Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,269,986 A→C 7.7% intergenic (+386/+228) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9860AC 7.7% 131.9 / 2.1 51intergenic (+386/+228)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base A (24/20);  new base C (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

AACCGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAG  >  NC_000913/3269708‑3270233
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
aaCCGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                                                                                                                                                                                                                                <  2:643546/288‑1 (MQ=255)
 aCCGCAAAAATGCCTGATGCGCTACGCTTTTCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACt                                                                                                                                                                                                                                              <  2:178994/289‑1 (MQ=255)
   cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGTGTCGTGGACCGTCGTTCATCTTGCGGCCCCTCAAAAAGTAAAAACCTGACGAAGAGGGTTATTACGTTACAAAAGTGAAACTGa                                                                                                                                                                                                                                            >  2:750994/1‑289 (MQ=37)
               tgttGCGCTACGCTTATCACGCCTACGCACTCTCTGCAATATATTGAATTTGCCTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAGCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGc                                                                                                                                                                                                                                <  1:750994/286‑1 (MQ=255)
                gATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGct                                                                                                                                                                                                                               <  1:273747/289‑1 (MQ=255)
                  tgtttataatagacagggCCTACGCCTACTTTGCAATATTTTGAATTTGCGTGCTTTTGTAGGCAGGAGAAGGCGTTCACGCCGCCACCGGCATAAATATAGCCAACTCAACAATCTGAAACCCGCCGGAACGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGct                                                                                                                                                                                                                               <  2:99004/271‑1 (MQ=17)
                         cGCTTATCAGGCCTACGCCATCTATGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTATTTTgg                                                                                                                                                                                                                      <  1:631323/289‑1 (MQ=255)
                            ttATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta                                                                                                                                                                                                                   <  1:76999/289‑1 (MQ=255)
                            aggtgacggcTACGCGATCTCTGCAACATATTGAATTTGCTGGCTGTTGAAGGCAGGGTAAGGCGTTCACGCGGCATCTGGCATGAATAAAGCGAACTCAACATGCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta                                                                                                                                                                                                                   <  2:338176/280‑1 (MQ=18)
                              aTCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                                                                                                                                 <  1:253639/289‑1 (MQ=255)
                                        cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACCGGTGAAACTGACCGATAAGCCGCCTTCTTTTGGGTTTAgcgtattgg                                                                                                                                                                                                        >  2:500371/1‑283 (MQ=255)
                                         gcaaTCCCTGCAATAAATTGAATTTGCGTGCTTTTGTCGGCAGCATAAGGCGTCCACGCGGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAACCCCGCCGGAACGTTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGCGGACAGTCATTCAACTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAc                                                                                                                                                                                                      <  2:22586/286‑1 (MQ=21)
                                             tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGCTAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGtc                                                                                                                                                                                                  >  1:173310/1‑289 (MQ=255)
                                             tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtctgttttctttacacg                                                                                                                                                                                                    >  1:99004/1‑271 (MQ=255)
                                                   cAATATATTGAATTTGCGTGCTTTTGTAAGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattca                                                                                                                                                                                             >  1:499502/1‑288 (MQ=255)
                                                         atTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCt                                                                                                                                                                                      >  1:405451/1‑289 (MQ=255)
                                                         atTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGCAACTGAACGATAAGCCGCTTTCTTTTGTGGATAGTGTCTCGTACAGTCCTTCAACttc                                                                                                                                                                                        >  2:424084/1‑286 (MQ=21)
                                                                  gCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTTTCGTGGACAGTCATTCATCTTTCTGCCCCTCCa                                                                                                                                                                             >  1:586190/1‑289 (MQ=255)
                                                                       cTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCAGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTGCTGCCCATCCAAAAg                                                                                                                                                                         >  1:351219/1‑288 (MQ=255)
                                                                                  aGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGCTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                                                                             >  1:579822/1‑289 (MQ=255)
                                                                                            ggtaaCGCCGAATCCGGCATGAATAAAGCGAACTCCACAATCGGAAACCCTCCCGAACGGTTTATTACGTACAACAGGTAAAACTGACCGATAAGCCGCTTTCTTTTTGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                                                                                                                                                   <  2:29021/285‑1 (MQ=255)
                                                                                              tCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                                                                                                                                 >  1:10207/1‑225 (MQ=255)
                                                                                              tCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                                                                                                                                 <  2:10207/225‑1 (MQ=255)
                                                                                              tCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                                                                                 >  2:240155/1‑289 (MQ=255)
                                                                                                  gccgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                                             <  1:240155/289‑1 (MQ=255)
                                                                                                                           aCTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTCAACCAGGTGAAACTGACCGATaa                                                                                                                    >  1:470975/1‑289 (MQ=255)
                                                                                                                                   aTCTGAAAACCGACGGATCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCCTTTTGGTTTAGTGTCCTGGACAGGCATTCATCTTGCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcttt                                                                                                            <  2:35547/289‑1 (MQ=14)
                                                                                                                                   aTCGGAAACCCGCAGGAACTGTTGATTACGTACAATAGTGAAAACTTACCGATAATCTGTTTTCTTTTGGGGATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcttt                                                                                                            <  2:499502/289‑1 (MQ=14)
                                                                                                                                      tGAAACCCGCCGGAACGGATTATTACGTACATAAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttctt                                                                                                         <  1:500371/289‑1 (MQ=255)
                                                                                                                                       gAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGTCAGTCATCCTGCTGCCCCTCCAAAAGCAATAACCCGCCGAAGCGGGTTTTTACGCAAACCCGCTGAAACTGACCGATAAGCCGctttcttt                                                                                                        >  1:502407/1‑289 (MQ=255)
                                                                                                                                       gAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                                        >  1:35547/1‑289 (MQ=255)
                                                                                                                                         gaCCCGGCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCACTCCAAAAGTAAAACCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                       <  1:256465/287‑1 (MQ=255)
                                                                                                                                              gccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTGTCTTTTGGGTATAGTGTCGTGGTCAGtcattcat                                                                                                                                                                                            >  1:687075/1‑198 (MQ=255)
                                                                                                                                               acgcaaCGGATTATTACGAGACACAGGTGAAACTTTACGATAAGCCGGCGTCAATTGGGTACAGTGTGGTTGAAACTCATTCAACCTTCAGACACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGTCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                <  2:92614/285‑1 (MQ=11)
                                                                                                                                                           aatacggtacccaaGGTAAAACGGACCGTTATGCTGCTTGCATTTGGGATTATTGCCGCGGAAAGCAGTTCATATTACTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTATTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAc                                                                                     <  2:405451/276‑1 (MQ=14)
                                                                                                                                                                   aCATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGTTTCTGTTGGGTATAGTGTCGTGTACAGGCAACCAACTTTCCGCCACTCCAACAGCAAAACCCGGCCGAAGCGCGTTCTTACGTAAACCCTGTGACACTGCCAGATAAGCGGTCTTCTTTTGCGTACAGtgtcttagctatgcagt                                                                              >  2:532843/1‑275 (MQ=14)
                                                                                                                                                                     aTCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATTGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACTTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGACAGccactca                                                                            >  1:413323/1‑288 (MQ=255)
                                                                                                                                                                     aTCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAATCCGAGTTCTGTTGGGTATAGCTGCGTGGACAGtcattcat                                                                           >  1:275399/1‑289 (MQ=255)
                                                                                                                                                                       cAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGGAAAAACCCGCCGAAGCGGTGTTTTACGTAAACCAGGTGAAACTCACCGATAAGCCGCTTTCTATCGGGGATAGTTTCGCGTCCAGTCATTCAAATTTCTACACGCCCAAACACAAAACCCCGCCTAAGCGGTTTTTTACGTACACACGTGGACACTGCCCAGAAAGCCGAGTTCTTTAGGTCATAGctgctaggacctccatgaatca                                                                         >  2:741788/1‑268 (MQ=2)
                                                                                                                                                                         ggTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta                                                                                                                                                                                                                   <  2:155678/149‑1 (MQ=37)
                                                                                                                                                                                   aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTg                                                                                                                                                                                                         >  1:155678/1‑149 (MQ=37)
                                                                                                                                                                                   aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGTACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAACCCGCGTTCTTTGTGGTATACCGTTGTGGACAGTCATCCACCCTTCTGCCCCTc                                                              >  1:408434/1‑288 (MQ=14)
                                                                                                                                                                                                    ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGa                                                                                                                                                                                                       <  2:50566/134‑1 (MQ=37)
                                                                                                                                                                                                              aGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattca                                                                                                                                                                                             >  1:50566/1‑134 (MQ=37)
                                                                                                                                                                                                                tgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGGATAGTGTCGTGGACAGTCATTCATCCTTCCGCCCCTCCAAACGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTCAACTTGACCGTTCACCCGCTTTCTGTTGGGCATACCATCTAGGGCACTCCTATAACCTTCTCCCGCTCCCAACGCAAAAAccgcaccaagccgggttg                                >  2:763211/1‑273 (MQ=11)
                                                                                                                                                                                                                     tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGa                                                                                                                                                                                                       >  1:538407/1‑117 (MQ=39)
                                                                                                                                                                                                                     tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGa                                                                                                                                                                                                       <  2:538407/117‑1 (MQ=39)
                                                                                                                                                                                                                                       tCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAACGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGCAACTGACAGATAAGCCGCGTTCTTTTGGGTATCGCGTCGTGAACAGTCATTCATCTTTCTGCACCTCCACCAGCAAAAACCCGCCCAAGCGGGTTTTTACGTAGATCaatcaaaactga          >  1:346966/1‑277 (MQ=17)
                                                                                                                                                                                                                                           tgccactaaaagtgagttaccgccaAAGTGTGTTTTTAAGTAAACACGGTGAAATGTAGCTATGAGGCGTTTTTTTGTGTGTATAGGTTTGCCGACAGTCAATCATCTTTCTTCGCCTACAAAAGCAAAGACGCGTCGAAGCGGTTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCATGGACAGTCATTCATCTTTCTGCCCCTCCAAAGGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGAt     <  2:413323/270‑1 (MQ=2)
                                                                                                                                                                                                                                              ccacaataaataaatcacgccggacagggTTATTACATAGAACAGGATAAACTGACCGAGATGCAGCTTTCTTTTTCGTATGTTGACGATGACTGTGCTTCAACTATGGGCCCCTCCACAATATTAATCCCGCCGGTGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGATATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAg  <  2:586190/263‑1 (MQ=2)
                                                                                                                                                                                                                                              cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTTTTTGGTACAGTGTTTCGGATAGGCCTTCGTCTGTCCAGCGCCACAAACACACTACCCCGCCCACGCTGGTGTTTACGCAAATAAGGTCAAATTGGCCCATaa   >  2:565959/1‑288 (MQ=11)
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
AACCGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAG  >  NC_000913/3269708‑3270233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: