Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA98.2% 156.4 / NA 54P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (28/25);  minor base T (0/1);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

ACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC  >  NC_000913/4184414‑4184678
                                                                                                                                |                                                                                                                                        
atggcGTATCCGGTCTTGTGACTAGGTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                            <  1:403516/139‑1 (MQ=255)
aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCGGATCGAAAAGATGCCGTACGAGGAAAACGGTACGCCGGTAGACATCATACTGAGGCAGCTGAGCGGACCGt                                                                                                                            >  1:572207/1‑143 (MQ=255)
aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                            >  2:515226/1‑143 (MQ=255)
aCGCCGTATCCAGCCTGGTGACAAGAGGGCAGGTCGTCGCGGTAACACGGGTGTAATTTCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                            <  2:668/143‑1 (MQ=255)
 cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtc                                                                                                                           >  2:59242/1‑143 (MQ=255)
  gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtct                                                                                                                          >  2:339109/1‑143 (MQ=255)
    cGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGGGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGGAGACATCGTACTGAACCAGCTGGGCGTAGCGTCACg                                                                                                                        >  1:317012/1‑143 (MQ=255)
       aTCCAGCCTGCTGACACGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGCTGAAAACGGTACGCCCGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                     <  1:858354/143‑1 (MQ=255)
         cccgCCGGGTGAGCAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa                                                                                                                   <  1:274225/140‑1 (MQ=255)
         ccAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTGCTAAGATCCACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa                                                                                                                   <  2:595115/143‑1 (MQ=255)
          cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                  >  1:273039/1‑143 (MQ=255)
           acccTGGTCACAAGACGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAc                                                                                                                 <  2:872204/141‑1 (MQ=255)
             ccTGGTGACAATATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAt                                                                                                               <  2:521148/143‑1 (MQ=255)
                 gggacatctgtgCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                           <  1:325407/132‑1 (MQ=255)
                  tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                          >  2:719925/1‑143 (MQ=255)
                   gACAAGATGGCAGGTCGTCACGGTAACACGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTAGGCGTGCGGTCCCGTACGAACCTCGGTCa                                                                                                         >  1:56721/1‑143 (MQ=255)
                      aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGACGATATGCCTTACGATGAAAACGGTACGCAGGTAGACAGCGTACTGCACCAGCTGGGCGTACCGTCTCGTATGAACacagctccgat                                                                                                      >  1:298969/1‑133 (MQ=255)
                      aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGAt                                                                                                      >  1:165228/1‑143 (MQ=255)
                         agggCAGGGCGTCACGGTAACAAGGGTGTAATTGCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                   <  1:888043/141‑1 (MQ=255)
                                      aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAAAACGGTCAGATCCTAGAAacacacaag                                                                                      >  2:184701/1‑140 (MQ=255)
                                          tAACAAGGGTGTAATGTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTa                                                                                  <  1:504152/143‑1 (MQ=255)
                                                gggTGTCATTGCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGCTATACATCGGACTGACCCACCTGGGCGTACCGTCTCGTATGAACACCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTg                                                                            <  1:734623/143‑1 (MQ=255)
                                                 ggTGTAAGTTCTAAGATCAACCCGATCGAAGATATGCCTTACGCTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                           <  2:595690/143‑1 (MQ=255)
                                                 ggTGGAATTGCTAAGATCGACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                           <  2:714324/143‑1 (MQ=255)
                                                  gtgtAATTTCTAATACCAACCCGAGCGAAACTATGCCTTACGATGAAAACGGTACGCCGCTACCCACCTCACTTCACCTGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCg                                                                          <  1:772286/143‑1 (MQ=255)
                                                    gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaa                                                                        >  2:155938/1‑143 (MQ=255)
                                                     tAATTTCTAAGATCAACCCGATCGAAGATATGCCTAATGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                       >  2:655174/1‑143 (MQ=255)
                                                     tAATTTCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                       <  1:707964/143‑1 (MQ=255)
                                                      aaTTTGTAAGAGCAACCCGAGCGAAGATATGCCTGACGATGAAAACGGTGCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                      <  2:271044/143‑1 (MQ=255)
                                                       aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACAAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATGCTCGAAACCCACCTGGGTATCGCTCCGCAAgg                                                                     >  2:107899/1‑143 (MQ=255)
                                                        tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGt                                                                    >  1:274001/1‑143 (MQ=255)
                                                          tCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATAGCTGCGAAAGGTAt                                                                  >  2:825495/1‑143 (MQ=255)
                                                               gATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCg                                                             <  2:226603/143‑1 (MQ=255)
                                                                  cagcccGATCGAAGATATGCCTTACGATGAAATCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACa                                                          <  1:205553/140‑1 (MQ=255)
                                                                   aaCGCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                         <  2:364053/143‑1 (MQ=255)
                                                                        gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACTTGGGTATGGCTGCGACAGGTATCGGCGACAAGCTCa                                                    >  2:864872/1‑143 (MQ=255)
                                                                        gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAACCCCACGAAACCCGCCTGGGTCCCGCGCCCCAAGCCATCGGCGACAAGATCa                                                    >  2:594064/1‑143 (MQ=255)
                                                                            gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCGACAAGATCAACGc                                                >  1:166937/1‑143 (MQ=255)
                                                                                    gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAACGGTATCGGCGACAAGCTCAACGCCATGCTGa                                        >  2:241467/1‑143 (MQ=255)
                                                                                           gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                 >  1:549815/1‑143 (MQ=255)
                                                                                           gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAGCAACGCCATGCTGAAAcagca                                 >  2:94423/1‑143 (MQ=255)
                                                                                           gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGAGCGTACCGTCTCGTGTGAACATCGGTCAGATCCCCGAAACCAACCTGGCTATGACTGCGACGTGTATCGGCGACAAGCTCAACGCCATGCTGAAAcagca                                 >  1:458902/1‑143 (MQ=255)
                                                                                             tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                               >  2:359407/1‑143 (MQ=255)
                                                                                               aaacCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                             <  2:365354/143‑1 (MQ=255)
                                                                                               aaaaCGGCACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                             <  2:365012/143‑1 (MQ=255)
                                                                                                 aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGGaaga                           >  2:889488/1‑143 (MQ=255)
                                                                                                        cGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCGTCGAAACCCACATGAGTACTACAGCGTAACGTATCGGCGACCCGCTCCACGCCGTGCTGGAACCGCAGCAAGAAGTcgcg                    >  1:826245/1‑143 (MQ=255)
                                                                                                            ggTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACCAGCTCAACGCCCTGCTGAAACAGCAGCAAGAAGTCGCGAAAc                >  2:462302/1‑143 (MQ=255)
                                                                                                                 ccATAGTACTGAACCAGCTGAGCGTACCGTCTCGTATGAACACCGGTCAGATCCTCGAAACCCACGTGAGTCGGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCCTGCCGCCACAGCAGCCAGAAGCACCGAACCTgcgc           >  1:184405/2‑143 (MQ=255)
                                                                                                                 aCATCGTACTTAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAATTTATCGGCGACAAGCTCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc           <  2:358571/143‑1 (MQ=255)
                                                                                                                    tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa        <  2:3644/143‑1 (MQ=255)
                                                                                                                       caCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTc     <  2:762912/142‑1 (MQ=255)
                                                                                                                        aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa    <  2:274001/143‑1 (MQ=255)
                                                                                                                          tGAACCAGCTGGGCGTCGCGTCTCGTATGAACATCGGTCAGACCCTCGAAACCCACCTGGGTATGGCGGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc  <  1:859582/143‑1 (MQ=255)
                                                                                                                                |                                                                                                                                        
ACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC  >  NC_000913/4184414‑4184678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑