Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,909,451 A→G 7.2% F217L (TTT→CTT)  kdgR ← KDG regulon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,909,4510AG 7.2% 166.4 / 2.1 55F217L (TTT→CTT) kdgRKDG regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (20/30);  new base G (2/2);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

GATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGTTTAATATAACGCCACCTTCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTGCTGTACAGCGGATTACGACGCCCAATCCGTGAATACATGCGCAAATTGTACAT  >  NC_000913/1909181‑1909721
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                              
gATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGTTTAATATAACGCCACCTTCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGc                                                                                                                                                                                                                                                                              <  2:529409/273‑1 (MQ=255)
  ttCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGTTTAATATAACGCAACCGTCAACTTTAGTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGAGAGTCGTGATAACCCATTTGGACAGAAATTTTGCGCTCAGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGAt                                                                                                                                                                                                                                                                           <  1:39040/274‑1 (MQ=255)
                tAAAATACTCTTATAGCAAGCGCGTTTAATATAACGCCACCTTCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTa                                                                                                                                                                                                                                                               <  1:281124/272‑1 (MQ=255)
                   aaTACTCTTATAGCAAGCGCGTTTAATATAACGCCACCTTCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCaa                                                                                                                                                                                                                                                         >  1:394336/1‑275 (MQ=255)
                        tctTATAACAAGCGCGTTTAATATAACGCCACCTTCAACTTTATTTTTCTCAGTCTAAATAAAAGACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCAt                                                                                                                                                                                                                                                    <  2:347343/275‑1 (MQ=255)
                            ataGCAAGCGCGTTTAATATAACGCCACCTTCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACAACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGc                                                                                                                                                                                                                                                 >  1:405065/1‑274 (MQ=255)
                                  aGCGCGTTTAATATAACGCCACCTTCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCCCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAGGCGATCAAATACCGGTACCGCAATGCATCGCAGCCctt                                                                                                                                                                                                                                          >  2:454336/1‑275 (MQ=255)
                                    cgcgTTTAATATAACGCCACCTTCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCcttct                                                                                                                                                                                                                                        >  2:98102/1‑275 (MQ=255)
                                        tttAATATAACGCCACCTTCAACTTTATTTTTCTCATACTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTTCTCTCATCAGAACGGTTAGTCGTGATAACCCCTTTGGGCACAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGCGAAACGCAACGTCGGGAAGTAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCACATACCGGTACCGCAATGCATCGCACCCCTTCTTCCt                                                                                                                                                                                                                                    >  1:112236/1‑275 (MQ=255)
                                                 aCGCCACCTTCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAACCGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCACACGCGAACAAATACCGGTACCGCAATGCATCGCAGCCCTTCTCCCTGCTCTTCa                                                                                                                                                                                                                            >  2:359180/1‑274 (MQ=255)
                                                       ccTTCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAACTTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCACATACCGGTACCGCAATGCATCGCAGACCTTCTTCCTGCCCTTCATTATCt                                                                                                                                                                                                                      >  1:106658/1‑274 (MQ=255)
                                                       ccTTCAACTGTATTTTTCCCATGCTAAAAAAAGAACCGTTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGAAAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCtt                                                                                                                                                                                                                     <  2:508459/275‑1 (MQ=255)
                                                         ttCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCg                                                                                                                                                                                                                   <  1:140903/275‑1 (MQ=255)
                                                                              cTAAATAAAGAACCTGTCTGTTTGATAATCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCAcc                                                                                                                                                                                              <  1:400323/275‑1 (MQ=255)
                                                                                            tgtctgtTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAg                                                                                                                                                                                                                                                                               <  2:512455/180‑1 (MQ=255)
                                                                                              tctgtTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCCCCCTACCCCTCCTCGCGCCCCTGGTCCAGACCGGGt                                                                                                                                                                               >  2:161359/1‑274 (MQ=255)
                                                                                                  tttGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCCGCTCGCGCACCTGGTCCAGAACGGGtaataa                                                                                                                                                                          <  1:319457/275‑1 (MQ=255)
                                                                                                  tttGATATGCGGACAGGTTCTTACTCCCACAAGAACGGATGGTGGTGTTAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTAGGCAACATGGCGACATATTCCTGGAAACGCTCTTCAGAGAAACGCAACGTCGAGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGtaataa                                                                                                                                                                          <  2:106657/275‑1 (MQ=255)
                                                                                                       tatGCCGACAAGTTCTTACTCTCATCAGACCGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAACCGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGc                                                                                                                                                                       <  1:161360/273‑1 (MQ=255)
                                                                                                             tgaaGGTTCTTACTCACAGCAACACGGATAGTCGCGATAACCCATTTTGGCAGAAAGTTTGCGCTCTGCGGTATGCAACAGTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCGCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTAc                                                                                                                                                               <  1:266662/272‑1 (MQ=255)
                                                                                                             gACAGGTTTTTACTCTCACCAGAACGGATCGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTAGGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTAc                                                                                                                                                               <  2:478829/275‑1 (MQ=255)
                                                                                                                           cgcatcgtcaCGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGGATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGATAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATTACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGc                                                                                                                                                 <  1:209215/266‑1 (MQ=255)
                                                                                                                                 aGAACGGATAGTGTGGATAACCCATTTGGGCAGAAATTTTGCGCGGTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCt                                                                                                                                                <  2:394335/270‑1 (MQ=255)
                                                                                                                                 aGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCt                                                                                                                                                <  1:480194/270‑1 (MQ=255)
                                                                                                                                  gcaCGGATATTCGTGATAACCCATTTGGGAGGACATTTTGCGCGCTGCGTTATGCAACACTGCGACATATTCCTGTAAACGCGCTGCAGGGAAGCGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCAATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTAc                                                                                                                                          <  1:452526/273‑1 (MQ=255)
                                                                                                                                  gAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAGGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTAc                                                                                                                                          <  1:454337/275‑1 (MQ=255)
                                                                                                                                  gAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTAc                                                                                                                                          <  1:98103/275‑1 (MQ=255)
                                                                                                                                  gAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTAc                                                                                                                                          <  1:476823/275‑1 (MQ=255)
                                                                                                                                  gAACGGAGAGTCGTGACAACCCATTTGGGCAGCAATTTTGCGCGCTGCGGTATTCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTAc                                                                                                                                          <  1:201800/275‑1 (MQ=255)
                                                                                                                                        aTAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCCCGCGCACCTGGTCCCGAACGGGTAATAACGCTTCTTGCATGGTGCTGGTCCCCTCGGTAcgtggt                                                                                                                                     >  2:4530/1‑269 (MQ=255)
                                                                                                                                                 aTAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAGGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGTTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTATTGCGTTTATAccccc                                                                                                                           >  2:7831/1‑274 (MQ=255)
                                                                                                                                                     cccATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATCGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGCACTAACGCTTCTGTACTGGTGATGGTCGGCTGGGTACTGCGTTTATACTCCACGcc                                                                                                                       >  1:146157/1‑275 (MQ=255)
                                                                                                                                                                            cggtGCGGTATGCAACATTGAGAAATAGTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACTCCAAAGCGATCAAATACCGGTACCGCAATGCAGCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCat                                                                                                <  2:104647/272‑1 (MQ=255)
                                                                                                                                                                                              tGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTc                                                                                                                                                                                         >  2:202907/1‑168 (MQ=255)
                                                                                                                                                                                                  aaatatGCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGa                                                                                                                                                                                     <  1:202908/166‑1 (MQ=255)
                                                                                                                                                                                                     tatTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTAc                                                                       >  2:86178/1‑275 (MQ=255)
                                                                                                                                                                                                                     ctTCAGAGAAACGGAACGTCGTGACAGAGATGCTCAAACCGGCAAGGACCACGCCAAAGCAATCAATTACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTg                                                       <  1:300573/275‑1 (MQ=255)
                                                                                                                                                                                                                                cGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTcc                                                                                                                                                                                                                                     >  2:92392/1‑90 (MQ=255)
                                                                                                                                                                                                                                cGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACt                                                                                                                                         >  1:73631/1‑182 (MQ=255)
                                                                                                                                                                                                                                cGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTCATGGTCCGCTCGGTACTGCGGTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGAGGTGTTGTACAGAgg                                            >  2:166285/1‑275 (MQ=255)
                                                                                                                                                                                                                                cGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGAAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTCCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTCCTGGTGATGGTCCGCTCGGTCCTGCGTTTATACCCCACGCCCCCAAGAATTTCATTCACTTCATCCCGATCGCGCCATGCCAGCAGTACCTTACTCATCGCGGTGCTGTaacccga                                            >  2:506055/1‑269 (MQ=255)
                                                                                                                                                                                                                                cGCAACGTCGGGAAGGAGATGCTAAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCGTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCCTCGCGATCGCGCCAAGCCCGCAATAACTTTCCAAACGCggatcttgcacgagg                                            >  1:153187/1‑262 (MQ=255)
                                                                                                                                                                                                                                    aCGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGct                                                                                                                                                                                                                                 <  1:92393/90‑1 (MQ=255)
                                                                                                                                                                                                                                    aCGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGt                                                                                                                                     <  2:73630/182‑1 (MQ=255)
                                                                                                                                                                                                                                     cGTCGGGAATGAGATGCTCAACCCGGCAATGACCACGCCAAGGCGTTCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTGCTGTACAGCGGATTac                                       <  1:7831/275‑1 (MQ=255)
                                                                                                                                                                                                                                     cGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTTCTGTACAGCGGATTac                                       >  1:303058/1‑275 (MQ=255)
                                                                                                                                                                                                                                           gAAGGAGAGCCACAAACCGCCAATGACAACGCCAAAGCGTTCAAATACCGGTCCCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTGCTGTACAGCGGATTACGACGcc                                 <  2:146156/275‑1 (MQ=255)
                                                                                                                                                                                                                                              ggAGATTCTCAAACCTGCAATGACAACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCCGCCCTTCTTCCTGCTCTTCATTAGCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCTGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTGCTGTACAGCGGATTACGACGCCCaa                              <  1:477295/275‑1 (MQ=255)
                                                                                                                                                                                                                                               gagaTGCTCAAACCGGCAACGACCACGCCAAAGCGTTCAAATACCGGTACCCAAATGCATCGCATGCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTGCTGTACAGCGGATTACGACGCCCAAt                             <  2:405064/275‑1 (MQ=255)
                                                                                                                                                                                                                                                     cTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGAGGTTCTGAACAGCGGAATACCAAGCCCAATCCATgc                       >  1:486548/1‑274 (MQ=255)
                                                                                                                                                                                                                                                     cTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGT‑ATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCCAGGTGCTGTACAGCGGCTTACCACGCCCAAcccctcaa                      >  2:109454/1‑270 (MQ=255)
                                                                                                                                                                                                                                                               cAATGACCACGCCAAAGCTATCCATGACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGT‑ATAACGCTTCTGTACTGGTGATGTTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTGCTGTACAGCGGATTACGACGCCCAATCCGTGAATACATgcgc             <  1:109455/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTGCTATACCGCGGATTACGACGCCCCATCCGTGAATAAATGCGCAAATTGTaa    >  2:239996/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                         gCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTGCTGTACAGCGGATTACGACGCCCAATCCGTGACTACATGCGCAAATTGTCCa   >  1:85742/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                          ccAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTGCTGTACAGCGGATTACGACGCCCAATCCGTGAATACATGCGCAAATTGTACAt  <  1:86179/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                              
GATTCTCACTCCTGAGTAAAATACTCTTATAGCAAGCGCGTTTAATATAACGCCACCTTCAACTTTATTTTTCTCATGCTAAATAAAGAACCTGTCTGTTTGATATTCCGACAGGTTCTTACTCTCATCAGAACGGATAGTCGTGATAACCCATTTGGGCAGAAATTTTGCGCGCTGCGGTATGCAACATTGCGACATATTCCTGTAAACGCTCTTCAGAGAAACGCAACGTCGGGAAGGAGATGCTCAAACCGGCAATGACCACGCCAAAGCGATCAAATACCGGTACCGCAATGCATCGCAGCCCTTCTTCCTGCTCTTCATTATCTTCGCCATACCCCTGCTCGCGCACCTGGTCCAGAACGGGTAATAACGCTTCTGTACTGGTGATGGTCCGCTCGGTACTGCGTTTATACTCCACGCCCTCAAGAATTTGCTTCACTTCATCGCGATCGCGCCATGCCAGCAGTACCTTACCAATCGCGGTGCTGTACAGCGGATTACGACGCCCAATCCGTGAATACATGCGCAAATTGTACAT  >  NC_000913/1909181‑1909721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑