Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA94.1% 197.5 / NA 70P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (40/24);  total (43/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA  >  NC_000913/4184400‑4184676
                                                                                                                                              |                                                                                                                                      
tatCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAccc                                                                                                                                        >  1:172572/1‑143 (MQ=255)
        ggTTAAAGCCCGTATCCAGCATGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGAGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAATGGTACGCCGGTAGACAAAGTACCGAACCAGCGGGGCg                                                                                                                                >  1:488340/1‑143 (MQ=255)
        ggTTAAACGCCGTATCCAGCCTAGTGACAAGATGGCAGGACGTCACGGTACCAAGGGCGTAATTTCTAAGAACCACCCGATAGAAGCTATGCCATACGATGAAAACGGTACGACGGTAGACATCGGACTGAAACAGCTGGCCg                                                                                                                                >  1:262791/1‑143 (MQ=255)
          ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                              >  2:718563/1‑143 (MQ=255)
          ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                              >  2:945819/1‑143 (MQ=255)
             aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCg                                                                                                                           >  2:598771/1‑143 (MQ=255)
              aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAACTGAGCGTACCga                                                                                                                          >  1:5636/1‑142 (MQ=255)
               cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGCTGAACACGGTACGCCGGTAGACATCGTACTCACCCAGCTGGGCCTAACGtc                                                                                                                         >  1:455154/1‑143 (MQ=255)
                 ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                       >  2:205986/1‑143 (MQ=255)
                  cGTATCCAGCCTGGTGACAAGAAGGTATGTCCGCACGGTACCAAGGGTTTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                      <  2:444627/143‑1 (MQ=255)
                    tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                    >  2:1037606/1‑143 (MQ=255)
                    gaTCCGGCCTGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                   <  1:1114565/142‑1 (MQ=255)
                     aTCCAGGCGGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATGTCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                   <  1:783368/143‑1 (MQ=255)
                      tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                  >  1:1069299/1‑143 (MQ=255)
                        cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                >  2:775995/1‑143 (MQ=255)
                         aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAc                                                                                                               >  2:262744/1‑143 (MQ=255)
                         aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTGCTAAGATCAACCCGGTCGAAGATATGCCATCCGATGAAAACGGTACGCCCGTAGACATCGTACTGAACCCGCCGGCCGTAcacgcacgtctgaac                                                                                                               >  1:359183/1‑129 (MQ=255)
                         aGCCTGGGGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAc                                                                                                               <  1:1126268/143‑1 (MQ=255)
                          gCCTGGTGCCAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACa                                                                                                              <  2:457693/143‑1 (MQ=255)
                            cTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATc                                                                                                            >  2:376889/1‑143 (MQ=255)
                             tGGTGACAAGATGGCAGGTCGTCACGGTAACAGGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACAAGCTGGGCGTACCGTCTCGTAAGAACATCg                                                                                                           >  1:628718/1‑143 (MQ=255)
                             tGGGGACAAGAGGGCAGGTCGTCACGGGAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                           <  2:207084/143‑1 (MQ=255)
                               gTGACAAGCGGGCCGGTCGGCTCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                         <  2:440742/143‑1 (MQ=255)
                                tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGGTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGAAGACATCGTACTGAACCAGCTGGAAGACCCTTCGCGTATGAACATGGGTc                                                                                                        >  1:895405/1‑143 (MQ=255)
                                tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                        <  2:506938/143‑1 (MQ=255)
                                         ggCAGGTCGGCACGGTAATAAGGGTGTAATTTCTAAGATCAGCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCg                                                                                               <  2:817826/143‑1 (MQ=255)
                                            aggtcctgaagtgaaCGAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATGTGCCTTACGATGAAAAGGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                            <  1:135405/130‑1 (MQ=255)
                                            aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGcaa                                                                                            >  1:403050/1‑143 (MQ=255)
                                              gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAcc                                                                                          <  2:361160/143‑1 (MQ=255)
                                                 gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                       >  2:987979/1‑143 (MQ=255)
                                                      ggTAACAAGGCTGTAATTTCTCATATCAACCCGATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGCACTGAACCAGCTGGGCGTACCGTCTCGTATGAACGTCGGTCAGATCCTCGAAACCCACCTggg                                                                                  <  1:984657/143‑1 (MQ=255)
                                                         aaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGACGGTAGACATCGTACTGAACCAGCTGGGAGTACCGTATCGTATGAACATCAGTCAGATCCTCGAAACCCACCTgggagt                                                                               >  1:476965/1‑140 (MQ=255)
                                                            aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGCACCAGCTGGGCGTACCGTCTCGTATGAACATGTGGCAGATCCTCGAAACCCCCCCGGGTATGGc                                                                            >  2:930505/1‑143 (MQ=255)
                                                            aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                            >  1:754246/1‑143 (MQ=255)
                                                            aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                            >  2:713295/1‑143 (MQ=255)
                                                                      tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACACCGGTAGACATCGTACTGAACCAGATGGGCGTACCCTATCGTATGTACTTCGGTCAGATCCTCGAAACCCACCCGGGTATGGCTGCGAAAGGt                                                                  >  1:1168904/1‑143 (MQ=255)
                                                                        tCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAt                                                                >  1:920056/1‑143 (MQ=255)
                                                                         cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc                                                               >  2:1038053/1‑143 (MQ=255)
                                                                         cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc                                                               >  2:1170561/1‑143 (MQ=255)
                                                                          tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                              >  2:1184147/1‑143 (MQ=255)
                                                                            aGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCGGGGTATGGCTGCGAAGGGTATCGGc                                                            >  2:169921/1‑143 (MQ=255)
                                                                              aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTGCCGTCTCGTATGAACATCGGTCAGATCGTCGAAACCCACCTGGGGATGGCCGCGCACGGTAgcggcgc                                                          >  1:1182747/1‑142 (MQ=255)
                                                                                 aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACAAGCTGGGCGTACCGTCTCGTATGAACAGCGGTCAGAGCCTCGAAGCCCGCCCGGGTCTGTCTGCGTAAGGTATCCGCGACaa                                                       >  2:239318/1‑143 (MQ=255)
                                                                                    ccGTTCAAAGATATGCCTTACGTTGGAACCGGTCCGCCGTTAGACATGGTACTGAACCAGCTGGGCGTACCGTCTCGTAGGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                    <  1:1113266/143‑1 (MQ=255)
                                                                                    ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                    <  2:371652/143‑1 (MQ=255)
                                                                                     cGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                   <  2:994023/143‑1 (MQ=255)
                                                                                         cGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGAACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGTATGGCGGCGAAAGGTATCGGCGACGAGCTCAACg                                               >  2:72823/1‑143 (MQ=255)
                                                                                             tatatGTCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAt                                           <  1:1027792/142‑1 (MQ=255)
                                                                                             gATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCGACAAGATCAACGCCAt                                           >  1:871061/1‑143 (MQ=255)
                                                                                                     ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                   >  2:76714/1‑143 (MQ=255)
                                                                                                        cGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                <  1:987979/143‑1 (MQ=255)
                                                                                                         gATGAAAACGGTACGCCGGTAGAAATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAACCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGACATGCTGAAAcagca                               >  1:374013/1‑143 (MQ=255)
                                                                                                          aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGCATCGGCGACAAGCTCAACGCCAGGCGGaagccgccg                              >  1:39421/1‑136 (MQ=255)
                                                                                                           tGAAAACGGTACGCCGGTAGACCTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGCGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                             <  2:627161/143‑1 (MQ=255)
                                                                                                           tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                             <  2:1104758/143‑1 (MQ=255)
                                                                                                              aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGAGCACAAGATCCACCCCATCCCGAAACCGCAGCgag                          >  1:510727/1‑143 (MQ=255)
                                                                                                                aCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGACCCTCGGTCAGCTCCTCGAAACCCACCAGGGTATGGCTGCGAAAGGAATCGGCGAAAAGATCAACCCCATGCTGAAATAGCAGCaagaa                        >  2:667681/1‑143 (MQ=255)
                                                                                                                aCGGTACGCCGGTAGACATCGTACGGAGCCGGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGTGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                        <  1:376889/143‑1 (MQ=255)
                                                                                                                 cGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                       <  2:172572/143‑1 (MQ=255)
                                                                                                                       gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGCATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa                 >  2:551195/1‑143 (MQ=255)
                                                                                                                        ccGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAGCGGTCAGATCCTCGAAACCCCCCCGGGTATGGCTGCGACAGGTCTCGGCGCCCAGACCAGCGCCAGGCCGAACCCgcagcaggcacgcgccga                >  1:890483/1‑131 (MQ=255)
                                                                                                                             aGACATCGTACTGAACCACCTGAGCGCACCTTCTCGTATAAACACCGGTCAGACCCTCGAACCCCACCTGGGTATGGCTGCGAAAGGTTTCGCCTACAAACTCAACGCCATGCTGAATCAGCAGCAAGAAGTCGCGAAACTgc           <  1:857266/143‑1 (MQ=255)
                                                                                                                             aGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAGCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc           <  2:198988/143‑1 (MQ=255)
                                                                                                                              gACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg          >  2:911882/1‑143 (MQ=255)
                                                                                                                                 aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCCGATCCTCGAAAACCACCTGCGTCAGGCTGCGAAAGGAATCGACGACAAGATCAGCCACCCGCGGAAACAGCAGCCAGAAGTCGCAAAACCGCACGa       >  1:926866/1‑143 (MQ=255)
                                                                                                                                 aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTGGCGAAACTGCGCGa       >  2:1043692/1‑143 (MQ=255)
                                                                                                                                  tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa      <  2:488340/143‑1 (MQ=255)
                                                                                                                                   cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt     >  2:206530/1‑143 (MQ=255)
                                                                                                                                    ggaCTGAACCCGCGGGGCGGACCGTCGCGTCTGAACATCGGTAAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAtt    <  1:812251/141‑1 (MQ=255)
                                                                                                                                     tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTc   <  2:1069299/143‑1 (MQ=255)
                                                                                                                                      gcTGAACCGGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCAGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa  <  1:205986/142‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                      
TATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA  >  NC_000913/4184400‑4184676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: