| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,527,589 | C→T | Q375* (CAG→TAG) | yrfF → | putative RcsCDB‑response attenuator, inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,527,589 | 0 | C | T | 97.9% | 127.3 / NA | 49 | Q375* (CAG→TAG) | yrfF | putative RcsCDB‑response attenuator, inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (23/24); minor base G (0/1); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
AAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTT > NC_000913/3527449‑3527726 | aaGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGACTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAg < 1:79688/143‑1 (MQ=255) aacaCTTTCCGTTACAGCACTGTCTGCTCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCAtt < 1:259284/140‑1 (MQ=255) gagcTTTCCGGGACAGCACTGGCGGCGCAGTACGATTATCGCTGCGGGCTCGC‑TGATGGTGCTGTTTATGCTGGTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTg < 2:406901/140‑1 (MQ=255) aagcTTTCCGTTGCGGCACGGGGTGCGCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTg < 1:554275/140‑1 (MQ=255) aaaCTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTGATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCACTCATCGATGAAAGGCGCGTAGACCATTg > 1:482771/1‑143 (MQ=255) actgtgcgctACAGCACTGTCAGCGCAGTACGATTATCGCTGCGAGC‑CGCAGGCGTTTCCTGTTACTGCGCTTATGCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGaa < 1:150646/134‑1 (MQ=255) cTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCAGTTATTCTGGGTCCCGCTGGATATGCCGCAGCAATCCACTCACTCATGGATGAAAGGCGAGTAGACCACTGAAg > 1:278177/1‑143 (MQ=255) ccGTTAGAGCACTGGCTGCGCAGTACGATTACCGCTGCGGGCTCGC‑TGCGGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCCCTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGccac < 2:677567/143‑1 (MQ=255) ccGTTACTGCCCTGTCTTCACAGTACGATTATCGCGGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGccac < 1:424932/143‑1 (MQ=255) cACTGGCTGCGCAGTACGCTTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTaaa < 1:20522/143‑1 (MQ=255) tGGCTGCGCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCCATGAAGCCCCCAACGTAAaaca > 1:602614/1‑143 (MQ=255) tGGCTGCGCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAaaca < 2:281308/143‑1 (MQ=255) tGGCTGCGCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCACGCTGGATATGCCGCTGCAATTCGCTCTCTCATGGGTGAAGGGCGCGTAGACCATTGAAGCCGCCAGGGTAAaaca > 2:341517/1‑143 (MQ=255) ggcggcGCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCGACCAGCGTAAaacaa < 1:605066/143‑1 (MQ=255) gCTGCGCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAaacaac < 1:712078/143‑1 (MQ=255) ctccgcAGTTCGATTCTCGCTTCGGGCTCGC‑TACTGGTTCTGTTTATGCTGTTATTCTGGATCCCGCTGAATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACt < 1:487992/140‑1 (MQ=255) gactATCGCTGCGGGCTCGC‑CGCTGGTGCTGTGTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTg < 2:174328/140‑1 (MQ=255) gATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATCTCAGCCACCAGCGTAAAACAACCGGCTGAAGCTg > 1:415312/1‑143 (MQ=255) cTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGAGCCCGCTGGATATGCCGCTGGAGTTCACTCTCTCATGGATGAAAGGCGCGTAGACCGTTGAAGCGAGCAGCGGAAGACACCGGGGTGGCGGTGGCGTGAgg > 1:2186/1‑143 (MQ=255) gcgcgcTCGC‑GGCTTCTGCTGTTTATGCTGTTAGTCTGGATCCCGCTGGATATGCCGCTGAAATTCACGCCCTCCGGGATGCAACGCGCGTCGACCATTGAAGCCGCCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGt < 1:38253/143‑1 (MQ=255) ggttgccggctgGTGCGGCGTATGCTGGTATTCGGGATCCCGGTGGATATGCCGCTGAAATTCACGCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAgg < 1:540926/135‑1 (MQ=255) gCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTTTCATCGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGGCAAACAACTGGCTGTCGCTGCCGTGCGGCTCGGc > 2:66056/1‑143 (MQ=255) cTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAACGGCGCGTAGACCATTGAAGCAACCAGAGAAAAACAACTGGATAACGCTGGCGTGTGGGTAGGCg > 2:228013/1‑143 (MQ=255) cTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTCAACCATTGAAGCCCCCAGCGTACCACAACTGGCTGACGCTGGCGTGCGGGCAGGcc > 1:568174/1‑142 (MQ=255) tCGC‑GGCTGGTGCTGTTTATGCTGTTATTCTGGCTCCCGCTGTATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGa < 1:43554/143‑1 (MQ=255) gTGCTGTTTATGCTGTTATACTGGAGCGCACTGGGTTTGGCTCTCAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑Cg < 2:518530/143‑1 (MQ=255) tGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGt > 1:149696/1‑143 (MQ=255) gCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTa > 2:691716/1‑143 (MQ=255) gTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAATGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTAGCTGAAGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTa > 2:10711/1‑143 (MQ=255) tttATGCTGTTATTCTAGATCCCGCTGGAGATGCCGCTGAAATTCACTCTCTCATGGATGAACGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑Cggcgtag > 2:690442/1‑137 (MQ=255) acgCTGTTACTCTGGATCCCGCTCGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGCCCCTCGACGCCACTAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCgg < 2:268699/141‑1 (MQ=255) tGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGGACg > 2:33341/1‑143 (MQ=255) gTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACgg < 2:87698/143‑1 (MQ=255) gTTATTCTGGACCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCCTTGAAGCCACCAGCGTAAAACAACTGGCGGACGCTGGCGTGCGGGAAGGCGATAGACTGCCGTA‑TAGCGGTACgg > 1:248837/1‑143 (MQ=255) cTGGATACCGTTGGATATGCCGCTGAAATTCACGCTCTCGTGGCTGGAAGGCGCAGAGACGGTTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAAtgt < 2:300536/143‑1 (MQ=255) tGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAAtgtg > 2:154119/1‑143 (MQ=255) ggacgCCGCTGGTATTCACTCTCTCGTGGATGCGGGGCGGGTGGCCCATTGAAGCCACCAGCGTAAGACAACTGGCTGGGGCGGGTTTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACt < 1:471713/139‑1 (MQ=255) cGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAAATGGCTGACGCTGGCGCGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATACGAACTTCCGGa > 1:307215/1‑143 (MQ=255) ttCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTCAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGCACTTCCGGAACCTGGAg > 1:267063/1‑143 (MQ=255) tCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAgc > 1:673149/1‑143 (MQ=255) tCACTCCCTAATGGATTAAAGGCGCGTAGCCCATGGAGGCCACCAGCGTAAAACAACTGGCTGACGCGGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAgc < 1:113735/143‑1 (MQ=255) ctctctCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAACATTCGAAGTTCCGCAACCTGGAGcgcg > 2:697296/1‑143 (MQ=255) tctctcATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAAGGTGAAATATTAGAACTTCCGGAACCAAGAgcgcaa > 1:50963/1‑141 (MQ=255) ctctcATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGaa < 2:369434/143‑1 (MQ=255) ccaaTGGCTGAACGGCGCGTAGGCCATTGGCGCCACCAGCGTAAAACAACTGGCGTACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATGAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGaaaa < 1:29401/140‑1 (MQ=255) gATGAAGGGCGCGTAGCGCATTGAAGCCACCAGCGTACAGGAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAAtt < 2:227471/143‑1 (MQ=255) catacacggCGCGCAGCCCATGAAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAAtt < 1:610751/136‑1 (MQ=255) aTGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAGTATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTc > 2:447239/1‑143 (MQ=255) gAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑AGCATTAGTCCCACTTGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCACTTccc > 2:163435/1‑141 (MQ=255) gcgcgtAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCtttt < 2:149696/143‑1 (MQ=255) | AAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGC‑TGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTG‑CGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTT > NC_000913/3527449‑3527726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |