Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA98.9% 268.3 / NA 91P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (41/48);  total (42/49)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184403‑4184680
                                                                                                                                           |                                                                                                                                          
cgggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCt                                                                                                                                         <  2:865084/141‑1 (MQ=255)
 tGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                        >  1:284239/1‑143 (MQ=255)
 tGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATAAGCCTTCCGATGAAAGCGGTACGCCGGCAGACATCGTACTGAACCAGCTg                                                                                                                                        >  2:1055373/1‑143 (MQ=255)
  ggCGGTTAAACGCTGTATCCAGCGTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                       <  2:190860/143‑1 (MQ=255)
       ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                  >  2:1096945/1‑143 (MQ=255)
       ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACACCGTACTGAGCCAGCTGGGCgca                                                                                                                                  >  2:662217/1‑141 (MQ=255)
         gaagggcggaTCCAGCCTGGCGACGAGGTGGCAGGGCGTCACGGGAGCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGTGTatc                                                                                                                                <  2:1271823/134‑3 (MQ=255)
         aaaCGCCGTATCCAGCCTGGTGACAAGATGGGGGGGCGCCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAcc                                                                                                                                <  1:1057983/143‑1 (MQ=255)
         aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAACCGGTACGCCGGTAGACATCGCACTGGACCAGCTGGGCGCAcc                                                                                                                                >  1:1253093/1‑143 (MQ=255)
         aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTccc                                                                                                                                >  1:663748/1‑143 (MQ=255)
          aaCGCCGTATCCAGCCTGGTGACACGATGCACGGGAGAGTATGTAGTATGGGGTTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGATGGGCGTACCg                                                                                                                               >  1:260492/1‑143 (MQ=255)
          aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCg                                                                                                                               >  1:1333060/1‑143 (MQ=255)
             gCCGTATCCCTCCGGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTCCTAAGATCCACCCGATCCAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtct                                                                                                                            <  1:345680/143‑1 (MQ=255)
             gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtct                                                                                                                            <  2:64229/143‑1 (MQ=255)
             gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGAGTGTAATTTATAAGATCAACCCGATCGAAGATATGCCTTACGAGGAAACCGGTACGCCGGTAGACAGCGTACTCAACCAGCTGGGCgcaacgacg                                                                                                                            >  2:679748/1‑135 (MQ=255)
              tcgtcgccAGCCTGGTGAGAAGGTGGCAGGTCGTCACGGTAAGGAGGGTGTGATTTCTAAGAGCAACCCGATGGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACATCGTACTGAAACAGCTGGGCGTACCGtctc                                                                                                                           <  2:211428/137‑1 (MQ=255)
               cGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                          <  1:486791/143‑1 (MQ=255)
                 tATCCCGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                        <  2:1135116/143‑1 (MQ=255)
                  aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                       <  1:412188/143‑1 (MQ=255)
                     cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAACAGATGGGGGTAGGGTCTAGTATGaa                                                                                                                    >  2:582179/1‑143 (MQ=255)
                         ctgggtaCAAGCTCGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTGAGATCAGCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATc                                                                                                                <  2:989707/137‑1 (MQ=255)
                          tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               <  1:197247/143‑1 (MQ=255)
                          tGGTGACAAGATGGCAGGTCGCCACGGTAACAAGCGTGTAATTTCCAACATCCACCCGATCGCAGATACGCCTTACGATGAACACCGTCCGCCGCCACACCTCGTACTCACCCCGCCCCGCCCACCGTCTCgccgcaccctcg                                                                                                               >  2:331796/1‑132 (MQ=255)
                          tGGTGACAAGATGGCAGGGCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATAGGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               <  1:583248/143‑1 (MQ=255)
                           ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGGTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCgg                                                                                                              >  1:89441/1‑143 (MQ=255)
                            gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGGAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACCTCGGt                                                                                                             >  1:158536/1‑143 (MQ=255)
                             tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                            <  1:1116848/143‑1 (MQ=255)
                             tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTACGGAATGAACACCGGTc                                                                                                            >  2:1286902/1‑143 (MQ=255)
                             ggtgcaGATGGCAGGTGGCCACGGTAAGACGGGTGTACTTTCTGAGAGCACCCCGATCGAAGATATGCCTTACGATGAACGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                            <  2:405873/138‑1 (MQ=255)
                                 aaGATGGCATGTCGTCACGGTAACAAGGGTGTGATGTCTAAGGTCAACCCGATCGAAGATCAGCCGTACGCTGAAGACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                        <  2:1254966/143‑1 (MQ=255)
                                 aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGAt                                                                                                        >  2:938361/1‑143 (MQ=255)
                                 aaGATGGCAGGTCGTCACGGTAACAAGGGGGTAATTTCTAAGATCAACCCGAGCGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACCTCGTACTGAACCAGCTGGGCGGACGGTCCCGGCGGACCATCGGTCAGAt                                                                                                        >  2:215062/1‑143 (MQ=255)
                                   gATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATcc                                                                                                      <  2:1050897/143‑1 (MQ=255)
                                    aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                     >  1:820666/1‑143 (MQ=255)
                                       gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                  >  2:357037/1‑143 (MQ=255)
                                       gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                  <  1:1096945/143‑1 (MQ=255)
                                        cAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaa                                                                                                 >  2:270998/1‑143 (MQ=255)
                                        cAGGTCGTCACGGTAACAAGGCTGTAATTTCTAAGATCAACCCGAGCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCCGAAGAACATCGGGCacgcccccgaa                                                                                                 >  2:61223/1‑133 (MQ=255)
                                         aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGCTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGACCCTCGgaa                                                                                                >  2:733114/1‑143 (MQ=255)
                                           gtcgccACTGTAACAAGGGTGTAATCTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAcc                                                                                              <  1:1191335/143‑1 (MQ=255)
                                                  cGGTAACAAGGGTGTACTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  1:338782/143‑1 (MQ=255)
                                                  cGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       >  1:475902/1‑143 (MQ=255)
                                                   ggTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCgggg                                                                                      >  1:593995/1‑143 (MQ=255)
                                                    gTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGt                                                                                     >  1:1275929/1‑143 (MQ=255)
                                                      aattagcgtgtAATGTCTAGGGTCGATCCGATCGAGGATATGCCTTACGATGAATTCGGTCCGCCGGTTGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                                   <  2:1325485/136‑1 (MQ=255)
                                                           gggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTg                                                                              >  1:24000/1‑143 (MQ=255)
                                                              tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCACATCCTCGAAACCCACCTGGGTGTGGCGGCGa                                                                           >  2:873096/1‑143 (MQ=255)
                                                              tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCCTCGACACCCACCCGGGGATGGCTGCGa                                                                           >  2:327407/1‑143 (MQ=255)
                                                               gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGag                                                                          >  1:792904/1‑142 (MQ=255)
                                                                tAATTTCTCAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  2:708355/143‑1 (MQ=255)
                                                                tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  1:1040316/143‑1 (MQ=255)
                                                                caaTTTCTAAGATCCACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  1:964160/142‑1 (MQ=255)
                                                                      cgaaGATCAGCCCGATCGAAGATATGCCTTACGAGGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCGGGGGGTACCGTCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc                                                                   <  2:480379/141‑1 (MQ=255)
                                                                           aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                              >  2:239886/1‑143 (MQ=255)
                                                                            tCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                             <  2:820666/143‑1 (MQ=255)
                                                                             cAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGCTCCTCGACACCCACCTGGGTAGGGCTGCGAAAGGTATCGGCGACa                                                            >  1:882310/1‑143 (MQ=255)
                                                                              aagccGATCGGACATTTGCCTGAGGATGGAATAGGTACGCAGGTAGACATCGTACTGAACCGGCTGGGCGTACCGTGTAGTATGAACATCGGTTGGTTCTTCGAAACCCGCCTGATTATGGCTGCGAAAGGTATCGGCGACaa                                                           <  2:164034/140‑1 (MQ=255)
                                                                              aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCCTGGCACTCAAATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                           >  1:310127/1‑143 (MQ=255)
                                                                              aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                           >  1:28257/1‑143 (MQ=255)
                                                                                cccGATCGAAGATATGCCTTACGAGGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                         <  2:294526/143‑1 (MQ=255)
                                                                                 ccGATCGAAGATATGCCTTACGATGCAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTAAGATCCTCGAAACCAACATGGATATGGCTGCGAAAGGTATCGGCGACAAGAt                                                        >  2:1118378/1‑143 (MQ=255)
                                                                                 ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                        >  1:246762/1‑143 (MQ=255)
                                                                                  cGATCGAGGATATGCCGTGCTGTGACAACGGTACGCCGGTAGACTGCGTACTGAACCAGCTCGGCGTACCGTCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                       <  2:618318/143‑1 (MQ=255)
                                                                                    aTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                     <  1:784300/143‑1 (MQ=255)
                                                                                     tcgccgacctctcTTTCGGTGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    <  2:209216/131‑1 (MQ=255)
                                                                                     tCGAGGACATGCCTTCCGGTGGGGACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAGCATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    <  2:935311/143‑1 (MQ=255)
                                                                                     tCGAAGATCTGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAGGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    <  1:776988/143‑1 (MQ=255)
                                                                                       gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGGTCAACGc                                                  >  1:828002/1‑143 (MQ=255)
                                                                                        atggtaggCCTTACGATGAACACAGTACGCCGGTACACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTCGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                                                 <  2:523433/136‑1 (MQ=255)
                                                                                           gtatGCCTTACGATGATAGCGGTACGCCGGTAGTCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg                                              <  2:434191/142‑1 (MQ=255)
                                                                                              tGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACGAGATAAACGCCATGccg                                           >  2:588222/1‑141 (MQ=255)
                                                                                                    aCGATGAAGACGGTACGCCGGTAGACAGCGTACTGCACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                     <  2:373881/143‑1 (MQ=255)
                                                                                                    aCGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGATACCTTTTTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                     <  1:631091/143‑1 (MQ=255)
                                                                                                     cGAGGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACACCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGGTCAACGCCATGCTGAAAcagc                                    <  1:1163038/143‑1 (MQ=255)
                                                                                                      gATGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCTGGGCGTGCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                   <  1:777678/143‑1 (MQ=255)
                                                                                                      gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                   <  2:1275929/143‑1 (MQ=255)
                                                                                                          acaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                               <  2:691176/141‑1 (MQ=255)
                                                                                                           aaaCGGTACGCCGGTAGACATCGTACTGACCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                              <  1:380044/143‑1 (MQ=255)
                                                                                                            aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                             >  1:1121625/1‑143 (MQ=255)
                                                                                                              cGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACTTTCACTTATGATACCCGGTGAAGTCCTCGAAACCCACCTGGGTATGTCCGCTAAGGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                           <  1:505443/143‑1 (MQ=255)
                                                                                                              cGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                           <  1:13553/143‑1 (MQ=255)
                                                                                                                ctACGCCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                         <  1:497210/142‑1 (MQ=255)
                                                                                                                      cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTATGGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATAAACGCGATGCTGAAACAGCAGCAAGAAGTCGCGaaa                   >  1:196849/1‑143 (MQ=255)
                                                                                                                          aGACCTCGGACTGAACCAGCTGGGCGGCCCGTCTCGTATGAACATCGGTCAGAACCACCAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc               <  1:992189/143‑1 (MQ=255)
                                                                                                                          aGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGAGTATGGCTGCGAAAGGCATCGGCGACAAGATCAAGTCCATGCTGGAACAGCAGCAAGAAGGCGCGAGACTgc               >  2:732465/1‑143 (MQ=255)
                                                                                                                           gACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg              <  1:270998/143‑1 (MQ=255)
                                                                                                                                   aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCATCGAGACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGGCACGATCAACGCCAGACTGAAACAGCAGCAAGAAGACACGaaagagccgcaatgca      >  2:1023526/1‑130 (MQ=255)
                                                                                                                                     tGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc    <  1:1106090/143‑1 (MQ=255)
                                                                                                                                     tGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACATGGTTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc    <  2:475902/143‑1 (MQ=255)
                                                                                                                                       aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAGTTCATCCa  >  1:36549/1‑143 (MQ=255)
                                                                                                                                       aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCGTCCa  >  1:541910/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184403‑4184680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: