Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,977,578 T→G S328A (TCA→GCA)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,977,5780TG95.3% 242.3 / ‑5.6 85S328A (TCA→GCA) galRgalactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (43/38);  minor base A (4/0);  total (47/38)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TGCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTT  >  NC_000913/2977438‑2977717
                                                                                                                                            |                                                                                                                                           
tgCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCa                                                                                                                                           >  2:934117/1‑143 (MQ=255)
 gCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGCCCTGGCTTTGGCTCTACCAGATAATCGCCCACACCCGGAAATCACTAATGACTTTAGTCCGACGCTGGTACGTCGTCCTACAg                                                                                                                                          >  1:855713/1‑143 (MQ=255)
  cGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAAACGCCCGCGCCCGGAAATCACTAACGTCTCTAGTCCGACGCTGGTACGACGTCACACAgt                                                                                                                                         >  2:456507/1‑143 (MQ=255)
     ccGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGTCTTTGGCCCTGGCTGATAATCGCCCTCTCCCGGTAATCACTAATTTCTTTTGTCCGACGCTGGTACCTAGTCATGTAGTGTc                                                                                                                                      >  1:39961/1‑143 (MQ=255)
     ccGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTc                                                                                                                                      >  1:1341539/1‑143 (MQ=255)
       gcgcCGGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCaa                                                                                                                                    <  2:477868/143‑1 (MQ=255)
         gcCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCCCCCTCCCCCGGAAAACACTAACGTCTTTACTCCGACGCTGGTACGTAGTCATACAGTCTCAAcc                                                                                                                                  >  2:148169/1‑142 (MQ=255)
                caccGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGc                                                                                                                           >  2:353915/1‑143 (MQ=255)
                caccGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGAGTCAACTCCGTCga                                                                                                                           >  2:716935/1‑142 (MQ=255)
                    gTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGa                                                                                                                       <  1:543994/143‑1 (MQ=255)
                      gCGTTACCCAATCGTGACGAGGGCGACCCAGGCTGCCGAACTGGCTGTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAgg                                                                                                                     <  1:1191849/143‑1 (MQ=255)
                       cGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTCCGTCGTCATGCAGTGTCACCTCCGTCGCTggcggc                                                                                                                    >  1:983176/1‑143 (MQ=255)
                         ttACCCAATCGTGACGATGGCGACCCACGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCGCCCGGAATTCAGTAATGTGTTTAGTCGGACGCTGGTACGTGGTCAGGCAGTGTCAACTCCGTCGCTGGAGGCaa                                                                                                                  <  1:604982/143‑1 (MQ=255)
                           cgccAATCGTGACGGTGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATGATCGCCCTCTCCCGGAAATCACTAAGGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGt                                                                                                                <  2:439372/141‑1 (MQ=255)
                           aCCGAATCGTGACGATGGCGCCCCCGGCTTCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAAGCACTAATGTCTTTAGTCCGCCGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGt                                                                                                                <  2:439342/143‑1 (MQ=255)
                            cccAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCCACTCCGGCGCTGGAGGCAAGtc                                                                                                               >  2:384012/1‑143 (MQ=255)
                             ccAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACGCCGTCGCTGGAGGCAAGtca                                                                                                              >  1:1078714/1‑143 (MQ=255)
                             ccAATCGTGACGATGGCGACCCAGGCTGCCGAACAGGACTTGGCACTGGCGGATAATGGCCCTCTCCCGGAAATCACTAATGTCGATAGTCCGACGGTGGTACGTCGTCGTGCGGTGGCGAATCCTGCGGTGGGGGCAAGgca                                                                                                              >  2:1113904/1‑143 (MQ=255)
                              cAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCCAGTCcgccgccagacaaaagacat                                                                                                             >  1:1344361/1‑129 (MQ=255)
                               aaTCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGtcatc                                                                                                            <  1:870954/143‑1 (MQ=255)
                               aaTCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGtcatc                                                                                                            >  1:1174090/1‑143 (MQ=255)
                               aaTCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGtcatc                                                                                                            >  1:658360/1‑143 (MQ=255)
                               aaTCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAAACGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTAGTCATGCAGTGACAACTCCGCGGCTGGAAGCAAGtcatc                                                                                                            >  2:1249228/1‑143 (MQ=255)
                                 tCGGGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTcatcat                                                                                                          <  1:1095605/143‑1 (MQ=255)
                                  cGTGGCGGTGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATg                                                                                                         <  2:877330/143‑1 (MQ=255)
                                   gggACCATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGc                                                                                                        <  2:33117/141‑1 (MQ=255)
                                    tGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCCTGCAGAGTCAACTCTGTCGCTGGAGGCGAGTCACCATCCa                                                                                                       >  2:449511/1‑143 (MQ=255)
                                    tGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCa                                                                                                       >  1:1282894/1‑143 (MQ=255)
                                     gACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTGCGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGACTTCATTCaa                                                                                                      >  2:1240933/1‑143 (MQ=255)
                                         aTGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGGCGGTCCTCATCCAAcccg                                                                                                  >  2:1244837/1‑141 (MQ=255)
                                           ggCGACCCAGGCTGCCGAACTGGCGTTGGCGCTGGCGGCTAATCGCCCTCGCCCGGAACTCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCg                                                                                                <  2:1104872/143‑1 (MQ=255)
                                            gCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGa                                                                                               <  2:1181992/143‑1 (MQ=255)
                                            gCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGa                                                                                               <  1:934117/143‑1 (MQ=255)
                                            gCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTCAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTAGAGGCAAGTCATCATGCAACCAGCGa                                                                                               >  1:420856/1‑143 (MQ=255)
                                                cccAGGCTGCCGAGCTGGCTTTGGCGCTGGCGGAGAATCGCTCTTTCCCTGAAAGCACTTATGTCTTGAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTaa                                                                                           <  1:1063836/143‑1 (MQ=255)
                                                   aGGCTGCCTCACTCGCTTTGGCCCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCg                                                                                        <  2:1146860/143‑1 (MQ=255)
                                                   aGGCTGCCGAACGGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACGAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCg                                                                                        <  2:695691/143‑1 (MQ=255)
                                                    ggCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATACAGTGTCAACTCCATCGCTCGAGGCAAGTCATCATGCAACCAGCGACTAACCGc                                                                                       >  2:352090/1‑143 (MQ=255)
                                                       tGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTACCCGCAgc                                                                                    >  2:212823/1‑142 (MQ=255)
                                                          cGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTaa                                                                                 >  1:675636/1‑143 (MQ=255)
                                                              cTGGCTTTGGCGCGGGCGGGTGATCGCCCGCTCCCGGAAATCACTAATGTCGGTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGGCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCa                                                                             <  2:1207843/143‑1 (MQ=255)
                                                                ggCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAAt                                                                           <  1:615114/143‑1 (MQ=255)
                                                                    ttGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCa                                                                       <  2:733294/143‑1 (MQ=255)
                                                                      tgcgcTGGCGGATATTCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGGCGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAATCGCAGTTAAAGCAATTCCAgc                                                                     <  2:120987/142‑1 (MQ=255)
                                                                      ggCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAgc                                                                     <  1:156103/143‑1 (MQ=255)
                                                                        cgcTGGCGGATAATCGCCCTCGCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAgcgc                                                                   <  2:717042/143‑1 (MQ=255)
                                                                               ggATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCGATTCCAGCGCCCGTact                                                            >  1:1252777/1‑141 (MQ=255)
                                                                                    aTCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttcttc                                                       >  2:970871/1‑143 (MQ=255)
                                                                                     tCGCCCTCTCCCGGAAGTCAATAATGGCATAAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTAAAACGATGGAGGCAGGTGATGATGCAACAGGCGACGTACCGCAGGTAAAGCAATTCCAGCGCCAGTAATtcgtcg                                                      >  2:1107744/1‑143 (MQ=255)
                                                                                     tCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGt                                                               <  2:392685/134‑1 (MQ=255)
                                                                                     tCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGt                                                               >  1:392685/1‑134 (MQ=255)
                                                                                     tCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCg                                                      >  2:844772/1‑143 (MQ=255)
                                                                                      cGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATACCAGCGCCAGTCAttcttctt                                                     >  1:113747/1‑141 (MQ=255)
                                                                                        cccTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCCGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTCATGCTTCGATg                                                   >  2:46013/1‑143 (MQ=255)
                                                                                           tctcCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGGCAACTACGTCGCTGGAGGCAAGTCATCACGCAGCCAGAGACTAACCGCAGTAACAGCAATGACAGAGCAAGTCATTATTCgcagtaa                                                >  2:600626/1‑137 (MQ=255)
                                                                                              cccGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTgg                                             <  2:1341539/143‑1 (MQ=255)
                                                                                               ccGGAGATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc                                            <  2:114545/143‑1 (MQ=255)
                                                                                               ccGGAAATCACTAATGTCCTTAGTCCGACGCTGGTAGAACGCCAGGCACTGTCAACTCCGTCGGTGGACGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc                                            <  2:1321473/143‑1 (MQ=255)
                                                                                                cGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCg                                           >  1:917520/1‑143 (MQ=255)
                                                                                                     aTCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCACTTCCAGCGCCAGTAATCCTTCGAGGGTCTGGCGACGGc                                      >  2:1131315/1‑143 (MQ=255)
                                                                                                      tCACGAGTGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCg                                     <  1:905280/143‑1 (MQ=255)
                                                                                                       cACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCAGGCGACGGCGa                                    >  1:125375/1‑143 (MQ=255)
                                                                                                       cACCAATGGCTTTCGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCCTCCTGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGa                                    <  1:130480/143‑1 (MQ=255)
                                                                                                          tAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATc                                 >  1:757702/1‑143 (MQ=255)
                                                                                                          tAATGTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGAGAATc                                 >  1:890188/1‑143 (MQ=255)
                                                                                                              gTCTTTAGTCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAACACTTCGATGGTCTCGCGACGGCGAATCAccc                             >  1:558149/1‑143 (MQ=255)
                                                                                                                  ttAGTCCGACGCTGGTACTTCGTCATGCAGTGTCAACGCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCTACTAACCGCAGTTAAAGCAATTCCAGCGCCATTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCgcgc                         <  2:634102/143‑1 (MQ=255)
                                                                                                                      tCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCTCTGGAGGAAAGTCTTCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGa                     <  1:990819/143‑1 (MQ=255)
                                                                                                                      tCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTCAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGCCGGCGAATGCACCGCGCCTGa                     >  1:472447/1‑143 (MQ=255)
                                                                                                                      tCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGa                     <  1:665162/143‑1 (MQ=255)
                                                                                                                      tCCGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCCGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTACGGCGCCGGCGCGCCAACCGCGCCTGa                     >  2:618872/1‑143 (MQ=255)
                                                                                                                      tCCCACGCTGGTAGGTCGGCATGCAGTGTCATCTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATGCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGa                     <  1:215366/143‑1 (MQ=255)
                                                                                                                      gacgacgtaccagcgtcgtCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGa                     >  2:12080/14‑143 (MQ=255)
                                                                                                                        cGACGCTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAcc                   >  1:226564/1‑143 (MQ=255)
                                                                                                                           tgCTGGTACTTCGTAAGGAGGTGTCTACTCTGTGGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCAtt                <  2:39961/142‑1 (MQ=255)
                                                                                                                             cTGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTAt              <  1:1028121/143‑1 (MQ=255)
                                                                                                                              tGGTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATc             >  1:489319/1‑143 (MQ=255)
                                                                                                                                gTACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAGGTCATCATGCTACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCaa           <  2:314381/143‑1 (MQ=255)
                                                                                                                                 tagtttgtcATGCGGTGTCATTTCCGTCGCTGGGGGCAAGCCTTCCTTTTACCTGCTACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCaaa          <  2:889259/137‑1 (MQ=255)
                                                                                                                                 tACGTCGTCATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCaaa          <  1:82496/143‑1 (MQ=255)
                                                                                                                                 gaCGCCGTCAGGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCaaa          <  1:148169/142‑1 (MQ=255)
                                                                                                                                    gtcgtcATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCCGCCCATTATCAAACAg       >  1:1323085/1‑143 (MQ=255)
                                                                                                                                     tcgtcATGCAGTGTCAACTCCGTCGCTGGAGGCACGTCATCATGCAACCCGCGACTAACCGCAGTTAAAGCACTTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGCCGGCGAATCAACCGCGCCTGACCATTATCAAACaca      >  1:462717/1‑141 (MQ=255)
                                                                                                                                     tcgtcATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGACTCAACCGCGCCTGACCAtgacaacacaga      >  2:1156451/1‑132 (MQ=255)
                                                                                                                                         cATGCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGGCTGGCGACGGCGAAGCAACCGCGCCTGACCATTATCAAACAGaaagt  >  1:199876/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TGCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTT  >  NC_000913/2977438‑2977717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: