Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 375,020 T→G 9.2% intergenic (+139/‑439) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913375,0200TG 9.2% 106.9 / 6.7 44intergenic (+139/‑439)mhpE/mhpT4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base T (21/19);  new base G (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-01

CACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT  >  NC_000913/374747‑375264
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      
tgcTGTGAAACGGCGGGGGCTCGTGATGGGTTAAGTGCGTTGGATATTGTCGTGGAGCGGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTGGACGTGGCGCTGGAGCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAGTTTGTATGCCCATCCCAGCACGACTGCCTGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTg                                                                                                                                                                                                                                                       <  1:509542/273‑1 (MQ=37)
cACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCCCGAATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGGCTTGCGTGCGAATTTGTAGGTTCGATAAGACGCGTTAGCGTCGCATCAGGCATCTg                                                                                                                                                                                                                                                       >  1:528820/1‑275 (MQ=255)
                                aaGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCACGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                                                                                                       >  2:214581/1‑275 (MQ=255)
                                    gtggttgttatTGTCGTTGAGCGGGGGAAACGCCGGATGGTTGGCGGCAAGGAGGATGTGATCGTGGACGTGGCGCTGGATCTGCGCAACAACACATAATGATGACTGCCGCGAACGTGCATGTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                                                                                    <  2:267215/266‑1 (MQ=21)
                                    gCGGTGGATATTTGCGTTGGGGTGGGCAAACGCCGGATGTGTGGCGGCCAGGAGGATCTGATCGTTGAGGTGGCGGTGGATCTGCGAAACAACAAATAAGGATGACTGCCGGGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCGACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                                                                                    <  2:528334/274‑1 (MQ=21)
                                    gCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                                                                                    <  1:438202/274‑1 (MQ=255)
                                       gTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGACAGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTCGGGCTGATAAGACGCGTTAGAGTCCAATCAGGACTCTGCGCACGACTGCGGGATGCGGCGGGAACGCCtacacccgc                                                                                                                                                                                                                >  2:271317/1‑267 (MQ=255)
                                       gTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATACCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATTCGGCATCTGCGCACGACTGCCGGAAGCGGCGTGAACGCCTTATCCGGc                                                                                                                                                                                                                >  1:5260/1‑275 (MQ=255)
                                              ttCGCGTTGAGCTGGGCAAACGCGGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCTCTGGATCTTCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATGTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGa                                                                                                                                                                                                        <  2:5260/275‑1 (MQ=255)
                                                    ttGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGAATCTGCGAACGACTGCCGGATGGGGCGTGAACGCCTTATACGGCATACGGATggag                                                                                                                                                                                                   >  1:204024/1‑272 (MQ=255)
                                                      gAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTgc                                                                                                                                                                                                                                                      <  1:412702/222‑1 (MQ=255)
                                                                            ttGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCTAGAACGTGCATTTTGTATGCCCATCCCAGAACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGa                                                                                                                                                                           <  1:39902/274‑1 (MQ=255)
                                                                                  gcCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCCTCGAATCAGGCATCTGCGCACGACTGCCGGATGGGGCGTGAACGCCTTATCCGGCCTACGGATGGCCGGGGAATTTGGAGGCCTGACaaacagggtt                                                                                                                                                                     >  1:211086/1‑266 (MQ=21)
                                                                                      ggaggaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTCAGAATTTGTAGGTCTGACAAGACGCGTTAGCGTCGCATCAGGCATCTGCCCACGACTGCCTGATGCGGCGTTAACGCCTTCTCCGGCCTACGGAATGCCGCGGCATTTGGAGGCCGGATTAGAcgccgtag                                                                                                                                                                   >  2:325273/1‑267 (MQ=21)
                                                                                      ggaggaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAAGGATGGCGTGAGCATTTGGAGGTATGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGGACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAGGGATGGCGCGGGCAGTTGTGGGGCTGATCAGAAGGGTTAGCGt                                                                                                                                                               >  1:127108/1‑275 (MQ=21)
                                                                                        agggTATGATCGTTGACCGGGCGCTGGATCTGCGCAACAACAAATAGTGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGt                                                                                                                                                               <  2:502627/273‑1 (MQ=255)
                                                                                                     tGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTa                                                                                                                                                                   >  1:502628/1‑256 (MQ=255)
                                                                                                                         gcAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATACGGCCGACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTGAGAGTCGCATCAGGCATCTGCGCACGACTGCCGGATgcg                                                                                                                             >  1:178540/1‑274 (MQ=255)
                                                                                                                             caacaaATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGgcgc                                                                                                                                                                                                  >  1:444646/1‑202 (MQ=255)
                                                                                                                              aacaaATAATGAGGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGa                                                                                                                       <  1:354520/275‑1 (MQ=255)
                                                                                                                                 aaaTAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGa                                                                                                                                                                                              <  2:444645/202‑1 (MQ=255)
                                                                                                                                 aaaTAAGAATCACTGCCGACAACGTGCCTTTGCTAACCCCATCCATGCACGACTGCCAGATGCGTATTGACCGCCTTATCCCGCCTACGCATCGCGTCAAAATTTGTAGGTCTGTTAAGACGCGTTAGCTTCCCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAAc                                                                                                                     <  2:204023/274‑1 (MQ=14)
                                                                                                                                         gaggcggcgggggacggggaTTTGGTGTGCCCATCACATCGCGGCGGCCCGAGGCGGCGTGACCGCCGTGTGGGGCGTACGGATGGCGGGAGAATTGGGAGGGCTGATAAGACGCGTTGGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                             <  2:135266/255‑1 (MQ=11)
                                                                                                                                         atgcCTGCCGAGAACGTGCATTTTGTATGCCCAACCCAGCACGACTGCCGGATGCGGCGTGAACGCCGTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                             <  1:115485/274‑1 (MQ=255)
                                                                                                                                              tGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGAATACGGCATCTGCGAACGACTGCCGGAGGCGGCGTGGACGCCTTATCCGGCCt                                                                                                       >  1:374258/1‑275 (MQ=255)
                                                                                                                                                     aaCGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGACTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCGTTAGCGTAGCATCAGGAATCTGCGCACGACTGCCGGATGCGGCGTGAACGCGTTATCCGGCCTACggggt                                                                                                >  1:527800/1‑273 (MQ=255)
                                                                                                                                                         tggatgtggtgtGCCCATCCCAGCACGGCGGCCGGATGCGGAGTGAGCGCCTTATCCGGCCTACGGATGGCGGGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg                                                                                             <  2:528819/263‑1 (MQ=255)
                                                                                                                                                          gCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTCGCGTCGCATCAGGCAGCTTCGTCCGACGGCCGGACGCGGCGTTGACCACTTGTCCGGCCTACGGGTGgcg                                                                                             >  2:126201/1‑273 (MQ=14)
                                                                                                                                                                tGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCCCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGACCGACTGCCGGATGCGGCGTGAACCCCCTCTCCGGCCTACAGGTTGCGCGGGAAtt                                                                                     >  2:293008/1‑275 (MQ=255)
                                                                                                                                                                        cagcccAGCACGACGGCCGGATGCGGCGTGATCGCCGTATCCGGCCGACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGc                                                                              <  1:271318/271‑1 (MQ=255)
                                                                                                                                                                          tCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGAGGAGTGAACGCCTTATCAGGACTACGGATGGCGCGGGAATTGGTAGGCGTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGACGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAGTGTGTAGGCCTg                                                                           >  1:69618/1‑275 (MQ=255)
                                                                                                                                                                                     cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGAACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGTACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGCGTGGCGCGAGAACTTGTAGGCCTGATTAGACGCGt                                                                >  1:454958/1‑275 (MQ=255)
                                                                                                                                                                                          ggATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGTGAGAATTTGTGGGTCGGATAAGACGCGTTAGCGTGGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCg                                                           <  2:32188/275‑1 (MQ=255)
                                                                                                                                                                                           gagggggatggagcgcctgtgccGGCCTACTGAGGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGt                                                          <  2:178539/254‑1 (MQ=14)
                                                                                                                                                                                               cggggTGAACGCCTTATCCGGCCTACGGATGGCGGGAGAATTTTGAGGTCTGGTAGGACGCGTTAGGGTCGCATCAGGCACCTGCGCCCGACTGCCGGATGCGGCTTGAACGCCTTATCCGGCCTATGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCa                                                      <  1:126202/275‑1 (MQ=11)
                                                                                                                                                                                                 gcgTGAACGCCTTTCGCGGCCTAGGCATGGCGTGGCAATTGTAGGGTCTGATAAGACGCGTTAGCGTCTCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCAt                                                     <  2:69617/274‑1 (MQ=11)
                                                                                                                                                                                                  cgTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGCATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGAATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGGGTGGCGCGAGAGTTTGTAGGCCTGATAAGACGCGTAAGCGTCGCATcg                                                   >  1:368094/1‑274 (MQ=255)
                                                                                                                                                                                                                             ggCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTCTCCGGCCTACGGCTGGCGCGAGACGTTTTAGGCCTGATAAGACGCCGTAGCGTCGCATCTGGCATCTGCGCTCGACTGCCGGCTgcg                        >  2:503621/1‑275 (MQ=14)
                                                                                                                                                                                                                             ggCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGAGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAAGCCTGATAAGACGCGTTAGCGACGCATCAGGCATCTGCGCCCGACGGCCGGAcgcg                        >  1:283765/1‑275 (MQ=255)
                                                                                                                                                                                                                                                    aTAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTTGGCACCACTGGCGGATTCGCAGTAAACGCCTTAGCCgccat  >  2:163342/1‑271 (MQ=14)
                                                                                                                                                                                                                                                                    tCGCATCAGGCATGGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGCCCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGt                                                                                                 >  1:120075/1‑163 (MQ=11)
                                                                                                                                                                                                                                                                     cGCATCAGGCATGGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGCCCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCgg                             >  1:76564/1‑230 (MQ=11)
                                                                                                                                                                                                                                                                        aTCAGGCATGGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGCCCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg                                                                                             <  2:120074/163‑1 (MQ=11)
                                                                                                                                                                                                                                                                         tCAGGCATGGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGCCCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATgc                         <  2:76563/230‑1 (MQ=11)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      
CACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT  >  NC_000913/374747‑375264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: