Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,396,911	G→A	22.8%	T177I (ACC→ATC)	nuoH ←	NADH:ubiquinone oxidoreductase, membrane subunit H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,396,911	0	G	A	22.8%	33.1 / 2.1	17	T177I (ACC→ATC)	nuoH	NADH:ubiquinone oxidoreductase, membrane subunit H
Reads supporting (aligned to +/- strand):  ref base G (7/6);  new base A (2/2);  total (9/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCA  >  NC_000913/2396654‑2397160                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          ggCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGCACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCATGCTCGGCTTCCGGCTGGTAAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACCTGCACCTGCCTGTTGACGATGTCGGTCATGTTGAATGAAACg                                                                                                                                                                                                                                          >  2:157010/1‑275 (MQ=255)    cTTGCCAACACCCGAAGAACAGCGTAACCATAAATGCAGGGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGc                                                                                                                                                                                                                                        <  1:117457/274‑1 (MQ=255)       gccagccaCCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTAAAACGGGTGACGGTGACATCCCGCCACGCACGCGATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGCCGATGTCGATCATGTTGAATGAACCGGCCTg                                                                                                                                                                                                                                     >  2:62077/1‑274 (MQ=255)                 gaaagaCAGCTGCACCAGCAAGGCAGAGAAGGTCACAATCCCGATGTACTCACCCACAAAGAACAGGCCGAACTTCATACCGGAAGATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTGCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACg                                                                                                                                                                                                                           <  1:172352/269‑1 (MQ=255)                                                               tACTCACCCACGAAGAACAGACCGAACTTCCTACCGGAATATTCAAGGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTgcg                                                                                                                                                                             <  1:100530/274‑1 (MQ=255)                                                                 cTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATAAAACCAAAGAATTACGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGATCATGTTGAATGAACCGGCCTCCGCCCCCACGCCCATCACGGAAAGCCGGGAGAAAACCCCGTAGCTCAGGGTCTgcgacc                                                                                                                                                                          >  1:23196/1‑272 (MQ=255)                                                                     acacagaagacAAGACCGAACTCCATACCCGAATATTTAAGGTGGTAGCCGTCCGCCAGTTCCTGCTCGGCTTCCGGCGGGTCAAAACGGTGACGGTGACGTACCGCCACGCCCGCGATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGc                                                                                                                                                                      <  1:207960/270‑1 (MQ=255)                                                                                                 atagtcgacagagggggaaaCCATCCGCAACTTCTTGCTCGGCTTCCGGCTTGTCAAACGGGTGACGGTGACATACCCACACGCCCGCGATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGATCAGGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGTTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTAttt                                                                                                                                           <  2:52489/256‑1 (MQ=255)                                                                                                                   aaCCATCCGCCAGGGCCTGACGGGCTTCGGGCTGGGAAAACGGGGGACGGTGACATACCGCCACGCCCGGGATGGCAAAGGTAAGAAAACCAAAGAATTGCGGGATAACGTGCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGccc                                                                                                                        <  1:62076/275‑1 (MQ=255)                                                                                                                       aTCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCCGGGTCTGCGCAGAAGCACGCATCGCACCCACCAACGAGAATTTGTTGTTACTTgctgtcccttat                                                                                                                     >  1:52489/1‑263 (MQ=255)                                                                                                                         ccGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGAAGGTGACATACCGCCACGCCCGCGATGGCAAAGGCAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGAGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCCCGCCCATCAAGGCAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGACGCATGCATCGCACCAAGCAACGAGCATTTGTTGTTACTTGGCCCGACCGCaaa                                                                                                                   >  2:51164/1‑274 (MQ=255)                                                                                                                                                                            cccccccGCCCGCGATGGAAAAGTTAATAAAACCAAAGAAGTGCGGGAGATCGTTCCACCCATGCGCCCGGCTGTTGACGATGTCGATCATGTTGCATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGCGTCTGCGCAGAATCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCg                                                               <  2:23196/269‑1 (MQ=255)                                                                                                                                                                                        cgATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACt                                                                                                                                                                                                >  1:81212/1‑134 (MQ=255)                                                                                                                                                                                            ggCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGt                                                                                                                                                                                            <  2:81213/134‑1 (MQ=255)                                                                                                                                                                                              cAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGAATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCACAAACCACCCCCAAAACCGCCAGACCAGCCCACACCAGGAAAAAAAAAAACCCGGAGATCAGGTCGGcc                                                >  1:136667/1‑271 (MQ=255)                                                                                                                                                                                                                                    cacacaTGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCACCCCGCACTGACTGGCCCAATCGCAAAGGCcag       >  1:130257/1‑275 (MQ=255)                                                                                                                                                                                                                                         aTGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCcagcagca  >  1:155097/1‑275 (MQ=255)                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          GGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATAAAACCAAAGAATTGCGGGATAACGTTCCACACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCA  >  NC_000913/2396654‑2397160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑