Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 375,247 A→G 4.7% intergenic (+366/‑212) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913375,2470AG 4.7% 297.5 / 2.8 98intergenic (+366/‑212)mhpE/mhpT4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (40/51);  new base G (3/2);  total (45/53)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

CGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCT  >  NC_000913/374932‑375532
                                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                
cGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAG‑‑AT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑AACGTCTTGTCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTa                                                                                                                                                                                                                                                                                                  <  2:254898/288‑1 (MQ=11)
                            ctgtgggtggcaggaggATTTGTAGGTCTGATAAGGCGCGTTGGCGTCGCAGCAGGCAGCTGCGCACGACTGAGGGATGGGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAGGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTa                                                                                                                                                                                                                                                                                                  <  1:662099/275‑1 (MQ=14)
                                    ggCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTa                                                                                                                                                                                                                                                                                         >  2:157471/1‑289 (MQ=255)
                                     gCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTAGCATCAGGCACCTGCGCACGACTTCCGGATGCGGCTAAAACGCCTTa                                                                                                                                                                                                                                                                                         >  1:1124229/1‑288 (MQ=255)
                                          agaATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACTGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                                                                                                                                                                    >  1:893440/1‑288 (MQ=255)
                                                tGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTCTCCGGCCTACGGGTGGCGCCAGAATTTGTAGGCCGGCTAAGACGCGTTAGCGTCGCATAAGGCATCTGCGCACGAAGGCCGGGAGCGGCTGTAACCCCCTATCCCGccaaagg                                                                                                                                                                                                                                                                             >  1:473430/1‑284 (MQ=14)
                                                tGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACgg                                                                                                                                                                                                                                                                             >  2:151740/1‑289 (MQ=255)
                                                      gcggATAACACCGGTTAACGTCGGACCAGCGGTCTGCGCACGACGGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAGGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGGGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGgcg                                                                                                                                                                                                                                                                       <  1:1134541/286‑1 (MQ=14)
                                                        tGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTTGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGAATCAGGCATTCGCGCCAGACTGCCGGGTGCGGCGTCAATGCCTTTGCCGGCCTACggccggcccg                                                                                                                                                                                                                                                                     >  1:506173/1‑281 (MQ=14)
                                                           taagagtggTTAGGGTGGGAGCAGGCATGTGGGCAGGGCTGCCGGAGGGGGCGGGAGCGCCTTCTCCGGCGTACGGATGGCTGGGGAATTTGTAGGCCTGATAAGACGCGTTGGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCggg                                                                                                                                                                                                                                                                   <  1:522489/280‑1 (MQ=14)
                                                               gcgcgTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAAt                                                                                                                                                                                                                                                                <  1:151740/286‑1 (MQ=255)
                                                                cgcgTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCCTCAGGCATCTGCGCACGACTTCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAACTTg                                                                                                                                                                                                                                                             >  2:945500/1‑289 (MQ=255)
                                                                  agtgaGCGTCGCATCAGGCATCTGCGAACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCGTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGAGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGt                                                                                                                                                                                                                                                            <  2:590962/284‑1 (MQ=255)
                                                                    ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGGTAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCGTCTCCGGCCTACGGATGGCGGGGGAATTTGTAgg                                                                                                                                                                                                                                                         >  1:832855/1‑289 (MQ=255)
                                                                    ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATATGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAGTCCCTTCTGCCGCCTACGGAGGCCGCGGGAATTTGtgg                                                                                                                                                                                                                                                          >  1:386131/1‑286 (MQ=14)
                                                                             caaattcccgcgccatccgtaggCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACGGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGCAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAg                                                                                                                                                                                                                                                >  2:983589/23‑289 (MQ=255)
                                                                             cATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGGGTGGCGCGAGAACTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTCCGGATGGCGCGGGAATTTGTAGGCCTGATAAg                                                                                                                                                                                                                                                >  2:667221/1‑289 (MQ=255)
                                                                             cATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATATGCGCACGACTGCAGGATGCGGCGTAAACGCCTTATCCGGCCTATGGACGGCGCGGGAATTTGTAGGCCGGATAAg                                                                                                                                                                                                                                                >  2:643425/1‑289 (MQ=255)
                                                                              aTCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGa                                                                                                                                                                                                                                               <  2:657115/289‑1 (MQ=255)
                                                                                             aCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGCTAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGAATGCCGGATGCTGCGTAAACGCCTTATCCGGCCTACGGCTGGCGCGGGAATCTATAGGCCTCATAAGACGCGTGAGAGTCGCa                                                                                                                                                                                                                               >  2:938082/1‑289 (MQ=255)
                                                                                                    ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGGAGGCCTGATAAGAGGCGCTAGGGTCGCATCAGGCa                                                                                                                                                                                                                        >  1:1110127/1‑289 (MQ=255)
                                                                                                    ccGGATGCGGCGTGAACGCCTTATCCGGCCTAAGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGGTAAGAGGCGTTAGCGGCGCATCAGGCa                                                                                                                                                                                                                        >  2:98403/1‑289 (MQ=255)
                                                                                                     cGGGTGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGGCGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCAt                                                                                                                                                                                                                       <  2:1207119/289‑1 (MQ=255)
                                                                                                                            acGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGTTAAGACGCGTTAGCCTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCAGATGCG‑‑a                                                                                                                                                                                              <  2:9260/288‑1 (MQ=255)
                                                                                                                               gCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATaa                                                                                                                                                                                           >  2:1053504/1‑289 (MQ=255)
                                                                                                                               gCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGCAAACGCCTTATCCGGCCTACGGATCGCGCGGGCATTTTTCGGCCTGATTAGACCCGTTATCGTCCCATCCGCCTACCGGACACGACTGCCGGagtctatca                                                                                                                                                                                             >  1:478149/1‑281 (MQ=14)
                                                                                                                                ccTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCGGGACACGAATGCCGGATGTG‑‑ATaaa                                                                                                                                                                                          >  2:1044164/1‑289 (MQ=255)
                                                                                                                                ccTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGGCGGCAAAAACCCGTTAGGCGGCCAACAGGCATCTAcaaccggcatgcgggctgcgtata                                                                                                                                                                                            >  1:584848/1‑267 (MQ=14)
                                                                                                                                   aCGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGCATTTGTATGCCTGACACGACTCGTTAGCGTCCCCTCCGCCATCTGCACCCCACCGCCGGATCCG‑‑CTatcac                                                                                                                                                                                        >  1:63729/1‑284 (MQ=14)
                                                                                                                                        tgcgccgGGAATTTGTAGTCCTGATACGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCACATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGt                                                                                                                                                                                  <  2:465300/284‑1 (MQ=255)
                                                                                                                                        tGGCGCGGGAATTGGTAGGCCTGATAAGACGCGTTAGCGTCGCAGCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGt                                                                                                                                                                                  <  2:535526/289‑1 (MQ=255)
                                                                                                                                         ggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGGCGCATCAGGAATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGt                                                                                                                                                                                  >  2:704707/1‑288 (MQ=255)
                                                                                                                                         ggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAAGTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACTCGTTAGCGTCCAATCAGGCATCTGCACCCGACTGCCGGCTGCG‑‑ACAACCGtacttg                                                                                                                                                                                  >  1:1204074/1‑283 (MQ=14)
                                                                                                                                              tggAATTTGTAGGCCTCATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGcc                                                                                                                                                                            <  2:120181/288‑1 (MQ=255)
                                                                                                                                               gtgaTTTGTATGCCTGATAAGACCCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGGGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGCGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCGTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGcc                                                                                                                                                                            <  1:157471/285‑1 (MQ=255)
                                                                                                                                                     tGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTCCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATa                                                                                                                                                                                            <  2:630362/266‑1 (MQ=255)
                                                                                                                                                           ccTGATACGACGCGTTCGCGTCGCATCAGGCATCTGCGCACGACTGCCGGAGGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGCGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCATGCATCTGCGCACGACTGCCGGATGCCGCGTAAACGCATTATCCGGCCTAAGTATGGCGAGGGAATATGTAGGCGTGATAAGACGAGTTAGCGTTGCATCAGTCCTGTGCGAACGACTGCCGGATTCT‑‑ATAAACGGACTTCCCGGCCTCCATTTCGCCCg                                                                                                                                                               >  1:71623/1‑289 (MQ=11)
                                                                                                                                                             tGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACCCGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCATACCTTTCGCCCGTa                                                                                                                                                             >  1:546170/1‑289 (MQ=255)
                                                                                                                                                               aTAAGACGCGTTAGCGTCGCATCAGGCATCTCCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGGATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCCGCCCACGACCTCCGGAAGCG‑‑ATCAACGTCTTGTctcgtctcttacacacatctga                                                                                                                                                            >  1:630362/1‑267 (MQ=14)
                                                                                                                                                                taggACGCGTTGGCGTCGCATCAGGCAGCGTCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTGGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                                                                                                                          <  2:815022/286‑1 (MQ=255)
                                                                                                                                                                tAAGACGCGTTAGCGTGGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                                                                                                                          <  2:768163/289‑1 (MQ=255)
                                                                                                                                                                  aGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAg                                                                                                                                                        <  2:893440/289‑1 (MQ=255)
                                                                                                                                                                     cgcgTTGGCGTGGCATCAGGCATCTGCGCAGGACTGCCGGATGGGGCGGGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                                                                                                                     <  1:983589/289‑1 (MQ=255)
                                                                                                                                                                     cgcgTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                                                                                                                     <  2:1110127/289‑1 (MQ=255)
                                                                                                                                                                      gggttcgagTCGCATCAGTCATGTGCGTGTGACTGCCGGGTGCGGCGTGAACGCCGTAGCCGGCATACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGGGCGGGAATTTGTAGGCATGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                                                                                                                     <  2:553228/280‑1 (MQ=17)
                                                                                                                                                                      gcgTTAGCGTCGCAGCAGGCATCGGCGCACGACTGCGGGATGGGGCGTGAACGCCTGAGCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                                                                                                                     <  1:727721/288‑1 (MQ=255)
                                                                                                                                                                         ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACg                                                                                                                                                                                                                                                                              >  2:369628/1‑167 (MQ=14)
                                                                                                                                                                         ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                                                                                                                                                                                                                                                                              <  1:369628/167‑1 (MQ=35)
                                                                                                                                                                         ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTCTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGCATTTATAGGCCTGATACGACGCGCTAGCGTCGCATCCAGCATCTGCACACCACTGCCGGATGCG‑‑ACAAACGTCCTGTTCTGCCACCATTTCGCCCGttgctcgttcttac                                                                                                                                                 >  1:836108/1‑276 (MQ=14)
                                                                                                                                                                          ttgCGTCTCATCAGGCATCTGCGGACGACTGCCGGAGTCGGCGTGACCGCCGTTTCCGGCCTACGGTTGGCGCGAGAATTTGTAGGCCTGAGAAGAAGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACTCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa                                                                                                                                                <  1:957970/287‑1 (MQ=17)
                                                                                                                                                                          tAGCGTGGCATCAGTCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa                                                                                                                                                <  2:584848/289‑1 (MQ=255)
                                                                                                                                                                          tAGCGTCGAATCAGGCATCTGCGGAAGACTGCCGGATGCGGCGTGAACGTCTTATCCGGCCGACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa                                                                                                                                                <  2:473430/289‑1 (MQ=255)
                                                                                                                                                                                            ttgccagcgcgtGCCGGATGGGGCGTGATCTCGTGATCGGGCCTAGTGGGTGCAGTGGAATTGGTGGGACTGACAGGACGCGTTGGCGTCGCATCAGGCATCTGCGCACGACTGCCGGTTGCGGCTGAAACGCCTTGTCCGGCCTACGGATGGCGCGGGAATTGTTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCgg                                                                                                                               <  1:938082/277‑1 (MQ=18)
                                                                                                                                                                                                 cACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGc                                                                                                                          <  1:1053504/288‑1 (MQ=255)
                                                                                                                                                                                                  aCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCaa                                                                                                                        <  2:503097/289‑1 (MQ=255)
                                                                                                                                                                                                      cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCCTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCTTTACCTAGTTCTGATTACGCGGCTGGCAATGTa                                                                                                                    >  1:1156036/1‑289 (MQ=255)
                                                                                                                                                                                                       tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTAt                                                                                                                   <  2:832855/289‑1 (MQ=255)
                                                                                                                                                                                                         ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTTTCAGGCCTACATTTCACCCGGAGGCAGTCATTAAATCATTCGGATTAAGCGGCTGGCACTGTATCa                                                                                                                 >  2:48776/1‑289 (MQ=255)
                                                                                                                                                                                                           ggATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGt                                                                                                               <  2:1142845/289‑1 (MQ=255)
                                                                                                                                                                                                            gaggtggcgCGAACGCCTTATCCGGACTACTGGGGGCGGGGGAAGTTGTAGGCCGGATAAGACGCGTTAGCGTCGCATCAGGAATCGGCGCACGACTGCCGGATGCGGCGGAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCGGATAAGACGCGTTAGCGGCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGt                                                                                                               <  1:76124/283‑1 (MQ=18)
                                                                                                                                                                                                            gATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGt                                                                                                               <  1:653517/288‑1 (MQ=255)
                                                                                                                                                                                                            gATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGAACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGt                                                                                                               <  2:546170/288‑1 (MQ=255)
                                                                                                                                                                                                                cggcgTAAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAATACGCGTTAGCGTCGCATCAGGCATCTGCACACCACTGCCGGATGCG‑‑ATACACGTCTTGTCCGGCCTACATTTAGCCCGCAGGCAGTCCTCAACTAGTTCTGATTACTCGGCTGGCAATGTTTCTGTcgcgg                                                                                                          >  1:946474/1‑288 (MQ=18)
                                                                                                                                                                                                                   cgTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑AGAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATCGTTCTGATTACTCGGCTGGCAATGTATCAGGCGCGAtt                                                                                                        >  2:643132/1‑288 (MQ=255)
                                                                                                                                                                                                                        aCGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTg                                                                                                  <  2:1124229/289‑1 (MQ=255)
                                                                                                                                                                                                                           ccTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCAc                                                                                               <  2:131163/289‑1 (MQ=255)
                                                                                                                                                                                                                               aTCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                                                                                                                                                                                        <  1:142244/103‑1 (MQ=40)
                                                                                                                                                                                                                               aTCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                                                                                                                                                                                        >  2:142244/1‑103 (MQ=40)
                                                                                                                                                                                                                                  cgcccgaCGGGTGGCGCGAGAATTTGGAGGCCGGATAAGACGCGTTAGCGTGGCATCAGGCATCTGCGCACGGCGGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAAc                                                                                        <  1:643132/283‑1 (MQ=255)
                                                                                                                                                                                                                                    gCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCGGGCCTACATTTCGTCCGTAGGCAGTCTTTAAATAGTTCTGATTACGCGGCTGGCAATGTCTCAGTCGCGTTTCACCTTCCCTCGCAACgt                                                                                      >  2:79716/1‑288 (MQ=255)
                                                                                                                                                                                                                                    gCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACg                                                                                       <  2:40506/288‑1 (MQ=255)
                                                                                                                                                                                                                                       tACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGt                                                                                   <  2:715188/289‑1 (MQ=255)
                                                                                                                                                                                                                                        tacggtgtgggcgAGATTTTGTAGGCCTGATAAGACGCGTTAGGGTCGCTTCAGGCATCTGCGCACGACGGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGt                                                                                   <  1:263848/279‑1 (MQ=255)
                                                                                                                                                                                                                                          cGGGTGGTGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGt                                                                                   >  2:12882/1‑287 (MQ=255)
                                                                                                                                                                                                                                          cGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCCCCCCTAGGCAGCCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGTCTCACCTGCACTCGCAACGAGGtt                                                                                  >  2:910955/1‑288 (MQ=255)
                                                                                                                                                                                                                                           gggTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGAGGGAATTTGTAGGCCTTATAAGACGACTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGTCAGCCATTAAATAGTTCTGATTACGCGGCCGGAAACGTATCAGTCGCGATTCACCTGCACCCGCAACGAGGTTCa                                                                                >  2:975291/1‑289 (MQ=21)
                                                                                                                                                                                                                                                gcgcGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTa                                                                           >  2:331941/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   cgAGAATTGGTAGGCCTGATATGACGCGTTAGCGTGGCATCAGGCATCTGTGTACGACTGCCGCATGCGGCGTAAACGCCTTATCCGGCATACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATg                                                                         <  2:590792/288‑1 (MQ=255)
                                                                                                                                                                                                                                                    gagaATTTGTAGGCCTGAGAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACTACTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATATTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGt                                                                        <  2:834612/288‑1 (MQ=255)
                                                                                                                                                                                                                                                          aggTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCAACTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTAGCCGGCCTACGGATGGCGCGGGAATGTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCg                                                                 <  1:79716/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTCCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCgg                                                                                                                               <  1:636631/219‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTCCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCgg                                                                                                                               >  2:636631/1‑219 (MQ=255)
                                                                                                                                                                                                                                                                    gATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGcc                                                                                                                                                                 >  1:62881/1‑183 (MQ=255)
                                                                                                                                                                                                                                                                    gATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGcc                                                                                                                                                                 <  2:62881/183‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTtcat                                                      <  2:63729/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ggcgGTAGCTTCGCATGAGGGCTCGGCGAACACGGGCGGGCTGGGGCGTAGACGGCTTTGCCGGCCTACGGATGGGGCGGGGATTTGTAGTCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCatcatc                                                  <  1:643425/286‑1 (MQ=37)
                                                                                                                                                                                                                                                                               ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑a                                                                                                                                                                                              <  2:401080/143‑1 (MQ=35)
                                                                                                                                                                                                                                                                               ttAGCGTCGAGTCAGGCATTGGCGTACGACGGCCGGATGCGGCGTAAACGCCTTATCCGGCCGACGGATGGCGAGGGAAGTTGTAGGCCTGATAAGAGTCGTTAGCGTCGCATCAGGCATCTGCACACGACGGCCGGATGCG‑‑ATAAACGTCGTGTCCGGCCGACATTTCGCGCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATc                                            <  1:945500/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                tAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATc                                            <  2:506173/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         aTCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATg                                  <  2:668765/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                     cgcACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTtcat                                                      <  1:794041/257‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                     cgcACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTtcat                                                      >  2:794041/1‑257 (MQ=255)
                                                                                                                                                                                                                                                                                                     cgcACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCAACCCGCCTGATGCTGACCATCggg                      >  1:498535/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                                             tGCCGGATGGGGCTTAAACGCGTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTGGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTGCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGttt              <  1:667221/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                             tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGCGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGt                >  2:567740/1‑289 (MQ=25)
                                                                                                                                                                                                                                                                                                               ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCt                    >  1:246458/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                                                               ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCt                    <  2:246458/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                               ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATATATCAGt                                                                                                               >  1:911218/1‑190 (MQ=255)
                                                                                                                                                                                                                                                                                                               ccGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATATATCAGt                                                                                                               <  2:911218/190‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                                         cgTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGctct  >  1:126413/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                
CGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCT  >  NC_000913/374932‑375532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: