAACAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGC‑AAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacct                  <  1:171429/239‑1 (MQ=255)
                                                                                                                                                                                                                          cACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGC‑AAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTAt              >  2:171429/1‑239 (MQ=255)
                                                                                                                                                                                                                                acagtaatacAATAATCTTGCCGCCAGAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTaa         <  1:617061/230‑1 (MQ=255)
                                                                                                                                                                                                                                aTCCCACAACAAAAATATTCCCGCCATGGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGTTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGAGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTaa         <  1:1078909/239‑1 (MQ=255)
                                                                                                                                                                                                                                      tatCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggta   >  1:1003460/1‑239 (MQ=255)
                                                                                                                                                                                                                                       aacAAGAATATCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtat  <  2:915139/237‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
AGTAATGGTGGTTTTAGTCGCGCTGTCGGTACGACTGCTTTTGCTGATTTGATCATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCC‑TG‑ATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTAT  >  NC_000913/1575847‑1576313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑