Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
JC | NC_000913 | 4,001,644 | Δ5 bp | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
New junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 4001643 | 1 (0.020) | 68 (1.080) | 53/284 | 0.1 | 98.6% | coding (219/951 nt) | corA | magnesium/nickel/cobalt transporter |
? | NC_000913 | 4001649 = | 1 (0.020) | coding (225/951 nt) | corA | magnesium/nickel/cobalt transporter |
AGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCG > NC_000913/4001502‑4001777 | aGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATc > 2:936168‑M1/1‑142 (MQ=255) gTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATct > 2:585209‑M1/1‑141 (MQ=255) tATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctc < 2:701464‑M1/143‑4 (MQ=255) aTGGATTGATCTTGTCGAAGCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctcc < 1:200611‑M1/143‑5 (MQ=255) ggtttgatCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctcctt < 1:203920‑M1/140‑7 (MQ=255) gATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGACTCGCCACGTGTCTGTGAAGACGACGACGGCCTGCATctccttc > 2:554109‑M1/1‑136 (MQ=255) aTTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCCACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTACTTTGCAGACGACGACGGCCTGCATctccttct > 2:589964‑M1/1‑135 (MQ=255) tGATCGTGTCGATCTGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGATATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttc < 2:843309‑M1/143‑11 (MQ=255) aTCTTGACGAACCGGACGACGACGAGCGCCTGCGCGCACAATCTGAACTTGGCCAGAGGCTGGCAACCCGCCCGGAACTGGAAGACACCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttctt < 1:71508‑M1/143‑13 (MQ=255) cTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttct < 2:655586‑M1/143‑15 (MQ=255) tCGACCCGGACGACGCGTAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttga < 2:1062348‑M1/143‑19 (MQ=255) tCGAACCGGACGACGACGAGCGGCTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttga < 2:350113‑M1/143‑19 (MQ=255) tCGAACCGGACGACGACGAGCGACTGCGAGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttga < 2:161240‑M1/143‑19 (MQ=255) cagaccgGCCCACGACGAGCCCCTGCGCGTACACTCTGAACTTGGCCAGCGCCTGGCAACCCGCCCGGAACTGGAAGCCATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaa < 2:420977‑M1/140‑20 (MQ=255) cGAACCGGACGACGGCGAGCGACTGCGCGTACAATCGGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACAGCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaa < 1:708339‑M1/143‑20 (MQ=255) aCCGGACGACCACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACCTAGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagat < 2:156065‑M1/143‑23 (MQ=255) cGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgc < 1:632329‑M1/143‑25 (MQ=255) ggACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaaaataca > 2:886615‑M1/1‑118 (MQ=255) gacgacgacGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcgg > 2:701507‑M1/1‑117 (MQ=255) cgacgactaGCGACTGCTCGTACAATCTGACCTTGGCCAGAGCCTTGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaa < 2:703406‑M1/143‑29 (MQ=255) cgacgacgaGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaa < 2:593389‑M1/143‑29 (MQ=255) cgacgacgaGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaa < 2:84048‑M1/143‑29 (MQ=255) gccgacgcgcGACTGCGCGTACCATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGCAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaag < 1:1011974‑M1/141‑30 (MQ=255) gacgaGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggacgatc > 2:648453‑M1/1‑111 (MQ=255) gaGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCATGGATttctggtttcgtctttgaagatgcggaaaatcacg > 2:741686‑M1/1‑108 (MQ=255) gtctgCGCGTACACTCTGAACTTGGCTAGACCCTGGCAACCCGCCCGCAACTGGAAGACATCGAAGCATCGCCACGTTTCTTTGAAGACGACGACGGCCTGCATctccctcttcttctttgaagatgcggaagatcacgccgg < 1:290308‑M1/141‑40 (MQ=255) gcgGACAATCGGAACGTCGCCAGGGCCTGCCCTCGCTCTCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactc < 2:848928‑M1/143‑46 (MQ=255) cgcacgacctgaacttgGCCAGAGCCTGGCACCCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttctgcgtggaagatgcggaagatcacgccggtaactcc < 1:898094‑M1/135‑47 (MQ=255) aCAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccact < 2:654764‑M1/143‑50 (MQ=255) tCTGAACTTTGCCACAGCCTGGCAACCCGTCTGGAACTGGAAGACATGGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtgg < 1:1098462‑M1/143‑54 (MQ=255) gAACTCTGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGACGCATCGGCACGTTTCTTTGATGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcat < 2:573048‑M1/143‑57 (MQ=255) cTTGTCCAGACCCTGGCGTCCCGCCTGGAACTGCAAGACTTGGAAGCATCGGCACGTTTCTTTGAAGACGAGGACGGCCTGCATctccttcttcttcctggatgatgcgtaagatcacgccggtaactccactgtggcattta < 1:333554‑M1/143‑60 (MQ=255) ttGGCAATAGCCGGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttac < 1:59145‑M1/143‑61 (MQ=255) gtgGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACCTCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttac < 1:878019‑M1/142‑61 (MQ=255) gggccGGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgt < 1:1044060‑M1/141‑68 (MQ=255) gagCCTGGCAACCCGCCCGGAACTGGAATACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgt < 2:479792‑M1/143‑68 (MQ=255) gagCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgt < 2:487537‑M1/143‑68 (MQ=255) gCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtga < 2:471153‑M1/143‑70 (MQ=255) gCCTGGCAACCCGCCCGGAACGCGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtga < 1:920941‑M1/143‑70 (MQ=255) ccTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtacctccactgtggcatttaccatccgtgat > 1:753460‑M1/1‑73 (MQ=255) ccTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactcccctgtggcatttaccctccgtggt > 2:665531‑M1/1‑73 (MQ=255) tGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagctcacgccggtaactccactgtggcacttaccatccgctatcg > 1:286393‑M1/1‑71 (MQ=255) tGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatgg < 1:584325‑M1/143‑73 (MQ=255) cccgcccgGAGCTGGCAGCCAGCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtct < 1:1002175‑M1/143‑79 (MQ=255) cccgcccgGAACTGGAAGACATCGAAGCATCGTCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttcttttaagatgcggaagatcacgccagtaactccactgtggcatttaccatccgtgatggtcatct < 1:304489‑M1/143‑79 (MQ=255) ccgcccgGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctg > 1:625960‑M1/1‑64 (MQ=255) ccgGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgttta < 2:952222‑M1/143‑84 (MQ=255) gAACTGGAAGACATCGAAGCATCGGCACGATTCTTTGAAGACGACGACGGCCTGCATctccttcttcttcttagaagatgcggaagatcacgccggtaactcccctgtggcatttaccatccgtgatggccgtctgttaacgc > 1:442384‑M1/1‑57 (MQ=255) gtgGGAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgc < 2:629839‑M1/142‑90 (MQ=255) gAAGACATCGAAGCATCGGCCCGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgcagctgcggaagatcacgccggtaactcccctgtggcatttaccatccgtgatgctcgtatggttcctctgcgtc > 1:118953‑M1/1‑51 (MQ=255) gAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaggatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtg > 2:168219‑M1/1‑51 (MQ=255) gACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagc > 1:995897‑M1/1‑48 (MQ=255) gACATCGAAGCAGCGGCACGTTGCGTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagc < 2:702431‑M1/143‑96 (MQ=255) aTCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgcgcgtg > 2:956830‑M1/1‑45 (MQ=255) aTCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgagatggtcgcctgtgtacgctgcgtgagcctg > 1:708200‑M1/1‑45 (MQ=255) gAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaac < 1:701507‑M1/143‑102 (MQ=255) gAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCACctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaac < 2:659646‑M1/143‑102 (MQ=255) gCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgc < 2:304714‑M1/143‑105 (MQ=255) gCATCGCGATGTTTCTTTGAAGACGACGGGGTCGTGCAT‑‑‑‑‑CTTCTTCTTTTTCTGTGAAGATGCGTAAGAGCACGCCCACAACACAAATGATTCTTTTACCATGCGTGATGGTCGGCTGTTTACTCTGCGTGAGCGTGAACTGc < 1:1068872/143‑1 (MQ=255) gCACTTTTCTTTGACGACGACGCCGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgctt < 1:1043767‑M1/143‑111 (MQ=255) cggttcttTGAAGGCGACGACGGGCTGCATctccttcttcttctgtgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttc < 2:73337‑M1/140‑114 (MQ=255) tttCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtgtggttactctgcgtgagcgtgaactgcccgcttttcgt > 1:323866‑M1/1‑28 (MQ=255) tttCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgggcgtgaactgcccgcttttcgt > 2:1050572‑M1/1‑28 (MQ=255) tttCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgt < 2:574899‑M1/143‑116 (MQ=255) aaGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgctgttcgtctgtatcg > 2:388510‑M1/1‑20 (MQ=255) acgacgacgGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcctttcgtcggtatcgtat > 1:1084225‑M1/1‑17 (MQ=255) gacgactGCCTGCATctacttcttcttctttgaagatgcggaagctcacgccggtaactccactttggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgc < 1:53522‑M1/143‑129 (MQ=255) ccggcggcctGCATctccttgttcttctttgacgacgcggacgatcacgccgctaacgccactgtgtcagttaccatccctgatggtcgtctgtttcctctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcg < 1:1046295‑M1/139‑130 (MQ=255) ggCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgctggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgccc < 1:579302‑M1/143‑135 (MQ=255) | AGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCG > NC_000913/4001502‑4001777 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |