Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,631,846 A→T 6.8% Q371H (CAA→CAT slt → lytic murein transglycosylase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,631,8460AT 6.8% 186.9 / 4.8 59Q371H (CAA→CATsltlytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand):  ref base A (21/34);  new base T (2/2);  total (23/36)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

TGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTT  >  NC_000913/4631575‑4632113
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                           
tgggcgATATCGTCGCCTGGGGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAAGGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAAc                                                                                                                                                                                                                                                                            <  1:153279/270‑1 (MQ=255)
 gcgcGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGTCGTTACTGTCAGGCGGATTTATTGCTGGAAAGCGGACGTGAAGCTGAAGCATAAGAGGCATTGCATCCACTCa                                                                                                                                                                                                                                                                         >  2:142766/1‑275 (MQ=255)
  cgcgATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTAATGGCAGGCGGGATTATTGCTGGAACGGGGGCGGGAAGCTGAAGCAAAAGAGATTTTGCATAAACTCAt                                                                                                                                                                                                                                                                        >  1:3632/1‑275 (MQ=255)
           gtcgCCTGGCGTTTGATGGGCAACTATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGc                                                                                                                                                                                                                                                                <  1:77746/274‑1 (MQ=255)
                 tGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTTAAGCTGAAGCAAAAGGGATTTTGCAACAACTCATGCACCAGCGTGGttt                                                                                                                                                                                                                                                         >  2:104343/1‑275 (MQ=255)
                   gCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCCCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGGGGAAGGGAAGGTGAAGCACAAGAGATTTTTGATCAACTCAttgcacagacgggtttct                                                                                                                                                                                                                                                       >  2:1070/1‑258 (MQ=255)
                           tGGGCAACGATGTACCCGGCGAGCAGGCGAAATGGCGCGAGTACGCCATTATGCGCTCGCAATCTACGTCGCTTATTGAACGCCGTGTACGGATGGCGCTTGGCACCGGCGATCGTCGCGGCTTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGAt                                                                                                                                                                                                                                                <  2:131905/274‑1 (MQ=255)
                           gaggCGACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCGTATGGGACGCCGTGTACGAATGGCGCGTGGCCCCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGAt                                                                                                                                                                                                                                                <  2:2205/272‑1 (MQ=255)
                                         aCCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGAATCAACTCCTGCAACCGCGTGGTTTCTACCCGATGGTTGCAGCACAACg                                                                                                                                                                                                                                 >  1:45100/1‑275 (MQ=255)
                                            gacgaGCAGGCGAAATGGCGCGATGACGCGATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGGCGTGGCCTGAATACATGGCTGTCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCa                                                                                                                                                                                                                               <  2:3632/274‑1 (MQ=255)
                                             acgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCCATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTCTGGAAGCGAAAGAGAAAGCTGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGGACGCGGACGTGAAGCTCAAGCAAAAGAGATTTTGCATCAACTCATGCACCAGCGTCGTTTCTACCCGCTGGTTGCAGCACAACGCATc                                                                                                                                                                                                                             >  2:110609/1‑275 (MQ=255)
                                              cgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACCCATc                                                                                                                                                                                                                             >  2:235708/1‑274 (MQ=255)
                                                  acggCGAAATGGCGGGAGGACGCCATTATGCGCTCGCAATCTACTGCGCTTATTGAACGCCTTGTACGAATGGCGCTTGGCCCCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCg                                                                                                                                                                                                                         <  1:1070/272‑1 (MQ=255)
                                                                 gATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGACGCGGCCTGAATACCTGGCTGGCGCGTCTGACTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTg                                                                                                                                                                                                           <  2:58194/273‑1 (MQ=255)
                                                                                   tCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGcgccgc                                                                                                                                                                                        >  2:77423/1‑274 (MQ=255)
                                                                                     gCAATCTACTCCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAg                                                                                                                                                                                      >  2:57355/1‑274 (MQ=255)
                                                                                               gcGCTGATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGTCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGGAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACa                                                                                                                                                                            <  2:83709/273‑1 (MQ=255)
                                                                                                   ttATTGAACCCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCTGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAACGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGAATTGAAGCTGAAGCAAAAGAGATTTTGCATCGACTCCTTCAAAAGCGTTGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGCACGGTATGAGCTGAAGGTTGATAAAGCGCCGAACAACGTTGACAgagc                                                                                                                                                                        >  1:192394/1‑274 (MQ=255)
                                                                                                          aCGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTc                                                                                                                                                                <  1:47123/275‑1 (MQ=255)
                                                                                                          aCGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTc                                                                                                                                                                <  1:100292/275‑1 (MQ=255)
                                                                                                           cGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCa                                                                                                                                                               >  2:204997/1‑275 (MQ=255)
                                                                                                           cGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCa                                                                                                                                                               >  2:80282/1‑275 (MQ=255)
                                                                                                            gCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCATCTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAg                                                                                                                                                              >  2:166403/1‑275 (MQ=255)
                                                                                                                tgtACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAACGCCGCAGAATGTTGAGCGCCCACTGAACCAgggg                                                                                                                                                           >  1:143959/1‑274 (MQ=255)
                                                                                                                 ggaCGAATGGCGGTTGGCACCGGCGATCGTCGGGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGc                                                                                                                                                          <  1:49662/272‑1 (MQ=255)
                                                                                                                  tACGAATGGCGCTTGGGACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCg                                                                                                                                                        <  1:80281/275‑1 (MQ=255)
                                                                                                                      aagggggcTTGGGACCGGCGATCGTCGAGGGCTGAAGACCTGGCTGGCGCGTCTGCAGATGGAAGCGAAAGAGAAAGATGAAGGGCGTGACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGgaga                                                                                                                                                    <  2:52235/269‑1 (MQ=255)
                                                                                                                          gcgcTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCATCTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGgc                                                                                                                                                >  2:73151/1‑275 (MQ=255)
                                                                                                                          gcgcTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGgc                                                                                                                                                >  2:96647/1‑275 (MQ=255)
                                                                                                                                   aCCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTgcg                                                                                                                                       <  1:57355/275‑1 (MQ=255)
                                                                                                                                          aTCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGGATGAGCTGAAGATTGATAAAGCGCCGCAGAATTTTGAAAGCGACACGGCACAGGGGCCGGGGATGGCGCGCGGGCGCGGGtg                                                                                                                                 >  1:218135/1‑273 (MQ=255)
                                                                                                                                                     cggAATACCTGGCTGGCGGGTCTGCCTATGGAAGCGGAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGa                                                                                                                      <  1:107626/272‑1 (MQ=255)
                                                                                                                                                     cTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCATCTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGa                                                                                                                      <  1:73150/274‑1 (MQ=255)
                                                                                                                                                       gAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAAt                                                                                                                    <  1:204996/274‑1 (MQ=255)
                                                                                                                                                       gAATACCTGCCTGGGGCGTCTGCCTATGGAAGCGAAAGAGAAAGAGGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAAt                                                                                                                    <  1:91917/274‑1 (MQ=255)
                                                                                                                                                       gAATACCGGGCTGGGGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAAt                                                                                                                    <  1:15538/274‑1 (MQ=255)
                                                                                                                                                       gAATACCGGGCTGGCGCGTCTGGCTATGGAAGCGAAAGAGAAAGAGGAATGGCGGTACTGGAAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAAt                                                                                                                    <  1:92713/274‑1 (MQ=255)
                                                                                                                                                         aTACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAAtct                                                                                                                  <  1:77422/274‑1 (MQ=255)
                                                                                                                                                                gctggcGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGataata                                                                                                          >  2:149444/1‑275 (MQ=255)
                                                                                                                                                                   ggggcgGCTGCCTATGGAAGCGAAAGAGAAAGATGAAGGGGGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCGGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCGACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATAcc                                                                                                        <  1:233200/271‑1 (MQ=255)
                                                                                                                                                                     cgcgTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCATCTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgcg                                                                                                     <  1:166402/275‑1 (MQ=255)
                                                                                                                                                                         tCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGCGGTTTCTACCCGATGGTTGCAGCACAACGACTCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTAACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTTGTGTAGTGGAATCGCAATAATAACGCGCGt                                                                                                  >  2:81091/1‑274 (MQ=255)
                                                                                                                                                                              cgaTGGAAGCCAAAGAGAAAGATGAATGGCGTTACTTGCAGGCGGATTTATTGCTGGAACGGGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAATTCAGGCAACAGCGTGGTTTCGACCCGATGGTTGCAGGACAACGCAGCGGCGAAGAGTATGGGCTGAAGATTGAGAAAGGGCGGCAGAATGTTGAGAGCGCCCTGACTCAGGGGCCGGAGAGGGCGCGCGTGCGCGAGTTGATGTACTGGAAGCTCGATAAGACCGCGCGTAGCGAg                                                                                            <  1:191685/273‑1 (MQ=255)
                                                                                                                                                                               gagggAAGCGAAAGAGAAAGAGGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAg                                                                                            <  1:81090/271‑1 (MQ=255)
                                                                                                                                                                                    aaGCGAAAGAGAAAGAGTGATGGCGTTACGGGCAGGCGGATTTATTGCTGGAACGCGGACGTTAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGAGAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGc                                                                                       <  2:61568/274‑1 (MQ=255)
                                                                                                                                                                                           agagaaagaTGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGGGTTGATGTACTGGAATCTCGATCATACCGCGCGTAGCGAGTGGGCCCATCTg                                                                                >  2:207845/1‑274 (MQ=255)
                                                                                                                                                                                               aaggaTGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTggg                                                                                        <  1:207844/259‑1 (MQ=255)
                                                                                                                                                                                                   aTGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGc                                                                       <  1:96646/275‑1 (MQ=255)
                                                                                                                                                                                                        tGGCGTTACTGGATGGCGGATTTATTGCTGGAACGCGTACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAAGTGATGCAACAGCGTGGTTTGTACCCGATGGTTGCAGCACGACGCATCGGCGAAGAGTATGAGCTGGAGATTGAGAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTc                                                                  <  2:45100/275‑1 (MQ=255)
                                                                                                                                                                                                             ttACTGGCAGGCTGATTTATTGCTGGAACGCGGACGTGAAGCTGGAGCAAAAGAGAGTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTTGTGAAGAGCAAGTCaaaaa                                                             <  1:68731/275‑1 (MQ=255)
                                                                                                                                                                                                                    aacgCGGATTTATGTCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTAGTTTCTACCCTATGGTTGCAGCACAACGCAGCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACagag                                                        <  2:50803/270‑1 (MQ=255)
                                                                                                                                                                                                                     aGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAg                                                                                                                                                                           >  2:42876/1‑157 (MQ=255)
                                                                                                                                                                                                                     aGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGGGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGccg                                                     >  2:152632/1‑273 (MQ=255)
                                                                                                                                                                                                                         ggATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGcc                                                                                                                                                                       <  1:42876/157‑1 (MQ=255)
                                                                                                                                                                                                                                         cgcgGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGa                                                                                                                      <  1:220013/190‑1 (MQ=255)
                                                                                                                                                                                                                                                                agagATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAgcg                                                                                                                                                                                            >  1:65601/1‑97 (MQ=255)
                                                                                                                                                                                                                                                                  agaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGGATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCtt        <  1:149443/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                    aTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGcgccgc                                                                                                                                                                                        <  2:65602/97‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tGCAGCAACGCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGtt  <  1:223602/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                           
TGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTT  >  NC_000913/4631575‑4632113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: