Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 83.1 / NA 30I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/15);  total (15/15)

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT  >  NC_000913/2132647‑2132922
                                                                                                                                            |                                                                                                                                       
ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc                                                                                                                                       <  2:593008/143‑1 (MQ=255)
     tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATATGTCTATGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATAGATATGCAGGCTCTGCgc                                                                                                                                  >  2:365263/1‑142 (MQ=255)
             ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                          >  1:500415/1‑143 (MQ=255)
             ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGGCGCGtctt                                                                                                                          >  1:80698/1‑143 (MQ=255)
                    ggCTGATATTTTTGGCTGGCGCGGTCGAATTTGTTTTGGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt                                                                                                                   <  2:435090/142‑1 (MQ=255)
                       tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                >  2:507947/1‑143 (MQ=255)
                          tatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGCAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGCCTCTTCTACTCTCCACgg                                                                                                             >  2:157141/1‑143 (MQ=255)
                           atTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAGAGTGTAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGt                                                                                                            <  2:678752/143‑1 (MQ=255)
                               tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCCCGGTTCGc                                                                                                        >  2:460964/1‑143 (MQ=255)
                                ttGCTGGCGCGGTCGAATTTGGCTTTGTGCTGGCGGTAAAAGTGGAAGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                       <  2:633288/143‑1 (MQ=255)
                                          ggccgtatctctCTGTGTGCTGGCGGTAAAGGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                             <  2:268717/132‑1 (MQ=255)
                                                    gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATGGATATGCAGGCTCTGCGTCGCCTCTTCGACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGcg                                                                                   >  2:336644/1‑142 (MQ=255)
                                                              ttGCGTTAACGGTGGCAGTACCCTGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATACGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                         <  2:266756/143‑1 (MQ=255)
                                                               tGCGGTACACGTGGAAGTACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                        <  1:166383/143‑1 (MQ=255)
                                                               tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCAACCACCATCCGCACGCAGACGtac                                                                        >  2:379520/1‑141 (MQ=255)
                                                                   gTAAAAGTGGAAGTACCCGGAGAATTTTGTCGGTGACGAGGTGATCTGCATTTCACCGCGATGGATATGCAGGCTCTGCGTCGCAACTTCTACTTTCCACGGGGCGCCGTACTCCACCACCCTCCGCAGGACGagggcgccgg                                                                    >  2:152855/1‑134 (MQ=255)
                                                                    tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCGACGGTTCGCCGTACTCCACCACCATCCGCAGTGAGTTGTCGTAAtg                                                                   >  1:196855/1‑142 (MQ=255)
                                                                        aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                               <  1:600817/143‑1 (MQ=255)
                                                                         gTGGGAGTGCGCGGAGAAGTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAATCCtgtg                                                              <  2:156268/143‑1 (MQ=255)
                                                                               gTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTAGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTACCGCtt                                                        >  2:518342/1‑143 (MQ=255)
                                                                                  ccctgagcgttttttGTGTGACGGTGTGATCTGCTTTTCCCCGTGCTTGATCTGCCCGCTCTTTGTTGCCTCGTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCTGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                     <  2:419044/134‑1 (MQ=255)
                                                                                     ggAGAAGGTATTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTGTTATACTTTCCACGGTTCGCCGTACTCAACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                  <  1:148700/143‑1 (MQ=255)
                                                                                      gagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGCTc                                                 >  2:299238/1‑143 (MQ=255)
                                                                                                  ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa                                     <  1:560369/143‑1 (MQ=255)
                                                                                                        gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACCAACtctc                               >  1:10936/1‑143 (MQ=255)
                                                                                                        gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActc                               <  1:507947/143‑1 (MQ=255)
                                                                                                               tCTGCATTGCACCCTGCGTGGTATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCGCGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGCAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                        <  2:515560/143‑1 (MQ=255)
                                                                                                                cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTATCAGTTCGGTATCGAACAAACACTCTGTGAGAc                       >  1:71538/1‑143 (MQ=255)
                                                                                                                                ttgacATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa       <  1:530494/143‑1 (MQ=255)
                                                                                                                                     atGCAGGCTCTGCGTCACCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAACCGCCATGCCCAGGTAAAGACCt  >  1:122187/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                       
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT  >  NC_000913/2132647‑2132922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: