| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 375,079 | T→A | 10.1% | intergenic (+198/‑380) | mhpE → / → mhpT | 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 375,079 | 0 | T | A | 10.1% | 82.3 / 6.2 | 40 | intergenic (+198/‑380) | mhpE/mhpT | 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter |
| Reads supporting (aligned to +/- strand): ref base T (19/16); new base A (2/2); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-02 | |||||||||||
TTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGC > NC_000913/374823‑375342 | ttGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCTAGAACGTGCATTTTGTATGCCCATCCCAGAACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGa < 1:39902/274‑1 (MQ=255) gcCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCCTCGAATCAGGCATCTGCGCACGACTGCCGGATGGGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCCGGGGAATTTGGAGGCCTGACaaacagggtt > 1:211086/1‑266 (MQ=21) ggaggaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTCAGAATTTGTA‑GGTCTGACAAGACGCGTTAGCGTCGCATCAGGCATCTGCCCACGACTGCCTGATGCGGCGTTAACGCCTTCTCCGGCCTACGGAATGCCGC‑GGCATTTGGAGGCCGGATTAGAcgccgtag > 2:325273/1‑267 (MQ=21) ggaggaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAAGGATGGCGTGAGCATTTGGA‑GGTATGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGGACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAGGG‑ATGGCGCGGGCAGTTGTGGGGCTGATCAGAAGGG‑TTAGCGt > 1:127108/1‑275 (MQ=21) agggTATGATCGTTGACCGGGCGCTGGATCTGCGCAACAACAAATAGTGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGt < 2:502627/273‑1 (MQ=255) tGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTa > 1:502628/1‑256 (MQ=255) gcAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATACGGCCGACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TGAGAGTCGCATCAGGCATCTGCGCACGACTGCCGGATgcg > 1:178540/1‑274 (MQ=255) aacaaATAATGAGGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGa < 1:354520/275‑1 (MQ=255) aaaTAAGAATCACTGCCGACAACGTGCCTTTGCTAACCCCATCCATGCACGACTGCCAGATGCGTATTGACCGCCTTATCCCGCCTACGCATCGCGTCAAAATTTGTA‑GGTCTGTTAAGACGCGTTAGCTTCCCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAAc < 2:204023/274‑1 (MQ=14) gaggcggcgggggacggggaTTTGGTGTGCCCATCACATCGCGGCGGCCCGAGGCGGCGTGACCGCCGTGTGGGGCGTACGGATGGCGGGAGAATTGGGA‑GGGCTGATAAGACGCGTTGGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc < 2:135266/255‑1 (MQ=11) atgcCTGCCGAGAACGTGCATTTTGTATGCCCAACCCAGCACGACTGCCGGATGCGGCGTGAACGCCGTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc < 1:115485/274‑1 (MQ=255) tGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGAATACGGCATCTGCGAACGACTGCCGGAGGCGGCGTGGACGCCTTATCCGGCCt > 1:374258/1‑275 (MQ=255) aaCGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGACTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCG‑TTAGCGTAGCATCAGGAATCTGCGCACGACTGCCGGATGCGGCGTGAACGCGTTATCCGGCCTACggggt > 1:527800/1‑273 (MQ=255) tggatgtggtgtGCCCATCCCAGCACGGCGGCCGGATGCGGAGTGAGCGCCTTATCCGGCCTACGGATGGCGGGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg < 2:528819/263‑1 (MQ=255) gCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTCGCGTCGCATCAGGCAGCTTCGTCCGACGGCCGGACGCGGCGTTGACCACTTGTCCGGCCTACGGGTGgcg > 2:126201/1‑273 (MQ=14) tGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCCCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGACCGACTGCCGGATGCGGCGTGAACCCCCTCTCCGGCCTACAGGTTGCGCGGGAAtt > 2:293008/1‑275 (MQ=255) cagcccAGCACGACGGCCGGATGCGGCGTGATCGCCGTATCCGGCCGACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGc < 1:271318/271‑1 (MQ=255) tCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGAGGAGTGAACGCCTTATCAGGACTACGG‑ATGGCGCGGGAATTGGTAGGCGTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGACGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAGTGTGTAGGCCTg > 1:69618/1‑275 (MQ=255) cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGAACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGTACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGCGTGGCGCGAGAACTTGTAGGCCTGATTAGACGCGt > 1:454958/1‑275 (MQ=255) ggATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGTGAGAATTTGTG‑GGTCGGATAAGACGCGTTAGCGTGGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCg < 2:32188/275‑1 (MQ=255) gagggggatggagcgcctgtgccGGCCTACTGAGGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGt < 2:178539/254‑1 (MQ=14) cggggTGAACGCCTTATCCGGCCTACGGATGGCGGGAGAATTTTGA‑GGTCTGGTAGGACGCGTTAGGGTCGCATCAGGCACCTGCGCCCGACTGCCGGATGCGGCTTGAACGCCTTATCCGGCCTATGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCa < 1:126202/275‑1 (MQ=11) gcgTGAACGCCTTTCGCGGCCTAGGCATGGCGTG‑GCAATTGTAGGGTCTGATAAGACGCGTTAGCGTCTCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCAt < 2:69617/274‑1 (MQ=11) cgTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGCATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGAATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGGGTGGCGCGAGAGTTTGTAGGCCTGATAAGACGCGTAAGCGTCGCATcg > 1:368094/1‑274 (MQ=255) ggCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTCTCCGGCCTACGGCTGGCGCGAGACGTTTTAGGCCTGATAAGACGCCGTAGCGTCGCATCTGGCATCTGCGCTCGACTGCCGGCTgcg > 2:503621/1‑275 (MQ=14) ggCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGAGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAAGCCTGATAAGACGCGTTAGCGACGCATCAGGCATCTGCGCCCGACGGCCGGAcgcg > 1:283765/1‑275 (MQ=255) aTAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTTGGCACCACTGGCGGATTCGCAGTAAACGCCTTAGCCgccat > 2:163342/1‑271 (MQ=14) gcggcggccggcagcgggggaCGACTGGCGGCTGCGGCGGTATCGCCTTATCCGGCCTACGG‑TGGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGGACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGa < 2:368093/255‑1 (MQ=14) tCGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACAC‑TTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGt > 1:120075/1‑163 (MQ=11) cGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACAC‑TTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCgg > 1:76564/1‑230 (MQ=11) aTCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACAC‑TTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg < 2:120074/163‑1 (MQ=11) tCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACAC‑TTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATgc < 2:76563/230‑1 (MQ=11) cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATCAGACGCGTTAGCGTCGCATCCGGCATCTGCGCACGACTGCCGGCTGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAAGTTGTAGGCCTggtcaggagcgt > 1:307007/1‑264 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCGTAATAAGACGCGTTAGCGTAGCATCAGGCATCTCCGCACGACCGCCGGATTAGGCGGAAACGCCTCATTCGGCCTACTGACACCGGGGGAATTCATAGCACTGATAAGGCGCGGTa > 2:474311/1‑274 (MQ=14) tGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTGAACCGGCCTACGGGTGGCGCGAGAATTTGTCGGCCTGATAAGACGCGTTAGCGTCACGTCAGGCATCTACGCACGACTGCCGGATGCGGCGTAAGCGCCTTATCCGGACTACGGATGGAGCGGGAACTTGTAGGCCTGATAAGACGCGTTCGCGCCg > 1:525121/1‑275 (MQ=14) aaccccgtgtgcggcgggcGG‑AGGGCGGGGGAATTTGGAGGCCGGAGAAGACGAG‑TTAGAGTCGCGTCAGGCATGTGCGCACGACTGCCGGATGCGGCGTGGACGCCTGATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGc < 2:499768/256‑1 (MQ=11) ctCCGGCCTTCGG‑ATGGCGCGGTAATTTGTAGGCCTTATAAGACGCT‑TTAGCTGCGCATCAGGCATCTGCGCACGACTGCCGGATGCGTCGTGAACGCCTTATCCTGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGcaca < 1:461707/274‑1 (MQ=255) tGGCGCGGGAATTTGTAGTCCTGATAAGACGCG‑TTAGCGGCGCATCAGGCATCCGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGc < 1:325274/275‑1 (MQ=255) ggCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTTGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTCCGGATGGCGCGGGATCTTGTAGTCCTGATAAGACGCGTTAGCGCACCATCTGGCATCTGCAAACGACTGCTGGCTGc > 1:509638/1‑274 (MQ=14) ggCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGACTTATCCGGCCTCCGGTTGGCGCGTGCATTTGTAGGCCTGCTAAGACGCGTTAGCGTCGCATCAGGCAGCCGcaacaccccaccggatgc > 2:262281/1‑258 (MQ=11) | TTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGC > NC_000913/374823‑375342 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |