Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,001,644	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001644	4001648	5	39 [0]	[0] 38	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001643	0 (0.000)	38 (0.990)	32/284	0.2	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001649 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

CAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTA  >  NC_000913/4001501‑4001773                                                                                                                                               |                                                                                                                                   cAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCat                                                                                                                                    <  2:437146/143‑1 (MQ=255)   gTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTGTTTGACGACGACGCCGGCCTGCGTct                                                                                                                                  >  2:317691‑M1/1‑141 (MQ=255)     aTGGATTGTTCGTGTCGAACCGGACGCCGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctcc                                                                                                                                <  1:437741‑M1/143‑5 (MQ=255)               cTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttct                                                                                                                      >  2:399473‑M1/1‑129 (MQ=255)                ttGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctt                                                                                                                     <  2:282818‑M1/143‑16 (MQ=255)                  gTCGAACCGGACGACGACGAGCGACTGCGCGAACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGTCATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttg                                                                                                                   <  2:251415‑M1/143‑18 (MQ=255)                      aaCCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaaga                                                                                                               >  2:54075‑M1/1‑122 (MQ=255)                      aaCCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCACctccttcttcttctttgaaga                                                                                                               >  2:201433‑M1/1‑122 (MQ=255)                             cgcctccgatcgactGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCCACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaa                                                                                                        <  1:17040‑M1/137‑29 (MQ=255)                              gacgacgaGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGCAGCATCGGCACGTTTCTTTGAAGACGACGACGGACTGCATctacttcttcatctttggagatgcgggag                                                                                                       >  1:129478‑M1/1‑114 (MQ=255)                                  acgcgcgggtgcgcggacaAGCGGGACTTGGCCAGAGCCTGGCAACCCGCCCGGAACGGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatca                                                                                                   <  1:195333‑M1/134‑34 (MQ=255)                                     aGCGACTGCGCGTACAATCCGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgc                                                                                                <  2:595086‑M1/143‑37 (MQ=255)                                       cGACCGCGCGCACACGCTGCACGTGGCCCGAGCCTGGCAACCCGCTCGGAACTGGAAGACATCGAAGCAGCGGCACGTTTCGTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccg                                                                                              <  1:496205‑M1/143‑39 (MQ=255)                                         aCTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggt                                                                                            >  1:437343‑M1/1‑103 (MQ=255)                                              gcgTACAATCTGAACTTGGCCAGAGCCTGGCAACCGGCCCGTATGTGGGAGACATCGAAGCATCGGCACGTTTCTTTGACGACGACGACGGCCTGCATctccttctgcttcgttgaagatgcggaagatcacgcaggtaactc                                                                                       >  2:315139‑M1/1‑98 (MQ=255)                                                     gtcTGGACTGGGCCAGAGCCTGGCAACCCGCCCGGAAGTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagagcacgccggtaactccactgtg                                                                                <  1:538200‑M1/142‑53 (MQ=255)                                                             ttGGCCAGGGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttctgcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttac                                                                        <  2:77837‑M1/143‑61 (MQ=255)                                                             ttGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttac                                                                        <  2:444808‑M1/143‑61 (MQ=255)                                                              tGGCCAGAGCCTGGCAACCCGGCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttacc                                                                       <  1:276496‑M1/143‑62 (MQ=255)                                                                 ccAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatc                                                                    >  2:99387‑M1/1‑79 (MQ=255)                                                                 ccAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccacggtggcatttacaatc                                                                    >  2:320568‑M1/1‑79 (MQ=255)                                                                        cTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgcgatg                                                             >  2:255347‑M1/1‑72 (MQ=255)                                                                          ggCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagctcacgccgctacctccaccgtgccacttaccctccgtgctgtc                                                           >  2:221661‑M1/1‑70 (MQ=255)                                                                               cccgcccgGAACTGGAAGACATCGAAGCAGCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtct                                                      <  2:179085‑M1/143‑79 (MQ=255)                                                                                      ggAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttact                                               <  1:317691‑M1/143‑86 (MQ=255)                                                                                      ggAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttact                                               <  2:583238‑M1/143‑86 (MQ=255)                                                                                          cTGGAAGACATCGAAGCATCGGCACGGTTCGTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgc                                           <  2:517594‑M1/143‑90 (MQ=255)                                                                                            ggAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgt                                         <  2:576399‑M1/143‑92 (MQ=255)                                                                                                gACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagc                                     <  2:322963‑M1/143‑96 (MQ=255)                                                                                                gACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagc                                     <  2:322968‑M1/143‑96 (MQ=255)                                                                                                               gCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgacctgcccgctt                      >  2:117587‑M1/1‑33 (MQ=255)                                                                                                                  cgtaggtgggaagacgacgACGGCCTGCATctccttctacttctttgaagatgcggaagatcacgccggtcaccccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttc                   <  2:251395‑M1/134‑114 (MQ=255)                                                                                                                      tCTTTGAAGACGACGACGGCATGCATctccttcttcttctttgaagatgcagaatatcacgccggtaactccactgtagcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtct               <  2:11346‑M1/143‑118 (MQ=255)                                                                                                                         ttGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttagtctgcgggagggtgaagcgccggcgttgcgtatgga            >  1:615272‑M1/1‑23 (MQ=255)                                                                                                                            aaGCCGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcg         <  2:486111‑M1/143‑124 (MQ=255)                                                                                                                             aGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgt        <  2:575010‑M1/143‑125 (MQ=255)                                                                                                                             aGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgt        >  1:180525‑M1/1‑19 (MQ=255)                                                                                                                                cgacgacgGCCTGCATctcctcctccttctttgaagatgcggaagatcccgccggtacctcccctgtgccatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatg     <  2:205009‑M1/143‑128 (MQ=255)                                                                                                                                   cgacgGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgt  >  2:172010‑M1/1‑13 (MQ=255)                                                                                                                                               |                                                                                                                                   CAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTA  >  NC_000913/4001501‑4001773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑