Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 375,069 G→C 10.1% intergenic (+188/‑390) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913375,0690GC10.1% 93.9 / 8.3 40intergenic (+188/‑390)mhpE/mhpT4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base G (19/17);  new base C (2/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-02

TTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGC  >  NC_000913/374799‑375342
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                  
ttGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGAATCTGCGAACGACTGCCGGATGGGGCGTGAACGCCTTATACGGCATACGGATggag                                                                                                                                                                                                                                                                                 >  1:204024/1‑272 (MQ=255)
                        ttGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCTAGAACGTGCATTTTGTATGCCCATCCCAGAACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGa                                                                                                                                                                                                                                                         <  1:39902/274‑1 (MQ=255)
                              gcCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCCTCGAATCAGGCATCTGCGCACGACTGCCGGATGGGGCGTGAACGCCTTATCCGGCCTACGGATGGCCGGGGAATTTGGAGGCCTGACaaacagggtt                                                                                                                                                                                                                                                   >  1:211086/1‑266 (MQ=21)
                                  ggaggaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTCAGAATTTGTAGGTCTGACAAGACGCGTTAGCGTCGCATCAGGCATCTGCCCACGACTGCCTGATGCGGCGTTAACGCCTTCTCCGGCCTACGGAATGCCGCGGCATTTGGAGGCCGGATTAGAcgccgtag                                                                                                                                                                                                                                                 >  2:325273/1‑267 (MQ=21)
                                  ggaggaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAAGGATGGCGTGAGCATTTGGAGGTATGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGGACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAGGGATGGCGCGGGCAGTTGTGGGGCTGATCAGAAGGGTTAGCGt                                                                                                                                                                                                                                             >  1:127108/1‑275 (MQ=21)
                                    agggTATGATCGTTGACCGGGCGCTGGATCTGCGCAACAACAAATAGTGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGt                                                                                                                                                                                                                                             <  2:502627/273‑1 (MQ=255)
                                                 tGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTa                                                                                                                                                                                                                                                 >  1:502628/1‑256 (MQ=255)
                                                                     gcAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATACGGCCGACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTGAGAGTCGCATCAGGCATCTGCGCACGACTGCCGGATgcg                                                                                                                                                                                                           >  1:178540/1‑274 (MQ=255)
                                                                         caacaaATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGgcgc                                                                                                                                                                                                                                                                                >  1:444646/1‑202 (MQ=255)
                                                                          aacaaATAATGAGGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGa                                                                                                                                                                                                     <  1:354520/275‑1 (MQ=255)
                                                                             aaaTAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGa                                                                                                                                                                                                                                                                            <  2:444645/202‑1 (MQ=255)
                                                                             aaaTAAGAATCACTGCCGACAACGTGCCTTTGCTAACCCCATCCATGCACGACTGCCAGATGCGTATTGACCGCCTTATCCCGCCTACGCATCGCGTCAAAATTTGTAGGTCTGTTAAGACGCGTTAGCTTCCCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAAc                                                                                                                                                                                                   <  2:204023/274‑1 (MQ=14)
                                                                                     gaggcggcgggggacggggaTTTGGTGTGCCCATCACATCGCGGCGGCCCGAGGCGGCGTGACCGCCGTGTGGGGCGTACGGATGGCGGGAGAATTGGGAGGGCTGATAAGACGCGTTGGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                                                           <  2:135266/255‑1 (MQ=11)
                                                                                     atgcCTGCCGAGAACGTGCATTTTGTATGCCCAACCCAGCACGACTGCCGGATGCGGCGTGAACGCCGTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                                                           <  1:115485/274‑1 (MQ=255)
                                                                                          tGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGAATACGGCATCTGCGAACGACTGCCGGAGGCGGCGTGGACGCCTTATCCGGCCt                                                                                                                                                                                     >  1:374258/1‑275 (MQ=255)
                                                                                                 aaCGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGACTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCGTTAGCGTAGCATCAGGAATCTGCGCACGACTGCCGGATGCGGCGTGAACGCGTTATCCGGCCTACggggt                                                                                                                                                                              >  1:527800/1‑273 (MQ=255)
                                                                                                     tggatgtggtgtGCCCATCCCAGCACGGCGGCCGGATGCGGAGTGAGCGCCTTATCCGGCCTACGGATGGCGGGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg                                                                                                                                                                           <  2:528819/263‑1 (MQ=255)
                                                                                                      gCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTCGCGTCGCATCAGGCAGCTTCGTCCGACGGCCGGACGCGGCGTTGACCACTTGTCCGGCCTACGGGTGgcg                                                                                                                                                                           >  2:126201/1‑273 (MQ=14)
                                                                                                            tGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCCCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGACCGACTGCCGGATGCGGCGTGAACCCCCTCTCCGGCCTACAGGTTGCGCGGGAAtt                                                                                                                                                                   >  2:293008/1‑275 (MQ=255)
                                                                                                                    cagcccAGCACGACGGCCGGATGCGGCGTGATCGCCGTATCCGGCCGACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGc                                                                                                                                                            <  1:271318/271‑1 (MQ=255)
                                                                                                                      tCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGAGGAGTGAACGCCTTATCAGGACTACGGATGGCGCGGGAATTGGTAGGCGTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGACGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAGTGTGTAGGCCTg                                                                                                                                                         >  1:69618/1‑275 (MQ=255)
                                                                                                                                 cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGAACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGTACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGCGTGGCGCGAGAACTTGTAGGCCTGATTAGACGCGt                                                                                                                                              >  1:454958/1‑275 (MQ=255)
                                                                                                                                      ggATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGTGAGAATTTGTGGGTCGGATAAGACGCGTTAGCGTGGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCg                                                                                                                                         <  2:32188/275‑1 (MQ=255)
                                                                                                                                       gagggggatggagcgcctgtgccGGCCTACTGAGGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGt                                                                                                                                        <  2:178539/254‑1 (MQ=14)
                                                                                                                                           cggggTGAACGCCTTATCCGGCCTACGGATGGCGGGAGAATTTTGAGGTCTGGTAGGACGCGTTAGGGTCGCATCAGGCACCTGCGCCCGACTGCCGGATGCGGCTTGAACGCCTTATCCGGCCTATGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCa                                                                                                                                    <  1:126202/275‑1 (MQ=11)
                                                                                                                                             gcgTGAACGCCTTTCGCGGCCTAGGCATGGCGTGGCAATTGTAGGGTCTGATAAGACGCGTTAGCGTCTCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCAt                                                                                                                                   <  2:69617/274‑1 (MQ=11)
                                                                                                                                              cgTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGCATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGAATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGGGTGGCGCGAGAGTTTGTAGGCCTGATAAGACGCGTAAGCGTCGCATcg                                                                                                                                 >  1:368094/1‑274 (MQ=255)
                                                                                                                                                                         ggCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTCTCCGGCCTACGGCTGGCGCGAGACGTTTTAGGCCTGATAAGACGCCGTAGCGTCGCATCTGGCATCTGCGCTCGACTGCCGGCTgcg                                                                                                      >  2:503621/1‑275 (MQ=14)
                                                                                                                                                                         ggCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGAGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAAGCCTGATAAGACGCGTTAGCGACGCATCAGGCATCTGCGCCCGACGGCCGGAcgcg                                                                                                      >  1:283765/1‑275 (MQ=255)
                                                                                                                                                                                                aTAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTTGGCACCACTGGCGGATTCGCAGTAAACGCCTTAGCCgccat                                                                                >  2:163342/1‑271 (MQ=14)
                                                                                                                                                                                                               gcggcggccggcagcgggggaCGACTGGCGGCTGCGGCGGTATCGCCTTATCCGGCCTACGGTGGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGGACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGa                                                                 <  2:368093/255‑1 (MQ=14)
                                                                                                                                                                                                                tCGCATCAGGCATGGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGCCCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGt                                                                                                                                                                               >  1:120075/1‑163 (MQ=11)
                                                                                                                                                                                                                 cGCATCAGGCATGGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGCCCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCgg                                                                                                           >  1:76564/1‑230 (MQ=11)
                                                                                                                                                                                                                    aTCAGGCATGGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGCCCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg                                                                                                                                                                           <  2:120074/163‑1 (MQ=11)
                                                                                                                                                                                                                     tCAGGCATGGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGCCCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATgc                                                                                                       <  2:76563/230‑1 (MQ=11)
                                                                                                                                                                                                                                       cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATCAGACGCGTTAGCGTCGCATCCGGCATCTGCGCACGACTGCCGGCTGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAAGTTGTAGGCCTggtcaggagcgt                                         >  1:307007/1‑264 (MQ=255)
                                                                                                                                                                                                                                          ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCGTAATAAGACGCGTTAGCGTAGCATCAGGCATCTCCGCACGACCGCCGGATTAGGCGGAAACGCCTCATTCGGCCTACTGACACCGGGGGAATTCATAGCACTGATAAGGCGCGGTa                                       >  2:474311/1‑274 (MQ=14)
                                                                                                                                                                                                                                               tGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTGAACCGGCCTACGGGTGGCGCGAGAATTTGTCGGCCTGATAAGACGCGTTAGCGTCACGTCAGGCATCTACGCACGACTGCCGGATGCGGCGTAAGCGCCTTATCCGGACTACGGATGGAGCGGGAACTTGTAGGCCTGATAAGACGCGTTCGCGCCg                                 >  1:525121/1‑275 (MQ=14)
                                                                                                                                                                                                                                                        aaccccgtgtgcggcgggcGGAGGGCGGGGGAATTTGGAGGCCGGAGAAGACGAGTTAGAGTCGCGTCAGGCATGTGCGCACGACTGCCGGATGCGGCGTGGACGCCTGATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGc                         <  2:499768/256‑1 (MQ=11)
                                                                                                                                                                                                                                                                ctCCGGCCTTCGGATGGCGCGGTAATTTGTAGGCCTTATAAGACGCTTTAGCTGCGCATCAGGCATCTGCGCACGACTGCCGGATGCGTCGTGAACGCCTTATCCTGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGcaca                <  1:461707/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                               tGGCGCGGGAATTTGTAGTCCTGATAAGACGCGTTAGCGGCGCATCAGGCATCCGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGc  <  1:325274/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                ggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTTGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGCCTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTCCGGATGGCGCGGGATCTTGTAGTCCTGATAAGACGCGTTAGCGCACCATCTGGCATCTGCAAACGACTGCTGGCTGc  >  1:509638/1‑274 (MQ=14)
                                                                                                                                                                                                                                                                                ggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGACTTATCCGGCCTCCGGTTGGCGCGTGCATTTGTAGGCCTGCTAAGACGCGTTAGCGTCGCATCAGGCAGCCGcaacaccccaccggatgc  >  2:262281/1‑258 (MQ=11)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                  
TTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGC  >  NC_000913/374799‑375342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: