Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,708,718	G→A	6.0%	A600A (GCG→GCA)	rsxC →	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,708,718	0	G	A	6.0%	217.4 / 5.8	83	A600A (GCG→GCA)	rsxC	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (38/40);  new base A (3/2);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

GCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGC‑GCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAA  >  NC_000913/1708431‑1709003                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                gCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGGCCCAAAgcg                                                                                                                                                                                                                                                                                                 >  1:728515/1‑288 (MQ=255)   ccAGGATACACAGCAGATTGTGATTAAAGCGGGCGAACGCCCGGATACACGTGGAAGTATTGCAGCACGGGAGGGCCGTAAAGCGCAAGCAAGA‑GCGAAAAAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTAGCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcg                                                                                                                                                                                                                                                                                               <  1:461608/288‑1 (MQ=37)     aGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa                                                                                                                                                                                                                                                                                            >  2:621023/1‑289 (MQ=255)      ggCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCCCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcccgcca                                                                                                                                                                                                                                                                                            >  1:582325/1‑286 (MQ=255)           cacaGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGa                                                                                                                                                                                                                                                                                      >  1:775368/1‑289 (MQ=255)            acaGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCCGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGaa                                                                                                                                                                                                                                                                                     <  2:382046/289‑1 (MQ=255)                ccTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGc                                                                                                                                                                                                                                                                                 >  2:536824/1‑289 (MQ=255)                ccTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCGGCAACCGc                                                                                                                                                                                                                                                                                 >  1:77742/1‑289 (MQ=255)                 cTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCTGTGCCAAAGCGCGCAAGCTGGAACAGCa                                                                                                                                                                                                                                                                                >  2:271244/1‑289 (MQ=255)                 cTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCCATTGCCCGTGCCAAACCGCGCCAGCGGGAACAGCa                                                                                                                                                                                                                                                                                >  1:315607/1‑289 (MQ=255)                  taatgggATTAAAGCGGGCGAACGCCCGGATAACAGTGAAATTAGTGGAGCACGGAAAGCCCGAAAAGCGCAAGCCAGC‑GCGAAACAGGAAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCaa                                                                                                                                                                                                                                                                               <  1:461071/283‑1 (MQ=255)                        gATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCTCAACCTGGAACAGCAAACGGCt                                                                                                                                                                                                                                                                         >  1:7644/1‑289 (MQ=255)                        gATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCt                                                                                                                                                                                                                                                                         >  2:699340/1‑289 (MQ=255)                        gATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCCGGAACAGCAACAGGcc                                                                                                                                                                                                                                                                         >  2:53886/1‑288 (MQ=255)                          ttAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCCACGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGCGACCCGCACAAGGCTaa                                                                                                                                                                                                                                                                       >  1:394191/1‑289 (MQ=37)                                   gCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAcc                                                                                                                                                                                                                                                              >  2:187459/1‑289 (MQ=255)                                   gCGAACACGCGGATAACAGTGCAATAATTGGAGAACGGGAAGACCGTAAAACGCAAGCCAGA‑GCGAAACAGGCAGAACGGCAGCAAACTAAAGACGTGGCAACCGGAGCTTATCCACCGAAAACTGCCGTTGAGG‑CAGCTATCGCCCGCGCCGAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAc                                                                                                                                                                                                                                                               <  1:271244/288‑1 (MQ=21)                                                     gTGCAATTATTGCAGCGCGGGAAGCCCGTAAAGAGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCCAACTAACGACGCAGCAACCGTCGCTGATCCACGTAATACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CAGGAACAGCAACAGGCTCATGCGGCACCAGAACAACTGGTCCATCCGCGCAAAGCAGCCGCCGCAGCAGCGATTGCCCGTTCTCAGGCGCGAACGCCGGCCCAGCACCAGGCTCTACCGGAGTCAGAAGCACAgctgatc                                                                                                                                                                                                                                              >  1:384539/1‑281 (MQ=255)                                                     gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑cc                                                                                                                                                                                                                                           >  2:441128/1‑289 (MQ=255)                                                     gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAACCGGGAACAGCCACGGGCTAATGCGGAACCCGAAAGACAGGTCGAT‑c                                                                                                                                                                                                                                            >  1:597124/1‑288 (MQ=255)                                                     gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                                                                               >  2:409443/1‑254 (MQ=255)                                                     gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                                                                               <  1:409443/254‑1 (MQ=255)                                                           ttattGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCaa                                                                                                                                                                                                                                     >  2:283291/1‑289 (MQ=255)                                                                gcagcaCGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCCAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAgcc                                                                                                                                                                                                                                 >  2:615097/1‑288 (MQ=255)                                                                    caCGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcg                                                                                                                                                                                                                                                                                               <  1:349386/223‑1 (MQ=255)                                                                    caCGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcg                                                                                                                                                                                                                                                                                               >  2:349386/1‑223 (MQ=255)                                                                     aCGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTc                                                                                                                                                                                                                           >  2:826498/1‑289 (MQ=255)                                                                      cGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAgaagaa                                                                                                                                                                                                                                                       <  1:564086/261‑1 (MQ=255)                                                                      cGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAgaagaa                                                                                                                                                                                                                                                       >  2:564086/1‑261 (MQ=255)                                                                        ggAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGaa                                                                                                                                                                                                                        >  2:348884/1‑289 (MQ=255)                                                                          agagccgtgacgcagaaGCCAAA‑GCGAGACAGGCAGAACTGAAGCGAACTAAAGACGCCGCAACCGCTGCTGATCCAGCTAAAACTGCCGTTGAGG‑AAGCCAGCACCCGCGCCCAAGAGCGGAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGc                                                                                                                                                                                                                      <  1:103852/274‑1 (MQ=17)                                                                          acacccGTAAAGGGCAAGACAGG‑ACAAAAACGGAAGACCTGCAGAAAACTAACGCGGCCGAAACCCTGGCTGACCCAGGTAAAACTGCCGTTGAAG‑CAGCTATCGCACGGGCCAAAGCGCGGAAG‑CAGGAACAGCAACAGGATAATGCGGAACCAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                       <  1:536824/285‑1 (MQ=17)                                                                          aagacgatAAAACGCAGACAAGA‑GCGAAAAGTGCAGAACAAAATGAAAGTAACGACGCAGCAACCGTGGCTGAACCACGAAAAACGGCCGTGGAAG‑CAGATATCACCCGAGGAAAAGCGCGCAAG‑CTGGAACAGCAACAGGCAAATGCGGAACCAGAACAACAGGTCGATCCGCGTAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                       <  1:813086/281‑1 (MQ=17)                                                                          aaGCCCGTAAAGCGAAAGCCAGA‑GCGAAACAGGCAGAACGGCAGCAAACTAAGGACGCAGCAACCGTCGCGGATCCACGTAAAACTGCCGTAGAAG‑CAGCTATCGCCCGGGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAAGCAGCACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                       <  1:361384/288‑1 (MQ=18)                                                                          aaGCCCGCAAAGGGCAAG‑CAGAGGCGAAACAGGGAGAACTGCAGCAAACTAACGACGATACAACGGTGACTAACCCACCAAAAACTGCCGTGGGAG‑CAGCCATGACCGGCGACAAAGCGCGAAAG‑CTGGAACAGCAACAGGATAATACGGAACCAGAACCACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                       <  1:283291/288‑1 (MQ=17)                                                                               cGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTa                                                                                                                                                                                                                 <  2:757531/289‑1 (MQ=255)                                                                               cGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTa                                                                                                                                                                                                                 <  2:766223/289‑1 (MQ=255)                                                                                  aaaGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTAtt                                                                                                                                                                                                               >  2:361384/1‑288 (MQ=255)                                                                                    aGCGAAAGCAAGA‑GCAAAACAGACAGACCTGAGGGAAACTAACGCCGCAGAACCCCTGGCGGACCCACGTAAAACTGCCGTTGAGG‑CAGCTATCGCCCGGCCCACAGCGGGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACACCAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGcc                                                                                                                                                                                                            <  1:699340/289‑1 (MQ=17)                                                                                       gcAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGAAGAACAGGTCGATCCCGCCCAAACCCCCCGTCCAACCCCCTTTTGCCCg                                                                                                                                                                                                          >  2:127434/1‑289 (MQ=21)                                                                                                a‑gCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAAAAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGc                                                                                                                                                                                                 >  2:801607/1‑288 (MQ=255)                                                                                                    gcacccgggagaacgcgagaaaaCTAACGCCGCAGCAGCCGTGGGTGATACTGGAAAAACTGCCGTGCAGC‑CGGCACTCACCCGCGCGAAAACGGCGAAA‑CTGGAACGACAACAGGCTAATGAGGAGGCAGAACCACAGGCCGATCCGCGCAACGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                              <  1:521994/268‑1 (MQ=14)                                                                                                    gcaccaggaagagcgtcagcaAACTAACGAGGGAGAAAACGCGGCTGATCGAGAAAAAACTGCCGCAGAAG‑CAGCGAGGGGCGCGGCCGAAGCGACGAAG‑CTGGAAGAGCAACAGGATACTGCGGAACCAGAACAACTGGCCGATCCGCGCAAAGCCGCCGTGGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                              <  1:337858/272‑1 (MQ=14)                                                                                                    gAAACAGGCAGAACTGCAGCAAACTAACGACGGAGCACCCGTGGCTGATCCCCGTAAAACTGCCGGACAGG‑CAGCTATCGCACGGGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAAGACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGcgcg                                                                                                                                                                                              <  1:621023/288‑1 (MQ=14)                                                                                                    gAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                              <  2:352784/288‑1 (MQ=255)                                                                                                    gAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTG‑CCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                              <  2:681613/287‑1 (MQ=255)                                                                                                         aGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                                                                                                                                                        <  2:775368/289‑1 (MQ=255)                                                                                                         aGGAAGAACGGCAGCAAACGAAGTGCAGAGCACCCGGTGCTAACCAAAGTAAAACGGCAGTAGAAGAGAGC‑AGTGCACGAGCAACAGCGCGGAAG‑CTGGAACAACAACAGGCTAATGCGGAACCAGAAGAACAGGACGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                                                                                                                                                        <  1:813787/289‑1 (MQ=11)                                                                                                            cAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGc                                                                                                                                                                                         <  2:869199/285‑1 (MQ=255)                                                                                                             aGAACGGCAGCAAAGTAACGGCGCAGGAACAGTAGATGAACCACGAAAAATTGCCGATGAAG‑CAGCTATCGCACGCGCCGAAGCGCGCAAG‑CTGGAACAACAACAGGCTAATGCCGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                                                                                                                                                    <  1:615097/289‑1 (MQ=17)                                                                                                                   gcagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                              <  2:692141/289‑1 (MQ=255)                                                                                                                   gcagcaAACTAACGACGCAGCAACCCTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                              <  2:7644/289‑1 (MQ=255)                                                                                                                              aggaagcagcaAACGTTCATAATCAACGTAAAACTGCAGTTGAAG‑AAGCTAGCACCCGCGACAAAGCGCGAAAG‑CTGGAACAGCGACAGGCTAATGCGGAAGCAGAACAAGAGGTCGATCCGCGGAAAGCTGCCGGCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc                                                                                                                                                                   <  1:826498/284‑1 (MQ=17)                                                                                                                                  cgCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGa                                                                                                                                                                <  2:582325/288‑1 (MQ=255)                                                                                                                                       aagcggtgagtGATCGACGAATAACTGACGTTGACG‑CAGCTATCGCCGGGGCCAACGCG‑GCGAGTCTGGACCAGAGACAGGCTAATGCGTAAGCGGAACACCTGGCCGATCCGCGTAAAGCTGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAGGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg                                                                                                                                                           <  1:349672/278‑1 (MQ=17)                                                                                                                                        aaCCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa                                                                                                                                                         <  2:412003/289‑1 (MQ=255)                                                                                                                                          ccGTTGCTGATCCACGTAAAACTGACGTTGAAG‑CAGCTATCGACCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaaca                                                                                                                                                       <  2:766247/289‑1 (MQ=255)                                                                                                                                               gCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                                                                                                                                                  >  2:853410/1‑289 (MQ=255)                                                                                                                                               gCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACCTTAACCACAACCGGCTAATCCGGCGCCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACCCAACCTTGAACAGCAACAGACTactccagccaccacataacagg                                                                                                                                                  >  1:618922/1‑268 (MQ=14)                                                                                                                                               gCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAT‑CCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCaa                                                                                                                                                                                                    <  2:438914/239‑1 (MQ=11)                                                                                                                                               gCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAT‑CCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCaa                                                                                                                                                                                                    >  1:438914/1‑239 (MQ=11)                                                                                                                                                aagaacgaggTAAAAGTGCCGTGGATG‑CGGCTATTGACGAGGCCACAGCGCGCAAG‑CCGGCACAGCCACAGGCTAAGGAGGAACCAGAAGAGAAGGTCGGTCCGCGAAAAGCTGCGGTCGAAGCCGCTAGTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                                                                                                                                                  <  1:243464/279‑1 (MQ=14)                                                                                                                                                    tCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                                                                                                                                             <  2:736891/289‑1 (MQ=255)                                                                                                                                                     ccACGTATAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt                                                                                                                                                 >  2:349672/1‑284 (MQ=255)                                                                                                                                                       aCGAAAAACGACCGTCGAAG‑AAGCGAGCGCCCGCTCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACGGGTCGATCCGCGCAAAACCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCAANAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcg                                                                                                                                          <  2:70466/289‑1 (MQ=14)                                                                                                                                                        cGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCACAGCCCCACAGCTGGAACAGCAACCGGCTAATGCCGAGCCAGAACAACAGGTCGATCcg                                                                                                                                          >  2:781633/1‑288 (MQ=255)                                                                                                                                                        cGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCAGGAACAGCAACAAGCTAATGCCGCGCCAGAACAACAGGTCGATcgg                                                                                                                                          >  2:223549/1‑286 (MQ=255)                                                                                                                                                                                      ccGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc                                                                                                             <  2:480518/288‑1 (MQ=255)                                                                                                                                                                                         cgcCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGa                                                                                                         >  2:86790/1‑289 (MQ=255)                                                                                                                                                                                                      aaG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAc                                                                                                                                                                                                                                                               <  1:320575/127‑1 (MQ=40)                                                                                                                                                                                                            ggAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCGAGAAGAACAGGTCGAGCCGCGCAAAGCCGCCGTCGAACCCGCTCTTGCCCGAGCCAAAGCGC‑GCACACgg                                                                                      >  2:176637/1‑289 (MQ=255)                                                                                                                                                                                                              aaCAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGa                                                                                                                                                                                                                                                                 <  1:176516/118‑1 (MQ=39)                                                                                                                                                                                                                 aGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACa                                                                                                                                                                                                                                                     >  2:320575/1‑127 (MQ=255)                                                                                                                                                                                                                       gggCAAATGCGGACCCAGCAACACAGGCCGTACCGCGTAAAGCCGCCGTCGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGC‑GCAAACGGGAACAGCaa                                                                             <  2:94443/286‑1 (MQ=14)                                                                                                                                                                                                                        ggCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAgaagaa                                                                                                                                                                                                                                                       >  2:176516/1‑118 (MQ=255)                                                                                                                                                                                                                                  gAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGC‑GCAAACGGGAACAGCAACCGGCTAATGCgg                                                                >  2:583110/1‑289 (MQ=255)                                                                                                                                                                                                                                   aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCa                                                                                                                                                                                                                                                                                <  2:482743/82‑1 (MQ=38)                                                                                                                                                                                                                                   aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCa                                                                                                                                                                                                                                                                                >  1:482743/1‑82 (MQ=38)                                                                                                                                                                                                                                        gAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCCAAGCCCGCAAACCGGACCAGCAACCGGCTAATGCCGAGCCCGAACAACAGGTCGAACCACCCAAAGCCGCAGTTGCACCCCCTATTGCCCGCACCAAAGCCC‑ACAAACGGGCACCGCAACCGGCCGATGCGGAACCaa                                                          >  1:817989/1‑288 (MQ=14)                                                                                                                                                                                                                                                     aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGC‑GCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGa                                             >  2:782116/1‑289 (MQ=255)                                                                                                                                                                                                                                                      tCCGGGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGATAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGC‑GCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAt                                            <  2:597124/289‑1 (MQ=255)                                                                                                                                                                                                                                                               aactgccgTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGTGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCGGACCCAGAAGAACAGGTCAAACCGCCCAAAGCCGCCGTTGACCCGGCTATTGCCCACGCCAAA‑CGCAGCAAACCGGCCACGCAATCGGCTGTTGCCGAGCCCGAAAACCAggccaaaccaaccaa                                    >  1:277457/5‑275 (MQ=11)                                                                                                                                                                                                                                                                    ccgTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGC‑GCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCACGCAAAGCTg                               >  2:134888/1‑288 (MQ=255)                                                                                                                                                                                                                                                                           agcagcCATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGC‑GCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                        <  2:315607/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                 tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGC‑GCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCt                 >  2:710184/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                         gggCCAAAGCGCGCAAGCGGGAACAGCAACAGGCAAAAGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGC‑GCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcg         <  2:618922/287‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAACGCGC‑GCAAACGGGCACCGCAACCGGCTCATGCGGACCCAGATGAACCGGTTGACCCGCGCAAAGCTGCCGTCGCAGCCGCTATCGCAccc        >  2:865824/1‑286 (MQ=14)                                                                                                                                                                                                                                                                                                aGCGCGCAAGCTGGAACAGCAACAGGCTAATGAGGAACCAGAACGACAGGTCGAT‑CCGCGCAAAACCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGC‑GCAAACTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCaaa  <  2:77742/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                GCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGA‑GCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAG‑CAGCTATCGCCCGCGCCAAAGCGCGCAAG‑CTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAT‑CCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGC‑GCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAA  >  NC_000913/1708431‑1709003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑