Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,316,391 T→C Y73H (TAC→CAC)  rcsB → response regulator in two‑component regulatory system with RcsC and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,316,3910TC100.0% 136.8 / NA 50Y73H (TAC→CAC) rcsBresponse regulator in two‑component regulatory system with RcsC and YojN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/18);  total (32/18)

AGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCCGAAA  >  NC_000913/2316254‑2316528
                                                                                                                                         |                                                                                                                                         
aGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTATCCATACCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATc                                                                                                                                      >  1:219412/1‑143 (MQ=255)
     gTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAgcg                                                                                                                                 >  1:414352/1‑143 (MQ=255)
      tGAATGTTGTCGGCGAATTTGAAGACGCGACAGCACTGATCAACCTCCTGCCGAAACTGGATGCGCATGTGTGGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAgcgc                                                                                                                                <  2:751688/143‑1 (MQ=255)
        aaTGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGATACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAACGccc                                                                                                                              >  2:418359/1‑142 (MQ=255)
            ttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGGAACTGGATGCGCATGTGTTGAAGACCGACCGCTCCATGCATGCCGATAAGTACGGCGATGGCATTACCTCAAGCAGGCGCAGCAAGCGCCACTTc                                                                                                                          >  2:51451/1‑143 (MQ=255)
            ttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTc                                                                                                                          <  1:419603/143‑1 (MQ=255)
                 ggCGAATTTGAAGACTTTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAg                                                                                                                     <  2:716212/143‑1 (MQ=255)
                  gCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGCTGGCATTACCTTAATCAAGCACATCACGCGCCATTTCCCAAGc                                                                                                                    >  2:542463/1‑143 (MQ=255)
                  gCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGc                                                                                                                    >  1:253619/1‑143 (MQ=255)
                   cGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCACGcc                                                                                                                   >  1:320461/1‑143 (MQ=255)
                    gAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCt                                                                                                                  >  2:579488/1‑143 (MQ=255)
                      aTTTGAAGACTCGACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGt                                                                                                                <  2:180343/143‑1 (MQ=255)
                              acgctACAGCACTGATCAAGAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCAtt                                                                                                        <  1:222464/140‑1 (MQ=255)
                                tctACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGAATGACCCTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGt                                                                                                      >  1:570940/1‑143 (MQ=255)
                                tctACAGCACTGATCAACAACCTGCCAAAACTGGATGCGCATGTGTTGATGACCGATCTCTCCATGCCAGGCGATAAGTACGGCGATGACACTACCTTAATCACGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCAtggc                                                                                                      >  2:72385/1‑140 (MQ=255)
                                 ctACAGCACGGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGtt                                                                                                     <  1:295505/143‑1 (MQ=255)
                                  tACAGCGCTGATCAAGAACGTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGAGCCATTTCCCAAGCCTGTCGATCATTGTTc                                                                                                    <  2:280169/143‑1 (MQ=255)
                                   aCAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGGTAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCt                                                                                                   >  1:416894/1‑143 (MQ=255)
                                   aCAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATACGTACGGCGCTGGCATTACCTTAATCAAGCACATCAGGCGCCATTTCCCAAGCCTGTCGAGCCTTGTTCt                                                                                                   >  2:102430/1‑143 (MQ=255)
                                        aCTGATCAACACCCTGCCGAGACTGGATGCGCATCTGTTGATTACCGCTCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTa                                                                                              <  2:518977/143‑1 (MQ=255)
                                        aCTGATCAAAAGCCGGCCGAAACGGGATGCGCATGGGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTa                                                                                              <  2:247163/143‑1 (MQ=255)
                                          tgacccacagcCCGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTCCCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg                                                                                            <  2:33870/137‑1 (MQ=255)
                                            aTCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGCCGATCATTGTTCTGACtctcaa                                                                                          >  2:81446/1‑138 (MQ=255)
                                                         cGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCg                                                                             >  2:18962/1‑143 (MQ=255)
                                                          gAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCCCCTCAGGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAATCCGgaga                                                                            >  1:91781/1‑143 (MQ=255)
                                                          gAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCCAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGAAAAAAAACAACAACACGGCGa                                                                            >  1:448242/1‑143 (MQ=255)
                                                            aaCTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGCTGGCCTTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGAta                                                                          >  1:494833/1‑142 (MQ=255)
                                                             aCTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTACTCACGCACCTCCAGCGCCATTTCCCCAGCCTGTCGATCATTGTTCTGCCTACGAACAACAACCCGGCGAtcc                                                                         >  2:251883/1‑141 (MQ=255)
                                                                ggATGCGGATGTGTTGATCACCGATCGCTCCATGCCTGGCGATAGGTACGGCGATGGCATTACCTTAACCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTa                                                                      <  1:144856/143‑1 (MQ=255)
                                                                  aTGCGCATGTGTTGATTACCGATCTCTCCATGTCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCAAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCcgacgacactcatt                                                                    >  2:71532/1‑135 (MQ=255)
                                                                   ggcgcGTGTGTTGATGACCGCTCGCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTAGCTTCATCAAGCACATCGAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTg                                                                   <  2:320705/142‑1 (MQ=255)
                                                                      gcATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCCGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTAAGTGCgg                                                                >  1:630062/1‑143 (MQ=255)
                                                                          gtgtTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTAtt                                                            <  2:317741/143‑1 (MQ=255)
                                                                           tgtTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAGGCCGGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTg                                                           >  1:292068/1‑143 (MQ=255)
                                                                              tGATTACCGATCTCTCCATGCCTGGCGATAAGTAAGGCGATGGCATTACGTGAATCAAGCAGATCAAGCGCCAATTGCCAAGCCTGTCGATCATTGTGCTGACTATGAACAACGAACCGGGGATTCTTAGTGCGGTATTggct                                                        >  1:301132/1‑141 (MQ=255)
                                                                                 ttACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGACATTACCTTAATACTGCATATCTTGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTg                                                     <  1:780026/143‑1 (MQ=255)
                                                                                    ccGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACAGCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACGACAACCCGGCGATTCTTAGTGAGGTATGGGGTCTGGat                                                  >  2:284355/1‑143 (MQ=255)
                                                                                     cGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTCTTGGATCTGGgta                                                 >  1:344288/1‑143 (MQ=255)
                                                                                       aTCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACCACACACCGGCGATTCTTAGTGCGGTATTGGATCAGGGTATc                                               >  2:714774/1‑143 (MQ=255)
                                                                                          tctcCATGCCTGGCGATAAGTACGTCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGaa                                            <  1:261736/143‑1 (MQ=255)
                                                                                          tctcCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCGTTGTTGTAACTATGAACAACAACCCGGCGCTTCTTAGTGCGGTATTGGCTCTGGAGACCGaa                                            >  2:700443/1‑143 (MQ=255)
                                                                                              cATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGa                                        >  2:530161/1‑143 (MQ=255)
                                                                                                tGCCTGGCGATAAGTTCGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACACCCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATc                                      <  1:579488/143‑1 (MQ=255)
                                                                                                 gCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGAGCATTGTTCTGACTATGAACAACAACCCGGCGCTTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCg                                     >  1:582521/1‑143 (MQ=255)
                                                                                                              tACGGCGATGGCATTCCCTTAATCAAGCACATCAAGCGCCATTTCCCAAACCTGTCGCTGATTGTTCAGAATATGAACAACAACCAGGCGATTCTTCGTGCGGTATTGGAACTGGATATCAAAGGGATCGGGCGGaagaacgg                        >  2:663452/1‑137 (MQ=255)
                                                                                                                cGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACACggcg                      >  1:764343/1‑141 (MQ=255)
                                                                                                                         cATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccgaccg             <  2:486433/143‑1 (MQ=255)
                                                                                                                         cATTACCTTAATCAAGCACATAAAGAGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGAGATTCTTAGTGCGGTATTGGATCCAGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccgaccg             <  2:626847/143‑1 (MQ=255)
                                                                                                                               cTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGACGATTCTTAGTGCGGTATTGTAACTGAATATCGAAGGGAACGTGCTGAAAAAAGGTGAACCGACCGATCTGc       >  2:70319/1‑143 (MQ=255)
                                                                                                                                    tCAAGCACATCAAGCGCCATTTCCCAAGCATGTCGATCATTGTTCTGACTATGAACAACAACACGGCGAATCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAACCAAGGTGCACCGCCCGATCTGCCGaaa  >  2:459742/1‑143 (MQ=255)
                                                                                                                                         |                                                                                                                                         
AGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCCGAAA  >  NC_000913/2316254‑2316528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: