| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,316,391 | T→C | Y73H (TAC→CAC) | rcsB → | response regulator in two‑component regulatory system with RcsC and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,316,391 | 0 | T | C | 95.3% | 178.3 / NA | 65 | Y73H (TAC→CAC) | rcsB | response regulator in two‑component regulatory system with RcsC and YojN |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (31/30); minor base A (1/2); total (33/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCCGAAAGC > NC_000913/2316256‑2316530 | tGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAGACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGCACGGCGATGGCATTACCATAATCAAGCACATaaa > 1:179899/1‑143 (MQ=255) gTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCACCAACCTGCCGAAACTGGATGCGCATGTGCTGATTACCGCTCCCCCCACACCTGGCCATAACTCCGGCGAACGCATCACCGTAGCCAAGCACATCCAgcg > 2:220615/1‑143 (MQ=255) gTGAATGTCGTCGCCTAATTTGAAGACTCTACACCACTGACCAACACCCTGCCGAACCTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAgcg < 2:1036811/143‑1 (MQ=255) aTGCTGTCGGCGAATTTGAAGACCCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCGGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCAt < 2:468259/143‑1 (MQ=255) tgttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCAtt > 2:465954/1‑143 (MQ=255) ttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCCAGCACATGAAGCGCCAtctc > 1:952415/1‑143 (MQ=255) ttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCCTTTc > 1:302599/1‑143 (MQ=255) tgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGTTGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTcc < 2:440966/143‑1 (MQ=255) cGGCGTATTTGAAGGCTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCaa < 1:463684/143‑1 (MQ=255) gAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCCATGGCATTACCTTAATCAAGAACATCAAACGCCACTTCCCAACCCt > 1:407226/1‑143 (MQ=255) tttGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTATTCCTGCGCATCAAGCGGCATTTCCTAAGCCTGTc > 2:10369/1‑143 (MQ=255) gAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCGTAATCAAGCACATCAAGCGCCACTTCCCAAGCCTGTCGac > 1:1072385/1‑142 (MQ=255) ctACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGtt < 2:879845/143‑1 (MQ=255) ggCACTGATCAACCACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGa < 1:348231/142‑1 (MQ=255) cACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCATGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTGGATGATTGGTCTGACt > 1:1058211/1‑143 (MQ=255) tGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg > 1:554481/1‑143 (MQ=255) tGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg > 1:1003872/1‑143 (MQ=255) tGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg < 1:326114/143‑1 (MQ=255) tGAGCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg < 1:643738/143‑1 (MQ=255) tcaacccgctggtgAAGCTGGATGTGCATGTGTTGATTATCGATCTCTCCATGCCTGGCGATAAGTACGGCGATCGCATTACTTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCAGTGTTCTGACTATGaac < 1:304915/130‑1 (MQ=255) caacaaCCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGaaca < 2:232021/143‑1 (MQ=255) aacaacCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCCCACAACGTGCCATTTCCCCCGCACGTCGATCATTGTTCTGACTATGaacaa > 1:650767/1‑143 (MQ=255) aaCTGGGCGCGCATGTGTTGATTACCGGTCTCGCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGAtt < 1:104766/143‑1 (MQ=255) tGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACACGGCGATTCtt > 1:95544/1‑143 (MQ=255) ggATGCGCATGTGTTGATTACCGATCGCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTa < 1:470490/143‑1 (MQ=255) gATGCGCATGTGTTGATTACCGTTCTCGCCATGCTTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGGTCATTGTTCTGACTATGAACAACATCCCGGCGATTCTTAg < 1:922885/143‑1 (MQ=255) tgagtagATTACCGATCTATCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATTAATCACATCAATCGCCATTATCAAAGCCTGTCGATCATTGGTCGGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTAt < 2:177434/137‑1 (MQ=255) gtgtTGATTACCGATCGCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTAtt < 2:933795/143‑1 (MQ=255) gATTACCGATCTCTCCATGCCCGGCGATAAGTACGGCGATGGCATTACCTTAACCAAGCACATCAAGCGCCATTTCCCAAGCCGGTCGATCATTGTTCTGACTATGAACAACAACCCGGCTATTCTTAGTGCGGTATTGGATc < 2:332966/143‑1 (MQ=255) tACCGCTCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCCAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTgg < 2:887169/143‑1 (MQ=255) cGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCACGAGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGata > 1:1012866/1‑143 (MQ=255) cGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGGGGTGTTGGATCTGGata > 1:985853/1‑143 (MQ=255) aTCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTACCAAGACTGTCGATCATTGTTCTGACGATGAACAACACCCCGGCGATTCTTAGTGCGGCAATGGCGCTGGatagc > 1:635802/1‑141 (MQ=255) tcgccATGCTTGGCGATAAGTACGGCGATGGCCTTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGaa < 1:66132/140‑1 (MQ=255) actcccggCCTCGCGATAAGTACGGCTATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGaa < 2:347364/136‑1 (MQ=255) tgaaTGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCGCATCAAGCGCCATGTGCCAAGCCTGTCGATCACTGTTCAGACTATGGACAAGAAGCCGGTGAGTGTTAGTGCGGAGTTGGGTCCGGATATCGCAgg > 1:584194/4‑143 (MQ=255) tGCCTGGCGATAAGTACGGCGTTGGCATAGTGGTTATCAAGAACTCCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATc < 1:10369/143‑1 (MQ=255) gCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTAGATCATTGTTCTGACTATGAACAACAACCCGGAGATTCTTAGTGCGGGATTGGAACTGGATATCGAAGGGAACg > 1:505489/1‑143 (MQ=255) tGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGc > 2:730249/1‑143 (MQ=255) tGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGc < 2:554481/143‑1 (MQ=255) ggCGAGAAGTACAGGGATGGCATGACCGAAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGTTATTGGATCTGGATATCGAAGGGATCGTGCt < 2:312420/143‑1 (MQ=255) gCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCCTTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGAATTGGACCTGGATATCGAAGGGATCGTGCTg > 1:507458/1‑143 (MQ=255) gATACGTGCGGGAATGGGATTCGCGTACTCAAGAACATGAGTCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGAGTATAAACAACAACCCGGCGATTCTTAGTGCGGTATAGGATCGGGATATCGAAGGGATCGTGCTGaa < 1:928539/143‑1 (MQ=255) aatcaGTACGGCGATGGCATTCCCTTGATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTGCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGaa < 2:329556/139‑1 (MQ=255) aaGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACCATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGCTATCGAAGGGATCGTGCGGAAACa > 1:609011/1‑143 (MQ=255) aaGTACGGCGATGGCATTAACTTAAACAAGGCCATCAAGCGCCATTGGCCAAGCCTGTCGATCATTGTTATGACTATGAACAACAACCCGGCGACTCTTAGTGCGGTATTGGATCTGGCTATCGAAGGGATGGTCCTGAAACa > 1:667028/1‑143 (MQ=255) aGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGACACaa > 1:740958/1‑143 (MQ=255) ggaCGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAg < 2:861501/141‑1 (MQ=255) gTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAg < 2:512082/143‑1 (MQ=255) tACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGATCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAgg > 1:338892/1‑143 (MQ=255) acctttaTGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGt < 2:547103/137‑1 (MQ=255) ggCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGc > 1:812921/1‑143 (MQ=255) tgttgattgttccTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCac < 2:546986/132‑1 (MQ=255) aTGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGCTCGTGCTGAAACAAGGTGCaccg > 2:999964/1‑143 (MQ=255) aTGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccg > 2:1075824/1‑143 (MQ=255) ggCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccgac < 2:1006305/143‑1 (MQ=255) gCATTACCTTAATCAGGCACAGCAAGCGCCATTCCCCAGGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccgacc < 1:987952/143‑1 (MQ=255) gCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGACACACGGTGCcccgacc > 2:679460/1‑143 (MQ=255) cATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAATCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccgaccg < 2:407226/143‑1 (MQ=255) aTTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTCTTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCAccgaccga > 1:773198/1‑143 (MQ=255) cTTAATCCAGCACATCCAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGTCGATTTTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGc < 2:98840/143‑1 (MQ=255) cTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGCCTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATACCGAAGGGATCGTGCTGAAACAGGGTGCACCGACCGATCGGc > 2:596060/1‑143 (MQ=255) tCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGGCCGAGCTGCCGaaa > 2:167545/1‑143 (MQ=255) aaGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGCACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGGTATCGACGGGATCGTGCTGAAACCAGGTGCATCGGCCGTGCTGCCCaagcc > 2:90127/1‑140 (MQ=255) aaGCACAGCAAGCGCCATTTCCCAAGCCTGTCGATCATTGATCTGACGATGAACAACAACCCCGCGAGTCGTAGCGCGGCATTGGATCTGGAACCCCAAGCGAGCGTCCTCAAACCACGAGCACCGAGCGACCACCCGaagcc > 1:39295/1‑140 (MQ=255) | TGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCCGAAAGC > NC_000913/2316256‑2316530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |