| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,509,495 | C→G | intergenic (‑71/‑133) | glk ← / → yfeO | glucokinase/putative ion channel protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,509,495 | 0 | C | G | 98.0% | 145.1 / NA | 49 | intergenic (‑71/‑133) | glk/yfeO | glucokinase/putative ion channel protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (20/28); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACAT > NC_000913/2509356‑2509629 | ggCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGcc < 1:125230/143‑1 (MQ=255) aaTATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGa > 2:875299/1‑143 (MQ=255) cacacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCCCCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAAGAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAaggc < 1:337070/143‑1 (MQ=255) acacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAaggca > 1:159801/1‑143 (MQ=255) acaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAaggcagg > 2:849669/1‑143 (MQ=255) agCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATCACACCAAGGCCGGAACGCCGGGCTcc > 2:48617/1‑143 (MQ=255) aGACGTGCGTTGGTGCCGCCCACATTACCGACTAATGCATACTCTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAACTACCTGGGGACATAACAACCAGGCCGGACAGGCAGGCTCCCTGt > 1:687132/1‑143 (MQ=255) gTGCGTTGGTGCCGCCCCCACCACCGACTAATGCACACTCTGTCATTCCTCAACTGCTCCGCCAAAG‑CCAAAATAACGCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAat < 2:307154/143‑1 (MQ=255) tGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAata < 2:18562/143‑1 (MQ=255) gCGTTGGTGCCGCCCACACCACCGACTAATGCATACTTTCTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAatat < 2:650224/143‑1 (MQ=255) gTTGGTGCCTCCCACAGCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAAGTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCg < 1:697401/143‑1 (MQ=255) tGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGAt < 2:808883/143‑1 (MQ=255) gcggccCAGATCACCGACTGAGGCATACGTGGTCAGTGTTCAACGGCTCCGCTAAAG‑TCAAGATAATTCTGGCTCAGACTGTATATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTgg < 2:684017/140‑1 (MQ=255) cccACATCACCTACTAATGCATACTTTGTCATTCTTCAACTGCTCTG‑TATTGATCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTca < 2:216862/143‑1 (MQ=255) tccccGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAAt < 2:805550/140‑1 (MQ=255) tacccGACTAGTGCATACTTTGTCATTCTTCAACTGCGCCGCTAAAG‑TCAAAATAATTCTTTCTCACACGGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAAt < 1:436224/140‑1 (MQ=255) cGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACt < 2:467377/143‑1 (MQ=255) aCTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACttt > 2:116473/1‑143 (MQ=255) cTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAGCAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTa > 2:442111/1‑143 (MQ=255) gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGtt > 2:474246/1‑143 (MQ=255) aCTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAg < 2:765493/143‑1 (MQ=255) tCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCaa > 1:297685/1‑143 (MQ=255) tCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCaa > 2:164331/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAtt > 2:103068/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGACACACGATTACTTTATGGTCTCCGCagctcta > 2:943206/1‑137 (MQ=255) aTTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATAACGATCTGGGTCACACAATTACTTCATCgagacagaccccatt > 1:567620/1‑129 (MQ=255) cttcAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCa > 1:373523/1‑143 (MQ=255) tcAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGTCCGGAAAGGCAGGCTCCCTGTAAATATAGATCTGGGTCACACAATTACCTTATCGTTTCAGCACCAATTGCAGc > 1:352161/1‑143 (MQ=255) caCTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGa < 2:477334/142‑1 (MQ=255) tGCCCCGCTAAAG‑CCACAACAAGTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGTCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc < 2:15385/143‑1 (MQ=255) tCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCtt > 2:840889/1‑143 (MQ=255) gCTAATG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCttttt < 1:253964/143‑1 (MQ=255) cTAAAG‑TCAAAATAATTCTTTCTCACACAGCCCGGAGCTGCGCGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTg < 1:925091/143‑1 (MQ=255) tCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCt < 2:159801/143‑1 (MQ=255) tCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGACGCCTCTTCGCAAGCt > 1:7837/1‑143 (MQ=255) acataatTGTGTCTCACCCGTTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTggg < 1:813257/141‑1 (MQ=255) aaaTAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGGTggg > 2:736974/1‑143 (MQ=255) tattctCACATGGTAAATTCCTGGGGGCATAACGACACGGCCGGAATGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa < 1:624132/141‑1 (MQ=255) tttctCACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAg < 1:474246/143‑1 (MQ=255) ctcACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTAt < 2:373523/143‑1 (MQ=255) tcACACTGTAAATACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCCCCCTGTAAATATCGATCTGGGTCACACAATTACTTTACCGTTTCAGCACAAATCGCAGCGATGCCTCTTTGCAAGCTGGGCACACTAAGTagc > 1:248804/1‑141 (MQ=255) cattGTAAATTCCTTGGGGCATAACAACAAGTCTGTAAAGGTAGGCTCCCTGTAAATATCGATCTGGGTCACATAATTACTTTATCTTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGa < 1:663947/140‑1 (MQ=255) cTGGAAAGACCCGGGTGCATAACCACTGGGCCGGAAATGTGGTCGTCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAcc < 1:925701/143‑1 (MQ=255) tACCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATa < 1:116473/143‑1 (MQ=255) aCCTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATAGATATGGGGCACAAACTTACTTTACTGATTCAGCACCAATTGCAGCGATGCCATTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATac > 1:833012/1‑142 (MQ=255) tcTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAg < 1:103068/142‑1 (MQ=255) cTGGGGGCATAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATaatg > 1:760521/1‑141 (MQ=255) ggCATAACACCAGGGCCGGAAAGGAAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAg < 2:157955/143‑1 (MQ=255) tAACAACAAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACa < 2:7837/143‑1 (MQ=255) aacaacaagGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAAAAt > 2:5866/1‑143 (MQ=255) | GGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAG‑TCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACAT > NC_000913/2509356‑2509629 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |