Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 124.0 / NA 45I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/20);  total (25/20)

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132647‑2132926
                                                                                                                                            |                                                                                                                                           
ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc                                                                                                                                           >  2:621585/1‑143 (MQ=255)
     tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAACAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGCGATTGCTACGCAGGCTCTGCGt                                                                                                                                      >  2:76054/1‑143 (MQ=255)
     tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGACTTATTACGTCGACGTGTTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt                                                                                                                                      >  2:446827/1‑143 (MQ=255)
      gggagagtgttgagaGCTGTTATTGTGGGCTGGCGCTGTCGACGTGGTGTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                     <  1:251000/129‑1 (MQ=255)
        gagagtgtagccggCTGAGTTTGTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                   <  1:199092/130‑1 (MQ=255)
              gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCtcttc                                                                                                                             >  2:706316/1‑143 (MQ=255)
               tAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATCTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct                                                                                                                            <  2:931688/143‑1 (MQ=255)
                aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGGCGCCCTTCTAc                                                                                                                          >  2:725962/1‑143 (MQ=255)
                            ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                               >  2:695753/1‑143 (MQ=255)
                             tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTTCGTCGCCTCTTCTGCTTTCCACGGTTc                                                                                                              >  1:297958/1‑143 (MQ=255)
                             tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTAGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCGCTCTCTGCTTCGCCTCTTCCACtctgtctcgtga                                                                                                              >  1:308849/1‑132 (MQ=255)
                                      gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGACATGCAGGCTCTGCGTCGCCTCGTCTACTTTCCACGGGTCGCCGTCCTc                                                                                                     >  1:311132/1‑143 (MQ=255)
                                          ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                                 <  1:719740/143‑1 (MQ=255)
                                           gtcgcgtttGTCTGTGTGCTTGCGGTATAAGTGGATGTACGCGGAGAATTTTTTCGGTGACGATGTGATCTGCCTTTCTCCGTGATCGATACGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTccacca                                                                                                <  1:477031/137‑1 (MQ=255)
                                           gTCGAAATTGGCAGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAAATTTTCCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                                <  2:430492/143‑1 (MQ=255)
                                                   tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTACTCTGCATTGCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc                                                                                        <  2:776392/143‑1 (MQ=255)
                                                    gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa                                                                                       >  1:887085/1‑143 (MQ=255)
                                                    gTCTTTGTGCCTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGCCCACAACGTTTTTGTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa                                                                                       <  2:490314/143‑1 (MQ=255)
                                                       tttGTGCTTGCGGTAAAAGTGAAAGAACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAggc                                                                                    >  1:931761/1‑142 (MQ=255)
                                                         tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg                                                                                  <  2:956303/143‑1 (MQ=255)
                                                          gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCCGGAAGa                                                                                 >  2:38615/1‑143 (MQ=255)
                                                                      aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCt                                                                     <  1:446827/143‑1 (MQ=255)
                                                                         gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTGCTCCtgtg                                                                  >  2:651973/1‑143 (MQ=255)
                                                                         gTGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                  <  2:755292/143‑1 (MQ=255)
                                                                          ggggagtggccGGAGAATTTGTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGc                                                                 <  1:33186/134‑1 (MQ=255)
                                                                            gAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTTCCg                                                               >  1:538018/1‑143 (MQ=255)
                                                                             aaGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGACGTCGTAAACCTGTGcccc                                                              >  1:1008097/1‑141 (MQ=255)
                                                                               gTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTGTTCTACTTTCCACGGTTCGCCGTACTCACATGCCAGCCGCAGGAAGCTGGCGTCATCCTGTGCCGCtc                                                            >  1:171347/1‑142 (MQ=255)
                                                                                  cccGGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                         <  1:205938/143‑1 (MQ=255)
                                                                                   ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCCTCCGCAGGAGGATGTCGTCATCCTGTGCCGCTTTCAg                                                        >  2:185317/1‑143 (MQ=255)
                                                                                    cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGACGTCGTGATTCTGTGGCGGTTTGAGt                                                       >  2:483242/1‑143 (MQ=255)
                                                                                     ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                      <  2:674552/143‑1 (MQ=255)
                                                                                       agaATTTTTTCGGTGACGAGGTGGTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                    <  1:621619/143‑1 (MQ=255)
                                                                                            tttgtgggTGCCGAGGTGATCTTCATTTCACCGTGATTTATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                               <  1:888441/137‑1 (MQ=255)
                                                                                                  ggggACGGGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa                                         <  1:443359/140‑1 (MQ=255)
                                                                                                   gTGCCGCGGTGGTCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCGACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                        <  1:391838/143‑1 (MQ=255)
                                                                                                        gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActc                                   >  2:1057591/1‑143 (MQ=255)
                                                                                                           gTGATCGGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCTAACAAACACTCttt                                <  1:54141/143‑1 (MQ=255)
                                                                                                             gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGCTCGATATCGAACAAACACTATTTGa                              >  1:711907/1‑143 (MQ=255)
                                                                                                              aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTACTCCACCACCATCCGCACGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGCACAAACACTCTTTGaa                             >  1:299034/1‑143 (MQ=255)
                                                                                                                 tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACGCTCTTTGAAACg                          >  2:295544/1‑143 (MQ=255)
                                                                                                                         aCCGTGTTTGATATGCAGGCTCTGCTTCGCCGCTTCTACTTTCCACGGTTCGCCGTACTTCACCGCCAGCCGCAGGAAGATGTCGTAATCCTGTGATGATTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                  <  1:76054/143‑1 (MQ=255)
                                                                                                                         aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCCTTGCCACGCCacgggc                  >  1:363019/1‑138 (MQ=255)
                                                                                                                                        cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTGCCGCTTTCCGTTCGGTATCGAACACACACTCTTTGAAACGCCATGCCCCGGTCAACACCtcgc   >  1:187378/1‑140 (MQ=255)
                                                                                                                                         aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt  >  2:687901/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132647‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: