| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,103,655 | Δ1 bp | coding (1320/1374 nt) | cpxA ← | sensory histidine kinase in two‑component regulatory system with CpxR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,103,653 | 0 | G | . | 97.1% | 138.9 / ‑2.6 | 35 | coding (1322/1374 nt) | cpxA | sensory histidine kinase in two‑component regulatory system with CpxR |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (19/15); minor base A (1/0); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGA > NC_000913/4103513‑4103790 | tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCggc > 1:546333/1‑142 (MQ=255) aCGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCATATCCTGCCTGCAAATGCGAAGGTTAACTCCGCTTATACAGCGGCAACCAACGCACCAGCCGCCACCCGACCAGCggctagactc > 2:254141/1‑135 (MQ=37) gCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAACTGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTg > 1:574289/1‑143 (MQ=255) tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGTGTAGATGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCACTTATACAGCGGCAACCAAATAACCAGCCGTAAACCGCCCAG‑AGGCTGTATTCTGcc > 1:332535/1‑143 (MQ=38) tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCCGCCGTAAACCGCCCAGC‑GGCTGCCCTCTGcc > 1:563373/1‑143 (MQ=255) tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGGAAACGCCTTATCCTGCATGTGGGCACCAAACAAAACTCCCGCCAACCGGCTCCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGcc < 1:364182/143‑1 (MQ=12) cggcGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCAcc < 1:724612/140‑1 (MQ=255) aGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCGGCCGTAACCCACCCAGC‑GGCTGGCTTCTGCCTGCACCCAGCCACGa > 1:303910/1‑143 (MQ=38) cATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAGATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTCCTGCCTTCAGCCAGCCACGAcgc > 2:369414/1‑143 (MQ=39) aTCCTGCTCAAATGCCGGAGGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGAtgct < 1:644171/143‑1 (MQ=255) tCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTCACTCCGCTTATACAGCGGCAACCAACTCCCCAGCCCTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGAtgctg > 1:491785/1‑143 (MQ=39) tCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGAtgctg > 1:653378/1‑143 (MQ=255) ccTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAGCTCGCCAGCCGTCAACCGCCCAGC‑AAATGTCTTCAGCATTCAGTCAGACACGAtacaac > 1:36670/1‑138 (MQ=25) tGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAAATCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCAAGC‑GGCTGTCCTCTGCCTCCACCCAGCCCCGCtgctgcgc > 2:391077/1‑141 (MQ=38) gCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCCTCACCCAGCAACGACGCCTCTGa > 2:120156/1‑143 (MQ=39) gCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCATGCAAATGCGAAGTTTAACTCGGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGGtgctgcggg > 1:706022/1‑140 (MQ=255) aaaTGCCGGATGCGGCGTACACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATgg < 2:783202/143‑1 (MQ=255) aTGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCg > 1:551807/1‑143 (MQ=255) gcgTAAACGCCTTATCCCGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATc < 1:458118/143‑1 (MQ=255) ccTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGACGCCGCTGACTGGCGGCTTCAACAACCGCCACcccc > 2:332724/1‑143 (MQ=255) tgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCCGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACACGCCCCAGCCCCAAACCtccaccc > 2:394905/1‑137 (MQ=255) cAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTAACCCAGCCACGATGATGCTGAATGGCGGTTTCAACAAGCGCCAGCCCACAAGCAGTACAGcc > 2:11237/1‑143 (MQ=255) gAAATTTAACTCCGCTTATACAGCGGCAACAAAATTACCAGACGTAAACCGCACAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCACACCTGTACCGCCAGATTCa > 2:685393/1‑143 (MQ=255) ggtAACTCCGCTTATACAGCGGCAACCAGATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGAt < 1:383756/141‑1 (MQ=255) cTCCGCTTATCCAGCGGCACCCAACTCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGt < 2:446837/143‑1 (MQ=255) tataCAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACACTCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCAt > 2:142832/1‑143 (MQ=255) atacgccgccaaccaacTCACCAGCCGTATACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATc < 2:457181/134‑1 (MQ=255) taCAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCg < 1:477737/143‑1 (MQ=255) cGGCAACCAAATCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTCAATGCCGGTTTCAACACTCGCCAGCCCCAACCCTGTCCCGCCCGACTCCCGTTCGCGTCCTCCATCGGTACg > 2:113995/1‑143 (MQ=255) aaCCAGCTCACCAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAg < 2:441318/143‑1 (MQ=255) aaCCAATTCCCCCGCCGTAAACCGCCCAGC‑CGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAg < 2:438225/143‑1 (MQ=255) cAGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacggacggca > 1:473355/1‑141 (MQ=255) aGCCGTAAACCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATCGAACGGACGGaac > 1:97448/1‑142 (MQ=255) aaCCGCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGtt < 1:399817/143‑1 (MQ=255) ccGCCCAGC‑GTCTGTCTTCTGCCTGCACCCAGCCACGATGCTGCCGAACTCCCCATACAACCATCGCCAGCCCCAAACCTGTACCGGCAGAGTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTcg < 2:512645/143‑1 (MQ=255) gCCCAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTcgcg < 1:116876/143‑1 (MQ=255) cccAGC‑GGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGa < 1:114043/143‑1 (MQ=255) | TGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGA > NC_000913/4103513‑4103790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |