| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 2024155 = | 3 (0.060) | 60 (1.110) | 49/282 | 0.1 | 95.2% | coding (188/624 nt) | rcsA | transcriptional regulator of colanic acid capsular biosynthesis |
| ? | NC_000913 | 2101749 = | NA (NA) | intergenic (‑5/+146) | wbbL/insH1 | pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator | |||||
GTTTAATCGATTTAGAACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913/2024295‑2024155‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT > NC_000913/2101749‑2101885 GTTTAATTGATTTAGAACTGATCAATAAATGTTTGCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGG < 1:185432/143‑1 TTTAATCGATTTAGAACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGA > 2:96414/1‑143 TAATCGATTTAGAACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAG < 2:475648/143‑1 ATCGATTTAGAACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACGGTTAGGGAAGGT > 1:44568/1‑143 ATCGATTTAGAACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGAATTGGGATGTTGATTAATGATGAGCTTGATACGCTGAATGTTAGGGAAGGT > 1:812742/1‑143 TCGAGTTAGAACGGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTG > 2:587104/1‑143 TTTAGAACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAACACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAA > 1:650350/1‑143 TAGAACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGGCGTTTAGGGCAGGTGCGAATA > 1:821050/1‑143 AGAACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAA < 2:45657/143‑1 ACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCG < 2:806106/143‑1 ATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGA > 1:245276/1‑143 ATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGAGTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGGGGGAGTAAGCGAGGg > 2:314056/1‑142 ATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACAATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGA > 2:663014/1‑143 TTCTGACCAATTGATACTCATCAAAATGAACATTGGCAGTTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGC < 1:811140/143‑1 GACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAC > 1:298701/1‑143 CCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTC > 2:580143/1‑143 CAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGTTGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCA > 2:746424/1‑143 CAATAGATATTCATCAAAATGAACAGTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCA < 1:839731/143‑1 CAAGAGAGATTGAGCAAAATGAACATTGGCAATGGCCATAAAAACGATAAATGACGGATTGGGATGGTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCA < 2:247015/143‑1 ATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGT > 2:284337/1‑143 ATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTC > 1:448775/1‑143 CATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATT > 2:33960/1‑143 TCAAAATGAACATTGGCAGTTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGCCATTTCATTTC > 1:207413/1‑143 TCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAGCAGTCCACGGTTGACTCAGTCATTTCATTTC > 2:851274/1‑143 TCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTC > 2:364239/1‑143 CAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGACGCTGATTAATGAAGAGCTTGATACGCTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAACTTCCTCTCGGCTGACTCCGCCACTTCActccc > 1:487183/1‑138 ACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTT < 2:105912/143‑1 CATTGGCAATTGCCATAAAAACGATAAATACCGTATTTGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTG < 1:880417/143‑1 CATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGCAATTGGCCCCACCTGACTGAGTCATTTCCTTTGTTCATGCTTG > 1:420209/1‑143 TTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAG > 2:935071/1‑143 TGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCGTTTCTTCATGTTTGAGC > 2:893549/1‑143 TTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAACTTCTTCGCAGCTGACTCAGGCATTTAATTTCGTCATGTTTGAGCCGATTT > 1:523025/1‑143 TGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTT < 1:587104/143‑1 aCATAACAACGGTAAATAGCGTATTGGTATGTTTATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATGAGCGGGGAAATTCTTCCCGTCTGACTCATTCATTTCATTTCTTCATGTTTGAGCCGATTTTTT < 1:868446/142‑1 CCATAAAACCGCCAAATAACGTATGGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATGTCATTTCTTCATGTTTGAGCCGATTTTTT < 2:283358/143‑1 CCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTT < 1:96414/143‑1 CCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTT > 1:91428/1‑143 CATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGCGCTTGATACGCTGACTGTTAGGGTAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCCGTCATTTCATTTCTTCTTGTTTGCGCCGATTTTTTg > 2:159577/1‑142 ATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCT < 1:580143/143‑1 TAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCCTGTTTGAGCCGATTTTTTCTC > 1:335337/1‑143 acgACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCC < 2:76957/140‑1 AAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCG > 2:888472/1‑143 tacCGTATTGGTATGTTGATTAATGATGAGCTTGATCCGCTGACTGTTATGGAAGGTGCGAATACGCGGGTAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTT < 1:771667/140‑1 TAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATATGCTGGGAATTTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTT < 1:631704/143‑1 TAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGACTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAACTGCCTT > 1:471143/1‑143 ACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATCTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGA > 2:489219/1‑143 TGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGTGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGC < 2:451822/143‑1 GGATGTTGATTCATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGATTGAGCCGATCTTTTCTCCCGTAAATGCCTTGAATCCGCtt > 1:50513/1‑141 GGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGAGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCT > 1:577497/1‑143 ATTAATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAC < 2:91428/143‑1 ATGATGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTT > 2:609247/1‑143 TGAGCTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTT < 2:298701/143‑1 tAGCTTGTTACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTC < 1:655686/142‑1 CTTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCC < 2:382434/143‑1 TTGATACGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA > 2:667116/1‑143 TTGAGACGCTGACTGTTAGGGAAGGTTGGAATAAGCGCGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA < 1:893549/143‑1 CGCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAG < 1:284337/143‑1 GCTGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTCAAGG > 1:349862/1‑143 TGACTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTCCGCCATTTAAGGCG > 2:707287/1‑143 TGTTAGGTAAGGTGCGAAGAAGCGGGGAAATTCTTCTCGGCTGCCTCAGTCACTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT < 2:917984/143‑1 GTTTAATCGATTTAGAACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913/2024295‑2024155‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT > NC_000913/2101749‑2101885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |