Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,660,944 T→A 4.0% K196* (AAA→TAA)  iscS ← cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,660,9440TA 4.0% 377.1 / 4.4 100K196* (AAA→TAA) iscScysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (43/53);  new base A (2/2);  total (45/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 101 total aligned reads displayed.

CACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCAAAACCTTCGCGCTCCAGCTGACGGCAGGTATCCAGTACCGCTTTGTGTTCGGTTT  >  NC_000913/2660656‑2661228
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                             
cACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACttt                                                                                                                                                                                                                                                                                               >  1:558490/1‑289 (MQ=255)
  ccGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCATACGTTCCATCCCGGTCGCCATCGCCTCTTTTGCGATGCGATAGGCTTAGCCCATTCCGATGATCTGGTGAACAGGAAGCGTGCCGGAACGCATACCGCGCACGGGACCGCCGCCGTGCATTTGCGCTTCTATGCGTACGCGTGCTTTACGACGTACCTACCGACAACCCATACCACTCGGCACATAGCTTTTCTGACCTGACAAAGACATCAGGTCAACTTTCa                                                                                                                                                                                                                                                                                             >  1:429919/1‑289 (MQ=255)
   cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCaa                                                                                                                                                                                                                                                                                            <  2:133809/289‑1 (MQ=255)
   cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCaa                                                                                                                                                                                                                                                                                            <  1:317154/289‑1 (MQ=255)
     ttCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTACa                                                                                                                                                                                                                                                                                             >  2:420149/1‑286 (MQ=255)
       cAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTgg                                                                                                                                                                                                                                                                                        <  1:599932/289‑1 (MQ=255)
                      gATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTACAACTGGCTCAGGTCGATAGGc                                                                                                                                                                                                                                                                         <  1:420149/289‑1 (MQ=255)
                                    cGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTACCCACGctc                                                                                                                                                                                                                                                          <  2:8698/289‑1 (MQ=255)
                                     gTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCAGACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGctc                                                                                                                                                                                                                                                          <  2:290639/289‑1 (MQ=255)
                                      ttCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGc                                                                                                                                                                                                                                                            >  1:397399/1‑286 (MQ=255)
                                      ttCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGc                                                                                                                                                                                                                                                            >  2:169011/1‑286 (MQ=255)
                                      ttCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGc                                                                                                                                                                                                                                                            >  2:234732/1‑286 (MQ=255)
                                         cccaGGCGGTTACGCAGGCCGCGCAGACGTTCCATCTCGTTCGCCATCTCTTGTTTTGCGATGCGATAGGCCTCGCCCACTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTGTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTggg                                                                                                                                                                                                                                                      <  2:843254/287‑1 (MQ=255)
                                              aCGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCa                                                                                                                                                                                                                                                 >  2:871419/1‑289 (MQ=255)
                                               cGGTTAGGCAGGCCGCGCAGACGTGCCATCTCGGCCGCCATCTCTTCTTTGGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCAt                                                                                                                                                                                                                                                <  1:600256/289‑1 (MQ=255)
                                                ggTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCAt                                                                                                                                                                                                                                                >  1:644968/1‑288 (MQ=255)
                                                  ttgcgacgacgggagggaccgtCCATCTCTGTAGCGATCTCGTCGTTTGCGAGGCGATAGGCCTCACCCCTTCCGACGCACTGGTGAACAGGCAGAGGGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGGGCATTTGCGCTTCGAGGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATATTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGGTGCATCa                                                                                                                                                                                                                                              <  1:543293/267‑1 (MQ=255)
                                                   tACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCaa                                                                                                                                                                                                                                             <  1:234732/288‑1 (MQ=255)
                                                      gCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTg                                                                                                                                                                                                                                         <  1:308466/289‑1 (MQ=255)
                                                           ccGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTga                                                                                                                                                                                                                                        <  2:53807/285‑1 (MQ=255)
                                                           ccGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTga                                                                                                                                                                                                                                        >  1:53807/1‑285 (MQ=255)
                                                             gcgcAGACGTTCCATCTCGGTCGCCATCTCTTCTCTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTgatagata                                                                                                                                                                                                                                  <  2:259578/289‑1 (MQ=255)
                                                              cgcAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATaa                                                                                                                                                                                                                                 >  2:843166/1‑289 (MQ=255)
                                                               gcAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAAt                                                                                                                                                                                                                                <  2:644968/289‑1 (MQ=255)
                                                                    cGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGcc                                                                                                                                                                                                                             <  2:483141/287‑1 (MQ=255)
                                                                    cGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCAc                                                                                                                                                                                                                           <  2:665117/289‑1 (MQ=255)
                                                                       tCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAg                                                                                                                                                                                                                       <  1:843166/289‑1 (MQ=255)
                                                                           tctcGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAAt                                                                                                                                                                                                                                >  1:600958/1‑277 (MQ=255)
                                                                           tctcGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAAt                                                                                                                                                                                                                                <  2:600958/277‑1 (MQ=255)
                                                                                 tCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGAACGGcccag                                                                                                                                                                                                             >  1:406003/1‑288 (MQ=255)
                                                                                          cttcttTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGAt                                                                                                                                                                                                    <  2:725931/289‑1 (MQ=255)
                                                                                          cttcttTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGAt                                                                                                                                                                                                    <  2:397399/289‑1 (MQ=255)
                                                                                          cttcttTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGAt                                                                                                                                                                                                    <  2:406003/289‑1 (MQ=255)
                                                                                           ttcttTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATa                                                                                                                                                                                                   >  2:37767/1‑289 (MQ=255)
                                                                                            tcttTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAg                                                                                                                                                                                                  <  2:599659/289‑1 (MQ=255)
                                                                                                 tgctatgcgaTAGGCCTCGACCATTCCGACGATCAGGGTAACAGGCAGAGTGCAGGACCGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg                                                                                                                                                                                             <  2:429919/289‑1 (MQ=255)
                                                                                                 tgcgatgcgaTAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg                                                                                                                                                                                             <  2:558490/289‑1 (MQ=255)
                                                                                                 tgcgatgcgaTAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg                                                                                                                                                                                             >  2:327086/1‑289 (MQ=255)
                                                                                                 tgcgatgcgaTAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg                                                                                                                                                                                             <  2:47640/289‑1 (MQ=255)
                                                                                                 tgcgatgcgaTAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCCCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCCCCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAACGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCACCGTGATACATACTGCCACGCGCACGGCACATTTCGCCGACAGCcgcg                                                                                                                                                                                             >  1:269117/1‑289 (MQ=255)
                                                                                                 tgcgatgcgaTAGGCATCGCCCATTCCGCCGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg                                                                                                                                                                                             <  2:665413/289‑1 (MQ=255)
                                                                                                 ggcggtagcatAGGCCTCGCCCATTCCGACGGTCTGGAGAACAGGCAGAGGGCCGGAACGCAGACCGCGGTCGGGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACTACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg                                                                                                                                                                                             <  1:586583/280‑1 (MQ=255)
                                                                                                       gcgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATc                                                                                                                                                                                        <  1:42540/288‑1 (MQ=255)
                                                                                                                cTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGcaccac                                                                                                                                                                               >  2:253600/1‑288 (MQ=255)
                                                                                                                  cGCCCATTCCGACGATCTGGTGAACAGGCAGACTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTCGAGCTTCGATGCGTACGCGCGGTTTCCGACGTACATACAGCGCACAGATAACTTTCGGGCCATACATTTTGTGACCGGAGAAAGACATCCGGTCAACTTTCAACTGGCTCTGGTCGATAGGCACTTTAACCACGCACTGGGTTCCCTCAATGTGCTCGATACTCAACATAACACGGCACATTCCGCCGCTCGCCGCGTAACCCTGcaccctgc                                                                                                                                                                             >  1:846449/1‑284 (MQ=255)
                                                                                                                        ttCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTc                                                                                                                                                                      >  1:171885/1‑289 (MQ=255)
                                                                                                                             acgaTCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCAACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGttct                                                                                                                                                                 >  1:629660/1‑287 (MQ=255)
                                                                                                                                   tGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCCCGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGt                                                                                                                                                           >  2:679217/1‑289 (MQ=255)
                                                                                                                                   tGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACg                                                                                                                                                                              <  1:11570/270‑1 (MQ=255)
                                                                                                                                   tGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACg                                                                                                                                                                              >  2:11570/1‑270 (MQ=255)
                                                                                                                                       gAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCa                                                                                                                                                        <  2:171885/288‑1 (MQ=255)
                                                                                                                                                  gTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGAt                                                                                                                                                                                                    <  2:600040/233‑1 (MQ=255)
                                                                                                                                                  gTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGAt                                                                                                                                                                                                    >  1:600040/1‑233 (MQ=255)
                                                                                                                                                  gTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGaca                                                                                                                                               <  1:871419/286‑1 (MQ=255)
                                                                                                                                                  gTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGaca                                                                                                                                               <  2:818270/286‑1 (MQ=255)
                                                                                                                                                      cGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGAt                                                                                                                                        <  1:37767/289‑1 (MQ=255)
                                                                                                                                                           cGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCa                                                                                                                                                                                                                                              >  1:728295/1‑183 (MQ=255)
                                                                                                                                                           cGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCa                                                                                                                                                                                                                                              <  2:728295/183‑1 (MQ=255)
                                                                                                                                                               tACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTAccc                                                                                                                                                                                                                                                                >  2:84388/1‑161 (MQ=255)
                                                                                                                                                               tACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTAccc                                                                                                                                                                                                                                                                <  1:84388/161‑1 (MQ=255)
                                                                                                                                                                aCCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTACAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTgtcgtc                                                                                                                               >  1:580422/1‑288 (MQ=255)
                                                                                                                                                                   gcgcTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg                                                                                                                                                                                             >  2:658086/1‑223 (MQ=255)
                                                                                                                                                                   gcgcTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg                                                                                                                                                                                             <  1:658086/223‑1 (MQ=255)
                                                                                                                                                                    cgcTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg                                                                                                                                                                                             >  2:582623/1‑222 (MQ=255)
                                                                                                                                                                    cgcTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg                                                                                                                                                                                             <  1:582623/222‑1 (MQ=255)
                                                                                                                                                                      cTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCa                                                                                                                                                        <  1:48951/257‑1 (MQ=255)
                                                                                                                                                                      cTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCa                                                                                                                                                        >  2:48951/1‑257 (MQ=255)
                                                                                                                                                                      cTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATc                                                                                                                        >  2:510433/1‑289 (MQ=255)
                                                                                                                                                                                ccgccgTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCaa                                                                                                              >  2:364744/1‑289 (MQ=255)
                                                                                                                                                                                            tgggcTTCGTGGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGt                                                                                                  <  2:1606/286‑1 (MQ=255)
                                                                                                                                                                                                  tCGATGCGTACGGGGGGTTTACGACGTACATACAGCGCACCGATACCTGTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATaa                                                                                            <  1:169011/289‑1 (MQ=255)
                                                                                                                                                                                                          tACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGtt                                                                                     >  1:796324/1‑288 (MQ=255)
                                                                                                                                                                                                                cgggtgacaacgTACATACAGCGAACCGATACCTTACGGGCCATAGAGTTTGTGGCCGGAGAAAGACATCAGGGCCACTTTCAACGGGCTAAGGTGTATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTg                                                                               <  1:679217/279‑1 (MQ=255)
                                                                                                                                                                                                                    ttACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTg                                                                          <  2:67168/289‑1 (MQ=255)
                                                                                                                                                                                                                             acatacaGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAAt                                                                                                                                                                                                                                >  2:288493/1‑131 (MQ=255)
                                                                                                                                                                                                                             acatacaGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAAt                                                                                                                                                                                                                                <  1:288493/131‑1 (MQ=255)
                                                                                                                                                                                                                             acatacaGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCg                                                                                       <  1:674068/267‑1 (MQ=255)
                                                                                                                                                                                                                             acatacaGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCg                                                                                       >  2:674068/1‑267 (MQ=255)
                                                                                                                                                                                                                             acatacaGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCaggtagg                                                                 >  2:76041/1‑289 (MQ=255)
                                                                                                                                                                                                                             acatacaGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCaggtagg                                                                 >  1:530060/1‑289 (MQ=255)
                                                                                                                                                                                                                              catgcaGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCaggtaggt                                                                <  1:754695/289‑1 (MQ=255)
                                                                                                                                                                                                                                 acaGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACCTTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCCAGTTCTTTCAGGTCGATCATGCCGTTACGCTGCGGTGCCaggtaggtc                                                               >  1:874647/1‑286 (MQ=255)
                                                                                                                                                                                                                                     cgcACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGTATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCa                                                         >  2:511038/1‑289 (MQ=255)
                                                                                                                                                                                                                                     cgcACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCa                                                         >  2:150465/1‑289 (MQ=255)
                                                                                                                                                                                                                                     cgcACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCAGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTGGGTGACTTCa                                                         >  1:804471/1‑289 (MQ=255)
                                                                                                                                                                                                                                      gcACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCgg                                                                            >  1:879798/1‑269 (MQ=255)
                                                                                                                                                                                                                                        aCCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGacac                                                                                                                                              <  1:194850/201‑1 (MQ=255)
                                                                                                                                                                                                                                        aCCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGacac                                                                                                                                              >  2:194850/1‑201 (MQ=255)
                                                                                                                                                                                                                                            aTACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCg                                                                                                                       <  2:49347/220‑1 (MQ=255)
                                                                                                                                                                                                                                            aTACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCg                                                                                                                       >  1:49347/1‑220 (MQ=255)
                                                                                                                                                                                                                                                          aTAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCg                                                                                       <  1:180578/238‑1 (MQ=255)
                                                                                                                                                                                                                                                          aTAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCg                                                                                       >  2:180578/1‑238 (MQ=255)
                                                                                                                                                                                                                                                                tttGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCAAAACCTTCGCGCTCCAGCTGACGGCAg                              <  1:510433/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                tttGTGACCGGAGAAAGACATCAGGTCAAAGTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCAAAACCTTCGCGCTCCAGCTGACGGCAg                              <  1:662841/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                 ttGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCAAAACCTTCGCGCTCCAGCTGACGGCAgg                             >  2:743224/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  tgtgACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGGCGTTACGCTGCGGTTCCAGGTAGGTGACTTCAAAACCTTCCCGCCCCAGcggcagggatgt                            >  1:308772/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                   gtgACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCAAAACCTTCGCGCTCCAGCTGACGGCAGGTa                           <  2:796324/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gACCGGAGAAAGACATCAGGTCAACTTACAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCAAAACCTTCGCGCTCCAGCTGACGGCAGGTATc                         <  2:580422/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    tCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCAAAACCTTCGCGCTCCAGCTGACGGCAGGTATCCAGTACCGCTTtgtg          >  2:63271/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                            aCTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCAAAACCTTCGCGCTCCAGCTGACGGCAGGTATCCAGTACCGCTTTGTGTTCGGttt  <  1:364744/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                             
CACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGATATCCTGCACCACGCCGATTTCGTTATTTACGTGCATGATGGACACGAGGATGGTGTCGTCACGCATCGCTGCTTCAAGTTCTTTCAGGTCGATAATGCCGTTACGCTGCGGTGCCAGGTAGGTGACTTCAAAACCTTCGCGCTCCAGCTGACGGCAGGTATCCAGTACCGCTTTGTGTTCGGTTT  >  NC_000913/2660656‑2661228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: