Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1299499 1300697–1299500 2–1199 95 [0] [2] 98 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)90 (1.180) 70/276 0.0 98.9% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 2 (0.030)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300564‑1300840
                                                                                                                                      |                                                                                                                                              
ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                        >  1:1246500‑M2/135‑143 (MQ=255)
  agttataagtatttccttaactgcttctcctcaacatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCaa                                                                                                                                      <  2:611540‑M2/11‑1 (MQ=255)
  agttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCaa                                                                                                                                      <  1:1105869‑M2/11‑1 (MQ=255)
    ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                    <  1:1126515‑M2/13‑1 (MQ=255)
    ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttgctagaggaacgtgctaaATAATAATCAAtt                                                                                                                                    >  2:697487‑M2/131‑143 (MQ=255)
      ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaacaataattgaaatgatattattaattccactgcctttcgtagagcaaagtgctaaCTAATAATCAATTGt                                                                                                                                  >  2:485186‑M2/129‑143 (MQ=255)
       taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                 >  1:669675‑M2/128‑143 (MQ=255)
        aaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTa                                                                                                                                <  2:985035‑M2/17‑1 (MQ=255)
         cGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTCaataaagacatgggctttg                                                                                                                               >  1:1207359/1‑129 (MQ=11)
         aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaattattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                               <  1:1128337‑M2/18‑1 (MQ=255)
         aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                               >  1:266771‑M2/126‑143 (MQ=255)
          ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattcattccactgcctttggtagcggaaagtcctaaATACTAATCAATTGTtaag                                                                                                                              >  2:522530‑M2/125‑142 (MQ=255)
           gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAat                                                                                                                             <  1:321388‑M2/20‑1 (MQ=255)
           aagaaACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAAACGGCTCAAACATGAAGAAATGAAATGACTGAGACAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAaacaagaacatccgctgcgag                                                                                                                             >  2:1091361/1‑130 (MQ=11)
            atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatt                                                                                                                            >  1:1294572‑M2/123‑143 (MQ=255)
                    aactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCa                                                                                                                    >  2:705215‑M2/115‑143 (MQ=255)
                      ctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAtt                                                                                                                  >  1:1251979‑M2/113‑143 (MQ=255)
                          ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgataaATAATAATCAATTGTTAAATTATTGAGCATTTCac                                                                                                              >  2:766118‑M2/109‑143 (MQ=255)
                             tccttaccatcatgttattttctccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactac                                                                                                           <  2:195706‑M2/38‑1 (MQ=255)
                             tcctcaccatcatgttattttcgccacatcataatcctgggcttgctggagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATAGTGCAtttcaacac                                                                                                           >  2:249779‑M2/106‑139 (MQ=255)
                             tcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtactaaATAATAATCAATAGTTAAATTATTGTGCATTTaactaa                                                                                                           >  2:950340‑M2/106‑142 (MQ=255)
                                tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                        >  1:1222940‑M2/103‑143 (MQ=255)
                                 caccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaacgtgctaaATAATAATCAATTGTTAAATTATTGTGCATTGCACTACTGGa                                                                                                       >  1:181402‑M2/102‑143 (MQ=255)
                                  gccatcatgttcttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATAAATTGTTTTATTATTGTGCATTTCACTACTGGaa                                                                                                      <  2:168578‑M2/43‑1 (MQ=255)
                                  accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATCATAATCAATTGTGAAATTATTGTGCATTTCACTACTGGaa                                                                                                      <  1:936755‑M2/43‑1 (MQ=255)
                                  accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                      >  2:1054985‑M2/101‑143 (MQ=255)
                                  accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                      <  1:451774‑M2/43‑1 (MQ=255)
                                  accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                      >  1:725975‑M2/101‑143 (MQ=255)
                                  accataatgttattgtcggcaaaacatattgctgtgcttgttgaagaataatggaaatgatattattaattccactggcgttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                      <  2:148993‑M2/43‑1 (MQ=255)
                                  accagcaggtttttttcgggacttcataatcctgggcttgctgaagcataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                      <  2:565371‑M2/43‑1 (MQ=255)
                                     atcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTg                                                                                                   <  1:287909‑M2/46‑1 (MQ=255)
                                         tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                               >  1:268536‑M2/94‑143 (MQ=255)
                                         tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                               <  2:885562‑M2/50‑1 (MQ=255)
                                         tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcatttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCATTTCTGGAACTGTAAt                                                                                               >  2:876879‑M2/94‑143 (MQ=255)
                                         tgttattttagccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                               <  2:691480‑M2/50‑1 (MQ=255)
                                          gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgccttgggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                              <  2:822343‑M2/51‑1 (MQ=255)
                                           ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                             >  1:898874‑M2/92‑143 (MQ=255)
                                           ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                             >  1:1187881‑M2/92‑143 (MQ=255)
                                               tttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                         <  1:198935‑M2/56‑1 (MQ=255)
                                                ttccccacatcataatcctgggcgtgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                        <  2:689140‑M2/57‑1 (MQ=255)
                                                  cgccacatcataatcctgcgcttgctgaagaataattgaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGa                                                                                      <  1:590229‑M2/59‑1 (MQ=255)
                                                   gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                     <  1:783511‑M2/60‑1 (MQ=255)
                                                    ccgcgtcagaaggctgggctggccgaagcaaacttgcaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATGGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                    <  2:1020876‑M2/61‑1 (MQ=255)
                                                    acacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                    <  1:908506‑M2/61‑1 (MQ=255)
                                                      acagcataagcctgggcttgcggaagaataattgaaatgatattattaattccagtgcgtttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                  <  2:474732‑M2/63‑1 (MQ=255)
                                                         tcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                               <  1:1257100‑M2/66‑1 (MQ=255)
                                                           ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgtctttggtagaggatagtgctaaATAATAATCAATTGTTAGATTGTTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                             <  2:902675‑M2/68‑1 (MQ=255)
                                                           ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAACTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                             >  1:186867‑M2/76‑143 (MQ=255)
                                                                   gggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCaa                                                                     >  1:517061‑M2/68‑143 (MQ=255)
                                                                           tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTTTAtt                                                             >  1:824870‑M2/60‑143 (MQ=255)
                                                                             aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttg                                                           >  1:615567‑M2/58‑143 (MQ=255)
                                                                               gaataattgaaatgatattattaattccacttcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                         <  1:208392‑M2/88‑1 (MQ=255)
                                                                               gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCTTCTTGTAATGGGATCTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                         >  1:626190‑M2/56‑143 (MQ=255)
                                                                               gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                         <  1:702068‑M2/88‑1 (MQ=255)
                                                                                aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaGTAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                        <  1:1111755‑M2/89‑1 (MQ=255)
                                                                                   aatttaaatgatattattaagtccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                     <  1:370520‑M2/92‑1 (MQ=255)
                                                                                   aattgaaatgatattattaattccactgcctttggtagaggaaagtgctagATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAGATGCTTAGCCAATCTCTAtttgattgag                                                     >  2:399530‑M2/52‑142 (MQ=255)
                                                                                   aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                     >  2:779921‑M2/52‑143 (MQ=255)
                                                                                   aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                     <  1:241066‑M2/92‑1 (MQ=255)
                                                                                   aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                     <  2:683813‑M2/92‑1 (MQ=255)
                                                                                     ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                   >  2:216220‑M2/50‑143 (MQ=255)
                                                                                     atggaatgttattattaattccactgcctttggtagaaggaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACCGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                   <  2:54116‑M2/94‑1 (MQ=255)
                                                                                         aatgatattattaattccactgcttttggtagaggaaagtgctaaATAATAATCAATTTTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                               <  2:301035‑M2/98‑1 (MQ=255)
                                                                                              tattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGACACTCTATATATTATTAaattgaaattttt                                          >  1:1344295‑M2/41‑138 (MQ=255)
                                                                                                ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAATGAACTGAAAAAtgttt                                        >  2:1364946‑M2/39‑143 (MQ=255)
                                                                                                  attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATCGCCACGCTAGGCGAACGGCTATGGGATTGACCTGAAAGAtgtttgt                                      >  2:1321057‑M2/37‑143 (MQ=255)
                                                                                                  attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                      >  1:1336594‑M2/37‑143 (MQ=255)
                                                                                                  attaattccactgcctttggtagaggaaagtgcgaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                      <  1:558192‑M2/107‑1 (MQ=255)
                                                                                                   ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                     >  1:750904‑M2/36‑143 (MQ=255)
                                                                                                   ttaagtgcagtgtctttggtagaggaacgtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGTATTGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                     <  1:446397‑M2/108‑1 (MQ=255)
                                                                                                     aattccactgcctttggaagaggaaagggctaaATAATAATCAATTGTTAAATTATCGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                   <  1:422990‑M2/110‑1 (MQ=255)
                                                                                                      attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                  <  1:784037‑M2/111‑1 (MQ=255)
                                                                                                      attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                  >  2:727954‑M2/33‑143 (MQ=255)
                                                                                                         ccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCa                               >  1:204058‑M2/30‑143 (MQ=255)
                                                                                                          cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                              >  1:172133‑M2/29‑143 (MQ=255)
                                                                                                           actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGCTTGTTAAGGCATg                             >  2:510196‑M2/28‑143 (MQ=255)
                                                                                                              gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                          >  1:202578‑M2/25‑143 (MQ=255)
                                                                                                              gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                          <  1:1264217‑M2/119‑1 (MQ=255)
                                                                                                                    ggtagaggaaagtgtgagATAATAATCAATTGTTAAAGTATTGTACATTTCACTAGTGGAACTGTAATCAGAAAAGATAGATATGCTTAGCCAAGCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGc                    <  2:510555‑M2/125‑1 (MQ=255)
                                                                                                                     gtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGGACTGTACTCAGAAAGGATAGACATGCTTAGCCACTCTCTATGTGATTGGATTGGAAGATGTTTGTTCAGGCGTGGGTGGAGGCt                   >  1:363932‑M2/18‑143 (MQ=255)
                                                                                                                     gtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCt                   >  1:1349205‑M2/18‑143 (MQ=255)
                                                                                                                      tagaggcaaatgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGACAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCCagcga                  >  2:194435‑M2/17‑141 (MQ=255)
                                                                                                                       agaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                 <  2:114572‑M2/128‑1 (MQ=255)
                                                                                                                        gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata                <  2:903805‑M2/129‑1 (MQ=255)
                                                                                                                         aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGCAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAACGATGTTTGTTAAGGCATGGATGCAAGCTACAg               >  2:1045998‑M2/14‑143 (MQ=255)
                                                                                                                         aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAGGTTTGTTAAGGCATGGATGCAAGCTATAg               >  2:362854‑M2/14‑143 (MQ=255)
                                                                                                                             aagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc           <  1:363163‑M2/134‑1 (MQ=255)
                                                                                                                             aagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc           <  1:705215‑M2/134‑1 (MQ=255)
                                                                                                                             aagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc           <  1:714527‑M2/134‑1 (MQ=255)
                                                                                                                              agtgcttaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt          <  2:1354924‑M2/135‑1 (MQ=255)
                                                                                                                               gtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTg         <  1:981771‑M2/136‑1 (MQ=255)
                                                                                                                                tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa        <  2:266771‑M2/137‑1 (MQ=255)
                                                                                                                                   taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAc     >  1:217017‑M2/4‑143 (MQ=255)
                                                                                                                                   taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGAAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGCTGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAc     >  2:73927‑M2/4‑143 (MQ=255)
                                                                                                                                    aaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg    >  2:518219‑M2/3‑143 (MQ=255)
                                                                                                                                    aaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAAGGATGCAAGCTATAGATTCTGATACg    <  2:486206‑M2/141‑1 (MQ=255)
                                                                                                                                     aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCCATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGAAATAGATTCTGATCcga   >  2:1084559‑M2/2‑142 (MQ=255)
                                                                                                                                      ataataatCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt  <  1:1299843/143‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                              
CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300564‑1300840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑