Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	148.6 / NA	49	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/29);  total (20/29)

TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132652‑2132926                                                                                                                                        |                                                                                                                                             tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt                                                                                                                                       >  2:286367/1‑143 (MQ=255)      gagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCCGCGTCGCct                                                                                                                                  >  2:437723/1‑143 (MQ=255)            aaaCGGCTGATATTTTTTGCTGGCGCGGTCGATTGTGTCTTTGTGCTTGCGGTAAATGTGGAAGTACCCGGAGAATTTTTTCGTTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                            <  1:294428/143‑1 (MQ=255)            aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                            <  2:545330/143‑1 (MQ=255)            aaaCAGCTGATATTTCTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                            <  2:280392/143‑1 (MQ=255)               cAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                         <  2:491616/143‑1 (MQ=255)                     ttatTGTTTGCTGGCGCGTTCGAATTTGTCTTTGTGCCTGCGGTAAATGTGGAAGTACCCGGAGAATTTTTTCTGTGACGATGTTATCTGCATTTCACCGTGATTGATATCCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                                   <  1:452593/142‑1 (MQ=255)                      gattattTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg                                                                                                                  <  2:851008/142‑1 (MQ=255)                            ttccTGGCGCGGTCGGACTTGCCCTTGTGCGTGGGGTAAAAGTGGAAGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                            <  1:1127776/140‑1 (MQ=255)                               cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAACGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCTCCTCTCCTCTCCACGGTTCGCCGTa                                                                                                         >  2:529407/1‑143 (MQ=255)                                gggCGCGGTCGAATTGGTCTGTGTGCTTGCGGTAAAAGTGGGGGGGCCAGATTAATGGTGTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑c                                                                                                       <  2:873978/142‑1 (MQ=255)                                    gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTcca                                                                                                   <  2:851571/143‑1 (MQ=255)                                     cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTACAGTGACGAGGTCATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTccac                                                                                                  <  2:589137/143‑1 (MQ=255)                                       ggcGAATTGGTCCTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTccacca                                                                                                <  2:102152/141‑1 (MQ=255)                                              ttGTTTTCGTGCTTACTGTTTTAGTTGTAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACTGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCg                                                                                         <  1:66683/143‑1 (MQ=255)                                              ttGGCTCTGTGCGTGAGGTACAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCTCGGTTCGCCGTA‑CTCCACCACCATCCg                                                                                         <  1:990923/143‑1 (MQ=255)                                              ctGTCTTTGGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCg                                                                                         <  2:539402/142‑1 (MQ=255)                                                    ttGTGCTTGCGGCAAACGTGGCTGTTGCGTGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGTTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGaa                                                                                   <  1:571946/143‑1 (MQ=255)                                                          tggCGGTAACAGTGAATGGACCCGGCGTATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATGGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTc                                                                             <  1:412865/141‑1 (MQ=255)                                                               gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGCGATCTGCATTTCACCGTGATTGATAAGCAAGCTGTCCGACGCCTCTTCTCCTTTCCACGGTTCGCCGTG‑CTGCACCACCATACACAGGACGATGTCgcact                                                                        >  1:1047747/1‑139 (MQ=255)                                                                 aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTCACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATcc                                                                      <  2:1200132/143‑1 (MQ=255)                                                                    ggTGGAGGTACCCGGATAATTTTTTCGGTGACGAGGTGATCTGCATTTGTCCGTGATTGATATGCAGGCTCGGTGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                   <  1:198574/142‑1 (MQ=255)                                                                     gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTGGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTAGTAAGCCtgtg                                                                  >  2:437764/1‑143 (MQ=255)                                                                        gAAGTACCCGGAGAATTTTTTCGGAGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCCGCGTCGACTCTTCTCCTCTGCAAGGTTCGCCGTC‑CTCCAACACCATCCGCCGGAAGATGCCGCAAGCCTCTTCCg                                                               >  1:386573/1‑143 (MQ=255)                                                                          aGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCt                                                             <  2:88524/143‑1 (MQ=255)                                                                              cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                         >  2:404468/1‑143 (MQ=255)                                                                              cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCACGAAGATGTCGTAATCCGGTGCCGCTTCCa                                                         >  1:140868/1‑143 (MQ=255)                                                                               ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTGCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg                                                        >  2:608773/1‑143 (MQ=255)                                                                               ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTAACCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg                                                        >  2:754531/1‑143 (MQ=255)                                                                                 ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                      >  2:1201040/1‑143 (MQ=255)                                                                                  gtgaATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                     <  1:831200/141‑1 (MQ=255)                                                                                  gagaCTTTCTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTTCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                     <  1:151431/143‑1 (MQ=255)                                                                                    gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGG‑TCGATGAATAAACGCTTTCATCCGCAGGATGATGTCGTAATACTGTGCCGCTTTCAGTTCgg                                                   >  2:552355/1‑143 (MQ=255)                                                                                           ttCGGTGACGAGTGGATCTGCATTTCCCCGTGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                            <  2:431498/143‑1 (MQ=255)                                                                                               gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCGaacaaa                                        >  2:1009191/1‑143 (MQ=255)                                                                                                tgccgcggTGATCTGCATGCCACCGTGATTGATATGCAGGCTCGGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTA‑CTCCACCACCATGCGCGGGAAGATGTCTTCGTACTTTCCCGCTTTCAGTTCGGTATCGaacaaac                                       <  1:88790/137‑1 (MQ=255)                                                                                                tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                           >  2:180388/1‑123 (MQ=255)                                                                                                tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                           <  1:180388/123‑1 (MQ=255)                                                                                                  aCGGGGTGATCGGCATTTCACCGTGAGTGATATGCAGGCTCTGCGGCGCCGCGTCTACTTTCCACGGTGCGCCGTA‑TTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac                                     <  2:332877/143‑1 (MQ=255)                                                                                                    gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCACTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGCAAGATGTCGTAATCCTGTCCCGCCTTCAATCCGGTATCGAACAAacacac                                   >  1:341652/1‑141 (MQ=255)                                                                                                       gTGCTCTGCATCTCCCCTTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                                <  1:98205/143‑1 (MQ=255)                                                                                                       gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                                <  1:404468/143‑1 (MQ=255)                                                                                                       gTGAGCTGCATTTCACCGTGCTTGACATGCGGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                                <  2:217963/143‑1 (MQ=255)                                                                                                            cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACCAACACTCTTTGAAAc                           >  2:952090/1‑143 (MQ=255)                                                                                                                  ttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGCCGATGTCGTACTCCTGTGCCGCTTTCAGTTCGGTATCGAACCAACACTCTTTGACACGCCATg                     >  1:256069/1‑143 (MQ=255)                                                                                                                     aCCGTGCTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCCCCACCATCCGCAGCCAGATGTCGTACCCCTCTGCCGCTTTCCACTCGGTACCGCACAACCACTCTCTGACacgccacgcct                  >  1:217445/1‑142 (MQ=255)                                                                                                                             tgatATGCAGGCTCTGCGTCGCCTCTTATACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGACGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACACACACTCTTTGAAAGGCCATGCCCAGATAAAg          >  2:1154673/1‑143 (MQ=255)                                                                                                                                  tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg     <  1:754531/143‑1 (MQ=255)                                                                                                                                     aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACCCCATGCCCAGGTAAAGACCTGGtt  >  1:503031/1‑143 (MQ=255)                                                                                                                                        |                                                                                                                                             TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTA‑CTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132652‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑