Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,817,768 G→A 4.0% intergenic (‑267/+14) yqaB ← / ← argQ fructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,817,7680GA 4.0% 348.2 / 3.7 99intergenic (‑267/+14)yqaB/argQfructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (38/57);  new base A (2/2);  total (40/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

CTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGA  >  NC_000913/2817483‑2818051
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                 
cTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGtt                                                                                                                                                                                                                                                                                                <  1:317521/289‑1 (MQ=255)
    aTAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTGCTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACgg                                                                                                                                                                                                                                                                                            <  2:715555/288‑1 (MQ=255)
               aCATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGt                                                                                                                                                                                                                                                                                  <  2:832127/288‑1 (MQ=255)
                     tcttctCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCg                                                                                                                                                                                                                                                                           >  2:36517/1‑289 (MQ=255)
                         ctcGCAAGTCGAAAAATATGACGGTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGACATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGTCGCCACGTTATACTTTCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGTATCTGGTGCATCCGggag                                                                                                                                                                                                                                                                       >  1:156111/1‑289 (MQ=255)
                                              cGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGTGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCATTCGGGCCGTTGCTGGCGCAACGTTATACTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                                                                                   <  2:556385/288‑1 (MQ=255)
                                              cGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGTATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                                                                                   <  2:479151/288‑1 (MQ=255)
                                                    tttAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAGATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                                                                                                                                                                                                            <  2:501564/289‑1 (MQ=255)
                                                    tttAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                                                                                                                                                                                                            <  2:333435/289‑1 (MQ=255)
                                                           tAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                                                                                                                                                                     <  1:486502/289‑1 (MQ=255)
                                                           tAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                                                                                                                                                                     <  1:871805/289‑1 (MQ=255)
                                                            aagtGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAAGTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGGGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                                                                                                                                                                     <  1:246345/285‑1 (MQ=255)
                                                             aTTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGt                                                                                                                                                                                                                                   >  2:710220/1‑289 (MQ=255)
                                                             aTTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGt                                                                                                                                                                                                                                   >  2:157857/1‑289 (MQ=255)
                                                             aTTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGATATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                                                                                                                                                                          >  2:533479/1‑282 (MQ=255)
                                                             aTTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGATATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                                                                                                                                                                          <  1:533479/282‑1 (MQ=255)
                                                                gCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAct                                                                                                                                                                                                                                <  1:780879/289‑1 (MQ=255)
                                                                          gTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                                                                                                                                                                      <  2:111619/289‑1 (MQ=255)
                                                                          gTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                                                                                                                                                                      <  2:298681/289‑1 (MQ=255)
                                                                                        aGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCa                                                                                                                                                                                                        <  1:187791/289‑1 (MQ=255)
                                                                                         ggAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCa                                                                                                                                                                                                        >  1:624317/1‑288 (MQ=255)
                                                                                             aagaaAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAata                                                                                                                                                                                                                                                                                      <  2:178091/206‑1 (MQ=255)
                                                                                             aagaaAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAata                                                                                                                                                                                                                                                                                      >  1:178091/1‑206 (MQ=255)
                                                                                              agaaAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCggg                                                                                                                                                                                                   <  2:441186/288‑1 (MQ=255)
                                                                                                     tCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTa                                                                                                                                                                                           <  1:157857/289‑1 (MQ=255)
                                                                                                         gAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTc                                                                                                                                                                                        >  2:52328/1‑288 (MQ=255)
                                                                                                          aaaTGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCt                                                                                                                                                                                       <  2:154637/288‑1 (MQ=255)
                                                                                                           aagggATGAATGAGTGATTTAGAAAGGTGTTATTTTTGAGGGCAATTTGTGGGAGATGTGGGGGCATCGGGGAGGTTGCCTGACGGTCGTGCGCCCTTCGGGGCGTTGCGGTCTCAACGTTATCCTTCCTGGTGCGTGCAGTTAATACCGCTATGTCTTCGATACCTTCATTGCTGAAGGTTACGTGGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTTAGCTACGGATGCATCGGGAAAACTTATTCTa                                                                                                                                                                                      <  1:657466/285‑1 (MQ=17)
                                                                                                               gATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                                                                                                 <  2:733620/288‑1 (MQ=255)
                                                                                                                aTAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTActgct                                                                                                                                                                                 <  2:716845/288‑1 (MQ=255)
                                                                                                                         ttATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGAt                                                                                                                                                                      >  2:539138/1‑288 (MQ=255)
                                                                                                                         ttATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGAt                                                                                                                                                                      >  2:53226/1‑288 (MQ=255)
                                                                                                                         ttATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTGGCTGGCGCAACGTTATCCTTCCGGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATCTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCCCGGGTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGAATCGGGATAAATTATTCTActggcgatcttccc                                                                                                                                                                        >  2:657466/1‑277 (MQ=21)
                                                                                                                            tttAGAAATTGGATATGTTGGAAGGAATGTTATTGGGGAAGAGGTTGCGTCTGGGAGGATGACTCACTTACGTTTGCCCTTCGAGCCGTTGCTGGCGCTACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCAGTGCTGAAGGTTACGGAGAATATGGTGAATCCTGGAGGATTCGAACCTCCGATCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCg                                                                                                                                                                  <  1:110755/289‑1 (MQ=18)
                                                                                                                            tttAGAAATTAGTTATGTTTGAAGGAAATTTTTTGGAGAAGTTGGTGCATCCGGGAGGATGACTCACTGACGTTAGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCg                                                                                                                                                                  <  1:340774/289‑1 (MQ=255)
                                                                                                                            tttAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTAc                                                                                                                                                                                                                                                                                              >  2:358816/1‑167 (MQ=255)
                                                                                                                            tttAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTAc                                                                                                                                                                                                                                                                                              <  1:358816/167‑1 (MQ=255)
                                                                                                                                 aaaTGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                                                                                                                                                                     <  1:53093/219‑1 (MQ=255)
                                                                                                                                 aaaTGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                                                                                                                                                                     >  2:53093/1‑219 (MQ=255)
                                                                                                                                  aaTGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGctact                                                                                                                                                             <  2:313653/288‑1 (MQ=255)
                                                                                                                                      agaTATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAGGGCCCGGTTTGTAGCCGAGTACTCTATCCTGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGAGGA‑‑TATTGATACCGTTACTAAAg                                                                                                                                                         >  1:544909/1‑288 (MQ=255)
                                                                                                                                      agaTATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                                                                                                                                                                     <  1:873145/230‑1 (MQ=255)
                                                                                                                                      agaTATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                                                                                                                                                                     >  2:873145/1‑230 (MQ=255)
                                                                                                                                          atTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCata                                                                                                                                                    <  1:539138/289‑1 (MQ=255)
                                                                                                                                           tttttGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGGTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCatat                                                                                                                                                   <  1:179163/289‑1 (MQ=255)
                                                                                                                                              ttGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCaa                                                                                                                                                >  1:650738/1‑289 (MQ=255)
                                                                                                                                                gAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAtt                                                                                                                                              <  1:710220/289‑1 (MQ=255)
                                                                                                                                                   ggAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAag                                                                                                                                           >  2:403190/1‑289 (MQ=255)
                                                                                                                                                           tttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTg                                                                                                                                  <  1:36517/289‑1 (MQ=255)
                                                                                                                                                                   agaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCg‑‑‑g                                                                                                                        <  1:53226/288‑1 (MQ=255)
                                                                                                                                                                      tGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑ggag                                                                                                                     <  2:624317/288‑1 (MQ=255)
                                                                                                                                                                              ccGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTActgct                                                                                                                                                                                 <  1:664607/226‑1 (MQ=255)
                                                                                                                                                                              ccGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTActgct                                                                                                                                                                                 >  2:664607/1‑226 (MQ=255)
                                                                                                                                                                                  gaggaTGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCggg                                                                                                                                                                                                   <  1:489094/204‑1 (MQ=255)
                                                                                                                                                                                  gaggaTGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCggg                                                                                                                                                                                                   >  2:489094/1‑204 (MQ=255)
                                                                                                                                                                                  gaggaTGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTCCTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCCAGTAAGAGATGGTGCATCCG‑‑‑GGACGATTAATCGCCt                                                                                                         >  1:127526/1‑289 (MQ=17)
                                                                                                                                                                                   aggaTGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCtt                                                                                                        <  2:304582/288‑1 (MQ=255)
                                                                                                                                                                                    ggaTGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGAGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTg                                                                                                       <  2:156111/288‑1 (MQ=255)
                                                                                                                                                                                          cTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGATGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCg                                                                                                                                                                  <  2:551886/227‑1 (MQ=255)
                                                                                                                                                                                          cTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGATGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCg                                                                                                                                                                  >  1:551886/1‑227 (MQ=255)
                                                                                                                                                                                               tgccGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCtt                                                                                           <  2:544909/286‑1 (MQ=255)
                                                                                                                                                                                                gACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                          >  2:453565/1‑289 (MQ=255)
                                                                                                                                                                                                 aCGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTCTTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAGGATTACTCGCCTTGCGGCTCGCCCt                                                                                            >  2:255514/1‑289 (MQ=255)
                                                                                                                                                                                                     tCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGcc                                                                                     <  2:650738/289‑1 (MQ=255)
                                                                                                                                                                                                      cGCCCTGCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGcc                                                                                     <  2:127526/289‑1 (MQ=17)
                                                                                                                                                                                                                    ttGCTGGCGCAACGGTATCCTTCCTGGTGGCTGCGATTGATACCGCTATCTCTTCGATACCGTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACg                                                                      <  1:803704/289‑1 (MQ=255)
                                                                                                                                                                                                                                cGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                                                                                                                                                                                  <  1:295944/127‑1 (MQ=255)
                                                                                                                                                                                                                                cGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                                                                                                                                                                                  >  2:295944/1‑127 (MQ=255)
                                                                                                                                                                                                                                cGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTActgca                                                                                                                                                                                 >  2:310355/1‑173 (MQ=14)
                                                                                                                                                                                                                                cGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTActgca                                                                                                                                                                                 <  1:310355/174‑2 (MQ=14)
                                                                                                                                                                                                                                          cctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                                                                                                                                                                   >  2:613437/1‑132 (MQ=255)
                                                                                                                                                                                                                                          cctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                                                                                                                                                                   <  1:613437/132‑1 (MQ=255)
                                                                                                                                                                                                                                               tGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                    >  1:497780/1‑280 (MQ=255)
                                                                                                                                                                                                                                               tGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                    <  2:497780/280‑1 (MQ=255)
                                                                                                                                                                                                                                                   tGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaa                                                                                                                                                                                                >  1:111887/1‑142 (MQ=39)
                                                                                                                                                                                                                                                   tGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaa                                                                                                                                                                                                <  2:111887/142‑1 (MQ=39)
                                                                                                                                                                                                                                                   tGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATAc                                                                                                                                                                    >  2:33408/1‑168 (MQ=255)
                                                                                                                                                                                                                                                   tGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATAc                                                                                                                                                                    <  1:33408/168‑1 (MQ=255)
                                                                                                                                                                                                                                                        ttAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                                   <  1:403190/288‑1 (MQ=255)
                                                                                                                                                                                                                                                              ccGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCg                            <  1:453565/289‑1 (MQ=255)
                                                                                                                                                                                                                                                               cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCAACTGCTGA‑‑TATTGATACTGCTACTTATACCCAATCGACTCTGCCGACGACCCCTTG‑‑‑GGTGGATTACTCGGCTTTCGGCGCGCCCTTAGGGCCGTATCTAAAGCAACGTTATCCTCGCTTGTGCCTGCGGTTCATTACACTTCCTCtttgt                           >  1:366153/1‑286 (MQ=14)
                                                                                                                                                                                                                                                                 cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                                                                                                                                                                      >  1:397590/1‑106 (MQ=40)
                                                                                                                                                                                                                                                                 cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTCATTCCGCCACCCCTTCGAt                          >  2:378032/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                         tCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATTCATCGGGACAACTTCTTCTACCGCTTA‑‑TTTTGCTATCCTTACTAAACCCCTATCAATTAAGAAGTGTTCCATCCG‑‑‑GGAGGTTTACCCCCCTTCCGCCTAGGCCTCTGGGCCGCTCCCAAAGCAACGTTCACACCCCTGGTGCCTGCGGGTATTACCGcccacccttcctttcctgcc                   >  1:754870/1‑268 (MQ=9)
                                                                                                                                                                                                                                                                         tCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                    <  1:47760/254‑1 (MQ=255)
                                                                                                                                                                                                                                                                         tCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                    >  2:47760/1‑254 (MQ=255)
                                                                                                                                                                                                                                                                            aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                    >  2:854684/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                            aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                    <  1:854684/251‑1 (MQ=255)
                                                                                                                                                                                                                                                                                cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                                                                                                                                                                                                            <  1:265423/69‑1 (MQ=40)
                                                                                                                                                                                                                                                                                cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                                                                                                                                                                                                            >  2:265423/1‑69 (MQ=40)
                                                                                                                                                                                                                                                                                cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                                                                                                                                                                                                            >  1:149860/1‑101 (MQ=35)
                                                                                                                                                                                                                                                                                cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                                                                                                                                                                                                            <  2:149860/101‑1 (MQ=35)
                                                                                                                                                                                                                                                                                cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                           >  1:547543/1‑272 (MQ=255)
                                                                                                                                                                                                                                                                                cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                           <  2:547543/272‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  tCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAtt       <  2:831693/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  tCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAtt       >  1:831693/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     ttGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc     <  2:115857/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       gCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCgg   <  2:686581/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        cTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga  >  2:56785/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                 
CTGCATAACGCTCGTACATGGTCTTCTCGCAAGTCGAAAAATATGACGCTACTTTAGCGTAATTGCTTGATTTTGTCGCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG‑‑‑GGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGA  >  NC_000913/2817483‑2818051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: