Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,705,863:2 +T 4.1% coding (1260/1800 nt) lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,705,8632.T 4.1% 389.8 / 11.9 97coding (1260/1800 nt)lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base . (46/47);  new base T (2/2);  total (48/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01

TTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAA  >  NC_000913/2705576‑2706150
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                               
ttCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTACGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑g                                                                                                                                                                                                                                                                                                >  1:349277/1‑289 (MQ=255)
        ctcCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACg                                                                                                                                                                                                                                                                                        >  1:667944/1‑289 (MQ=255)
        ctcCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACg                                                                                                                                                                                                                                                                                        >  1:362115/1‑289 (MQ=255)
           caccaACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGtt                                                                                                                                                                                                                                                                                      >  1:52349/1‑288 (MQ=255)
             ccaACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGcc                                                                                                                                                                                                                                                                                   >  2:183873/1‑289 (MQ=255)
             ccaACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGcc                                                                                                                                                                                                                                                                                   >  1:530012/1‑289 (MQ=255)
                           cgGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGGCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCAACGGGATCTCGTACGTCAGCGCCCCCTGATTACCGTTGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATCTGCCTGCg                                                                                                                                                                                                                                                                     >  2:438010/1‑289 (MQ=255)
                               tttGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGgcag                                                                                                                                                                                                                                                                 <  2:95561/289‑1 (MQ=255)
                                 tGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGgcagca                                                                                                                                                                                                                                                               <  2:78240/289‑1 (MQ=255)
                                 tGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGgcagca                                                                                                                                                                                                                                                               <  1:643039/289‑1 (MQ=255)
                                           cccagtgggtgATCGACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGaca                                                                                                                                                                                                                                                     <  1:28954/285‑1 (MQ=255)
                                                  ggtgATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTCTCTACGCACAAC‑‑GTAATAACGTTGCCGAGCTATGCCTGCGGCAGCAGCATGTGACACCCTGCa                                                                                                                                                                                                                                              >  2:464534/1‑289 (MQ=255)
                                                  ggtgATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCAACCTGATTACCGTGGTAAACAATATTGGTCTGCACGCCGCGTCTTTCTACCCACAAC‑‑GTAATAACGTTGCCGAGATATGCCGGCGGCCACAGCCTGCGacacctcgac                                                                                                                                                                                                                                              >  2:578146/1‑283 (MQ=255)
                                                    tgATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCaa                                                                                                                                                                                                                                             <  1:10232/288‑1 (MQ=255)
                                                    tgATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCaa                                                                                                                                                                                                                                             <  2:626971/288‑1 (MQ=255)
                                                            gCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATACAGTTGCCGATATATGCCTGCGGCAGCAGCCTGTGACACTCTGCAATCGGCtac                                                                                                                                                                                                                                     >  2:701979/1‑286 (MQ=255)
                                                              aaagggtgccacccGTTCGCCGGTTGTTAATACGTCTACAAGTACCATGTAGGACACCTGAGAGCGTTTGAAGTTGTTACCCAGTGTCGACTAACAACGCGTGGTAGATGTCAGGCGCTGTAAGAAGTCGAAGAGCACTTCCGCCATCGGGAACTGGTACGTAAGCGCCACCTGATTACCGTGGTAAGCCATTTTGGTCTGCACGCCGCATTGTTCTGCGCACAAC‑‑GTAATAACATTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTcgct                                                                                                                                                                                                                                   <  2:669371/275‑2 (MQ=255)
                                                                gcgcATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAg                                                                                                                                                                                                                                >  1:135832/1‑289 (MQ=255)
                                                                 cgcATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGac                                                                                                                                                                                                                                                      >  2:649790/1‑266 (MQ=255)
                                                                 cgcATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGac                                                                                                                                                                                                                                                      <  1:649790/266‑1 (MQ=255)
                                                                 cgcATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTCCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGt                                                                                                                                                                                                                               >  2:91747/1‑289 (MQ=255)
                                                                     tcacgacGTTCACCGTTGATTACTACGTCTACACGTACCATGTCTGACGCCTGGAAGCGCTTGAAGTTGTAAGCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGt                                                                                                                                                                                                                           <  2:433082/284‑1 (MQ=255)
                                                                     tCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGt                                                                                                                                                                                                                           <  1:183873/289‑1 (MQ=255)
                                                                          acGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATg                                                                                                                                                                                                                      <  1:146835/289‑1 (MQ=255)
                                                                            gTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGgcagc                                                                                                                                                                                                                                                                <  1:264365/245‑1 (MQ=255)
                                                                            gTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGgcagc                                                                                                                                                                                                                                                                >  2:264365/1‑245 (MQ=255)
                                                                              tCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTAt                                                                                                                                                                                                                  >  1:700042/1‑289 (MQ=255)
                                                                                    ttGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTAcc                                                                                                                                                                                                             >  1:168870/1‑288 (MQ=255)
                                                                                           aTACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCa                                                                                                                                                                                                                                 <  2:520204/261‑1 (MQ=255)
                                                                                           aTACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCa                                                                                                                                                                                                                                 >  1:520204/1‑261 (MQ=255)
                                                                                            tACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGc                                                                                                                                                                                                                                                                                    >  1:393375/1‑209 (MQ=255)
                                                                                            tACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGc                                                                                                                                                                                                                                                                                    <  2:393375/209‑1 (MQ=255)
                                                                                                 cTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCTAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCCCAGTTCGTTAATGTTATTTACCGCAGGCATCTTGGa                                                                                                                                                                                               >  1:319049/1‑289 (MQ=255)
                                                                                                  tACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCACCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGCTGTTCTTTACCGCAAGCTGCTTg                                                                                                                                                                                                 >  1:377650/1‑286 (MQ=255)
                                                                                                     acGTACCATGTCGGACGGCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgcaggcatatctcggcaa                                                                                                                                                                                           <  1:712526/289‑12 (MQ=255)
                                                                                                             tGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGAc                                                                                                                                                                                   >  1:474067/1‑289 (MQ=255)
                                                                                                              gTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCg                                                                                                                                                                                     >  2:249747/1‑288 (MQ=255)
                                                                                                              gTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCg                                                                                                                                                                                     >  2:16830/1‑288 (MQ=255)
                                                                                                              gTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACg                                                                                                                                                                                  >  1:426799/1‑289 (MQ=255)
                                                                                                               tCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATAGAAGAAGTCGAGCACCACTTCCGCCTTCGGGATCTCGTACGTCAGCGCCACCTGAATACCGTGGTAAACCATATTGGCTTGCACGCCGTGTTTTTCTACGCACAAC‑‑GTAATAATGTTGCCGAGATACTCCTGCGGCAGCTGGTACTGACACTCCTCAATCTGTTCGCGCAGGTAGGTGTTGTTATTTACCgctggtctactggatgggacttccgctt                                                                                                                                                                                 >  2:100495/1‑266 (MQ=255)
                                                                                                                 ggACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTcgcg                                                                                                                                                                                                                                   <  1:506518/237‑1 (MQ=255)
                                                                                                                 ggACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTcgcg                                                                                                                                                                                                                                   >  2:506518/1‑237 (MQ=255)
                                                                                                                  gACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGa                                                                                                                                                                              >  2:679519/1‑289 (MQ=255)
                                                                                                                     gCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATaa                                                                                                                                                                           <  1:27477/289‑1 (MQ=255)
                                                                                                                      ccTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATa                                                                                                                                                                            >  1:19295/1‑287 (MQ=255)
                                                                                                                       ctgataGCGCTTGAAGTTGTAAACCAGAGACGCATAACCACGCGGGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCGTCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTAACACTCTGCAATCGGTTCGCGCAGTTCGTAGATGATATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAAc                                                                                                                                                                          <  1:100495/283‑1 (MQ=255)
                                                                                                                         ggAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACt                                                                                                                                                                         >  1:134116/1‑287 (MQ=255)
                                                                                                                                        tGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGttt                                                                                                                                                         <  2:52349/288‑1 (MQ=255)
                                                                                                                                            caccAGAGACGCATAACCACGCGAGGTGGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTGCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACt                                                                                                                                                    <  1:372763/287‑1 (MQ=255)
                                                                                                                                              ccAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACt                                                                                                                                                    <  1:16830/289‑1 (MQ=255)
                                                                                                                                              ccAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACt                                                                                                                                                    <  1:249747/289‑1 (MQ=255)
                                                                                                                                               cAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCa                                                                                                                                                 <  1:679519/289‑1 (MQ=255)
                                                                                                                                                          aaCCACGCGAGGTAGAATTCAGGCGAGCGAAGAAATCGGGGACCACTTCCGCCTTCGGGATATCGTACGTCAACGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTATGCAATCGGCTCGCGCAGTTCGTAGATATTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACAGt                                                                                                                                      <  2:139309/289‑1 (MQ=255)
                                                                                                                                                               ggggagttggATTTCGGTCGAACTAAGAAGACAAGAACCCATGCCGCCATCGGGATCTACGACGTCAGCACCACCTGCTGACGTTGGTAAACCATAGTGCTCTGCACGCCGCGTGTTTATACGCACACC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGTCTCGCGCAGTTCATAGATGTTATTTACCGCGGGCAGCTTGGATGGGCTGTCGGCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGgcg                                                                                                                                 <  2:571663/280‑1 (MQ=255)
                                                                                                                                                               cgcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACt                                                                                                                                           >  2:358196/1‑279 (MQ=255)
                                                                                                                                                               cgcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACt                                                                                                                                           <  1:358196/279‑1 (MQ=255)
                                                                                                                                                                gcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGgcgc                                                                                                                                >  1:475589/1‑289 (MQ=255)
                                                                                                                                                                 cgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATg                                                                                                                                                                                                                      >  1:348103/1‑202 (MQ=255)
                                                                                                                                                                 cgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATg                                                                                                                                                                                                                      <  2:348103/202‑1 (MQ=255)
                                                                                                                                                                  gAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCa                                                                                                                               <  2:168870/288‑1 (MQ=255)
                                                                                                                                                                          tttCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                                       <  1:578146/288‑1 (MQ=255)
                                                                                                                                                                          tttCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                                       <  1:533281/288‑1 (MQ=255)
                                                                                                                                                                          tttCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                                       <  1:91747/288‑1 (MQ=255)
                                                                                                                                                                              aGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCa                                                                                                                                                 <  2:333382/258‑1 (MQ=255)
                                                                                                                                                                              aGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCa                                                                                                                                                 >  1:333382/1‑258 (MQ=255)
                                                                                                                                                                                            atcgtacaccccTTCCGCCCACGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCa                                                                                                    <  2:426799/283‑1 (MQ=255)
                                                                                                                                                                                              cGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCa                                                                                                    <  1:701979/287‑1 (MQ=255)
                                                                                                                                                                                                    ccacTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAg                                                                                            <  2:135832/289‑1 (MQ=255)
                                                                                                                                                                                                     cacTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa                                                                                           <  2:700042/289‑1 (MQ=255)
                                                                                                                                                                                                       cTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgca                                                                                                                                                                                                          <  2:701052/176‑1 (MQ=255)
                                                                                                                                                                                                       cTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgca                                                                                                                                                                                                          >  1:701052/1‑176 (MQ=255)
                                                                                                                                                                                                           cGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCTTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTcc                                                                                     <  1:152043/289‑1 (MQ=255)
                                                                                                                                                                                                           cGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGtt                                                                                       >  1:295331/1‑287 (MQ=255)
                                                                                                                                                                                                           cGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTcc                                                                                     <  2:295331/289‑1 (MQ=255)
                                                                                                                                                                                                             ccATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTg                                                                                   >  1:113330/1‑289 (MQ=255)
                                                                                                                                                                                                               aTCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTCCCGCAGGCAGCTTGGATGGGCTGTGGACGTAGATAAATTCTCTTGACGTGGTTTCAACTTCATCACCTACGGTCCGCGCAGTTGTGATCAGATCCAGATCTTTTTTCCGTTCCAGACGTTCCTg                                                                                   >  2:687879/1‑287 (MQ=255)
                                                                                                                                                                                                                tCGGGATCTCGTACGGCAGCGTCACCTGATTACCGTGGTAAACCAACTTGGTCTTCACGCCGCGTTTTTTTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgat                                                                                <  2:349277/289‑1 (MQ=255)
                                                                                                                                                                                                                              gTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgca                                                                  >  1:405575/1‑289 (MQ=255)
                                                                                                                                                                                                                                  gcgcCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGgcag                                                                                                                                                                                                                                                                 >  1:553381/1‑94 (MQ=255)
                                                                                                                                                                                                                                  gcgcCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGgcag                                                                                                                                                                                                                                                                 <  2:553381/94‑1 (MQ=255)
                                                                                                                                                                                                                                            aTTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                                       >  1:432952/1‑222 (MQ=255)
                                                                                                                                                                                                                                            aTTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                                       <  2:432952/222‑1 (MQ=255)
                                                                                                                                                                                                                                               ttctcggTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCg                                                 <  2:604844/284‑1 (MQ=255)
                                                                                                                                                                                                                                                   tGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAgtg                                                                                                                            >  2:562383/1‑210 (MQ=255)
                                                                                                                                                                                                                                                   tGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAgtg                                                                                                                            <  1:562383/210‑1 (MQ=255)
                                                                                                                                                                                                                                                      tAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGa                                          >  1:81964/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              atTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCa                                                                                                    >  1:707195/1‑223 (MQ=255)
                                                                                                                                                                                                                                                              atTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCa                                                                                                    <  2:707195/223‑1 (MQ=255)
                                                                                                                                                                                                                                                               ttGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAg                                  >  2:558297/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                tGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCTGAGGAAGCCGCAGCg                                            >  2:613121/1‑277 (MQ=255)
                                                                                                                                                                                                                                                                tGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCTGAGGAAGCCGCAGCg                                            <  1:613121/277‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGccc                               >  2:716832/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                     tGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgcagg                                                                                                                                                                                                        <  2:426429/116‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgcagg                                                                                                                                                                                                        >  1:426429/1‑116 (MQ=255)
                                                                                                                                                                                                                                                                     tGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgc                            >  1:1778/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                      tACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGc          <  1:716832/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        cGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCCCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCCACTTCATACACTACGGTCGGCGCAGTCGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGACCTCCCTGTGCAGCAGGCCGACGAAGCCGCCGCGGTAACCAACGCACAGCCCGCTGGCGCCTTCGGGCt         >  2:653292/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                              aC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGaa  <  2:362115/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                               
TTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAAC‑‑GTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAA  >  NC_000913/2705576‑2706150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: