Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,308,102 G→A A274V (GCA→GTA)  nlpI ← lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,308,1020GA100.0% 158.9 / NA 51A274V (GCA→GTA) nlpIlipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/24);  total (27/24)

TTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGC  >  NC_000913/3307964‑3308239
                                                                                                                                          |                                                                                                                                          
ttAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCCGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTa                                                                                                                                        >  1:45767/1‑143 (MQ=255)
   aCGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATAACGGGATGATGTGTACGTCAGAGTAGCCGGGGCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCg                                                                                                                                     >  2:1093101/1‑143 (MQ=255)
                 ccTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGCGCGATAATTCCAAGAATACGTATCgggggccgacacagg                                                                                                                       >  1:908760/1‑130 (MQ=255)
                 ccTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGt                                                                                                                       >  1:852710/1‑143 (MQ=255)
                 ccTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGCCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGt                                                                                                                       >  1:768158/1‑143 (MQ=255)
                    cACCCGTTAAGGTGATGGCAATCACAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTAt                                                                                                                    <  1:417177/143‑1 (MQ=255)
                            gggggtgtGGCAATCAGAAGAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACgttg                                                                                                            <  1:316102/136‑1 (MQ=255)
                              ggTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACgttgtt                                                                                                          <  2:605424/143‑1 (MQ=255)
                                     gCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCaagc                                                                                                   >  2:159309/1‑141 (MQ=255)
                                       aagcataacaGATTACGGGCTCATGTCTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGTCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGc                                                                                                 <  1:468291/134‑1 (MQ=255)
                                        aTCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGcc                                                                                                <  2:732194/143‑1 (MQ=255)
                                         ttaaaaaaGATTACGGGCTGATGTATACGTCAGCTATTGCTGGTCCGATTCTGCCAGTTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCa                                                                                               <  1:893749/141‑1 (MQ=255)
                                             aaaaGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGttt                                                                                           >  2:595602/1‑143 (MQ=255)
                                                      cGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGc                                                                                  <  1:1078999/143‑1 (MQ=255)
                                                          cTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTg                                                                              <  2:836243/143‑1 (MQ=255)
                                                            gATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCATCAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgc                                                                            <  1:636695/143‑1 (MQ=255)
                                                            gATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCCCAACAAAGTTATGAACGATGTTGGCAACCGCCAGTCTCAACAGTGTCATGgc                                                                            >  1:169187/1‑143 (MQ=255)
                                                             aTGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCCTCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGGAACCCCCAGTTTGAACCGTGCCGTGgcg                                                                           >  2:957034/1‑143 (MQ=255)
                                                             aTGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTTGCAACCGCCAGTTTGAACCGCGCCGTgggg                                                                           >  2:111382/1‑141 (MQ=255)
                                                                  tACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTc                                                                      >  2:490360/1‑143 (MQ=255)
                                                                           tcttGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATcccc                                                             <  2:496717/141‑1 (MQ=255)
                                                                           tATTGCTGGTCCGATTCTGCCAGATCATCTTGGTCCTGGCCCAGGAGAGATAATACCAACAATAAATAACGGTGCTCCAAAAAGTTATGAACGGTTTGAGCAACCGCCAGCTGAAACAGTGAGGCAGCGCTGGCAAAAAccca                                                             >  1:289713/1‑142 (MQ=255)
                                                                              tGCCGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTAAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAg                                                          <  2:185214/143‑1 (MQ=255)
                                                                                cTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGAc                                                        >  2:898391/1‑143 (MQ=255)
                                                                                cTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCAGTGCTCAACAAAGTAATGAACGTTGTTGGCAACCGCCAGGTTGAACAGTGCCGTGGCGCTGGCCAAGTCCCCCAgcc                                                        >  2:956705/1‑141 (MQ=255)
                                                                                 tGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTGGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACt                                                       >  2:93551/1‑143 (MQ=255)
                                                                                    tCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACACAGTTATGAACGTTGTTGGAAAACGACAGAGTAAACAATGCTTGAGCGATGTCAAAATCCCCCAGACTGAg                                                    >  1:177039/1‑143 (MQ=255)
                                                                                    tCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAg                                                    >  1:136550/1‑143 (MQ=255)
                                                                                    tCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAg                                                    <  1:1093101/143‑1 (MQ=255)
                                                                                    tCCGACTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAg                                                    <  1:982555/143‑1 (MQ=255)
                                                                                    cccGCTTCTGCCCGGTCATCTTGGTCCTGTCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAg                                                    <  2:1020322/142‑1 (MQ=255)
                                                                                       gATGCTGCCAGGTCATCTTGGGCCGGGCCCAGGAGGGATCCGTCGAACAGTACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGgt‑a                                                <  1:227562/143‑1 (MQ=255)
                                                                                         ttCTGCCAGGTCATCTTGGTCCTGGCCAAGGAACGATAATTGCAACAATACGTATCGGTGCTCAACAAATTTATGAACGTTGTTGGCAACCGCCAGTTTGAAAAGTGACTGGGCGATGTCCAAATGCGCCAGACTTAGgt‑agt                                              >  1:455068/1‑143 (MQ=255)
                                                                                          tCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGgt‑agta                                             <  2:67345/143‑1 (MQ=255)
                                                                                          tCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCACTTTGAACAGTGCCGTGGCGCTCTCCAAATCCCCCACACttccgcaaca                                              >  1:764740/1‑135 (MQ=255)
                                                                                               cAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTAc                                        >  2:544924/1‑143 (MQ=255)
                                                                                               cAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGCTTCAACAGTGTTGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACttgc                                        >  2:203612/1‑141 (MQ=255)
                                                                                               cAGGTCATCGTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGTAAGTACtac                                         <  1:577695/143‑3 (MQ=255)
                                                                                                aGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTTTTGGCAACCGCCAGTTGAAACAGTGCGGGGGCGCGTTGCACATCGCACAGCGTTCGGT‑AGTACtgccc                                       >  1:876294/1‑139 (MQ=255)
                                                                                                 gggcATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTAGCGGTGCTCAACAAAGGAATGAACGTGGTTGGCAACCGGCGGGTTGAACAGTGCCGTGGCGCTGTCCAACTCCCCCAGCCCTAGGT‑AGtacctaccg                                      >  1:469960/4‑142 (MQ=255)
                                                                                                  gTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTa                                     >  2:330529/1‑143 (MQ=255)
                                                                                                              cTGGCCCAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTgg                         <  2:358592/143‑1 (MQ=255)
                                                                                                                   ccAGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTATTGGCAACCGCCCGTTTGCACAGTGCCGTGGCGCTGCCCAAATCCCCCCGACTTAGGT‑AGTACTTACCTGAATCGATGTAGGTTTcc                    >  1:102007/1‑142 (MQ=255)
                                                                                                                     aGGAGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACt                  <  2:852710/143‑1 (MQ=255)
                                                                                                                        aGCGATAATTCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTgag               <  2:363670/143‑1 (MQ=255)
                                                                                                                             tgatgcccacaaTAGGTAGCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGCCGTGGGT‑AGTGATTTCGGATATAGAAGTTGGTTTCACTGAGATGCt          <  1:81233/135‑1 (MQ=255)
                                                                                                                                 tCCAACAATACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGTACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCagc      <  2:750164/143‑1 (MQ=255)
                                                                                                                                   caacaaTACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCagcga    >  2:259515/1‑143 (MQ=255)
                                                                                                                                   caacaaTACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACATAAATAGAAGTTGGTTTCACTGAGATGCTCagcga    >  2:422909/1‑143 (MQ=255)
                                                                                                                                    aacaaTACGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCagcgag   <  2:66320/143‑1 (MQ=255)
                                                                                                                                     acaaTACGTATCGGTGCTAAACAAAGTTATGAACGTTGTGGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTcagccagc  <  1:1023632/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCAAATCCCCCAGACTTAGGT‑AGTACTTACCTAAATAGAAGTTGGTTTCACTGAGATGCTCAGCGAGC  >  NC_000913/3307964‑3308239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑