Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	83.1 / NA	30	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/15);  total (15/15)

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT  >  NC_000913/2132647‑2132922                                                                                                                                             |                                                                                                                                        ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc                                                                                                                                       <  2:593008/143‑1 (MQ=255)      tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATATGTCTATGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATAGATATGCAGGCTCTGCgc                                                                                                                                  >  2:365263/1‑142 (MQ=255)              ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                          >  1:500415/1‑143 (MQ=255)              ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGGCGCGtctt                                                                                                                          >  1:80698/1‑143 (MQ=255)                     ggCTGATATTTTTGGCTGGCGCGGTCGAATTTGTTTTGGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt                                                                                                                   <  2:435090/142‑1 (MQ=255)                        tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                >  2:507947/1‑143 (MQ=255)                           tatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGCAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGCCTCTTCTACTCTCCACgg                                                                                                             >  2:157141/1‑143 (MQ=255)                            atTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAGAGTGTAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGt                                                                                                            <  2:678752/143‑1 (MQ=255)                                tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCCCGGTTCGc                                                                                                        >  2:460964/1‑143 (MQ=255)                                 ttGCTGGCGCGGTCGAATTTGGCTTTGTGCTGGCGGTAAAAGTGGAAGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                       <  2:633288/143‑1 (MQ=255)                                           ggccgtatctctCTGTGTGCTGGCGGTAAAGGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                             <  2:268717/132‑1 (MQ=255)                                                     gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATGGATATGCAGGCTCTGCGTCGCCTCTTCGACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGcg                                                                                   >  2:336644/1‑142 (MQ=255)                                                               ttGCGTTAACGGTGGCAGTACCCTGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATACGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                         <  2:266756/143‑1 (MQ=255)                                                                tGCGGTACACGTGGAAGTACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                        <  1:166383/143‑1 (MQ=255)                                                                tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCAACCACCATCCGCACGCAGACGtac                                                                        >  2:379520/1‑141 (MQ=255)                                                                    gTAAAAGTGGAAGTACCCGGAGAATTTTGTCGGTGACGAGGTGATCTGCATTTCACCGCGATGGATATGCAGGCTCTGCGTCGCAACTTCTACTTTCCACGGGGCGCCGTACTCCACCACCCTCCGCAGGACGagggcgccgg                                                                    >  2:152855/1‑134 (MQ=255)                                                                     tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCGACGGTTCGCCGTACTCCACCACCATCCGCAGTGAGTTGTCGTAAtg                                                                   >  1:196855/1‑142 (MQ=255)                                                                         aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                               <  1:600817/143‑1 (MQ=255)                                                                          gTGGGAGTGCGCGGAGAAGTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAATCCtgtg                                                              <  2:156268/143‑1 (MQ=255)                                                                                gTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTAGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTACCGCtt                                                        >  2:518342/1‑143 (MQ=255)                                                                                   ccctgagcgttttttGTGTGACGGTGTGATCTGCTTTTCCCCGTGCTTGATCTGCCCGCTCTTTGTTGCCTCGTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCTGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                     <  2:419044/134‑1 (MQ=255)                                                                                      ggAGAAGGTATTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTGTTATACTTTCCACGGTTCGCCGTACTCAACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                  <  1:148700/143‑1 (MQ=255)                                                                                       gagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGCTc                                                 >  2:299238/1‑143 (MQ=255)                                                                                                   ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa                                     <  1:560369/143‑1 (MQ=255)                                                                                                         gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACCAACtctc                               >  1:10936/1‑143 (MQ=255)                                                                                                         gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActc                               <  1:507947/143‑1 (MQ=255)                                                                                                                tCTGCATTGCACCCTGCGTGGTATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCGCGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGCAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                        <  2:515560/143‑1 (MQ=255)                                                                                                                 cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTATCAGTTCGGTATCGAACAAACACTCTGTGAGAc                       >  1:71538/1‑143 (MQ=255)                                                                                                                                 ttgacATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa       <  1:530494/143‑1 (MQ=255)                                                                                                                                      atGCAGGCTCTGCGTCACCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAACCGCCATGCCCAGGTAAAGACCt  >  1:122187/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                        GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT  >  NC_000913/2132647‑2132922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑