Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 759,508 (TGAACGTGCTGG)2→1 coding (803‑814/2802 nt) sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 759508 759519 12 25 [0] [0] 25 sucA 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 7595070 (0.000)24 (0.660) 18/260 1.1 100% coding (802/2802 nt) sucA 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
?NC_000913 759520 = 0 (0.000)coding (815/2802 nt) sucA 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

TTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/759366‑759507
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTT  >  NC_000913/759520‑759659
                                                                                                                                                                                                                                                                                          
TTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTGCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGG                                                                                                                                             >  1:473774/1‑143
                         TGGAAGGCGGTCACGCGTCAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGAC                                                                                                                    <  1:246940/143‑1
                               GCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGGCACGCTGGCAACAGCGGCACGCGCGTAGTGGTTGTGGGGATGGCGCGCCGTGGGCGTCGGAACGGGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTC                                                                                                              >  2:528390/1‑143
                               GCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCCCCCGCGCAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAGAAAACCGCACGACGTGTTCGACGAGTTC                                                                                                              >  2:35493/1‑143
                               GCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGCACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTC                                                                                                              >  1:549145/1‑143
                                   TGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCG                                                                                                          <  1:27689/143‑1
                                       GCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAA                                                                                                      >  1:208913/1‑143
                                              gccgcATGCTTAAAGAGAGGATCCGCCACTCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAAAATACCGCAAGACTGGTTCGACGAGTTCGCCGGTAAACATAAA                                                                                               <  1:494144/138‑1
                                                CCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAACCCTAAAGA                                                                                             >  1:253066/1‑143
                                                 CGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAA                                                                                            <  1:605379/143‑1
                                                         AAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAAAAAACCGCAAGGCTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGG                                                                                    >  1:318612/1‑143
                                                                 GATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGGGCTGGGGAACAAACCGCAAGACTTGTTCGACGAGTGCGCCGCTAAACATAAAAGACACCCCGGCACGGGTG                                                                            >  2:586265/1‑143
                                                                          ctctaGCAACAGCCGGACCCGCGAATTGGGTCTCGGCATGGCGCACCGTGCTCGTCCGAACGTGCTGTGTAAAAAACCGCAGGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGACCACCTCGGCACGGGTGACGTGAAAT                                                                   <  2:622985/138‑1
                                                                                       GGCACCCGCGAAGTGGTTCTCGGGATGGCGCAACGTGGTCGCCTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTT                                                      <  1:541571/143‑1
                                                                                              ggGAAGTGGTGCGCGGGAGGGCGCACCGTGGGGGTCTGAAGGGGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACGTCGGAAGGGGGGACGTGAAATACCACATGGGCTTCTCGTCT                                               <  2:247258/141‑1
                                                                                              GCGAAGTGGTTCTCGGGATGGCGCAACGTGGTCGTCTGAACGGGCGGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCT                                               <  1:85566/143‑1
                                                                                                GAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCGCATGGGCTTCTCGTCTGA                                             <  1:40917/143‑1
                                                                                                  agcggtcgtccGGATGGCGCACCGTTGTCCTCTGAACGTGCTGGATAAAACCCCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCTCGGGTGACGTGAAAGACCACATGGGCTTCTCGTCTGACT                                           <  2:203217/132‑1
                                                                                                        TCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAAAAAACCGCCAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCTGCACGGGTGACGTGAACTACGCCATTGGCTTCTCGTCTGACTTCCtct                                     >  1:421074/1‑140
                                                                                                        TCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGA                                     >  1:526804/1‑143
                                                                                                                     CACCGTGGTCGGCTGAGCGGGCTGGGTAAAAATCCGCAAGACGTGTTCGACGAGGTCGCCGGTAAACATAAAGAGCACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCT                        <  2:438339/143‑1
                                                                                                                     CACCGTGGGCGGCTGAGCGTGCTGGGTGAAAGTCCGCACGACGCGTACGACGCGGTCGCCGGTAAACATAAAGAGCACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCT                        <  2:284681/143‑1
                                                                                                                            GTCGTCTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCAC                 <  1:74138/143‑1
                                                                                                                             TCGTCTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAGCATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACC                >  1:413033/1‑143
                                                                                                                                           TGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACGTGGCGCTGGCGTTT  >  1:630244/1‑143
                                                                                                                                                                                                                                                                                          
TTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/759366‑759507
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTT  >  NC_000913/759520‑759659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.