Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,504,726:1 +G 8.0% coding (1010/1149 nt) insQ → IS609 transposase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,504,7241.G 8.0% 188.8 / 11.1 50coding (1008/1149 nt)insQIS609 transposase B
Reads supporting (aligned to +/- strand):  ref base . (22/24);  new base G (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

TGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAG  >  NC_000913/1504454‑1504997
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                 
tGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGat                                                                                                                                                                                                                                                                               >  2:30949/1‑275 (MQ=255)
tGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGAGTTGCGCGTACTGTGGTCGTACAGCGACAGAGAACCGCCTGTCACAAAGTAAATTCAGCAGCCCGGTATGTggca                                                                                                                                                                                                                                                                               >  1:172819/1‑273 (MQ=255)
      tttGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTAAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTGCTGTGGTAATCCAGCGAAAGAGAACCGCCTGGCACAAAGTCAATTCAGATGCCCGGCATGGGGATATACAg                                                                                                                                                                                                                                                                         >  1:410348/1‑275 (MQ=255)
            gggcAGCAAATTGGCAAAGCCAGAAGCGGGTACGGTCAGCCAGCCGGGGCGCAAGGTCCGGGCAAAATCAGGGTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCAAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACg                                                                                                                                                                                                                                                                   <  1:60866/272‑1 (MQ=255)
            ggcgcataacagggtaaacgaggcagcGGTGACGGTCGGCCAGCCTGGGGGGAAGGTCCGGGCAAAATCAGGTTTAAACCGTGCGATACTGGTTAAGGGCTGGGATGAAATGCGCCGCCAGCTGGCGTATAAGCAGCGCTGGCGTGGCGGTCAGGTGCTTGGTTTTCCGCCAGCTTATACAAGCCAGCGTGGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTTACAAAGTAAATTGAGATGCCAGGTATGTGGATATACAGCGAACg                                                                                                                                                                                                                                                                   <  2:316811/253‑1 (MQ=255)
              tcagcacatggTCAAAGTCACCGCGGGGTCCCGGCAGCCCGCATGGGCGCAAGGTCCGGGCAAAATACGGTTTAAACCGTCCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGcc                                                                                                                                                                                                                                                                 <  1:161813/265‑1 (MQ=255)
                        gTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTAAGTGGATATACAGCGAACGCCGATGTAAATg                                                                                                                                                                                                                                                       >  1:78639/1‑275 (MQ=255)
                            aaGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGgc                                                                                                                                                                                                                                                     <  1:95932/273‑1 (MQ=255)
                                 agcagcGGGTACGGTCAGCCAGCCGGGGCGCAATGGCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGc                                                                                                                                                                                                                                               <  1:30949/274‑1 (MQ=255)
                                             ggTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGATTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGCGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAgcgg                                                                                                                                                                                                                                  >  1:149649/1‑275 (MQ=255)
                                                   ccagccGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCgggg                                                                                                                                                                                                                             >  2:422180/1‑274 (MQ=255)
                                                       ccGGGGAGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGAGACTGGATCAGGGCGGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTGTACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACg                                                                                                                                                                                                                         <  1:501166/274‑1 (MQ=255)
                                                            gcgcAAGGGCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAAGGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGAGGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGtt                                                                                                                                                                                                                    <  2:149648/274‑1 (MQ=255)
                                                                                           cGTTCGATACTGGTTCAGGGCTGTTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGGTCTGGCGTGGCGGTCAGGTTCGTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGgccg                                                                                                                                                                                    <  1:181177/275‑1 (MQ=255)
                                                                                                       gATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGc                                                                                                                                                                         >  1:377935/1‑275 (MQ=255)
                                                                                                       gATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGgcg                                                                                                                                                                                                                                                    >  1:177737/1‑199 (MQ=255)
                                                                                                           aGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCg                                                                                                                                                                                                                                                <  2:177736/199‑1 (MQ=255)
                                                                                                            gggCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAAc                                                                                                                                                                   >  2:376968/1‑275 (MQ=255)
                                                                                                                tGGTAGTAAATGCGCCGCCAGCGTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTTCTGTTCCGCCAGCGGATACAAGCCAGCGTTGGGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCAc                                                                                                                                                               <  1:71635/275‑1 (MQ=255)
                                                                                                                  gTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATAGACAGCGAACGCCGCTGTAAATGGCGCTCGCAAACTTTTAGCGGCTGGACACGACGTTCTTACCTGTGGAGAGATCGTGCCGCCATGCAGCCCGTTGAACGAGGAAccacccg                                                                                                                                                             >  1:436598/1‑270 (MQ=255)
                                                                                                                   tATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCgggg                                                                                                                                                                                                                             >  1:240907/1‑210 (MQ=255)
                                                                                                                   tATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCCGGAACCCACCGa                                                                                                                                                            >  1:521408/1‑275 (MQ=255)
                                                                                                                       aaaTGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACg                                                                                                                                                                                                                         <  2:240906/210‑1 (MQ=255)
                                                                                                                            cgccgcCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCCCGCCGGTCTTGCCTGTTGGGAGATGGTGCAGGCAGGCCGGCCGTTTAAGCCGGAACCCACCGAAATGGTTCa                                                                                                                                                   >  2:328001/1‑275 (MQ=255)
                                                                                                                             gccgccAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGAGGGGCACGCCGTTCTTGCCTGTGGAGAGCTGGTGCAGTAAGGCCGCCCGTTTAAGGAGGAACCCACCGAAATGGTTCa                                                                                                                                                   >  2:141991/1‑274 (MQ=255)
                                                                                                                                 cctgcggtcGTATAAGCAGCTACTGGTGGGCGGTCAGGTGCTTGCGGTTCCGCCAGCGTATACAACCCAGCGTTGCGCGTACTGTGGTCATACAGGGAAAGAGAACCGCCTGTCACAAAGTAAATGCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCg                                                                                                                                               <  1:499869/266‑1 (MQ=255)
                                                                                                                                  cAGCTTGCGGATAAGCAGCTCTGGGGTGGCGGGCAGGTGCTTGCGGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCGGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGGTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGa                                                                                                                                              <  2:172818/274‑1 (MQ=255)
                                                                                                                                       tGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGcc                                                                                                                                         <  2:377934/275‑1 (MQ=255)
                                                                                                                                         cGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTTCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGCAATGATTCAGGGGACAGCCTg                                                                                                                                       >  1:91280/1‑274 (MQ=255)
                                                                                                                                         cGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGGTGACGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTg                                                                                                                                       >  1:326628/1‑274 (MQ=255)
                                                                                                                                                 ccgcTGTGGCGTGGCGGGCAGGTGCTTGCTGTTCCGCCAGCGTAGACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAGCCGCCTGTCACAAAGTAAATGCAGATGCCAGGTATGTGGGTATACAGCGAACGCCGATGTAAATGGCGCTCGTAACATTTTAGCGGCGTGGCTCGCCGTTTGTGCCTGGGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCa                                                                                                                              <  2:238218/273‑1 (MQ=255)
                                                                                                                                                               gcgGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATgg                                                                                                                                                                                                                                                      >  1:465398/1‑142 (MQ=255)
                                                                                                                                                                  agcatggtcggccacgcccGCCAACTGATACAAGCCTGCGTTCGGCGGACTGTGGTACATCACGGAAAGAGAGCCGCCTGTCACAAAGTAAATTCAGATGGCAGGTATGTGAATATACAGCGAACTCCGATGGAAATGTCGCTCGCGACAGTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGGGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATGCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTc                                                                                                              <  2:410347/258‑1 (MQ=255)
                                                                                                                                                                   tCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCt                                                                                                                                                                                                                                                  <  2:465397/142‑1 (MQ=255)
                                                                                                                                                                   tCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTc                                                                                                              <  1:422181/274‑1 (MQ=255)
                                                                                                                                                                                  tCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGt                                                                                              <  1:376969/275‑1 (MQ=255)
                                                                                                                                                                                         gCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGAGCCACGTCCTTCAGGGCGTGGGGGAGGGAAatat                                                                                        >  2:502610/1‑275 (MQ=255)
                                                                                                                                                                                           gTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATCTTAGCGGCGGGGCACGCCGTTTTTGCCTGTGGAAAGATGGTGCCGTCATGCCGCCCGTTCAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATACACGTCCCTCAGGGCGTGGAGGAAGGCaacaac                                                                                       >  2:340810/1‑269 (MQ=255)
                                                                                                                                                                                                      aGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGAGGGTGCCGTCAGGCCGCCCGCTGAACCCAGAACCCACCGAAAGAATTCATGCGACTCACCGAAAGTAGAGAGGGTCCCAGTGCTTTGGGGCGTGGGGGGTGCCAAAATCgactcagtcccgg                                                                          >  2:205690/1‑263 (MQ=255)
                                                                                                                                                                                                       gCGGTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTc                                                                                                                                                    <  2:352172/200‑1 (MQ=255)
                                                                                                                                                                                                        cGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAAAGATGGTGCAGTCAGGACGCCCGCTGAAGCCGGAACCCACCGAAATGATTCAGGGGACAGCCAGAACCTAGCAGGGATCACCGTACTTCAGGGTGTGGGGGATGTCAATTTCGGGTTCTGTCGGCg                                                                         >  2:211345/1‑274 (MQ=255)
                                                                                                                                                                                                           tGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACGTTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGAtt                                                                      <  1:328002/274‑1 (MQ=255)
                                                                                                                                                                                                           tGCGCGTACGGTGGTCATACAGCGAAAGAGAACCGCCGGTCACAAAGTAAATTCAGTTGCCAGGTATGTGGATATACAGCGAAGGCCGATGGAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGAtt                                                                      <  2:147380/274‑1 (MQ=255)
                                                                                                                                                                                                             cgcgTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGc                                                                          >  1:400600/1‑268 (MQ=255)
                                                                                                                                                                                                             cgcgTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTaaa                                                                   <  2:521407/275‑1 (MQ=255)
                                                                                                                                                                                                                 tACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGAtt                                                                      <  2:400599/268‑1 (MQ=255)
                                                                                                                                                                                                                                    aaaGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTgg                                                                                                                                                                                                         >  2:56456/1‑119 (MQ=255)
                                                                                                                                                                                                                                        agaACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGgagag                                                                                                                                                                                                     <  1:56456/119‑1 (MQ=255)
                                                                                                                                                                                                                                            ccGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCCTAACGTAGCAGGGAACCCCGTCCTTCACGGCGGGGAGGAGGTCAATATCGGTTTCTGTCAGCGATTAAATTCAGACCCACGAAACAAACGCCTCCGCCa                                     >  2:80738/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                     tGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGCTCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCaa            >  1:26732/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                        cAGGGATGTGGGATATACAGCGAACGCCAGGGTAAATGGCGCTCGCAACAGTTTAGCGGCGGGGCACGCCGTTCTTGGCTGTGGAGGGATTGTGCAGGCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATc          <  1:502611/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                tgGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTTCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGCAAGCCTGAACGTAGCAGGGCTCCACGTCCTTCCGGGCGTGGAGGATGTCACTATCGGGTTCTGTACGCCACTTAATTCAACCACACGTAACAAACGCCACCGCCAATTCATCCCCAAACCCCAAAACCAAAccccgccac  >  2:360758/1‑268 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                 
TGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTCACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAG  >  NC_000913/1504454‑1504997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: