Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	375,100:1	+C	9.1%	intergenic (+219/‑359)	mhpE → / → mhpT	4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	375,100	1	.	C	9.1%	128.4 / 11.6	44	intergenic (+219/‑359)	mhpE/mhpT	4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base . (21/19);  new base C (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-02

GGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGT  >  NC_000913/374833‑375356                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                 ggaggaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAAGGATGGCGTGAGCATTTGGA‑GGTATGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGGACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAGGGATGGCGCGGGCAGTTGTGGGGCTGATCAGAAGGG‑TTAGCGt                                                                                                                                                                                                                                                           >  1:127108/1‑275 (MQ=21)   agggTATGATCGTTGACCGGGCGCTGGATCTGCGCAACAACAAATAGTGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGt                                                                                                                                                                                                                                                           <  2:502627/273‑1 (MQ=255)                tGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTa                                                                                                                                                                                                                                                               >  1:502628/1‑256 (MQ=255)                                    gcAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATACGGCCGACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TGAGAGTCGCATCAGGCATCTGCGCACGACTGCCGGATgcg                                                                                                                                                                                                                         >  1:178540/1‑274 (MQ=255)                                         aacaaATAATGAGGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGa                                                                                                                                                                                                                   <  1:354520/275‑1 (MQ=255)                                            aaaTAAGAATCACTGCCGACAACGTGCCTTTGCTAACCCCATCCATGCACGACTGCCAGATGCGTATTGACCGCCTTATCCCGCCTACGCATCGCGTCAAAATTTGTA‑GGTCTGTTAAGACGCGTTAGCTTCCCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAAc                                                                                                                                                                                                                 <  2:204023/274‑1 (MQ=14)                                                    gaggcggcgggggacggggaTTTGGTGTGCCCATCACATCGCGGCGGCCCGAGGCGGCGTGACCGCCGTGTGGGGCGTACGGATGGCGGGAGAATTGGGA‑GGGCTGATAAGACGCGTTGGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                                                                         <  2:135266/255‑1 (MQ=11)                                                    atgcCTGCCGAGAACGTGCATTTTGTATGCCCAACCCAGCACGACTGCCGGATGCGGCGTGAACGCCGTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                                                                         <  1:115485/274‑1 (MQ=255)                                                         tGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGAATACGGCATCTGCGAACGACTGCCGGAGGCGGCGTGGACGCCTTATCCGGCCt                                                                                                                                                                                                   >  1:374258/1‑275 (MQ=255)                                                                aaCGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGACTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCG‑TTAGCGTAGCATCAGGAATCTGCGCACGACTGCCGGATGCGGCGTGAACGCGTTATCCGGCCTACggggt                                                                                                                                                                                            >  1:527800/1‑273 (MQ=255)                                                                    tggatgtggtgtGCCCATCCCAGCACGGCGGCCGGATGCGGAGTGAGCGCCTTATCCGGCCTACGGATGGCGGGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg                                                                                                                                                                                         <  2:528819/263‑1 (MQ=255)                                                                     gCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTCGCGTCGCATCAGGCAGCTTCGTCCGACGGCCGGACGCGGCGTTGACCACTTGTCCGGCCTACGGGTGgcg                                                                                                                                                                                         >  2:126201/1‑273 (MQ=14)                                                                           tGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCCCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGACCGACTGCCGGATGCGGCGTGAACCCCCTCTCCGGCCTACAGGTTGCGCGGGAAtt                                                                                                                                                                                 >  2:293008/1‑275 (MQ=255)                                                                                   cagcccAGCACGACGGCCGGATGCGGCGTGATCGCCGTATCCGGCCGACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGc                                                                                                                                                                          <  1:271318/271‑1 (MQ=255)                                                                                     tCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGAGGAGTGAACGCCTTATCAGGACTACGGATGGCGCGGGAATTGGTAGGCGTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGACGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAGTGTGTAGGCCTg                                                                                                                                                                       >  1:69618/1‑275 (MQ=255)                                                                                                cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGAACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGTACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGCGTGGCGCGAGAACTTGTAGGCCTGATTAGACGCGt                                                                                                                                                            >  1:454958/1‑275 (MQ=255)                                                                                                     ggATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGTGAGAATTTGTG‑GGTCGGATAAGACGCGTTAGCGTGGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCg                                                                                                                                                       <  2:32188/275‑1 (MQ=255)                                                                                                      gagggggatggagcgcctgtgccGGCCTACTGAGGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGt                                                                                                                                                      <  2:178539/254‑1 (MQ=14)                                                                                                          cggggTGAACGCCTTATCCGGCCTACGGATGGCGGGAGAATTTTGA‑GGTCTGGTAGGACGCGTTAGGGTCGCATCAGGCACCTGCGCCCGACTGCCGGATGCGGCTTGAACGCCTTATCCGGCCTATGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCa                                                                                                                                                  <  1:126202/275‑1 (MQ=11)                                                                                                            gcgTGAACGCCTTTCGCGGCCTAGGCATGGCGTG‑GCAATTGTAGGGTCTGATAAGACGCGTTAGCGTCTCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCAt                                                                                                                                                 <  2:69617/274‑1 (MQ=11)                                                                                                             cgTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGCATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGAATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGGGTGGCGCGAGAGTTTGTAGGCCTGATAAGACGCGTAAGCGTCGCATcg                                                                                                                                               >  1:368094/1‑274 (MQ=255)                                                                                                                                        ggCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTCTCCGGCCTACGGCTGGCGCGAGACGTTTTAGGCCTGATAAGACGCCGTAGCGTCGCATCTGGCATCTGCGCTCGACTGCCGGCTgcg                                                                                                                    >  2:503621/1‑275 (MQ=14)                                                                                                                                        ggCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGAGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAAGCCTGATAAGACGCGTTAGCGACGCATCAGGCATCTGCGCCCGACGGCCGGAcgcg                                                                                                                    >  1:283765/1‑275 (MQ=255)                                                                                                                                                               aTAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTTGGCACCACTGGCGGATTCGCAGTAAACGCCTTAGCCgccat                                                                                              >  2:163342/1‑271 (MQ=14)                                                                                                                                                                              gcggcggccggcagcgggggaCGACTGGCGGCTGCGGCGGTATCGCCTTATCCGGCCTACGGTGGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGGACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGa                                                                               <  2:368093/255‑1 (MQ=14)                                                                                                                                                                               tCGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGt                                                                                                                                                                                             >  1:120075/1‑163 (MQ=11)                                                                                                                                                                                cGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCgg                                                                                                                         >  1:76564/1‑230 (MQ=11)                                                                                                                                                                                   aTCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg                                                                                                                                                                                         <  2:120074/163‑1 (MQ=11)                                                                                                                                                                                    tCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATgc                                                                                                                     <  2:76563/230‑1 (MQ=11)                                                                                                                                                                                                      cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATCAGACGCGTTAGCGTCGCATCCGGCATCTGCGCACGACTGCCGGCTGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAAGTTGTAGGCCTggtcaggagcgt                                                       >  1:307007/1‑264 (MQ=255)                                                                                                                                                                                                         ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCGTAATAAGACGCGTTAGCGTAGCATCAGGCATCTCCGCACGACCGCCGGATTAGGCGGAAACGCCTCATTCGGCCTACTGACACCGGGGGAATTCATAGCACTGATAAGGCGCGGTa                                                     >  2:474311/1‑274 (MQ=14)                                                                                                                                                                                                              tGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTGAACCGGCCTACGGGTGGCGCGAGAATTTGTCGGCCTGATAAGACGCGTTAGCGTCACGTCAGGCATCTACGCACGACTGCCGGATGCGGCGTAAGCGCCTTATCCGGACTACGGATGGAGCGGGAACTTGTAGGCCTGATAAGACGCGTTCGCGCCg                                               >  1:525121/1‑275 (MQ=14)                                                                                                                                                                                                                       aaccccgtgtgcggcgggcGGAGGGCGGGGGAATTTGGAGGCCGGAGAAGACGAG‑TTAGAGTCGCGTCAGGCATGTGCGCACGACTGCCGGATGCGGCGTGGACGCCTGATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGc                                       <  2:499768/256‑1 (MQ=11)                                                                                                                                                                                                                               ctCCGGCCTTCGGATGGCGCGGTAATTTGTAGGCCTTATAAGACGCT‑TTAGCTGCGCATCAGGCATCTGCGCACGACTGCCGGATGCGTCGTGAACGCCTTATCCTGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGcaca                              <  1:461707/274‑1 (MQ=255)                                                                                                                                                                                                                                             tGGCGCGGGAATTTGTAGTCCTGATAAGACGCG‑TTAGCGGCGCATCAGGCATCCGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGc                <  1:325274/275‑1 (MQ=255)                                                                                                                                                                                                                                              ggCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTTGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTCCGGATGGCGCGGGATCTTGTAGTCCTGATAAGACGCGTTAGCGCACCATCTGGCATCTGCAAACGACTGCTGGCTGc                >  1:509638/1‑274 (MQ=14)                                                                                                                                                                                                                                              ggCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGACTTATCCGGCCTCCGGTTGGCGCGTGCATTTGTAGGCCTGCTAAGACGCGTTAGCGTCGCATCAGGCAGCCGcaacaccccaccggatgc                >  2:262281/1‑258 (MQ=11)                                                                                                                                                                                                                                                   tgacggttGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATaa           <  1:383280/268‑1 (MQ=255)                                                                                                                                                                                                                                                            tAGCCCTGGTACGACGCG‑GTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCTGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGAGAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGt  <  1:163343/274‑1 (MQ=255)                                                                                                                                                                                                                                                               gCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGAt                                                                >  2:342835/1‑209 (MQ=39)                                                                                                                                                                                                                                                                   gATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGa                                                            <  1:342836/209‑1 (MQ=38)                                                                                                                                                                                                                                                                    aTAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGt                                                                         >  1:238239/1‑195 (MQ=38)                                                                                                                                                                                                                                                                    aTAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCg               >  2:383279/1‑253 (MQ=38)                                                                                                                                                                                                                                                                        gACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGc                                                                     <  2:238238/195‑1 (MQ=38)                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                 GGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGT  >  NC_000913/374833‑375356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑