breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsEvo04sdhCadhBEvo03EP_S22_L001_R1_001.good.fq769,465209,264,854100.0%272.0 bases289 bases99.8%
errorsEvo04sdhCadhBEvo03EP_S22_L001_R2_001.good.fq769,465209,328,254100.0%272.0 bases289 bases98.2%
total1,538,930418,593,108100.0%272.0 bases289 bases99.0%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65393.13.7100.0%Escherichia coli str. K-12 sbstr. MG1655, complete genome. Live strain from Systems Biology Research Group
total4,641,653100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000002624
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000163
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.021

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.88591

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoffOFF
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.025
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.2.8
R3.3.1

Execution Times

stepstartendelapsed
Read and reference sequence file input01:59:04 18 Mar 201801:59:28 18 Mar 201824 seconds
Read alignment to reference genome01:59:28 18 Mar 201802:01:24 18 Mar 20181 minute 56 seconds
Preprocessing alignments for candidate junction identification02:01:24 18 Mar 201802:01:52 18 Mar 201828 seconds
Preliminary analysis of coverage distribution02:01:52 18 Mar 201802:02:50 18 Mar 201858 seconds
Identifying junction candidates02:02:50 18 Mar 201802:02:53 18 Mar 20183 seconds
Re-alignment to junction candidates02:02:53 18 Mar 201802:03:14 18 Mar 201821 seconds
Resolving best read alignments02:03:14 18 Mar 201802:04:02 18 Mar 201848 seconds
Creating BAM files02:04:02 18 Mar 201802:04:48 18 Mar 201846 seconds
Tabulating error counts02:04:48 18 Mar 201802:05:22 18 Mar 201834 seconds
Re-calibrating base error rates02:05:22 18 Mar 201802:05:23 18 Mar 20181 second
Examining read alignment evidence02:05:23 18 Mar 201802:36:04 18 Mar 201830 minutes 41 seconds
Polymorphism statistics02:36:04 18 Mar 201802:36:04 18 Mar 20180 seconds
Output02:36:04 18 Mar 201802:36:20 18 Mar 201816 seconds
Total 37 minutes 16 seconds