Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,316,679	A→G	T169A (ACC→GCC)	rcsB →	response regulator in two‑component regulatory system with RcsC and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,316,679	0	A	G	100.0%	188.5 / NA	65	T169A (ACC→GCC)	rcsB	response regulator in two‑component regulatory system with RcsC and YojN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/36);  total (29/36)

CAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATA  >  NC_000913/2316544‑2316817                                                                                                                                        |                                                                                                                                           cAGAAAGGGAAGAAATTTACCCCGGAAAGCGGTGCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGAt                                                                                                                                     <  2:229876/143‑1 (MQ=255)  aGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATc                                                                                                                                    >  1:127513/1‑143 (MQ=255)   gAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCg                                                                                                                                   <  1:561185/143‑1 (MQ=255)         gaagaaATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTaaaa                                                                                                                             >  2:251437/1‑143 (MQ=255)          aagaaATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGCAGGCTTCCTGGTGGCCGAGATCGCTaaaaa                                                                                                                            >  2:542418/1‑143 (MQ=255)           agaaATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAACGAGCGTGCAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAg                                                                                                                           >  2:13825/1‑143 (MQ=255)                   aCCCCGGAGAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCg                                                                                                                   <  2:622284/143‑1 (MQ=255)                    tcccGGAAAGTGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGc                                                                                                                  <  2:17824/142‑1 (MQ=255)                      ccGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAACGCTTCCTGGTGGCTGAGCTCGCTAAAAAGCTGAACCGccg                                                                                                                >  1:517394/1‑141 (MQ=255)                         gAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTAt                                                                                                             >  2:311311/1‑143 (MQ=255)                           aaGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGACGAGATCGCTAAAAAGCTGAACCGCAGTATTa                                                                                                           >  1:570567/1‑143 (MQ=255)                              cGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGACGGCTTCCTGGTGGCCGATATCGCTTAAAAGCTGAACCGCATCATTaaaa                                                                                                        >  2:108351/1‑143 (MQ=255)                              cGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATCaaaa                                                                                                        >  1:708930/1‑143 (MQ=255)                                   ctcGCCTGGTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATc                                                                                                   <  2:661504/143‑1 (MQ=255)                                    tcGCCTGTTGGGAAAAATCAGGGCGGGTGTTTGTGGTGGGAACCCCCACACGCCAAAAGAGGGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCa                                                                                                  <  1:504712/143‑1 (MQ=255)                                     cGCCTGTTGGAAAGAAGCAGTGCTGGTGGTTACGGTGACAAGCGTCGCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGTAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAg                                                                                                 <  2:58423/143‑1 (MQ=255)                                         tGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGc                                                                                             <  2:416254/143‑1 (MQ=255)                                         tGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGc                                                                                             <  2:295967/143‑1 (MQ=255)                                             ggAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAACACCATCAGTAGCCaga                                                                                         >  2:181051/1‑143 (MQ=255)                                               aaaaaaTCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTAGTAAAACCATCAGTAGCCagaag                                                                                       >  1:480631/1‑143 (MQ=255)                                                aaaaaTCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCagaaga                                                                                      >  2:477575/1‑143 (MQ=255)                                                          gCTGGTGGTTACGGTGACCAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCgat                                                                            <  2:713226/143‑1 (MQ=255)                                                           cTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCgatg                                                                           >  1:521048/1‑143 (MQ=255)                                                           cTGGTGGTGACGGTGACAACCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCgatg                                                                           <  2:221957/143‑1 (MQ=255)                                                             gggggtTACGGTGGCAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCgatgat                                                                         <  1:386340/140‑1 (MQ=255)                                                                ggtTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGAtgatgat                                                                      >  1:420995/1‑142 (MQ=255)                                                                   taaggTGAAAAGCGTCTCTCGGCAAAAGAGAGGGAAGTACTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCt                                                                   <  2:40168/140‑1 (MQ=255)                                                                     cGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTACAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTgg                                                                 >  2:56530/1‑143 (MQ=255)                                                                       gTGACAAGCGTCTCTCGCCAAAATAGAGTGAACTTCTGCGCCTGGTTGGGGAAAGCGTCCTGGTTGCCGAGATCGCTAAAAAGCTGAACCGCATTATTAAAACCATCAGTAGCCAGAAGAAATCTGCTATGATGAAGCTGGgt                                                               <  1:44129/143‑1 (MQ=255)                                                                          aCAGGCGTCTCCCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTc                                                            <  2:397055/143‑1 (MQ=255)                                                                            aggCGTCTCTCTCCAAAAGAGAGTGAAGTGCTGCGCCTGTTTGCGGAAGGCGTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCga                                                          <  2:481540/141‑1 (MQ=255)                                                                            aagccttgcgcggcAAAAGATAGTGAAGTTCTGCGCCTGTTTGCCGTAGGCTTCCTGGTGGCCGAGATAGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCga                                                          <  1:493509/130‑1 (MQ=255)                                                                            aaGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGCGGAAATCTGCGATGATGAAGCTGGGTGTCga                                                          >  2:89646/1‑143 (MQ=255)                                                                              gCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCgagc                                                        >  2:531717/1‑142 (MQ=255)                                                                                 gctcacGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACg                                                     <  1:583996/142‑1 (MQ=255)                                                                                    ctcGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAGAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAAAGata                                                  >  1:393422/1‑143 (MQ=255)                                                                                           aaaGAGCGTGAAGCTCTGCGCCTGTCTGCGGCAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGACGAAGCTGGGTGTCGAGAACGATATCGCCct                                           <  2:190094/143‑1 (MQ=255)                                                                                           aaaGAGAGTGAAGTTCTGCGCCTGTGTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCct                                           <  2:110262/143‑1 (MQ=255)                                                                                            cagagagTGAAGTTCGGCGCCTGTGTGCGGCAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTACAACCATCAGTAGCCAGAATATATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCctg                                          <  2:82024/142‑1 (MQ=255)                                                                                              gagagTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAACGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCccgct                                        >  1:346709/1‑143 (MQ=255)                                                                                                   tGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAGtt                                   >  2:672975/1‑143 (MQ=255)                                                                                                   tGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGAGGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAAtt                                   >  2:301710/1‑143 (MQ=255)                                                                                                     aaGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTAt                                 <  1:251437/143‑1 (MQ=255)                                                                                                      aGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTAtc                                <  2:415228/143‑1 (MQ=255)                                                                                                      aGTGCTGCGCCTGGTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTAtc                                <  2:657204/143‑1 (MQ=255)                                                                                                              ggcTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCa                        <  2:480631/141‑1 (MQ=255)                                                                                                              gcCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAACCAGAAGAAATCTGCGAAGATGAAACTGGGTGAAGAGAAAGATATCGAAATGCGGAATTAtgtctataca                        >  1:3906/1‑138 (MQ=255)                                                                                                                cTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGCAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGt                      <  2:527062/143‑1 (MQ=255)                                                                                                                cTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGt                      >  1:385132/1‑143 (MQ=255)                                                                                                                      gCGGAGTGCCTCCTGGTTGCCGAGATCGCTAAAACGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCtt                <  1:114097/143‑1 (MQ=255)                                                                                                                      gCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCCTCAGTGAccct                >  2:495728/1‑141 (MQ=255)                                                                                                                        ggAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCCCAGTTAACCCTCTTCAGTGACCTTaa              >  1:340783/1‑143 (MQ=255)                                                                                                                        ggAAGACTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGCATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGATGTCGAGAACGATAACGCGCTGCTGAATGAGAAATCTTCAATGACGTTaa              >  1:25195/1‑143 (MQ=255)                                                                                                                         gAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGATGAATAACACATATAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTCTCGAGAACGATATCGCCCTGCCGCATGATCTCTGTTCAGTGACCTTact             >  1:510753/1‑141 (MQ=255)                                                                                                                           aGGCGTCATGGTGGCCGAGGGCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGAGATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTc           <  2:170262/143‑1 (MQ=255)                                                                                                                             gCTTCCTGGTGGACGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCg         <  2:72179/143‑1 (MQ=255)                                                                                                                              tttCCTGGTGGCCGGGATAGGTAAAAAGCTTGACCGCAGTAATAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGTTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCgg        <  1:92746/142‑1 (MQ=255)                                                                                                                              cTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCgg        <  2:328121/143‑1 (MQ=255)                                                                                                                               ttCCTGGTGGCCTAGATCGCTAAAAAGCTGAACCGCAGTATTAAATCCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc       <  1:328774/143‑1 (MQ=255)                                                                                                                               ttCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTCAGTCCGGc       <  1:477575/143‑1 (MQ=255)                                                                                                                               ttCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc       <  1:569832/143‑1 (MQ=255)                                                                                                                               ttCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc       <  2:702880/143‑1 (MQ=255)                                                                                                                                 ccTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAg     >  1:193368/1‑143 (MQ=255)                                                                                                                                  ggggTGGCCGAGATCGCTAAAAAGATAAAAAGCATTATTAATTCCTGCAGGAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGa    <  1:343814/141‑1 (MQ=255)                                                                                                                                    ggTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCGATGTAGCAGGATCTCTCTTCAGCGACCTTAAGTCAGGCAGATa  >  1:694695/1‑143 (MQ=255)                                                                                                                                        |                                                                                                                                           CAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATA  >  NC_000913/2316544‑2316817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑