Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,392,454 C→T 5.7% intergenic (+18/‑19) glyV → / → glyX tRNA‑Gly/tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,4540CT 5.7% 207.6 / 4.1 70intergenic (+18/‑19)glyV/glyXtRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (31/35);  new base T (2/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

CGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAAT  >  NC_000913/4392217‑4392674
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                            
cGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCa                                                                                                                                                                                                                             <  2:623195/239‑1 (MQ=255)
 gTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCaa                                                                                                                                                                                                                            >  2:450186/1‑239 (MQ=255)
   cTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCaaa                                                                                                                                                                                                                           >  1:1053010/1‑238 (MQ=255)
   cTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                                                                                                                                                                                                          <  1:572731/239‑1 (MQ=255)
   cTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                                                                                                                                                                                                          <  2:1176839/239‑1 (MQ=255)
   cTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTCCTGCaaa                                                                                                                                                                                                                           >  1:457894/1‑238 (MQ=255)
   cTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGATTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                                                                                                                                                                                                          >  2:802693/1‑239 (MQ=255)
    ttGCTGATAAGTTCAGCACTTTGGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGc                                                                                                                                                                                                                         <  1:432382/239‑1 (MQ=255)
     tGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGc                                                                                                                                                                                                                         >  2:312377/1‑238 (MQ=255)
     tGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGc                                                                                                                                                                                                                         >  2:1001875/1‑238 (MQ=255)
        tGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAGCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGcaca                                                                                                                                                                                                                      <  1:185309/238‑1 (MQ=255)
            aaTTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGacc                                                                                                                                                                                                                  >  2:63973/1‑238 (MQ=255)
              tttCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGaccacc                                                                                                                                                                                                               <  1:415799/239‑1 (MQ=255)
              tttCAGCACTCGAGATAAAAACGCAAAAAAAACTTTTTGGGGGGGTTTCAGAAGGAAAGATTTCTCGTATAATGCGCTCCCCTTAACGACGCAGAACTGCGAAACGTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGCCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGaccgcc                                                                                                                                                                                                               <  1:731130/239‑1 (MQ=37)
                      cTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCggg                                                                                                                                                                                                       >  2:307331/1‑239 (MQ=255)
                         gagaTAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGGTGCTGCAAAGCACAGGCAACCCACGCGGCAAt                                                                                                                                                                                                    >  1:360618/1‑239 (MQ=255)
                         gagaTAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAAt                                                                                                                                                                                                    >  2:1041284/1‑239 (MQ=255)
                             tAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGc                                                                                                                                                                                                 <  1:63973/238‑1 (MQ=255)
                             tAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCt                                                                                                                                                                                                <  2:412906/239‑1 (MQ=255)
                              aaaaaCGCAAAAAAATATTTTTTGGGAGGGTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCATAAATGCGAAAAGTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTGCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTc                                                                                                                                                                                               <  1:44971/239‑1 (MQ=255)
                                 aaCGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCTCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGt                                                                                                                                                                                            <  2:1083363/239‑1 (MQ=255)
                                    gCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGACTCCCGTAACGACGCAGAAATGCGAAAATTACGAAATCAAAATTAAGTAGTACGCAGGAATAGCTCAGTTGGTAGAGGACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                                                                                                                                                                                         <  2:256734/239‑1 (MQ=255)
                                               ttttttGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAc                                                                                                                                                                              <  2:14487/239‑1 (MQ=255)
                                                tttttGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACATCCCAAGAGGGAATCGCTCGGTTGGAAGAGCACGa                                                                                                                                                                               >  2:1118489/1‑237 (MQ=255)
                                                   ttGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTg                                                                                                                                                                          <  1:1001875/239‑1 (MQ=255)
                                                     ggggggTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc                                                                                                                                                                        <  1:312377/239‑1 (MQ=255)
                                                     ggggggTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc                                                                                                                                                                        <  2:373606/239‑1 (MQ=255)
                                                                   ggAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                                                                                                                                                          >  1:933471/1‑239 (MQ=255)
                                                                             ctcGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTGAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCa                                                                                                                                                                                                             >  1:751834/1‑177 (MQ=255)
                                                                             ctcGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTGAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCa                                                                                                                                                                                                             <  2:751834/177‑1 (MQ=255)
                                                                                      tGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                                                                                        >  2:566705/1‑238 (MQ=255)
                                                                                        cgcCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                                                                                      >  2:574314/1‑238 (MQ=255)
                                                                                           cTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCt                                                                                                                                   >  2:958206/1‑238 (MQ=255)
                                                                                           cTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGc                                                                                                                                                                                   <  2:461150/189‑1 (MQ=255)
                                                                                           cTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGc                                                                                                                                                                                   >  1:461150/1‑189 (MQ=255)
                                                                                           cTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACATTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGCAAAGTGCTGCAAAGACCAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTGCCGTTCCCCGCt                                                                                                                                   >  2:879893/1‑238 (MQ=255)
                                                                                                     gaagaaGAAATGCGAAAATTAAGAAAGCCAGATATAGTAGTACAAAGGAAAAGCTCAGTTGGTAGAGCACTACCATGCCAAAGGAGGGGTCGCGAAATCGAGTCTCCGTACCCGCGCAAAAATGTGAAAAGTACTACAAAGCACAGAACGCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGa                                                                                                                        <  1:293966/234‑1 (MQ=14)
                                                                                                       cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAATGTGTTGCAAAGCCAAGACCACCCGAGCGGTAATTGCTCGGTTGGTATGTCTCGACCTAGCCAAGGTAGGGGTCGCGAGTCATAGCCTCGTTTCCCGCCCCAAACttttaca                                                                                                                      >  1:279820/1‑235 (MQ=17)
                                                                                                       cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaa                                                                                                                      >  2:399805/1‑239 (MQ=255)
                                                                                                       cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaa                                                                                                                      >  2:1099632/1‑239 (MQ=255)
                                                                                                       cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaa                                                                                                                      >  2:1156707/1‑239 (MQ=255)
                                                                                                       cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGAGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGACGGGGTCGCGAGTACGAGTCTCGTTTCCTGCTCCAAAATTTGaca                                                                                                                      >  1:924111/1‑237 (MQ=255)
                                                                                                        gCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAg                                                                                                                     >  1:1184432/1‑239 (MQ=255)
                                                                                                             aaTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg                                                                                                                   <  2:924111/236‑1 (MQ=255)
                                                                                                              aTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt                                                                                                               <  1:574314/239‑1 (MQ=255)
                                                                                                                        tACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGa                                                                                                     <  2:1184432/239‑1 (MQ=255)
                                                                                                                        tACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGa                                                                                                     <  2:457894/239‑1 (MQ=255)
                                                                                                                             aaGAAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGaccacc                                                                                                <  2:322612/239‑1 (MQ=255)
                                                                                                                                  aatcaaaGGAGGACGCGGGAATAGATTATTTGTTGGGGCATGGCCGTGCGAAGGTCTGGGTAGAAAATGCGAGTCCCGTTTCCCGCTCAAAAAGTTGAAAAGTTCTGCAAAGAACTAACCAGCCAAGCGGGAATAGCTCAGTTGTTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGc                                                                                           <  1:307331/234‑1 (MQ=17)
                                                                                                                                  aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGc                                                                                           <  2:933471/239‑1 (MQ=255)
                                                                                                                                       aggtaataCGATGGAGTAGCACAGTTGGTAGAGGACTACCTAGCGAAGGGAGGGGTCGCGAGTGCGAGTCTCGTTTCCCGCGCAAAAATGTGAAAAGTGTTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGaa                                                                                      <  1:595894/237‑1 (MQ=17)
                                                                                                                                            gtaCGCGGGAAAAGCGCAGTTGGTAGAGCACGGCCTGGACAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGc                                                                                  <  1:1156707/238‑1 (MQ=255)
                                                                                                                                              aCGCGGGAATAGTGCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCa                                                                               <  2:303714/239‑1 (MQ=255)
                                                                                                                                              aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGTGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCt                                                                                 <  2:314946/237‑1 (MQ=255)
                                                                                                                                               cgcgGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt                                                                                                               <  2:1019424/206‑1 (MQ=255)
                                                                                                                                               cgcgGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTACAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCa                                                                               <  2:490206/238‑1 (MQ=255)
                                                                                                                                               cgcgGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATACCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt                                                                                                               >  1:1019424/1‑206 (MQ=255)
                                                                                                                                                    gAATAGCTCAGTTGGTAGAACACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                                                                          <  2:1098308/238‑1 (MQ=255)
                                                                                                                                                    gAAAAGGTCATTTGGTGGAGGACGACCTTGCCAAGGACTGGGTCGCGAGTTGGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                                                                          <  1:1185411/238‑1 (MQ=18)
                                                                                                                                                               ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGACACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGa                                                                >  2:954501/1‑237 (MQ=255)
                                                                                                                                                                       gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                      >  2:1066792/1‑239 (MQ=255)
                                                                                                                                                                                 gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGGGCACGCCCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAAACCACACAAGTGGGAATGACTCAGTTGGTAGATAACAGCCATACCAAGGTAGCGGTcg                                            >  1:1158279/1‑239 (MQ=17)
                                                                                                                                                                                       gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGCAACTACTTTAAAGCACAGACCACCCCACCGGGCATTTATCAGTTGGTCGAGCACCGCCTTGCCCAGCTCGGGGtggtgtggt                                      >  1:54647/1‑231 (MQ=17)
                                                                                                                                                                                       gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCCCGGTTGGTAGAGCACGCCCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGACAGTGCTGAAAGGCCAAGACCACCCAATCGGGTATAGCTCAGTTTGTACAGCACGTCCGTGCCAAAGCCGGGGTCCAGAGt                                       >  1:431956/1‑238 (MQ=18)
                                                                                                                                                                                           gggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGCAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAg                                  >  2:734640/1‑239 (MQ=255)
                                                                                                                                                                                           gggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGCAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGATCACGACCTTGCAAAGCTCGGGGGCGGGAGTTCgcg                                  >  2:1070374/1‑237 (MQ=255)
                                                                                                                                                                                                               gTTTCCCGCTCCAAAATTTGCAAAGGGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGACCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAACTTGCTGAAAGACACAGACCACCCAAGCGGGAATAGCTCAGTTGTTAGAGCACGACCTTGCCAACGTCGAGGCCGCGATTTCGAGTCGCGTTTccattcacacat              >  1:377188/1‑229 (MQ=17)
                                                                                                                                                                                                                     tcgaacAAAAGTGGAAAAGTGCTGCAAAGCATGGACAACACAAGCGGGAATAACACATTGGGTAGAGCGCTGCATTGGCAAGGCTGGGGCGTCGAGTTCGAGTCCCGTTTCCCGGTCCAAAATTTGAAAGTGCGGTAAGGCACAGACCTACCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATtcttct        <  1:1118489/234‑1 (MQ=17)
                                                                                                                                                                                                                        tCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctct      <  1:1066792/238‑1 (MQ=255)
                                                                                                                                                                                                                           aaaaTTTGAAAAGTGCTGCAAAGCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAAt  <  2:948818/239‑1 (MQ=255)
                                                                                                                                                                                                                                             cAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctctc     <  2:686406/218‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                            
CGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAAT  >  NC_000913/4392217‑4392674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑