Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,103,655 Δ1 bp coding (1320/1374 nt) cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,6530G.97.1% 138.9 / ‑2.6 35coding (1322/1374 nt)cpxAsensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (19/15);  minor base A (1/0);  total (20/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGA  >  NC_000913/4103513‑4103790
                                                                                                                                            |                                                                                                                                         
tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCggc                                                                                                                                         >  1:546333/1‑142 (MQ=255)
       aCGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCATATCCTGCCTGCAAATGCGAAGGTTAACTCCGCTTATACAGCGGCAACCAACGCACCAGCCGCCACCCGACCAGCggctagactc                                                                                                                                  >  2:254141/1‑135 (MQ=37)
         gCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAACTGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTg                                                                                                                               >  1:574289/1‑143 (MQ=255)
           tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGTGTAGATGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCACTTATACAGCGGCAACCAAATAACCAGCCGTAAACCGCCCAGAGGCTGTATTCTGcc                                                                                                                             >  1:332535/1‑143 (MQ=38)
           tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCCGCCGTAAACCGCCCAGCGGCTGCCCTCTGcc                                                                                                                             >  1:563373/1‑143 (MQ=255)
           tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGGAAACGCCTTATCCTGCATGTGGGCACCAAACAAAACTCCCGCCAACCGGCTCCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGcc                                                                                                                             <  1:364182/143‑1 (MQ=12)
                 cggcGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCAcc                                                                                                                       <  1:724612/140‑1 (MQ=255)
                          aGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCGGCCGTAACCCACCCAGCGGCTGGCTTCTGCCTGCACCCAGCCACGa                                                                                                              >  1:303910/1‑143 (MQ=38)
                             cATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAGATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTCCTGCCTTCAGCCAGCCACGAcgc                                                                                                           >  2:369414/1‑143 (MQ=39)
                              aTCCTGCTCAAATGCCGGAGGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGAtgct                                                                                                          <  1:644171/143‑1 (MQ=255)
                               tCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTCACTCCGCTTATACAGCGGCAACCAACTCCCCAGCCCTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGAtgctg                                                                                                         >  1:491785/1‑143 (MQ=39)
                               tCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGAtgctg                                                                                                         >  1:653378/1‑143 (MQ=255)
                                ccTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAGCTCGCCAGCCGTCAACCGCCCAGCAAATGTCTTCAGCATTCAGTCAGACACGAtacaac                                                                                                        >  1:36670/1‑138 (MQ=25)
                                  tGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAAATCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCAAGCGGCTGTCCTCTGCCTCCACCCAGCCCCGCtgctgcgc                                                                                                      >  2:391077/1‑141 (MQ=38)
                                   gCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCCTCACCCAGCAACGACGCCTCTGa                                                                                                     >  2:120156/1‑143 (MQ=39)
                                   gCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCATGCAAATGCGAAGTTTAACTCGGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGGtgctgcggg                                                                                                     >  1:706022/1‑140 (MQ=255)
                                       aaaTGCCGGATGCGGCGTACACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATgg                                                                                                 <  2:783202/143‑1 (MQ=255)
                                         aTGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCg                                                                                               >  1:551807/1‑143 (MQ=255)
                                                     gcgTAAACGCCTTATCCCGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATc                                                                                   <  1:458118/143‑1 (MQ=255)
                                                              ccTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGACGCCGCTGACTGGCGGCTTCAACAACCGCCACcccc                                                                          >  2:332724/1‑143 (MQ=255)
                                                                          tgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCCGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACACGCCCCAGCCCCAAACCtccaccc                                                              >  2:394905/1‑137 (MQ=255)
                                                                            cAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTAACCCAGCCACGATGATGCTGAATGGCGGTTTCAACAAGCGCCAGCCCACAAGCAGTACAGcc                                                            >  2:11237/1‑143 (MQ=255)
                                                                                   gAAATTTAACTCCGCTTATACAGCGGCAACAAAATTACCAGACGTAAACCGCACAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCACACCTGTACCGCCAGATTCa                                                     >  2:685393/1‑143 (MQ=255)
                                                                                       ggtAACTCCGCTTATACAGCGGCAACCAGATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGAt                                                 <  1:383756/141‑1 (MQ=255)
                                                                                            cTCCGCTTATCCAGCGGCACCCAACTCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGt                                            <  2:446837/143‑1 (MQ=255)
                                                                                                   tataCAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACACTCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCAt                                     >  2:142832/1‑143 (MQ=255)
                                                                                                    atacgccgccaaccaacTCACCAGCCGTATACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATc                                    <  2:457181/134‑1 (MQ=255)
                                                                                                     taCAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCg                                   <  1:477737/143‑1 (MQ=255)
                                                                                                          cGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTCAATGCCGGTTTCAACACTCGCCAGCCCCAACCCTGTCCCGCCCGACTCCCGTTCGCGTCCTCCATCGGTACg                              >  2:113995/1‑143 (MQ=255)
                                                                                                              aaCCAGCTCACCAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAg                          <  2:441318/143‑1 (MQ=255)
                                                                                                              aaCCAATTCCCCCGCCGTAAACCGCCCAGCCGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAg                          <  2:438225/143‑1 (MQ=255)
                                                                                                                         cAGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacggacggca               >  1:473355/1‑141 (MQ=255)
                                                                                                                          aGCCGTAAACCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATCGAACGGACGGaac              >  1:97448/1‑142 (MQ=255)
                                                                                                                                 aaCCGCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGtt       <  1:399817/143‑1 (MQ=255)
                                                                                                                                   ccGCCCAGCGTCTGTCTTCTGCCTGCACCCAGCCACGATGCTGCCGAACTCCCCATACAACCATCGCCAGCCCCAAACCTGTACCGGCAGAGTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTcg     <  2:512645/143‑1 (MQ=255)
                                                                                                                                     gCCCAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTcgcg   <  1:116876/143‑1 (MQ=255)
                                                                                                                                      cccAGCGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGa  <  1:114043/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGA  >  NC_000913/4103513‑4103790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: