| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,509,469 | G→A | intergenic (‑45/‑159) | glk ← / → yfeO | glucokinase/putative ion channel protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,509,469 | 0 | G | A | 98.4% | 185.2 / NA | 64 | intergenic (‑45/‑159) | glk/yfeO | glucokinase/putative ion channel protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (36/27); minor base C (0/1); total (36/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTTAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAC > NC_000913/2509330‑2509608 | gccTTAGCCCGCGCGTTTGCACCACTGGCAATACCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATaa < 1:573421/141‑1 (MQ=255)gTCTTAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATaa > 2:611004/1‑143 (MQ=255) tCTTAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATaaa > 2:481833/1‑143 (MQ=255) gCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTTCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCt < 1:581587/143‑1 (MQ=255) ccTGCGAGAATTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGAATACTTGGTCATTCTTCAACTGCTCCGCTACAGTCAAAATAATTATTTCTCAAACCATAAATGccgg > 1:627350/1‑141 (MQ=255) cTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCt > 2:433741/1‑141 (MQ=255) cTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCt < 1:433741/141‑1 (MQ=255) tGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTggg < 2:943122/143‑1 (MQ=255) cGAGATTGCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCACTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTggggg < 2:932607/143‑1 (MQ=255) gagaTTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACATGGGGGc > 2:427469/1‑143 (MQ=255) tttCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATaa > 1:837915/1‑143 (MQ=255) ttCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATaac < 2:713945/143‑1 (MQ=255) tCACCACTGGCAATATCACACCGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATaaca < 1:539017/143‑1 (MQ=255) tatCACACAGAGCAAGACGTGCGTTGAAGCCCCCCAAATCACTGACTAGTGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGaaa > 2:835752/1‑143 (MQ=255) cacaGAGCAAGGCGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAaggcag < 2:221786/143‑1 (MQ=255) cacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAaggcaa > 1:1040569/1‑142 (MQ=255) cacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATCCTTCAACTGCTCAGCTAAAGTCAACATAATTCTTTCTCCCACGATAAATACCTGGAAGCATAACAACAACGcaagacagcccg > 1:657547/1‑132 (MQ=255) cacaGAGCAAGACGTGCGTTGGTGCCGCACACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCTGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACATGGGGGCATAACAACAACGCCGGaacgacac > 1:450877/1‑137 (MQ=255) agagCAAGACGTGCGTTGGTGCCGCCCACATCACCGACGAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATACTTCTTTCCCACACAATAAACACCTCGGCGCATACCAACACCCCCGGAAAGCCAGCCt > 1:252223/1‑143 (MQ=255) agCAAGACGTGCCTTGGTGCCCTCCACATCACCGACTAATGCATACTGTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTcc < 1:899522/143‑1 (MQ=255) aGACGTGCGTTGCTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGt < 1:712418/143‑1 (MQ=255) taacaccgaggccgccCACATCACCGACTAATGCATACTATGTCATTCTTCAACTGCTCCGCTAACGTCAACATAATTCTTTCTCACAATATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGACTCCCTGTAAat > 1:588180/11‑143 (MQ=255) ttGGTGCCGCCCGCATCAGCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGa < 1:1044123/143‑1 (MQ=255) gTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGTGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCt < 1:125795/143‑1 (MQ=255) ccACATCACCGACTAATGCATAGTTTGTCTGTTTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATAACGATCTGGGTcac > 1:647993/1‑143 (MQ=255) cACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAAtt > 1:436330/1‑143 (MQ=255) ggcTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATGCGTTCGCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACtt < 1:84624/141‑1 (MQ=255) gACTAATGCATACTTTGTCCTTCTAGGACGACGCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACtt < 2:946188/143‑1 (MQ=255) gACTAATGCATACTTTGTCATTCTTCAACTGCTCCTCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCTTCTATTCATTCGTGATCGATCTGGGTCACACAATTACtt < 1:587064/143‑1 (MQ=255) gACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACtt < 1:848091/143‑1 (MQ=255) aTGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATAGCCGGTGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATGTGGGTCACGGCATTACGATATCg > 1:231144/1‑143 (MQ=255) aTGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCg > 2:739520/1‑143 (MQ=255) gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGtt < 2:736311/143‑1 (MQ=255) aTACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTGTCACACTATAAATACCTGCGGGCTTAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTc > 2:132450/1‑143 (MQ=255) aCTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAACGGCAGGCTCCCGGTAAATGTCGATCTGGGTCACGCAATTACTTTATCGGTTCAg > 2:157280/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGACAGGCAGGCTCCCTGTACATATCGATCCGGGTCACACAATTACGTTCTCGTTTCAGCACCAAtt > 1:833640/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAACGGCAGGCTCCCTGCCCCTCTCGCTCTGGCTCCCACACTTACTTTTTCGTTCCATCCCCAAtt > 2:405760/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAtt > 2:175461/1‑143 (MQ=255) aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATATGGGTCACACAATTACTGCACGGTTTCAGCATCAAtt > 1:610454/1‑143 (MQ=255) ttcttcAACCGCTCCGCCAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTg < 2:70332/143‑1 (MQ=255) ttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAg > 2:795151/1‑143 (MQ=255) tcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGc > 2:220936/1‑143 (MQ=255) tcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGc > 2:455726/1‑143 (MQ=255) tGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGCACATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc > 1:157166/1‑143 (MQ=255) tCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATCGCAGCGATGCCtt > 2:458943/1‑143 (MQ=255) tCCGCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACAACGCAGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTAAAGTTTCAGCACAAATTGCAGCGCTGCCtt > 2:228705/1‑143 (MQ=255) tcTACAGACAAGATAATTCCTTCTCACACTCTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCttttt < 1:786214/142‑1 (MQ=255) gCTAAAGTCAAAATAATTCTTTCTCACACTATAAATACCTGGGGGCATAACAACCAGGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACTATTACTTTATCGGTTCAGCACCAATTGCAGCGATGCCttttt < 1:122285/143‑1 (MQ=255) aaaaTAACTCTTTCTCACACTATAAATACCTGGGGGCATAACAACACCGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTgg < 2:853230/143‑1 (MQ=255) tctttcgCACACTATAGATACCTGGGATCCTTACAACAACGCTGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTa < 2:441161/143‑1 (MQ=255) tctctctcACACAATCAATACCCGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATTGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTa < 2:627350/143‑1 (MQ=255) ctttctCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTGATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa < 1:13409/143‑1 (MQ=255) ctgtctcACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa < 1:611004/140‑1 (MQ=255) tttctCACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAg > 2:790706/1‑143 (MQ=255) tttccgacacTATAAATCCCTGGCGGCATAACAACAACGCCGGAAAGGCATGCTCCCTGTAAATATCGAACTGGGTCACACAATCACTTTATCGTTTAAGCACCAATTGCAGCGATGCCTTCTTGCACGCTGGGCAAACTaac > 1:185859/7‑142 (MQ=255) ctcACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCAGCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTAt > 1:336622/1‑143 (MQ=255) tcACACTATACATACCTCGGCGCATAACAACAACGCCGGAAAGCCAGGCTCCCTGTAAATACCGACCTGGGTCACACAATGACTCTGCCGCTTCAGCAGCACGTGCAGCGAAGCCCGTGTGCACGCCGGGCCAACTAAGTGTc > 1:1062529/1‑143 (MQ=255) tcACACTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCACACTAAGTATc > 2:167060/1‑143 (MQ=255) cacaCTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt < 2:845367/143‑1 (MQ=255) cacaCTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATAGTGCTGGTTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt < 2:776729/143‑1 (MQ=255) cacaCGATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt < 2:501803/143‑1 (MQ=255) acacTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTg > 2:589572/1‑143 (MQ=255) acacTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATAGCGATCTGGGCCACACACTTGCGTCATCGTTGCAGCGCCAATTGCAGCGAGACCTTTTTGCACGCTGGGCAAACTAAGTATcag > 1:251052/1‑141 (MQ=255) acTATAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAc > 2:705068/1‑143 (MQ=255) | GTCTTAGCCTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAC > NC_000913/2509330‑2509608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |