Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,758 G→C 8.0% E614Q (GAA→CAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,7580GC 8.0% 176.0 / 4.8 73E614Q (GAA→CAA) rsxCSoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (43/20);  new base C (2/4);  total (46/27)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTG  >  NC_000913/1708526‑1708992
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          
agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAg                                                                                                                                                                                                                                       >  2:779173/1‑238 (MQ=255)
 gCGAAAAAGGCAAGACTCCAGGCAACTAACGACGCAGAACCCCCGGCGAATCCGCGAAAAACTGCCGTCGAAGCAGCTATCGCCCGGGAAAAAGCGGGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                                                                                                                                                                                                                                      <  1:962640/238‑1 (MQ=11)
     aaCAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGTCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAt                                                                                                                                                                                                                                 <  2:249528/239‑1 (MQ=255)
     aaCAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGa                                                                                                                                                                                                                                  >  2:581998/1‑238 (MQ=255)
          gCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAGAGGCCGATCcgc                                                                                                                                                                                                                             >  2:982866/1‑238 (MQ=255)
                  gcagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCGGCTATTGCCCGCGCCAAAGCCCGCAAACCGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGGTCCACGCAAAccc                                                                                                                                                                                                                     >  1:123470/1‑238 (MQ=17)
                   cagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCCCGCAAGCTGGTCTAGCAACAGGCTAATGCGGAACCAGAATAACAGGTTGATCCGAGCAAAgctg                                                                                                                                                                                                                    >  2:683895/1‑236 (MQ=18)
                    agcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCTCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCCCGCAAAGCCGCCGCCGAAGCCGCTATTGCCCGTGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAAAGCCGAACCCGAAGAACAGGTCGAACCCCCCAAAgacgc                                                                                                                                                                                                                   >  1:838751/1‑238 (MQ=18)
                        aaCTAACGACGCAGCAACTGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGa                                                                                                                                                                                                             <  2:556202/239‑1 (MQ=255)
                        aaCTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACCGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTACCCGTGCCAAAGCGCGCAACCTGGAACAGCAACAGGATAATGCGGAACCAGAAGAACCGGTCGATCAGCGCAAACCCACCATc                                                                                                                                                                                                               >  1:97727/1‑238 (MQ=18)
                        aaCTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGAGGAACCAGACGAACAGGTCGATCCGGGCAAAgccgcgctcg                                                                                                                                                                                                              >  1:882559/1‑234 (MQ=255)
                           tAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGaa                                                                                                                                                                                                            >  2:1074639/1‑238 (MQ=255)
                           tAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                           <  2:352400/239‑1 (MQ=255)
                             acgacgCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGACAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGcg                                                                                                                                                                                                         >  1:972630/1‑238 (MQ=255)
                                   cagcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCTTAGCCGCTATTGCCCGTGCCAACGCGCGCAACCGGGAACAGCACGAGGCTACTCCGGAACCAAAAGAACAGGTCGATCCGCGCAAAGCCCCCGTCGaatacccc                                                                                                                                                                                                      >  1:1112788/1‑230 (MQ=17)
                                    agcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGAAGAACAGGTCGATCCGAGCAAAGCCGCCGTCGAAGCCGCCAtt                                                                                                                                                                                                   >  2:536080/1‑238 (MQ=255)
                                    agcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACAAGAAGAACAGGTCGATCCGCGCAAGGCACCCGTCGAATCCGCTAtt                                                                                                                                                                                                   >  2:955679/1‑238 (MQ=255)
                                                  aTCCACGTAAAACTGCCGTTGAAGCAGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCa                                                                                                                                                                                    <  2:872584/239‑1 (MQ=255)
                                                    ccACGTAAACCGGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGGGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                  <  2:1057239/239‑1 (MQ=255)
                                                    ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                  <  2:1000548/239‑1 (MQ=255)
                                                    ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                  <  2:368925/239‑1 (MQ=255)
                                                       cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGCACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCTCGCAAAGCCACCGTCCAAGCCCCGATGGCCCgccccacacggcaca                                                                                                                                                                                >  1:1185490/1‑224 (MQ=14)
                                                       cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa                                                                                                                                                                               >  2:1112377/1‑239 (MQ=255)
                                                       cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCACCGTCGAAGCCGCTATTGCCCGTGCCCAAGCACACaa                                                                                                                                                                               >  2:517474/1‑239 (MQ=255)
                                                               ggacagtgtaggacatacgccccGCGCCAAAGCGCGCAACGAGGCAAGGCAACAGGAAGATGCGAAACCAGCACACCCGGACGATCCGCTCAAAGCCGCCGTCGAAGCCGCTATTGCCCTCGCAAAAGCGCCCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAATAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCACGCTGGAAc                                                                                                                                                                       <  1:826669/219‑1 (MQ=14)
                                                                 ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCAGGCAAGCTGGAACa                                                                                                                                                                      >  2:632205/1‑238 (MQ=255)
                                                                    atGATGCAGCCAGCGCCCTCCACAAAGCGCGCAAGCGGGAACAGCACAAGTCTAATGCGGACCCAGCACACCAGGTCGATCCGCGCAAAGCGCCCGTCGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGGGAAGGCGGAACCAGAAGACCAGGTCGATCCGCGCAACTCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                  <  1:1161705/238‑1 (MQ=14)
                                                                     tGAGGCAGATCTCGACCGAGAAAACGCGCGGAAGCGGGCACAACAACAGCAAAATGCGGAACCAGAACACCAGGCCGATCCACGCAAAACCGCCGTAGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                  <  1:1051264/238‑1 (MQ=14)
                                                                      gAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCTGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACa                                                                                                                                                                >  2:31801/1‑239 (MQ=255)
                                                                                     ggcgcAAAAGCGGGAAAGCTGGAACAGCAACAGGAAAAGGCGGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGcc                                                                                                                                                 <  1:795258/238‑1 (MQ=14)
                                                                                       cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAAAAGGCTAATGCCGAGCCAg                                                                                                                                               >  2:539939/1‑239 (MQ=255)
                                                                                           aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaaca                                                                                                                                           <  2:636964/239‑1 (MQ=255)
                                                                                            aaGCGGGCAAGCTGGAACAGCACCAGGCTAATGCGGAACCAGAACAACAGGACAAGCCGCGCAAAGCCGCCGTTGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                                                                                                                                          <  2:838751/239‑1 (MQ=255)
                                                                                            aaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                                                                                                                                          <  2:277588/239‑1 (MQ=255)
                                                                                                                cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCCCGCAAGCTGGAACAGCACCAGGCTAATGCGGCACCAGAACAACAGGTTGATCCGCGCAAAGCCGCCGTCGCCGCGGCTATTGCCCGCGCACAAGCCAGCAACCGGAACCCGCAACAGGCTAAAGCGGCTGCAGAACAACAgtctgataagctcaaag                                                                                                                       >  1:661551/1‑222 (MQ=11)
                                                                                                                     ggCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAGGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCGGCTATTGCACGCGCCAAAGCCCGCAAACGCGCCCAGCAACAGGCTCATCCGGAGCCAGAAAAACAGGTCGGAACACGCAAAAccgccg                                                                                                                 >  1:469200/1‑239 (MQ=11)
                                                                                                                                aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGACCAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCt                                                                                                      >  2:618780/1‑239 (MQ=255)
                                                                                                                                aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACCGGTTGATCCGCGCAAATCCGCCGTCGAAGCCGCTATGGCCCGTCCCCAGGCACGCAAGCTGCAACCGCAACAGACTCGTACGGAACCAGAACCACCGGTCGAACACCGCCCAgccgccagagatccggc                                                                                                       >  1:314648/1‑227 (MQ=14)
                                                                                                                                aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAACCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGACGCCATTGCCCGGGCCCATGACAGCAAGCTGGACCAGCATAAGGATCTTACGGAGCAAGACAGACAGGCAGATCCGCGCaaatctgatgatgacgaggcc                                                                                                      >  1:973858/1‑221 (MQ=11)
                                                                                                                                      aacaacAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGCTCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGc                                                                                                 >  2:996717/1‑238 (MQ=255)
                                                                                                                                          acaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCCGCCGTCGACGCGGCTATTGCCCGCGCCAAAGCAAACAAAATGGACCAGCAACAGGCCAATGCCGTGCCAGAAAAAACGGCCGAACAGCGTAAAGCGGGCGGCGacatgcgtattgcccgc                                                                                             >  1:882345/1‑222 (MQ=11)
                                                                                                                                           caGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGAg                                                                                            >  2:973118/1‑238 (MQ=255)
                                                                                                                                             ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCGGCTATTGCCCgcacca                                                                                         >  2:363327/1‑234 (MQ=255)
                                                                                                                                                  aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCCGAGCCAGTAAAACAGGACGATCCCCGCAAAGCCGCCGTCGACGCCGCAATTGccagtcccgacgc                                                                                     >  1:1086334/1‑227 (MQ=14)
                                                                                                                                                               ggccgcgaaggCCGCTATTCCCCGGACAAAAGCGCGCAAACAGGCACAGCAGCAGCCAAATGCGGAACCAGAAAACCGGGACGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGaa                                                                        <  1:585093/228‑1 (MQ=14)
                                                                                                                                                                 ccgTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACa                                                                      >  2:1008283/1‑238 (MQ=255)
                                                                                                                                                                     cGACGCGGCTAGTGCCCGCGCCAAAGCGCGCAAGGTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCaa                                                                  <  2:882559/238‑1 (MQ=255)
                                                                                                                                                                                  gcgcgcgCCAAAGCGCACAAGCTGTAACAGCAACAGGATAATGAGGAACCAGAAGCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGa                                                    <  2:97727/233‑1 (MQ=255)
                                                                                                                                                                                     ggagCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTATTGCGGAAGCAGAAGAACAGGTTGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATAGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGc                                                  <  2:665487/235‑1 (MQ=255)
                                                                                                                                                                                       tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGCACAGCAACCGGCTAATGCGGAGCCag                                               >  2:670995/1‑239 (MQ=255)
                                                                                                                                                                                       tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGAAACACCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAGCCGGAACAGCAACAGGCCAATGCCGAGCCAGAACAAAAGGTCGATCCGAGCCAAGCCGACGGAGAACCCGCTATTGCACGAGGCAAAGCACGCAAACCGGAACAGCAAAACGCTAAtctgacaacag                                               >  1:163754/1‑229 (MQ=11)
                                                                                                                                                                                       cgCCAAAGCGCGCAAGCTGGGACAGAAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGGCGAAGCCGCCATCGCGCGTGCCAAAGCGCGCAAGCCGGAAAAGCAGAAGGCAAATGATTAGCCAGAAGACCAGATTGAGCCGCGCAAAGCCGCCGTACACGCCGCCAGTCCCCGCGCACAAGTACACACACAGGAACAGAAAcacgagaatacggcgacaa                                               >  1:940242/2‑221 (MQ=255)
                                                                                                                                                                                        gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCaga                                              >  2:895031/1‑239 (MQ=255)
                                                                                                                                                                                        gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCAGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCaga                                              >  2:495844/1‑239 (MQ=255)
                                                                                                                                                                                           aaggcgcgcCAGCTGGGCCGGCAGCAGGGTAATGGGGAATCAGAGCAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCGAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                           <  2:44529/236‑1 (MQ=14)
                                                                                                                                                                                           aaaGCGCGCAAGCTGGAACAGCGACAGGCTAATGCGGAACCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                           <  2:1185490/239‑1 (MQ=255)
                                                                                                                                                                                            aagcagcgaaGCCGAAACAGCAGCAGGCTAATGCGGACCCAGAACAACAGGCGGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAgaagaa                                          <  1:1153031/231‑1 (MQ=14)
                                                                                                                                                                                                ggcgaaGCCGGAACAGCCGCAGGAGAATGCGGAGGCAGAACACCAGGCTGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAgg                                      <  1:1131120/235‑1 (MQ=14)
                                                                                                                                                                                                 cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCCGTGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGAGCCCAGGCCCGCAAACCGGAACAGCAACAGGCTAATCGCGAGCCAGAAGAACacg                                      >  1:141852/1‑236 (MQ=11)
                                                                                                                                                                                                  gcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGACAGAACAACAGGTCGACCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAACCGGGAACCGCAACCGGTTAAACCTGAGACAgaagaaagggtc                                    >  1:522156/1‑233 (MQ=14)
                                                                                                                                                                                                     agatGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATCGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAt                                 <  2:1112788/236‑1 (MQ=255)
                                                                                                                                                                                                                cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGCGCCAAGGCGCGCAACGCGGAACAGCAACAGGCAAATGCGGAGCCAGAAGACCAGGTTGACCCGCGCaaa                        >  1:985774/1‑237 (MQ=14)
                                                                                                                                                                                                                cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGAGCCAAAGCGCGCAAACCGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaaac                       >  2:705564/1‑237 (MQ=255)
                                                                                                                                                                                                                cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGCGCCAGAAGAACAGCTTGATCCGCGCaaa                        >  2:1091660/1‑237 (MQ=255)
                                                                                                                                                                                                                cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGATCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCCAGCCAACGCGCGCAAACTGACACACCACCCGGCCAACGCGGAGCCCGAAGAACAGGTTCATCCGCGCaagc                       >  1:235159/1‑236 (MQ=14)
                                                                                                                                                                                                                cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCACAAGCCCGCAAGCGGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCACGCAAAGCCGCCGACGACACAGCTATTCGCCGCGCCCAACCCCGAACACCGGCACAGTAACCCGCTATAGCGCAGCCCACAGAACGGGTTAATCcgaccacca                       >  1:889597/1‑231 (MQ=9)
                                                                                                                                                                                                                  acagtatAAAGCGGAAACAGCAGAACAGGGAGATCCGCGCAGAGCAGGCGGCAGAGCCGCTAGTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGACCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCCAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCt                     <  1:952582/232‑1 (MQ=17)
                                                                                                                                                                                                                     ggCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCg                 >  2:952582/1‑239 (MQ=255)
                                                                                                                                                                                                                          aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAAc                                                                                                                                                                       <  1:249433/84‑1 (MQ=38)
                                                                                                                                                                                                                          aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCGCGCCAACGGGCCCCGCCACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa             >  2:662303/1‑238 (MQ=255)
                                                                                                                                                                                                                            ccGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGc          <  2:513551/238‑1 (MQ=255)
                                                                                                                                                                                                                                aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGCGGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCCAAGCCCGCAAACGGGAACAGCAACCGGCTAATGCCGAGCCAGCAGAACAGGTTGATCCGCCCAAAgccgcctgcgccgctgat      >  1:128723/1‑227 (MQ=11)
                                                                                                                                                                                                                                aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGCACAGCAACAGGCTAATGCCGTGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCCAGCAAACAGGACCAGCACTAGGCTAATGCGGTGCCAGAAGAACAGGCTGAGCCGCGCAAAGCTGCCGtgctagaggcg      >  1:558193/1‑229 (MQ=14)
                                                                                                                                                                                                                                     gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTTGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAACCAGGAACAGAACCAGGCCAATGCGGGGCCCGAAGACAAGGATAATCCACGCAACACCGCCGCCGAACCGCCCAttt  >  1:120485/1‑237 (MQ=14)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          
AGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTG  >  NC_000913/1708526‑1708992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: