Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 83.7 / NA 29I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/15);  total (14/15)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132650‑2132925
                                                                                                                                         |                                                                                                                                          
cccggTAGAGGGCACACAGCTGGTATTTTGTGTTTTCGCGGCCGGTTTTTTCGTTGTTCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGATGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                       <  1:282798/140‑1 (MQ=255)
   tgTAGAGGTTAAACAGCTGATATTTTTTGCTGGTGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                    <  1:307222/142‑1 (MQ=255)
   ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATCTTTTCGGTGACGAGGTGATGTGCATTTCACCGTGATTGAGATGCAGGCTCTGCGTc                                                                                                                                    >  1:423496/1‑143 (MQ=255)
    gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                   >  1:221053/1‑143 (MQ=255)
     tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  <  2:614923/143‑1 (MQ=255)
     tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGAAACGAGGTGAACTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  >  2:111268/1‑143 (MQ=255)
       gatggTAACCCGCTGTTCTTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCct                                                                                                                                <  1:293495/140‑1 (MQ=255)
                     gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTGTCGGTGACGCGGTGCTCTGCATTTCACCGCGATTGCTCTGCAGGCTCTGCGTCGCCTCTTCTACTTTcccc                                                                                                                  >  2:273068/1‑141 (MQ=255)
                         tttgttGCTGGCGCGGTCGAATTGGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                              <  1:32187/143‑1 (MQ=255)
                                     gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCTACGGTTCGCCGTACTcca                                                                                                  >  1:42577/1‑143 (MQ=255)
                                                   cTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAgg                                                                                    <  2:435298/143‑1 (MQ=255)
                                                    tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCAGGa                                                                                   >  1:385540/1‑143 (MQ=255)
                                                           ttGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                            <  2:468594/143‑1 (MQ=255)
                                                               ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGtca                                                                        >  1:681602/1‑141 (MQ=255)
                                                                    aaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCtg                                                                   >  2:671088/1‑143 (MQ=255)
                                                                    aaGTGGAAGTACCCGCAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCCGGCAGATGACGTAATCCtg                                                                   >  1:602982/1‑143 (MQ=255)
                                                                     aGTGGAAGTCCCCGGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                  <  2:214362/143‑1 (MQ=255)
                                                                          aaGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc                                                             <  2:221053/143‑1 (MQ=255)
                                                                           aGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCAACGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCATACTCAACCACCTTCCGCAGGTAGATGACGttctcccttgctgct                                                            >  2:188969/1‑129 (MQ=255)
                                                                                    gggattgcgtCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                   <  2:12034/134‑1 (MQ=255)
                                                                                               ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGGAGGCACTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTGCCTCTTTCACTTCTGTATCGaacaa                                        >  2:619292/1‑143 (MQ=255)
                                                                                                gTGACGTGGTGCTCTGCATTTCACCGAGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                       <  1:596113/143‑1 (MQ=255)
                                                                                                            tCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCCCAGGAAGCTGTCGTAATCCTGTGCTGCTTTCAGTTCGCTCTCGAACAAACACTCTTTgccc                           >  2:342279/1‑140 (MQ=255)
                                                                                                                   ttCACCGTGATTGCTACGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAGCGAACAAACACTCTTTGAAACGCCATg                    <  1:289210/143‑1 (MQ=255)
                                                                                                                   ttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTGCTCCGCCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATg                    <  1:566309/143‑1 (MQ=255)
                                                                                                                      aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCCGGACGATGTCGTCCTCCTGTGCCGCTTTCAGTTAGGTATAGAACAAACACTCTTTAAAAAGCCATGcac                 >  2:148184/1‑141 (MQ=255)
                                                                                                                                  atGCAGGCTCTGCGTCGCCTCTTCTACTTCCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt     <  2:151559/143‑1 (MQ=255)
                                                                                                                                    gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAACCGCAGGAAGATGTCGTACTCCTGTGCCGCTTTCAGTTCGGTATCGACCAAACACTCTTTGAAACGCCATGCCCAGCTAAAGACCCgg   >  2:22443/1‑143 (MQ=255)
                                                                                                                                     cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGtctttacctggg  <  2:415126/143‑12 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132650‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: