Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 700,948 A→C I216S (ATT→AGT)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913700,9480AC100.0% 149.7 / NA 50I216S (ATT→AGT) nagCN‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/23);  total (27/23)

AAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATAT  >  NC_000913/700809‑701086
                                                                                                                                           |                                                                                                                                          
aaaGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTgg                                                                                                                                         >  1:657253/1‑143 (MQ=255)
       ccGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTc                                                                                                                                  <  1:762865/143‑1 (MQ=255)
             tGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGGGTCTTCGCAATc                                                                                                                            >  1:1334277/1‑143 (MQ=255)
              ggCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGGTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATcc                                                                                                                           >  1:1148211/1‑143 (MQ=255)
              cgCAGCGTTCACCCAGCGGTTCGACCTGACTCTGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATcc                                                                                                                           <  1:332286/142‑1 (MQ=255)
               gCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCCATACt                                                                                                                          >  2:436214/1‑143 (MQ=255)
                 aGCGTTCACCCAGCGGTTCGACCTGAATACGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGa                                                                                                                        <  1:130538/143‑1 (MQ=255)
                  gCGTTCGCCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGAc                                                                                                                       <  2:928272/143‑1 (MQ=255)
                  gCGTTCCCCGGGCGGTCCGACCTGAATATGGCCAATTTCACCGACGTTCCCGTTGCGGCCAATAAAAAGGCGCCCGTTAGAGATAATCCCGGCCCCGCTCCCTCGATGGACACGCACCAGACTGGAGGCTTCGCAATCCTGAc                                                                                                                       <  2:1200116/143‑1 (MQ=255)
                       cgccccGCGGTTCGACCTGAATATTGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGAGGATGTTAGATATAAGCACGTCGTTGTTGTCGCGATGGACACGAACCAGACTGGAGTCTTCGCAATCCTGACTTGCa                                                                                                                  <  2:288670/141‑1 (MQ=255)
                       cACCCAGCGGTTCGACCTGAATATGGCCAGTTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCa                                                                                                                  <  2:23245/143‑1 (MQ=255)
                       cACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCa                                                                                                                  <  1:1256879/143‑1 (MQ=255)
                               ggTTCGTCCTGAATCTGGCCATTGTCGCCGACGTTGCCGTTGCGGCCAATACAAGTGCGCCCGTTAGAGTTAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAagta                                                                                                          <  2:703016/143‑1 (MQ=255)
                               ggTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCATACTGGAGTCTTCGCAATCCTGACTTGCACCGAagta                                                                                                          <  1:885246/143‑1 (MQ=255)
                                     aCCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGATAGAGATAATCCCGGCCCCGGTTCCGCGATGGAAACGCACCAGACTGGAGTCTTCGCAATCCTGGCTTGCACCGAAAGAGTGccc                                                                                                    >  2:235859/1‑141 (MQ=255)
                                                gCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCcgccagcgcca                                                                                         >  1:851913/1‑143 (MQ=255)
                                                gCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCcgccagcgcca                                                                                         >  1:645355/1‑143 (MQ=255)
                                                gCCAATTTCACCGACGTTGCCGTTGCGGCCAAGAAAAATGCGCCCGTTAGATATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGCAGTAGTGCTCcgccagcgcca                                                                                         >  2:715396/1‑143 (MQ=255)
                                                 ccAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCCCCGAAGTAGTGCTCcgccagcgccag                                                                                        >  1:8650/1‑143 (MQ=255)
                                                 ccAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCcgccagcgccag                                                                                        >  1:146130/1‑143 (MQ=255)
                                                     tttCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTa                                                                                    >  1:1232096/1‑143 (MQ=255)
                                                       tCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACg                                                                                  <  1:662148/143‑1 (MQ=255)
                                                       cccccGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATTGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACg                                                                                  <  2:993851/140‑1 (MQ=255)
                                                                 cgtcgttgcGGCCATTAACACTGCGCCCTTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGa                                                                        <  2:1082022/140‑1 (MQ=255)
                                                                              aagaaaaaTGCGCCCGTCAGAGATAATCCCGGCCCCGGTGTCGCGATGGACACGCACCAGACCGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAgg                                                           <  1:363691/140‑1 (MQ=255)
                                                                              aaTAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAAGCCGGGCTTGCACAGAAGTAGCGCTCCGCCAGCGCCAGACTACGGAAATCGGGACGAATGAGACCgg                                                           >  2:408367/1‑143 (MQ=255)
                                                                                   aaaTGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATTTCGTGACCAACGACACAGGTCACt                                                      >  2:355390/1‑143 (MQ=255)
                                                                                         gaccGTTAGAGATAACCCCGGACCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTaaaa                                                <  1:387357/141‑1 (MQ=255)
                                                                                          cccGTTAGAGATAATCCCGGCCCCGGTTCCGCGCTGGACACGCACCAGTCTTTTGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCCCTTTAacac                                               >  1:576541/1‑143 (MQ=255)
                                                                                                agagATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGttctt                                         >  1:456444/1‑143 (MQ=255)
                                                                                                 gagaTAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCGCTTTAAAACGttcttc                                        >  1:437210/1‑143 (MQ=255)
                                                                                                  agaTAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCCCCAGACTACGGATCTCCTGACGGGCGTAACAGGTCCCTCAAAAACGTTCTTcc                                       >  1:248070/1‑143 (MQ=255)
                                                                                                   gatcctCCCGGCCCCGGTTCCGCGACGGACACGCACCAGACTGCGGTCTCCGCAATCCTGACTTGCACCCAAGTAGTGCTCCGCCAGCGCCAGACGACGTATATCGTAACCAACGAAACTGGCCACTTTAACACGTTCTTCCa                                      <  2:580473/138‑1 (MQ=255)
                                                                                                    aTAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCag                                     >  1:1250477/1‑143 (MQ=255)
                                                                                                    aTAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCag                                     >  1:1173700/1‑143 (MQ=255)
                                                                                                          ccGGCCCCGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCACAGAAAAAGGTAACTTCAAAACGTTCTTCCAGAGCTTc                               >  2:848975/1‑143 (MQ=255)
                                                                                                           cGGCCCCGGGTCCGCGATGGACACGCACCAGACTGGAGTCTCCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCt                              <  1:165625/143‑1 (MQ=255)
                                                                                                               cccGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTGGGGCGCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTGCTTCCAGAGCTTCTACCa                          >  1:860113/1‑143 (MQ=255)
                                                                                                                ccGGTTCCGCGATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAg                         >  1:888932/1‑143 (MQ=255)
                                                                                                                    ttCCGCGATGAGCACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGcccc                     <  2:1099464/143‑1 (MQ=255)
                                                                                                                    tgccGCGATGGACGCGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCGCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGcccc                     <  1:318839/141‑1 (MQ=255)
                                                                                                                    ctCCGCGCTGTACACGTACCATACTGGAGTCTTCGCACTCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGCCTACGGATACCTTGACCCACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGcccc                     <  2:1057304/142‑1 (MQ=255)
                                                                                                                        gcgATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCCGAGCTTCTACCAGCCCCCAGt                 >  1:232412/1‑143 (MQ=255)
                                                                                                                         cgATGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGtt                <  2:657253/143‑1 (MQ=255)
                                                                                                                            tGGACACGCACCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTCCGGCTATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCCGCGCTTCTACCACCCCCCAGTtctc             >  1:542937/1‑143 (MQ=255)
                                                                                                                              gccccGCACCAGACTGGAGTCTGCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCGTCTACCAGCCCCCAGTTTTCTa           <  2:586883/139‑1 (MQ=255)
                                                                                                                                    caccacACTGGAGGCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGaa     <  1:426861/143‑1 (MQ=255)
                                                                                                                                     aCCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACGAACCAAAAAAGAGAATGGAGTGCGGTCTTCCAGAGCTTCTACCCGCCCCCAGCTTCGTACCGGAat    >  2:286442/1‑143 (MQ=255)
                                                                                                                                     aCCAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGAGATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTGCCAGAGCTTCGACCAGCGCCCAGTGTTCtgactgcat    >  1:229843/1‑135 (MQ=255)
                                                                                                                                       cAGACTGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTCCCTGAatat  >  1:880724/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATAT  >  NC_000913/700809‑701086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: