New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 232553 =59 (1.090)6 (0.120) 6/258 6.0 9.4% intergenic (+4/+44) yafE/mltD putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
?NC_000913 232584 = 62 (1.260)intergenic (+35/+13) yafE/mltD putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGCGTGAACATCAAAGATGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGACAAACCAGATAATAAAAAGGCACCGATTCCCCCGGTGCCTTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/232704‑232553
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggtgcctttttATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGC  >  NC_000913/232584‑232721
                                                                                                                                                                                                                                                                                                  
GGCGTGAACATCACAGATGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGACAAACCAGATAATAAAAAGGCACCGATTCCCCCGG                                                                                                                                                     <  1:852188/143‑1
 gcgtacACATCAAAGGTGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGACAAACCAGATAATAAAAAGGCACCGATTCCCCCGGT                                                                                                                                                    <  1:392760/137‑1
 GCGTGAACATCAAAGATGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGACAAACCAGATAATAAAAAGGCACCGATTCCCCCGGT                                                                                                                                                    <  2:687109/143‑1
  CGTGAACATCAAAGATGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGACAAACCAGATAATAAAAAGGCACCGATTCCCCCGGTG                                                                                                                                                   <  2:412332/143‑1
  CGTGAACATCAAAGATGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGACAAACCAGATAATAAAAAGGCACCGATTCCCCCGGTG                                                                                                                                                   >  2:381251/1‑143
     GAACATCAAAGATGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGACACACCAGATAATAAAAACGCACCGATTCCCCCGGTGCCT                                                                                                                                                >  1:839220/1‑143
         ATCAAAGATGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGTCTAAAAGCTGACGTTGTTTGTAACAAACAACAACATGCCAGCTTCCTGACAAACCAGATCATAAAAATGCACCGACTCCCCCcgccactctct                                                                                                                                            >  2:909933/1‑132
         ATCAAAGATGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGACAAACCAGATAATAAAAAGGCACCGATTCCCCCGGTGCCTTTTT                                                                                                                                            <  2:746883/143‑1
         ATCAAAGATGTGATGCGCTGGAACAGAGACACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGACAAACCAGATAATAAAAAGGCACCGATACCCCCGGTGCCTTTTT                                                                                                                                            >  1:281156/1‑143
                                                                                                                CCAGATAATAAAAAGGCACCGATTCCCCCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCAC                                     >  2:881719/1‑143
                                                                                                                   GATAATAAAAAGGCACCGATTCCCCCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTG                                                         >  1:92035/1‑120
                                                                                                                   GATAATAAAAAGGCACCGATTCCCCCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTG                                                         <  2:92035/120‑1
                                                                                                                        TAAAAAGGCACCGATTCCCCCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGA                             >  1:94645/1‑143
                                                                                                                        TAAAAAGGCACCGATTCCCCCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGA                             <  1:881719/143‑1
                                                                                                                           AAAGGCACCGATTCCCCCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTAGCAGTGTCGCTGCTCCAGCGCATCACATCTTTGATGT                          >  1:513616/1‑143
                                                                                                                                            ctgtcCCTTTTTTTTAGCGGGTGCGTCAGTGATCTGGCATGGTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACAGCTTTGATGTTCACGCCGTGGCGTTTA         <  1:216780/138‑1
                                                                                                                                            CGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCGTATCGCCTGGTGGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTA         <  2:634617/143‑1
                                                                                                                                              GTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTGGCTGGTCCAGCGCATCAGATCTTTGCTGTGCACGCCGAGGAGTGTAGC       >  1:853515/1‑143
                                                                                                                                              GTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGCCGTTTAGC       >  2:463766/1‑143
                                                                                                                                                 CCTTTTTATTATCTCGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCTGATTCGCCCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAAT    <  1:423516/143‑1
                                                                                                                                                   TTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCCGCGCATCCCATCTTTGATGTTCCCGCCGTGGCGTctcccactgc  >  2:476562/1‑133
                                                                                                                                                   TTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGC  <  2:85758/143‑1
                                                                                                                                                                                                                                                                                                  
GGCGTGAACATCAAAGATGTGATGCGCTGGAACAGCGACACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGACAAACCAGATAATAAAAAGGCACCGATTCCCCCGGTGCCTTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/232704‑232553
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggtgcctttttATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGC  >  NC_000913/232584‑232721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.