Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,219,538 C→T 8.0% A315V (GCG→GTG)  aceK → isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,219,5380CT 8.0% 300.3 / 14.4 99A315V (GCG→GTG) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base C (50/40);  new base T (4/4);  total (55/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 103 total aligned reads displayed.

TTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCT  >  NC_000913/4219301‑4219775
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 
ttGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAgcg                                                                                                                                                                                                                                                  <  2:797905/239‑1 (MQ=255)
         gcctgACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTAtt                                                                                                                                                                                                                                         >  2:50587/1‑239 (MQ=255)
          cctgACGCCGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTc                                                                                                                                                                                                                                        >  2:1163413/1‑239 (MQ=255)
          cctgACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTc                                                                                                                                                                                                                                        >  2:1111231/1‑239 (MQ=255)
          cctgACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTc                                                                                                                                                                                                                                        >  2:712755/1‑239 (MQ=255)
                   gacCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATgg                                                                                                                                                                                                                               <  2:224172/239‑1 (MQ=255)
                    acCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGTTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATgg                                                                                                                                                                                                                               <  1:128324/238‑1 (MQ=255)
                    acCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGt                                                                                                                                                                                                                              >  1:994840/1‑239 (MQ=255)
                                aGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTAATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGtt                                                                                                                                                                                                                  >  1:1052673/1‑239 (MQ=255)
                                   aTTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTa                                                                                                                                                                                                                <  1:630463/238‑1 (MQ=255)
                                         tttGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGcc                                                                                                                                                                                                         <  1:309888/239‑1 (MQ=255)
                                                 tGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTTCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGATGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTg                                                                                                                                                                                                 >  1:986713/1‑239 (MQ=255)
                                                 tGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTg                                                                                                                                                                                                 >  1:1116511/1‑239 (MQ=255)
                                                 tGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTg                                                                                                                                                                                                 >  2:1055877/1‑239 (MQ=255)
                                                 tGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTg                                                                                                                                                                                                 >  1:520524/1‑239 (MQ=255)
                                                    gcgTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGAt                                                                                                                                                                                               <  1:528843/238‑1 (MQ=255)
                                                        tCTTATTTTACTGTTTAGGCGCAGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATCTGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCg                                                                                                                                                                                                                                       <  1:844864/194‑1 (MQ=255)
                                                                tagggggtATGCGCCGCTGGCCGCAGCGCTGGTCGAGTGGCTACGGGAAATGCTGCCAGGTAAAACCACCGCGGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAg                                                                                                                                                                                <  1:5043/232‑1 (MQ=255)
                                                                tATGGTTTATGCGCCGCTGCCCGCAGCGCTTGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAg                                                                                                                                                                                <  2:1163484/239‑1 (MQ=255)
                                                                tATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAg                                                                                                                                                                                <  1:559520/239‑1 (MQ=255)
                                                                gatgtgttgtGCGCCGCTGCCCGCAGCTCTGGTCGAGTGGCTACGGGAAATTCGGCCAGGTAAAACCACCGCTGAATTGTAGATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAg                                                                                                                                                                                <  1:1163413/230‑1 (MQ=255)
                                                                  tGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTTGCTACGGGAAATTCTGCCTGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTTCCAGAAGCACGCCACAACCGAAAGCTACCGCGAATATCTCGTTTATCTCCAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGATATTCGTGGAATGGGGATGTTGGTGTTTACGCTGGCGGGCGTTGATCTG‑‑GTATTCAAAGtc                                                                                                                                                                              >  1:1115927/1‑239 (MQ=255)
                                                                  tGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTCTTCGTGGAATGGTg                                                                                                                                                                                                                             >  2:844864/1‑194 (MQ=255)
                                                                  tGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCAAGGTATAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACCCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGAGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGt                                                                                                                                                                               >  1:206278/1‑238 (MQ=255)
                                                                    gTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGCCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGACTATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGCGCTGTTGGTGTTTACGCTGCCGGGGTTTGATCGG‑‑GTATTCAACGtca                                                                                                                                                                             >  1:421489/1‑238 (MQ=255)
                                                                              cgcTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAgg                                                                                                                                                                  >  1:905760/1‑239 (MQ=255)
                                                                               gcTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGc                                                                                                                                                                                                          <  2:109613/200‑1 (MQ=255)
                                                                               gcTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGc                                                                                                                                                                                                          >  1:109613/1‑200 (MQ=255)
                                                                               gcTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGt                                                                                                                                                                 >  2:1113604/1‑239 (MQ=255)
                                                                                    ccGCAGCGTTGGTCGGGTTGCTACGGGAAATGCGGGCAGGTAAAAACACCGCTGAATTGTATATGGCTATCGGCTTCCAGCAGCGCGCCAAAACCGAAAGCGACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCATTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTAGTTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCACAGTCATCAAAGACAGGTTcgcg                                                                                                                                                            <  1:642228/239‑1 (MQ=255)
                                                                                           gcTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGcgccgc                                                                                                                                                        <  1:1111231/236‑1 (MQ=255)
                                                                                             tGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGGTGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACCGTGTCGCGCCGCAGaaa                                                                                                                                                   >  1:611632/1‑239 (MQ=255)
                                                                                             tGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGaaa                                                                                                                                                   >  1:79784/1‑239 (MQ=255)
                                                                                                        cTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                                                         <  2:905760/238‑1 (MQ=255)
                                                                                                                       ccAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                                                         <  2:1129663/239‑1 (MQ=255)
                                                                                                                        cAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                                                         <  2:770937/238‑1 (MQ=255)
                                                                                                                        cAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                                                         <  2:497619/238‑1 (MQ=255)
                                                                                                                        cAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                                                         <  2:1116511/238‑1 (MQ=255)
                                                                                                                        cAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                                                         <  2:520524/238‑1 (MQ=255)
                                                                                                                        cAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGACATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                                                         <  1:712755/238‑1 (MQ=255)
                                                                                                                             aaaaCCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCt                                                                                                                    <  1:50587/238‑1 (MQ=255)
                                                                                                                                  caccGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAAc                                                                                                              <  2:177142/239‑1 (MQ=255)
                                                                                                                                    ccGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCAACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCCAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCCCGTTCATGCATGCTATCAACTg                                                                                                            >  1:53331/1‑239 (MQ=255)
                                                                                                                                     cGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTgg                                                                                                           >  2:16959/1‑239 (MQ=255)
                                                                                                                                        tGAATTGTATATGGCTATCGGCTGACAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGa                                                                                                        <  2:499954/239‑1 (MQ=255)
                                                                                                                                                atatGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCAc                                                                                              >  2:782573/1‑239 (MQ=255)
                                                                                                                                                 tatGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCAc                                                                                              >  1:865765/1‑238 (MQ=255)
                                                                                                                                                  atGGCTACCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTAACGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGa                                                                                            <  2:583925/239‑1 (MQ=255)
                                                                                                                                                    ggCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGAt                                                                                           >  1:840712/1‑238 (MQ=255)
                                                                                                                                                    ggCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATc                                                                                          >  2:535400/1‑239 (MQ=255)
                                                                                                                                                    ggCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATc                                                                                          >  2:837167/1‑239 (MQ=255)
                                                                                                                                                      cTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCggg                                                                                                                                                                                                                                             <  2:556019/94‑1 (MQ=255)
                                                                                                                                                      cTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCggg                                                                                                                                                                                                                                             >  1:556019/1‑94 (MQ=255)
                                                                                                                                                      cTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAg                                                                                                                                                                                >  2:1168240/1‑153 (MQ=255)
                                                                                                                                                      cTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAg                                                                                                                                                                                <  1:1168240/153‑1 (MQ=255)
                                                                                                                                                      cTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATcgc                                                                                        >  1:132963/1‑239 (MQ=255)
                                                                                                                                                      cTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATcgc                                                                                        >  2:581464/1‑239 (MQ=255)
                                                                                                                                                         tccgCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGCGCACGACCGCGTg                                                                                     >  1:701345/4‑239 (MQ=255)
                                                                                                                                                         tCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGCTGTTGGTGTTTACGCTGCCGGGCTTTTATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGTATCCGTTCGTCCCGGCTATCAACTGGTGAC‑‑AGAGCACGATcaccag                                                                                     >  1:583925/1‑234 (MQ=255)
                                                                                                                                                         tCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTg                                                                                     >  2:821951/1‑239 (MQ=255)
                                                                                                                                                         tCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTg                                                                                     >  1:167945/1‑239 (MQ=255)
                                                                                                                                                         tCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCACGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATcgcg                                                                                       >  1:570845/1‑239 (MQ=255)
                                                                                                                                                               gCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGa                                                                               <  2:725751/239‑1 (MQ=255)
                                                                                                                                                                 cAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGaa                                                                              <  2:950035/238‑1 (MQ=255)
                                                                                                                                                                        aCGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCgg                                                                        <  2:79784/237‑1 (MQ=255)
                                                                                                                                                                          gCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                                                         >  1:48988/1‑172 (MQ=255)
                                                                                                                                                                          gCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                                                         <  2:48988/172‑1 (MQ=255)
                                                                                                                                                                                    aaaGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGttt                                                          >  2:848336/1‑239 (MQ=255)
                                                                                                                                                                                    aaaGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGAGGGCGGAATGGCGGACACACAGGAGttt                                                          >  1:920365/1‑239 (MQ=255)
                                                                                                                                                                                      aGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGa                                                        <  1:16959/239‑1 (MQ=255)
                                                                                                                                                                                      aGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGa                                                        <  1:821951/239‑1 (MQ=255)
                                                                                                                                                                                                       cGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGTGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGgaga                                       <  1:247868/239‑1 (MQ=255)
                                                                                                                                                                                                                  aGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCCCGTTCGTGCCTGCTATCAACTGGTGAC‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGCCACCCAGGAGTTTGCAAACTTTGTGCTGGAGAAGCGGCATAtt                            >  1:131734/1‑239 (MQ=255)
                                                                                                                                                                                                                  aGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATAtt                            >  2:1014973/1‑239 (MQ=255)
                                                                                                                                                                                                                    ggCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATAttt                           >  1:379584/1‑238 (MQ=255)
                                                                                                                                                                                                                    ggCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGTCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATAttt                           >  1:591100/1‑238 (MQ=255)
                                                                                                                                                                                                                     gCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCata                              <  2:611632/236‑1 (MQ=255)
                                                                                                                                                                                                                       tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATTTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTccc                        <  2:986713/238‑1 (MQ=255)
                                                                                                                                                                                                                       tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTccc                        <  2:973466/238‑1 (MQ=255)
                                                                                                                                                                                                                       tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTCTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGACAACTTTGTGCTGGAGAGGCGGCAGATTTcccc                       >  1:973466/1‑239 (MQ=255)
                                                                                                                                                                                                                         tAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCg                      >  1:769495/1‑238 (MQ=255)
                                                                                                                                                                                                                          aaTGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCgg                     <  2:132963/238‑1 (MQ=255)
                                                                                                                                                                                                                              agtaGTGCATTGAAGCGCCGGGTATTCGTGGAATGGGGATGTTGGTGTTTACGCTGCCGGGCTTTGAGCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAGCTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCAtt                 <  1:1055877/235‑1 (MQ=255)
                                                                                                                                                                                                                              aGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTa                >  2:490086/1‑239 (MQ=255)
                                                                                                                                                                                                                                   ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGa           >  2:557017/1‑239 (MQ=255)
                                                                                                                                                                                                                                   ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGa           >  1:853464/1‑239 (MQ=255)
                                                                                                                                                                                                                                   ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGa           >  1:1008485/1‑239 (MQ=255)
                                                                                                                                                                                                                                   ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGa           >  1:96971/1‑239 (MQ=255)
                                                                                                                                                                                                                                   ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGa           >  1:504088/1‑239 (MQ=255)
                                                                                                                                                                                                                                   ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGa           >  1:275003/1‑239 (MQ=255)
                                                                                                                                                                                                                                   ttCATTGAAGCGCCGGGGATTCGTGGAATGGTGATGTTAGTCTTTACGTTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGCATCGTGCCGCAGAAAGAGAGGTCTGCTAATTATGTTCGTGCCTACTCTCGACTGATTAA‑‑AGAGCCAGAACATGTGGGCCGTTTGGCGTTTACCCAGCAGGTTAAACAGTTAGTGATAGCGATGCGTCATATTTCCCCGGCATTAATGGa           >  1:907399/1‑239 (MQ=255)
                                                                                                                                                                                                                                   ctCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCACAGACAGGTTCGCGCCGCAGAAAGCGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTCTGAAAACGTTGGGCTGCAGAAGCGGCATATTTCCCCGGCATTCATGGa           >  1:87927/2‑239 (MQ=255)
                                                                                                                                                                                                                                     cATTGAAGTGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCACAGTCATCAAAGACAGGTTAGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGATTTTGAAAACTGTGTGCTGGAGAAGCGGCATATTTCCACGGCATTACTGGAAt         >  1:314465/1‑239 (MQ=255)
                                                                                                                                                                                                                                       ttGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTa       <  2:853464/239‑1 (MQ=255)
                                                                                                                                                                                                                                       ttGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTa       <  2:206278/239‑1 (MQ=255)
                                                                                                                                                                                                                                           aGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTg    <  2:840712/238‑1 (MQ=255)
                                                                                                                                                                                                                                           aGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTg    <  2:504088/238‑1 (MQ=255)
                                                                                                                                                                                                                                             cgcCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCt  >  2:119267/1‑238 (MQ=255)
                                                                                                                                                                                                                                             cgcCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCt  >  1:810704/1‑238 (MQ=255)
                                                                                                                                                                                                                                             cgcCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCt  >  1:851235/1‑238 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 
TTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGG‑‑GTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAA‑‑AGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCT  >  NC_000913/4219301‑4219775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: