Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	375,081	T→C	10.1%	intergenic (+200/‑378)	mhpE → / → mhpT	4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	375,081	0	T	C	10.1%	89.4 / 4.9	40	intergenic (+200/‑378)	mhpE/mhpT	4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base T (19/16);  new base C (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-02

TTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAA  >  NC_000913/374823‑375347                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                            ttGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCTAGAACGTGCATTTTGTATGCCCATCCCAGAACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGa                                                                                                                                                                                                                                                              <  1:39902/274‑1 (MQ=255)       gcCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCCTCGAATCAGGCATCTGCGCACGACTGCCGGATGGGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCCGGGGAATTTGGAGGCCTGACaaacagggtt                                                                                                                                                                                                                                                        >  1:211086/1‑266 (MQ=21)           ggaggaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTCAGAATTTGTA‑GGTCTGACAAGACGCGTTAGCGTCGCATCAGGCATCTGCCCACGACTGCCTGATGCGGCGTTAACGCCTTCTCCGGCCTACGGAATGCCGC‑GGCATTTGGAGGCCGGATTAGAcgccgtag                                                                                                                                                                                                                                                      >  2:325273/1‑267 (MQ=21)           ggaggaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAAGGATGGCGTGAGCATTTGGA‑GGTATGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGGACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAGGG‑ATGGCGCGGGCAGTTGTGGGGCTGATCAGAAGGG‑TTAGCGt                                                                                                                                                                                                                                                  >  1:127108/1‑275 (MQ=21)             agggTATGATCGTTGACCGGGCGCTGGATCTGCGCAACAACAAATAGTGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGt                                                                                                                                                                                                                                                  <  2:502627/273‑1 (MQ=255)                          tGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTa                                                                                                                                                                                                                                                      >  1:502628/1‑256 (MQ=255)                                              gcAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATACGGCCGACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TGAGAGTCGCATCAGGCATCTGCGCACGACTGCCGGATgcg                                                                                                                                                                                                                >  1:178540/1‑274 (MQ=255)                                                   aacaaATAATGAGGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGa                                                                                                                                                                                                          <  1:354520/275‑1 (MQ=255)                                                      aaaTAAGAATCACTGCCGACAACGTGCCTTTGCTAACCCCATCCATGCACGACTGCCAGATGCGTATTGACCGCCTTATCCCGCCTACGCATCGCGTCAAAATTTGTA‑GGTCTGTTAAGACGCGTTAGCTTCCCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAAc                                                                                                                                                                                                        <  2:204023/274‑1 (MQ=14)                                                              gaggcggcgggggacggggaTTTGGTGTGCCCATCACATCGCGGCGGCCCGAGGCGGCGTGACCGCCGTGTGGGGCGTACGGATGGCGGGAGAATTGGGA‑GGGCTGATAAGACGCGTTGGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                                                                <  2:135266/255‑1 (MQ=11)                                                              atgcCTGCCGAGAACGTGCATTTTGTATGCCCAACCCAGCACGACTGCCGGATGCGGCGTGAACGCCGTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                                                                <  1:115485/274‑1 (MQ=255)                                                                   tGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGAATACGGCATCTGCGAACGACTGCCGGAGGCGGCGTGGACGCCTTATCCGGCCt                                                                                                                                                                                          >  1:374258/1‑275 (MQ=255)                                                                          aaCGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGACTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCG‑TTAGCGTAGCATCAGGAATCTGCGCACGACTGCCGGATGCGGCGTGAACGCGTTATCCGGCCTACggggt                                                                                                                                                                                   >  1:527800/1‑273 (MQ=255)                                                                              tggatgtggtgtGCCCATCCCAGCACGGCGGCCGGATGCGGAGTGAGCGCCTTATCCGGCCTACGGATGGCGGGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg                                                                                                                                                                                <  2:528819/263‑1 (MQ=255)                                                                               gCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTCGCGTCGCATCAGGCAGCTTCGTCCGACGGCCGGACGCGGCGTTGACCACTTGTCCGGCCTACGGGTGgcg                                                                                                                                                                                >  2:126201/1‑273 (MQ=14)                                                                                     tGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCCCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGACCGACTGCCGGATGCGGCGTGAACCCCCTCTCCGGCCTACAGGTTGCGCGGGAAtt                                                                                                                                                                        >  2:293008/1‑275 (MQ=255)                                                                                             cagcccAGCACGACGGCCGGATGCGGCGTGATCGCCGTATCCGGCCGACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGc                                                                                                                                                                 <  1:271318/271‑1 (MQ=255)                                                                                               tCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGAGGAGTGAACGCCTTATCAGGACTACGG‑ATGGCGCGGGAATTGGTAGGCGTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGACGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAGTGTGTAGGCCTg                                                                                                                                                              >  1:69618/1‑275 (MQ=255)                                                                                                          cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGAACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGTACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGCGTGGCGCGAGAACTTGTAGGCCTGATTAGACGCGt                                                                                                                                                   >  1:454958/1‑275 (MQ=255)                                                                                                               ggATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGTGAGAATTTGTG‑GGTCGGATAAGACGCGTTAGCGTGGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCg                                                                                                                                              <  2:32188/275‑1 (MQ=255)                                                                                                                gagggggatggagcgcctgtgccGGCCTACTGAGGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGt                                                                                                                                             <  2:178539/254‑1 (MQ=14)                                                                                                                    cggggTGAACGCCTTATCCGGCCTACGGATGGCGGGAGAATTTTGA‑GGTCTGGTAGGACGCGTTAGGGTCGCATCAGGCACCTGCGCCCGACTGCCGGATGCGGCTTGAACGCCTTATCCGGCCTATGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCa                                                                                                                                         <  1:126202/275‑1 (MQ=11)                                                                                                                      gcgTGAACGCCTTTCGCGGCCTAGGCATGGCGTG‑GCAATTGTAGGGTCTGATAAGACGCGTTAGCGTCTCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCAt                                                                                                                                        <  2:69617/274‑1 (MQ=11)                                                                                                                       cgTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGCATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGAATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGGGTGGCGCGAGAGTTTGTAGGCCTGATAAGACGCGTAAGCGTCGCATcg                                                                                                                                      >  1:368094/1‑274 (MQ=255)                                                                                                                                                  ggCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTCTCCGGCCTACGGCTGGCGCGAGACGTTTTAGGCCTGATAAGACGCCGTAGCGTCGCATCTGGCATCTGCGCTCGACTGCCGGCTgcg                                                                                                           >  2:503621/1‑275 (MQ=14)                                                                                                                                                  ggCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGAGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAAGCCTGATAAGACGCGTTAGCGACGCATCAGGCATCTGCGCCCGACGGCCGGAcgcg                                                                                                           >  1:283765/1‑275 (MQ=255)                                                                                                                                                                         aTAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTTGGCACCACTGGCGGATTCGCAGTAAACGCCTTAGCCgccat                                                                                     >  2:163342/1‑271 (MQ=14)                                                                                                                                                                                        gcggcggccggcagcgggggaCGACTGGCGGCTGCGGCGGTATCGCCTTATCCGGCCTACGG‑TGGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGGACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGa                                                                      <  2:368093/255‑1 (MQ=14)                                                                                                                                                                                         tCGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACAC‑TTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGt                                                                                                                                                                                    >  1:120075/1‑163 (MQ=11)                                                                                                                                                                                          cGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACAC‑TTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCgg                                                                                                                >  1:76564/1‑230 (MQ=11)                                                                                                                                                                                             aTCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACAC‑TTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg                                                                                                                                                                                <  2:120074/163‑1 (MQ=11)                                                                                                                                                                                              tCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACAC‑TTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATgc                                                                                                            <  2:76563/230‑1 (MQ=11)                                                                                                                                                                                                                cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATCAGACGCGTTAGCGTCGCATCCGGCATCTGCGCACGACTGCCGGCTGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAAGTTGTAGGCCTggtcaggagcgt                                              >  1:307007/1‑264 (MQ=255)                                                                                                                                                                                                                   ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCGTAATAAGACGCGTTAGCGTAGCATCAGGCATCTCCGCACGACCGCCGGATTAGGCGGAAACGCCTCATTCGGCCTACTGACACCGGGGGAATTCATAGCACTGATAAGGCGCGGTa                                            >  2:474311/1‑274 (MQ=14)                                                                                                                                                                                                                        tGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTGAACCGGCCTACGGGTGGCGCGAGAATTTGTCGGCCTGATAAGACGCGTTAGCGTCACGTCAGGCATCTACGCACGACTGCCGGATGCGGCGTAAGCGCCTTATCCGGACTACGGATGGAGCGGGAACTTGTAGGCCTGATAAGACGCGTTCGCGCCg                                      >  1:525121/1‑275 (MQ=14)                                                                                                                                                                                                                                 aaccccgtgtgcggcgggcGG‑AGGGCGGGGGAATTTGGAGGCCGGAGAAGACGAG‑TTAGAGTCGCGTCAGGCATGTGCGCACGACTGCCGGATGCGGCGTGGACGCCTGATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGc                              <  2:499768/256‑1 (MQ=11)                                                                                                                                                                                                                                         ctCCGGCCTTCGG‑ATGGCGCGGTAATTTGTAGGCCTTATAAGACGCT‑TTAGCTGCGCATCAGGCATCTGCGCACGACTGCCGGATGCGTCGTGAACGCCTTATCCTGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGcaca                     <  1:461707/274‑1 (MQ=255)                                                                                                                                                                                                                                                        tGGCGCGGGAATTTGTAGTCCTGATAAGACGCG‑TTAGCGGCGCATCAGGCATCCGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGc       <  1:325274/275‑1 (MQ=255)                                                                                                                                                                                                                                                         ggCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTTGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTCCGGATGGCGCGGGATCTTGTAGTCCTGATAAGACGCGTTAGCGCACCATCTGGCATCTGCAAACGACTGCTGGCTGc       >  1:509638/1‑274 (MQ=14)                                                                                                                                                                                                                                                         ggCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGACTTATCCGGCCTCCGGTTGGCGCGTGCATTTGTAGGCCTGCTAAGACGCGTTAGCGTCGCATCAGGCAGCCGcaacaccccaccggatgc       >  2:262281/1‑258 (MQ=11)                                                                                                                                                                                                                                                              tgacggttGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATaa  <  1:383280/268‑1 (MQ=255)                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                            TTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTA‑GGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGG‑ATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAA  >  NC_000913/374823‑375347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑