Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,992,437 T→G 19.3% L429* (TTA→TGA)  cyaA → adenylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,992,4370TG19.3% 137.8 / 27.5 66L429* (TTA→TGA) cyaAadenylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (23/30);  new base G (7/6);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAACGCGCCTGCGTAGGCTGGCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCGCACCCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGT  >  NC_000913/3992168‑3992709
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                
tGAACGCGCCTGCGTAGGCTGGCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAg                                                                                                                                                                                                                                                                             >  1:312895/1‑275 (MQ=255)
           gCGTAGGCGGGCGTGGCGCAGTGTTGAGCCAGTTAGTGGGGGAGGGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGGGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGAc                                                                                                                                                                                                                                                                   <  2:116768/274‑1 (MQ=255)
                     gCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTCCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCTGCGTGCTTACGCGTCAGCTGTATGCCGCGTTTGACGCATTACCAGGTAAAGTGACGCTgcgaaa                                                                                                                                                                                                                                                          >  1:126252/1‑268 (MQ=255)
                     gCGTCGCGCAGTGGTGAGCCAGTTAGTGAGCGAGTGGGTTTGGGACGAGGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAAc                                                                                                                                                                                                                                                         <  2:425259/274‑1 (MQ=255)
                      cGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATCACCTTAGCGTCTCCGCCAGTCCGCCGGCTATCGGCGTCCTGACGCGTAAGCTGTCTGCCCCGTTTGAACCCTTACCAGGTAACGTGACGCTGGTACAcc                                                                                                                                                                                                                                                        >  2:460939/1‑274 (MQ=255)
                      cGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAAcc                                                                                                                                                                                                                                                        >  1:192777/1‑274 (MQ=255)
                      cGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATAGCGGCGTGCTGACGCGTAAGATGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTAAAacc                                                                                                                                                                                                                                                       >  1:288809/1‑275 (MQ=255)
                       gTCGGGCAGTGTTGAGCCAGTTAGTGAGCGAGGGGGGTTGGAAGGAAGCTGGGTTGGCAAGGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCg                                                                                                                                                                                                                                                      <  2:312894/275‑1 (MQ=255)
                                     aGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGAGTAAGCTGTATGCCGCGTTTGAAGCATTACTCGGTGAAGTGACGCTGGTAAACCCACAGATTTCACCCGa                                                                                                                                                                                                                                        >  1:475489/1‑275 (MQ=255)
                                          gTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTCAACCCGCAGATTTCCCCCCAtccct                                                                                                                                                                                                                                   >  2:384056/1‑275 (MQ=255)
                                            tAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGAtctctc                                                                                                                                                                                                                                  <  1:394801/274‑1 (MQ=255)
                                                   cGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGa                                                                                                                                                                                                                          >  2:32136/1‑275 (MQ=255)
                                                     aGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGGTCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCCCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGTTCTCTCGGAACCGAAt                                                                                                                                                                                                                        >  1:158143/1‑275 (MQ=255)
                                                     aGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAAt                                                                                                                                                                                                                        >  2:36520/1‑275 (MQ=255)
                                                     aGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCCCAGATTTCACCCGactcccaggaacgcaac                                                                                                                                                                                                                        >  2:391448/1‑259 (MQ=255)
                                                          gtttGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGAc                                                                                                                                                                                                                   <  1:29723/273‑1 (MQ=255)
                                                           gTTGGGACGAAGCTCGTCTGGCAATGCTCGATAGCCGCGTGAACTGGAAGATTGATCAGGGGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAAACTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGAc                                                                                                                                                                                                                   <  1:460940/274‑1 (MQ=255)
                                                                           gcTGGCAAGGCTCTATAACCCCGCTAACTGGAAGATGGATCAGGTGCGTTAGGCTCAAAACGAGTTGCTCGACGCGATGCTGCAGAGCTACCGTAATCTTATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTgccg                                                                                                                                                                                                   <  1:391449/273‑1 (MQ=255)
                                                                               gCAATGCTCGATAACCGCGCTGACTGGAAGATTGATCCGGTGCGTGAGGGGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCTTCTCCGCCAGTCCTCAGGATATCGGCGTGCTGACGCTTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgg                                                                                                                                                                                               <  1:204604/274‑1 (MQ=255)
                                                                                        gATAACCGCGCAAACTGGAAGAATGATCAGGTGCGGGAGGCGAAGACCGGATTCCACTACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGTATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTaa                                                                                                                                                                                     <  1:146215/275‑1 (MQ=255)
                                                                                         aTAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTaa                                                                                                                                                                                     <  1:32136/274‑1 (MQ=255)
                                                                                              cgggttAACTGGGAGATTGCGCCGGTGCCTGAGGCGCACAACGCGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGtt                                                                                                                                                                                <  1:179523/269‑1 (MQ=255)
                                                                                               gcgcTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAtcacccgatctctcggaaccgaatctgacctttatttatgtgccgccgggccgggctaaccgttcaggt                                                                                                                                                                               <  2:192776‑M1/274‑70 (MQ=255)
                                                                                                  cTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTTACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGCCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGt                                                                                                                                                                           >  2:313023/1‑275 (MQ=255)
                                                                                                       tGGAGGATTGATGAGTTGCGGGGGGCGGACAACGAGTGGCTGCACGCGATGATGCAGGGCGACCGGACGCTTAGCCGCTGTCGGCGTCGCAATAACCTTAGCGTCTCCGCCCGTCCACAGGATATCGGCGTGCTGACGCTTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGGGAGGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                                                                                                                       <  2:513297/274‑1 (MQ=255)
                                                                                                        ggAAGTTGGATCAGGGGCGTGAGGCGCACAACGGGTTGCTCGACGCGATGATGCAGCGCTACCGTAAGCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                                                                                                                       <  2:288808/273‑1 (MQ=255)
                                                                                                        ggAAGATGGGGCAGGTGCGGGAGGCGCACAACGAGTTGCTCGACGCGAGGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTAt                                                                                                                                                                     <  2:158142/275‑1 (MQ=255)
                                                                                                                 aTCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGAt                                                                                                                                                                                                                                       >  1:31010/1‑200 (MQ=255)
                                                                                                                  tCAGGTGCGTGAGGCGAACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAtcacccgatctctcggaaccgaatctgacctttatttatgtgccgccgggccgggctaaccgttcaggttggtatctgtataaccgcgc                                                                                                                                                           <  2:507497‑M1/275‑90 (MQ=255)
                                                                                                                     ggTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGAtctct                                                                                                                                                                                                                                   <  2:31010/200‑1 (MQ=255)
                                                                                                                       gtcGTGAGGCCCACAACGAGTTGCTCGACGGGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcgcgc                                                                                                                                                       <  2:465261/272‑1 (MQ=255)
                                                                                                                        gCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGAAAGCTGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGCTCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCGAACCGTTCAGGTTGGTATCTGGATAACcggcagc                                                                                                                                                     >  1:110991/1‑270 (MQ=255)
                                                                                                                                                   cgcgATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAGGTGACGCTGGTAAACCCGCAtcacccgatctctcggaaccgaatctgacctttatttatgtgccgccgggccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagcc                                                                                                                          >  2:414701‑M1/1‑153 (MQ=255)
                                                                                                                                                   cgcgATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCCACTATTGAATCGATcatcagccacc                                                                                                                          >  2:139003/1‑273 (MQ=255)
                                                                                                                                                         agtgcaggtcgaCCGTAACTTGGCCCGCGTTGGGCGTCGCAATAACCTTAGCGTCCCCGCCAGTCCGCAGGCTATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGc                                                                                                                    <  2:9033/264‑1 (MQ=255)
                                                                                                                                                          atgCAGCGCTACCGTAATCTGTGCAGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGc                                                                                                                    <  1:382936/274‑1 (MQ=255)
                                                                                                                                                          atgCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGc                                                                                                                    <  1:139004/274‑1 (MQ=255)
                                                                                                                                                                     ccGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGa                                                                                                                                                                                                                          >  1:295300/1‑161 (MQ=255)
                                                                                                                                                                      cGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGCTCATCAGCCATCAGCCGCTGGAATATAAc                                                                                                         >  1:459751/1‑273 (MQ=255)
                                                                                                                                                                         aaTCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCt                                                                                                                                                                                                                      <  2:295299/161‑1 (MQ=255)
                                                                                                                                                                          agccgATCCGCTTTGCGCGTCGCGATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGGGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTAc                                                                                                   <  1:516885/271‑1 (MQ=255)
                                                                                                                                                                              gATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTAc                                                                                                                                                                                                                                                                                <  1:149131/98‑1 (MQ=255)
                                                                                                                                                                                   gCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCGGACGCGTAAGCTGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTGCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGATAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATaaa                                                                                          <  1:7880/275‑1 (MQ=255)
                                                                                                                                                                                    cTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAAc                                                                                         >  1:475046/1‑275 (MQ=255)
                                                                                                                                                                                       tGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAAACGCGCGCCAAATAATGAGTCGATCATCAACCATCAGCCGCTGGAATCAAACCTCTTCCTGAATAAAAtgg                                                                                      >  2:331993/1‑275 (MQ=255)
                                                                                                                                                                                              cGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTCACCGTTCAGGTTCGTATCTGTATAACCGCGCGCCCAATCTTGAGTCGAACATCCGCCCTTAACAGCTGGAATATAACCGTTACCTGAATAAACTGATTGCGTg                                                                               >  1:203582/1‑275 (MQ=255)
                                                                                                                                                                                                         tAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTaaa                                                                                                                                                                                                                                                          >  2:510921/1‑93 (MQ=255)
                                                                                                                                                                                                            cGTCTCCACCTGGCCGCAGGATAGCGGCGGGCTGACGCGTAAGCTGTATCCCGCGTTTGAACCATTACCAGGTACAGTGACGCTGGTAAACCCGCAGATTTCACCCGTGCTCCCGGAACCGAATCTGACCTTTATTTTTGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAAc                                                                  <  1:165951/274‑1 (MQ=255)
                                                                                                                                                                                                             gTCTCCGCCAGTCCGCAGGATATCGTCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCg                                                                                                                                                                                                                                                      <  1:510922/93‑1 (MQ=255)
                                                                                                                                                                                                             gTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGGAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACgg                                                                <  1:36520/275‑1 (MQ=255)
                                                                                                                                                                                                                tcCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATc                                                                                                                                                                                                                       >  1:455083/1‑121 (MQ=255)
                                                                                                                                                                                                                tcCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGc                                                                                                                    >  2:350906/1‑220 (MQ=255)
                                                                                                                                                                                                                tcCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGc                                                                                                                    <  1:350907/220‑1 (MQ=255)
                                                                                                                                                                                                                cccGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGCATCTGACCTTTATTTATGTGCTGCCGGGCCGGTCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCCAATATTGCGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTAACTGAATAAACTGGTGGCGTGTGCATGGTTTAAAGGcc                                                              >  1:27694/2‑274 (MQ=255)
                                                                                                                                                                                                                    ccAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGAc                                                                                                                                                                                                                   <  2:455082/121‑1 (MQ=255)
                                                                                                                                                                                                                             aGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAGGTGACGCTGGTAAACCCGCAtcacccgatctctcggaaccgaatctgacctttatttatgtgccgccgggccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatggtttaacggcctgctgacctcgcgcacc                                                 <  1:414702‑M1/274‑196 (MQ=255)
                                                                                                                                                                                                                                  atCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCGCACCCg                                           >  1:149447/1‑275 (MQ=255)
                                                                                                                                                                                                                                      gCGTGTTGAGGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGTTAAACCCGCATATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCGCACCCGTTTg                                       <  2:123535/275‑1 (MQ=255)
                                                                                                                                                                                                                                       ggggctgagcggTAAGCTGGATGCCGCGTTTGAAGAATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGGGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCGCACCCGTTTg                                       <  2:459750/263‑1 (MQ=255)
                                                                                                                                                                                                                                         tGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAtcacccgatctctcggaaccgaatctgacctttatttatgtgccgccgggccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacc                                                                                                      >  1:138711‑M1/1‑67 (MQ=255)
                                                                                                                                                                                                                                             gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAtcacccgatctctcggaaccgaatctgacctttatttatgtgccgccgggccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaa                                                                                                  <  2:138710‑M1/209‑147 (MQ=255)
                                                                                                                                                                                                                                                  gTAAGCTTTATGCCGCGTTTGAAGCCTTACCAGGGAAAGTGACGCTGGTAACGCCGCAGAGTCCTCCCGATCGCTCGGAACCGAATCTGACCTGTATTGAGGTGCCGCCGGGCCGGGCTAACCTTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCGCACCCGTTTGTATATTAAAGGt                           <  1:487058/275‑1 (MQ=255)
                                                                                                                                                                                                                                                          tATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCCAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTCTAACAGCCTGCTGACCTCGCGCACCCGTTTGTATATTAAAGGTAACGGCAt                   >  1:462133/1‑275 (MQ=255)
                                                                                                                                                                                                                                                            tGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAtcacccgatctctcggaaccgaatctgacctttatttatgtgccgccgggccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacatgaataacctggtggagagggcagggtttaacggcctcctgaccgtgagaacccgtctgtctattaaaggtaacggccttgccga                 >  2:482816‑M1/1‑48 (MQ=255)
                                                                                                                                                                                                                                                                 tgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTCAAGGCCTGCTGACCTCGCGCAACCGGTTCTATATTAAAGGTCACGGCATTGTCGAt            >  1:44026/2‑275 (MQ=255)
                                                                                                                                                                                                                                                                      gAAGCATGACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCGCACCCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGcc       <  2:475045/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                            ttACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCACATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATTTAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGACTGGTGACCTCGCGCCCCCGTTTGTATATTAAAGGTACGGTCATTGTCGATTTGCCTAAGt  >  1:227660/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                
TGAACGCGCCTGCGTAGGCTGGCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGTTGCTCGACGCGATGATGCAGAGCTACCGTAATCTGATCCGCTTTGCGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCGCACCCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGT  >  NC_000913/3992168‑3992709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: