Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,573,988 G→A 6.1% pseudogene (69/2937 nt) yjiV → pseudogene, conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,573,9880GA 6.1% 222.4 / 3.7 66pseudogene (69/2937 nt)yjiVpseudogene, conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/33);  new base A (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

TATTAGGACTAAAAGCACTACCTGTGAAGGGATGTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGAAAATAAAG  >  NC_000913/4573700‑4574268
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                        
tATTAGGACTAAAAGCACTACCTGTGAAGGGATGTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAggg                                                                                                                                                                                                                                                                                          >  1:364143/1‑289 (MQ=255)
   tAGGACTAAAAGCACTACCTGTGAAGGGATGTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACt                                                                                                                                                                                                                                                                                       <  1:395412/289‑1 (MQ=255)
        cTAAAAGCACTACCTGTGAAGGGATGTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAAcc                                                                                                                                                                                                                                                                                  <  1:246000/289‑1 (MQ=255)
        cTAAAAGCACTACCTGTGAAGGGATGTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCAGTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAAcc                                                                                                                                                                                                                                                                                  <  1:505814/289‑1 (MQ=255)
                              gATGTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGTGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCAttt                                                                                                                                                                                                                                                            >  2:546390/1‑289 (MQ=255)
                              gATGTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCAt                                                                                                                                                                                                                                                              <  2:218842/287‑1 (MQ=255)
                               aTGTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCAtt                                                                                                                                                                                                                                                             >  2:258997/1‑287 (MQ=255)
                                        cGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCa                                                                                                                                                                                                                                                  <  1:145114/289‑1 (MQ=255)
                                            acagttacagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGgc                                                                                                                                                                                                                                              <  2:364143/289‑1 (MQ=255)
                                            acagttacagCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGgc                                                                                                                                                                                                                                              <  1:343199/289‑1 (MQ=255)
                                                        aaTGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTc                                                                                                                                                                                                                                                   <  1:436557/272‑1 (MQ=255)
                                                        aaTGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTc                                                                                                                                                                                                                                                   >  2:436557/1‑272 (MQ=255)
                                                                    cGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTa                                                                                                                                                                                                                      <  1:258997/289‑1 (MQ=255)
                                                                          cGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCGGGGCGCATATCTTCCAGCGTAATACCTAGGGAat                                                                                                                                                                                                                >  2:226960/1‑289 (MQ=255)
                                                                                            aTCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATaa                                                                                                                                                                                              >  1:132252/1‑289 (MQ=255)
                                                                                            aTCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATaa                                                                                                                                                                                              >  2:359347/1‑289 (MQ=255)
                                                                                                      gAAGGTTGGGTCTAGATGGAAAGCGTGCAAGTTTGGCTTCTCATCCAGATGAAACATTTATTTGTGTACCAAATGTCATTTCTACACTACCGGATATTCAAACCATTTATGTAACGCGTTAGACGTGCTGAAAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATGGTTGTCTGTTCAGGGAATGAACTTAATGCAATGGATCCCGCTTTTCTGAAAGTAAGGGCGCATATCTTCCAGCGTGATACTTAGGGAATATGGGCTTGTGTTGATAAAGAtttccat                                                                                                                                                                                    >  1:452107/1‑284 (MQ=255)
                                                                                                            ttGCTTTAAATGGAAAGGTGTCAAGCTGGGTTTGTCAACCAGATGAAACATTTATTGGTGTACCAAAAGGCATTTCTACACTACCGGATATTCAAAACATTGGTGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTaaa                                                                                                                                                                               <  1:359347/288‑1 (MQ=255)
                                                                                                             tGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACt                                                                                                                                                                                                                                                                                       >  2:53145/1‑183 (MQ=255)
                                                                                                             tGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACt                                                                                                                                                                                                                                                                                       <  1:53145/183‑1 (MQ=255)
                                                                                                                         aaaGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAGGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACATCCAGCATGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCTCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGc                                                                                                                                                                 <  1:347294/289‑1 (MQ=255)
                                                                                                                          aaGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCGGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGc                                                                                                                                                                 <  1:226960/288‑1 (MQ=255)
                                                                                                                              gTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGAc                                                                                                                                                            >  1:187380/1‑289 (MQ=255)
                                                                                                                                gCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACa                                                                                                                                                           <  1:41927/288‑1 (MQ=255)
                                                                                                                                       tGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCt                                                                                                                                                     <  2:187380/288‑1 (MQ=255)
                                                                                                                                        gggTTTGTCAACCAGATGAAACATTTAGTGGTGTACCAAAAGGCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTgg                                                                                                                                                   <  1:541497/289‑1 (MQ=255)
                                                                                                                                          gTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCAt                                                                                                                                                                                                                                                              <  2:65185/180‑1 (MQ=255)
                                                                                                                                          gTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCAt                                                                                                                                                                                                                                                              >  1:65185/1‑180 (MQ=255)
                                                                                                                                            ttGTCATCCAGATGAAACCTTTATTTGTGTACAAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACTTGTAGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCt                                                                                                                                               <  1:546390/289‑1 (MQ=255)
                                                                                                                                                cATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTgg                                                                                                                                           <  1:569582/289‑1 (MQ=255)
                                                                                                                                                                 tATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCa                                                                                                                                                                            <  1:306069/239‑1 (MQ=255)
                                                                                                                                                                 tATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCa                                                                                                                                                                            >  2:306069/1‑239 (MQ=255)
                                                                                                                                                                     ggTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGgc                                                                                                                                                                                                                                              >  1:224026/1‑169 (MQ=255)
                                                                                                                                                                     ggTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGgc                                                                                                                                                                                                                                              <  2:224026/169‑1 (MQ=255)
                                                                                                                                                                        gtACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACa                                                                                                                   >  2:224061/1‑289 (MQ=255)
                                                                                                                                                                         tACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGc                                                                                                                                                                                                          >  2:558671/1‑201 (MQ=255)
                                                                                                                                                                         tACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACaa                                                                                                                  >  1:226115/1‑289 (MQ=255)
                                                                                                                                                                         tACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACaa                                                                                                                  >  2:274927/1‑289 (MQ=255)
                                                                                                                                                                         tACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGGGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGc                                                                                                                                                                                                          <  1:558671/201‑1 (MQ=255)
                                                                                                                                                                                 gTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGAAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATtgtg                                                                                                           >  2:471000/1‑288 (MQ=255)
                                                                                                                                                                                            acTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGcc                                                                                               <  2:226115/289‑1 (MQ=255)
                                                                                                                                                                                                   gATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGaa                                                                                                  <  1:180953/279‑1 (MQ=255)
                                                                                                                                                                                                   gATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGaa                                                                                                  >  2:180953/1‑279 (MQ=255)
                                                                                                                                                                                                   gATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTAAACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGtata                                                                                        >  1:502814/1‑289 (MQ=255)
                                                                                                                                                                                                        tCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGcc                                                                                                                                                 <  2:536024/227‑1 (MQ=255)
                                                                                                                                                                                                        tCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGcc                                                                                                                                                 >  1:536024/1‑227 (MQ=255)
                                                                                                                                                                                                        tCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAg                                                                                   >  2:27003/1‑289 (MQ=255)
                                                                                                                                                                                                               aTTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCa                                                                              <  1:27003/287‑1 (MQ=255)
                                                                                                                                                                                                               aTTGAGGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCAtt                                                                            <  2:452107/289‑1 (MQ=255)
                                                                                                                                                                                                                                     gCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTTGAAGCCCTGTATACGAAGTTGCATTCTTTAATGATTGTAATGAGcc                                                       >  1:46852/1‑288 (MQ=255)
                                                                                                                                                                                                                                     gCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGAACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGcc                                                       >  1:111484/1‑288 (MQ=255)
                                                                                                                                                                                                                                       tGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCa                                                    <  2:132252/289‑1 (MQ=255)
                                                                                                                                                                                                                                             aaCAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACg                                                                                      <  1:346217/249‑1 (MQ=255)
                                                                                                                                                                                                                                             aaCAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACg                                                                                      >  2:346217/1‑249 (MQ=255)
                                                                                                                                                                                                                                                   aTTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCtatg                                                                                                                                      >  2:422881/1‑195 (MQ=255)
                                                                                                                                                                                                                                                   aTTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCtatg                                                                                                                                      <  1:422881/195‑1 (MQ=255)
                                                                                                                                                                                                                                                          ccagcAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACaaa                                 >  2:168699/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           cagcagGAAGTACTGCGTTGGATCGATGTCTGTTCAGGAACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACaaa                                 <  2:111484/288‑1 (MQ=255)
                                                                                                                                                                                                                                                            agcagGAAGTACTGCGTTGGATCGATGTCTGTTCAGGAACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGAc                                                                                                         <  1:33295/215‑1 (MQ=255)
                                                                                                                                                                                                                                                            agcagGAAGTACTGCGTTGGATCGATGTCTGTTCAGGAACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGAc                                                                                                         >  2:33295/1‑215 (MQ=255)
                                                                                                                                                                                                                                                              cagGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCa                                                                              >  1:33482/1‑240 (MQ=255)
                                                                                                                                                                                                                                                              cagGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCa                                                                              <  2:33482/240‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCt                                                                                                                                         <  2:369232/170‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCt                                                                                                                                         >  1:369232/1‑170 (MQ=255)
                                                                                                                                                                                                                                                                                    aTGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTtgtg                                                                                                                                                                                                     >  1:95888/1‑99 (MQ=255)
                                                                                                                                                                                                                                                                                    aTGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTtgtg                                                                                                                                                                                                     <  2:95888/99‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         tGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGAAAATAAAg  >  2:167335/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                        
TATTAGGACTAAAAGCACTACCTGTGAAGGGATGTCAGGACGTTACAGTTACAGCAAATGAAACTTATCGGATTCGCACCGGAAGAGAACAAATCAGCATCGGAAGGTTTGCTCTAAATGGAAAGCGTGCAAGCTGGGTTTGTCATCCAGATGAAACATTTATTGGTGTACCAAAAGTCATTTCTACACTACCGGATATTCAAAGCATTGATGTAACGCGTTACACGTGCTGACAAAACAGCATTACAGCCAGCAGGAAGTACTGCGTTGGATCGATGTCTGTTCAGGGACTCAACCTAATGCAAAGGATCCCGCATTTCTTAAAGTCAGGGCGCATATCTTCCAGCGTAATACCTAGGGAATATGGGCTTGTGTTGATAAAGATTGCAGATTAAAGCACGGTACACCGCTCGACAAAGGCTGGCCCTTTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGAAAATAAAG  >  NC_000913/4573700‑4574268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: