Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	2,023,805	(CTTTA)2→1	67.9%	intergenic (+127/‑159)	fliR → / → rcsA	flagellar export pore protein/transcriptional regulator of colanic acid capsular biosynthesis

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2023801 =	14 (0.230)	28 (0.470)	24/474	NT	67.9%	intergenic (+123/‑167)	fliR/rcsA	flagellar export pore protein/transcriptional regulator of colanic acid capsular biosynthesis
	?	NC_000913	= 2023795	13 (0.220)					intergenic (+117/‑173)	fliR/rcsA	flagellar export pore protein/transcriptional regulator of colanic acid capsular biosynthesis

TTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTT‑AAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCT  >  NC_000913/2023536‑2024083                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                             ttttgttattggatttccattaactctgactgtcggcatctctttaatggcggcattaatgccgttaattgcacctttttgcgaacatttattcagtgaaatttttaatttgctggctgatattattagtgaattgcaattaatataattccgtaacgtttatcatgttatcctaaggattatccgacaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagatcactcacctatcgcaacaTTTACTTTAAGAcaattcccggac                                                                                                                                                                                                                                                                       <  1:367975‑M1/24‑6 (MQ=255)           ggatttccattaactctgactgtcggcatctctttaatggcggcattaatgccgttaattgcacctttttgcgaacatttattcagtgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATacaa                                                                                                                                                                                                                                                             >  1:29502‑M1/256‑289 (MQ=255)                     taactctgactgtcggcatctctttaatggcggcattaatgccgttaattgcacctttttgcgaacatttattcagtgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACgg                                                                                                                                                                                                                                                   >  2:92354‑M1/246‑289 (MQ=255)                            tCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTa                                                                                                                                                                                                                                            >  2:272044/1‑289 (MQ=255)                            gactgtcggcatctctttaatggcggcattaatgccgttaattgcacctttttgcgaacatttattcagtgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTa                                                                                                                                                                                                                                            <  2:277936‑M1/51‑1 (MQ=255)                                 tcggcatctctttaatggcggcattaatgccgttaattgcacctttttgcgaacatttattcagtgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACgg                                                                                                                                                                                                                                                   <  2:272116‑M1/44‑1 (MQ=255)                                 tcggcatctctttaatggcggcattaatgccgttaattgcacctttttgcgaacatttattcagtgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACgg                                                                                                                                                                                                                                                   >  1:272116‑M1/234‑277 (MQ=255)                                                                gttaattgcacctttttgcgaacatttattcagtgaaatttttaatttgctggctgatattattagtgaatttccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCaaa                                                                                                                                                                                                                                                                     <  2:384060‑M1/26‑1 (MQ=255)                                                                gttaattgcacctttttgcgaacatttattcagtgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCaaa                                                                                                                                                                                                                                                                     >  1:384060‑M1/203‑228 (MQ=255)                                                                 tGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGt                                                                                                                                                                                                       <  1:272044/289‑1 (MQ=255)                                                                    attgcacctttttgcgaacatttattcagtgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGAc                                                                                                                                                                                                    >  1:277936‑M1/199‑289 (MQ=255)                                                                             ttttgcgaacatttattcagtgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCAtgttgt                                                                                                                                                                                           <  1:93959‑M1/100‑1 (MQ=255)                                                                                  aTTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCAAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCATTGTTGTTCCGt                                                                                                                                                                                      >  1:151985/1‑289 (MQ=255)                                                                                        tttattcagtgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTc                                                                                                                                                                                >  2:387194‑M1/179‑289 (MQ=255)                                                                                                 tgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATta                                                                                                                                                                                                                                                                  >  1:304496‑M1/170‑198 (MQ=255)                                                                                                 tgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATta                                                                                                                                                                                                                                                                  <  2:304496‑M1/29‑1 (MQ=255)                                                                                                 tgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCa                                                                                                                                                                                                             <  1:257551‑M1/82‑1 (MQ=255)                                                                                                 tgaaatttttaatttgctggctgatattattagtgaattgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCa                                                                                                                                                                                                             >  2:257551‑M1/170‑251 (MQ=255)                                                                                                                                      ttgccattaatataattccgtaacgtttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATtgagtg                                                                                                                                 >  2:93959‑M1/133‑289 (MQ=255)                                                                                                                                                     tAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAg                                                                                                                                                                                                                                                                          >  1:534461/1‑138 (MQ=255)                                                                                                                                                     tAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAg                                                                                                                                                                                                                                                                          <  2:534461/138‑1 (MQ=255)                                                                                                                                                     tAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTc                                                                                                                  >  2:105088/1‑289 (MQ=255)                                                                                                                                                     tAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTc                                                                                                                  >  2:253510/1‑289 (MQ=255)                                                                                                                                                        cGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAAc                                                                                                               <  1:253510/289‑1 (MQ=255)                                                                                                                                                          taacgtttatcatgttatcctaaggattatcagaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGa                                                                                                             <  1:77590‑M1/177‑1 (MQ=255)                                                                                                                                                                ttatcatgttatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAAGTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGAttatta                                                                                                       >  2:489910‑M1/107‑289 (MQ=255)                                                                                                                                                                         tatcctaaggattatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTAt                                                                                              >  2:367975‑M1/98‑289 (MQ=255)                                                                                                                                                                                     tatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGAttatta                                                                                                       <  1:65827‑M1/183‑1 (MQ=255)                                                                                                                                                                                     tatccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGAttatta                                                                                                       >  2:65827‑M1/86‑268 (MQ=255)                                                                                                                                                                                       tccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCg                                                                                 <  1:356531‑M1/205‑1 (MQ=255)                                                                                                                                                                                       tccgaaaaataatacctacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCg                                                                                 <  2:358323‑M1/205‑1 (MQ=255)                                                                                                                                                                                                       tacgaacatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGt                                                                >  1:358323‑M1/68‑289 (MQ=255)                                                                                                                                                                                                            acatcttccaggatactcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTg                                                           >  2:356531‑M1/63‑289 (MQ=255)                                                                                                                                                                                                                     aCTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTaga                                                  <  1:105088/289‑1 (MQ=255)                                                                                                                                                                                                                       tCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATgttgtt                                                                                                                                                                                          >  2:68956/1‑152 (MQ=255)                                                                                                                                                                                                                       tCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATgttgtt                                                                                                                                                                                          <  1:68956/152‑1 (MQ=255)                                                                                                                                                                                                                       tCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAgg                                                >  1:150167/1‑289 (MQ=255)                                                                                                                                                                                                                         cTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAgggg                                              >  1:528521/1‑289 (MQ=255)                                                                                                                                                                                                                          actcctgcagcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGt                                             <  2:336872‑M1/241‑1 (MQ=255)                                                                                                                                                                                                                                   gcgaaatatttgttttaagctcactcacatatcgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTT‑AAAAAAag                                   <  2:163330‑M1/250‑1 (MQ=255)                                                                                                                                                                                                                                                                   cgcaacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACACGACTAGGTTTAACCGGGTATCTGTTGAGTAGCGGGGTT‑AAAAAAAGAGAATTCAACGAAATTGAAACCGTtgatga     <  1:368386‑M1/280‑1 (MQ=255)                                                                                                                                                                                                                                                                    aTTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAAGAGAAATCAACGACATTGAAACCGTtgatga     >  2:26289/1‑287 (MQ=255)                                                                                                                                                                                                                                                                     caacaTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTT‑AAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCt  <  2:113810‑M1/284‑1 (MQ=255)                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                             TTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCACATATCGCAACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATG‑AAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTT‑AAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCT  >  NC_000913/2023536‑2024083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.