Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,533,261 A→C 13.5% K70N (AAA→AAC pptA → 4‑oxalocrotonate tautomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,533,2610AC13.5% 244.3 / 3.4 101K70N (AAA→AACpptA4‑oxalocrotonate tautomerase
Reads supporting (aligned to +/- strand):  ref base A (58/25);  new base C (2/15);  total (60/41)
Fisher's exact test for biased strand distribution p-value = 1.13e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTCCTCGACCAGAGGCAAACCAAATACCGGG  >  NC_000913/1532987‑1533525
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                        
tACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATAATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGaaa                                                                                                                                                                                                                                                                          >  2:829093/1‑275 (MQ=255)
  ccGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGGATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAAcc                                                                                                                                                                                                                                                                        <  2:386410/275‑1 (MQ=255)
  ccGGACAAATGACATTCAAAATGTGTGTTAATTTTACTGACAACTTTCAAAAGGAGCGTAAGCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAGAAAGCAGCACTTGCTGCAGATATTACCGACGTTATGATTCGTCATCTGAAAAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAAcc                                                                                                                                                                                                                                                                        <  2:640230/275‑1 (MQ=255)
    ggACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAAcc                                                                                                                                                                                                                                                                        <  2:182665/273‑1 (MQ=255)
      acacATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTg                                                                                                                                                                                                                                                                      >  1:354960/1‑273 (MQ=255)
      aaacaTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGt                                                                                                                                                                                                                                                                    <  1:246948/273‑1 (MQ=255)
          aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCCGATTCAACCAGAATCTTGGCAAGCTATCTGGGTTGCCGAAATCGCGCCCCACATGGAGGCTTTGATAAAGAAACCTGGGtata                                                                                                                                                                                                                                                                >  1:236731/1‑275 (MQ=255)
          aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAACCCTGGTtat                                                                                                                                                                                                                                                                 >  1:823213/1‑274 (MQ=255)
          aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCCCGCCCCAAATGGAGGCTTTGATAAAGCAACCTGATtat                                                                                                                                                                                                                                                                 >  2:254482/1‑274 (MQ=255)
          aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGAAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTtata                                                                                                                                                                                                                                                                >  1:694170/1‑275 (MQ=255)
              cagtCACAATTGGTGTTAATTTTACTGACATCGTTCAAAAGGGGCGGAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCAt                                                                                                                                                                                                                                                            <  1:629394/272‑1 (MQ=255)
                        gtgtgtTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGAATGAATGCTTa                                                                                                                                                                                                                                                   >  2:765601/1‑274 (MQ=255)
                           tgtTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAAccg                                                                                                                                                                                                                                               >  1:719161/1‑274 (MQ=255)
                           tgtTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAAccc                                                                                                                                                                                                                                               >  1:127253/1‑275 (MQ=255)
                           tgtTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAAccc                                                                                                                                                                                                                                               >  2:540665/1‑275 (MQ=255)
                           tgtTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAAccc                                                                                                                                                                                                                                               >  2:258543/1‑275 (MQ=255)
                           tgtTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAAccc                                                                                                                                                                                                                                               >  1:177007/1‑275 (MQ=255)
                           tgtTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATAGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAAcca                                                                                                                                                                                                                                               >  2:350770/1‑274 (MQ=255)
                                 ttttACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACccgcc                                                                                                                                                                                                                                            >  2:300469/1‑272 (MQ=255)
                                   ttACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAac                                                                                                                                                                                                                                       >  2:720330/1‑275 (MQ=255)
                                   ttACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGCCTTTGATAAAGAAACCTGGTTATAGCATGAATTCTAAACccgcctaaac                                                                                                                                                                                                                                       >  1:319965/1‑270 (MQ=255)
                                     aCTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATCGAGGCTTTGATAAAGAACCCTGGTTATAGCACGAATGCTTAACCTCCCGTAacac                                                                                                                                                                                                                                     >  1:376003/1‑275 (MQ=255)
                                     aCTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCCCTACAGCAGATTCAACCAGAATATTGGCAAGCTATCTGGGATGCCGAAATCGCCCCACAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAAGACTTCACCCGCCGTCacac                                                                                                                                                                                                                                     >  1:413805/1‑275 (MQ=255)
                                     aCTGACATATTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATATTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTAACGAAGTTATTATTCATAATCAGAACAGCAAAGACAGTTAGATAAGCATTTCTCTACAGCAGATTCAACCAGAAACTTGGCAAGTTATCTGGGATGCCGAAATCGCGCAACAAATGGAGGACTTGATAAAGAAACCTAGTTATAGCATGAATGATTAACCCCCCGtcaacc                                                                                                                                                                                                                                     >  1:376204/1‑270 (MQ=255)
                                           aTCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAg                                                                                                                                                                                                                                                               >  1:441942/1‑243 (MQ=255)
                                           aTCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGc                                                                                                                                                                                                                               >  2:618549/1‑275 (MQ=255)
                                           aTCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACATGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGc                                                                                                                                                                                                                               >  2:327600/1‑275 (MQ=255)
                                               ttCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATg                                                                                                                                                                                                                                                           <  2:441943/243‑1 (MQ=255)
                                                       gAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGTATGCCGAAATCGCGCCCCAAATGGAGACTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTACCCCGCCGTAACACGCTTGCCGCTGGTACCa                                                                                                                                                                                                                    >  2:320664/1‑274 (MQ=255)
                                                       gAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTACACCAGAATCTTGGCAAGCTATATGGGATGCCGAAATAGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGCATGCTTAACCCGCCGTAACACGCTTGCCGCAGGTAACaa                                                                                                                                                                                                                   >  2:413649/1‑275 (MQ=255)
                                                          cGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTACAACGCTTGCCGCTGGTAACAACCg                                                                                                                                                                                                                >  2:34506/1‑275 (MQ=255)
                                                          cGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACg                                                                                                                                                                                                                >  2:225161/1‑275 (MQ=255)
                                                              aaagtgcccaacatcaaactaaaaTTTTTTCCGCGTGAAATGGACGAAAAAAAAAAAACAGCACTGGCTGCAAATATTACCGCAGTTATTATTCGTCATCTAAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAGTCTTGGAAAGCTATCTGGGATGCCAAAATCGCGCCCCAAATGGGGGCTTTGAAAAAAAACCCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTccc                                                                                                                                                                                                            <  1:320479/254‑1 (MQ=255)
                                                                  tGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTACCAAACGTCCCAGc                                                                                                                                                                                                         >  2:71364/1‑274 (MQ=255)
                                                                    ccGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATCAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACCCTTGACGCTGGTAACAACCGTCCCAGCAcc                                                                                                                                                                                                      >  2:408153/1‑275 (MQ=255)
                                                                      gCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAAcc                                                                                                                                                                                                                                                                        <  2:431025/207‑1 (MQ=255)
                                                                       cacaTCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACATGGTTATAGCATGAATGCTTAACCCGCCGTAACAAGCTTGCCGCTGGAAACAAACGTCCCAGCACCTaa                                                                                                                                                                                                   >  1:427011/1‑275 (MQ=255)
                                                                       cacaTCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCGTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCCGACCCTaa                                                                                                                                                                                                   >  1:54184/1‑275 (MQ=255)
                                                                        acaTCGACATTAAATTGTTTCCGCGTGACCTGTACAAAAAACAAAAAGCAGCACTTGCTGCAGATATTACCGCCTGTATTATTGGTCATCGGAAAAGTAAATACAGTGCGATAAGAATTCCTCTACAGCAGATTCAACCAGAATTTTGGCAAGCTATCTGGGAGGCCAAAATCGCGCCCCAAAGGGAGGCTTTGATAAAGAACCCTGGTTATAGAATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTaaa                                                                                                                                                                                                  <  1:71364/275‑1 (MQ=255)
                                                                        aattcgaatatAAAGGTTTCCCGCGTGACCTGGAAGAAAAACAAAAAGCAGCATTGTCTGCAGATTGCCCCGACGTTAATATTCGACGTCTAAACAGTAAGAACTGTTGGATTACCATGTCTCTACAGCAGCTTCAACCAGAGTATGGGCACGATATCTGGGGTGCCAAATTCGCCCCCAAAATTGAGCTTTGATAAAGAACCCTTGTTATAGCATGAATGCTTATCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTaaa                                                                                                                                                                                                  <  1:505142/264‑1 (MQ=255)
                                                                            aaaaatgaaaTGTTTTCCGCGGAAAAGGAACAAAAACAAAAAAGCAGACCTTGCTGCAGATATGACCGCCGTTTTTATTGGACATCTGAACAGAAAAGACAGTTCAATAAGCATTGCTCTAAAGCAGATTCACCCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGGGGCTTTGATAAAGAACCCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAatgat                                                                                                                                                                                              <  1:804272/268‑1 (MQ=255)
                                                                               cATTAAATGTTTTCCGCGTGACCTGTACGAACAAAAAAAAGCAGCACTTGCTGAAGATATTACCAACGTTATGATTCGTCATCTAAACAGTAAAGACATTTCGATAAGCATTGCCCTACAGCAGATTCACCCAGAATCTGGGAAAGATATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGAAAAAGAACCCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCa                                                                                                                                                                                           <  2:766898/275‑1 (MQ=255)
                                                                                          ttCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATACGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCAAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCAGATGATAACAAACGTCCAAGCACCTAAATGATGCAACGTATTCAGc                                                                                                                                                                                >  2:426068/1‑275 (MQ=255)
                                                                                              gcgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAAAAAACGTCCCAGCACCTAAATGATCCAACGTATTGGGCTTa                                                                                                                                                                             >  1:436786/1‑274 (MQ=255)
                                                                                                                 aaaaaaaacaccatttggtcacattatTCCCTACGTTTTTATTCGTCATCTTAACAGTAAGTACATTTAGAAAAGCATTGCCCTACACCAGATTCAACCAGAATCTTGGCAAGATATCTGGGATGCCGAAATCGCGCCCCAAAGGGAGGCTTTGATAAAAAAACCTGGTTATAGCATGAAGTCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAg                                                                                                                                                           <  1:683514/250‑1 (MQ=255)
                                                                                                                 aaaaaaaGCAGCACTTGCTGAAGATTTTACCGACGTTATTATTCGGCAGTTGAACAGTAAAGAAGGTTGGAACAGTGTTGCTCTACAGCAAATTCACCCAGAATCTTGGCAAGCTACCTGGGATGCAGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATTCTTACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAg                                                                                                                                                           <  1:426067/272‑1 (MQ=255)
                                                                                                                   aaaaacgatctcttgagacAGATATTACCGACGTTCTTATTGGTAGTCGGAACAGTAAAGCCAGTTGGATAAGCGTTCCTCAGCAGGAGATTCAACCAGGTTGTTGGAAGGCTATGTGGGTGGCAGAAATGGCGCCCCGAGTGAGGGCTTGGATAAAGAAGCCTGTTTAGAGAATGAATGTTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCg                                                                                                                                                         <  1:34506/256‑1 (MQ=255)
                                                                                                                        gagacagttGCTCCAGATATTCCCGACTTTATTATCCGTCATCTAAAAAGTAAAGAAAGTTGGATAAGATTTGCTCTCAAGCAGATTCAACAAGAATCTTGGAAAACTATATGGGATGCCGAAATCGCGCCCCAAAGGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCtt                                                                                                                                                    <  1:225161/267‑1 (MQ=255)
                                                                                                                         cagccCTTGCGGCAGATATTGACGACGTTATTATTCGTCATCTAAAAAGGAAGGACAGTTCAATAACCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGGTATCTGGGATGCCGAAATCGCGCCCCAAATGGGGGCTTTGATAAAGAAACCGGGTTATAGAATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCg                                                                                                                                                  <  1:323202/275‑1 (MQ=255)
                                                                                                                         cagcaCTTGCTGCAGATAATCCCGCGGGTATTATTCTACAGATAAACAGAAAAGACAGTTGGATAAGCATGGCTCTACAGCAGATGCAACCAGAATCTTGGCAAGCTATGTGGGAGGCCGAAATCGCGCCCAAAATGGAGGCTTTGATAAAGAAACCGGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCg                                                                                                                                                  <  1:320664/275‑1 (MQ=255)
                                                                                                                            caCTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAACCGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTCACATCATCAAACTGCCAGCGGCCTTCgcc                                                                                                                                                >  1:216740/1‑272 (MQ=255)
                                                                                                                                               ctactgtattatTCTTATTCTGAACATTAAAGCCAGTTCGATAACCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCTAAATCGCGCCCCAAATGGAGTCTTTGATAAAGAACCCTGTTTATAGCATGAATGCTTACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCg                                                                                                                            <  2:719162/269‑1 (MQ=255)
                                                                                                                                               cGACGTTATTATTCGACATATAAAAAGTAAAGACATGTCGATAAGATTTCCTCTCCAGCAGTTTCAACCAAAATCTTGGAAAGTTTTCTGGAAGGCCGAAATGGCGCCCAAAATGGAGGCTTTAATAAAGAAACGTGTTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCg                                                                                                                            <  1:720329/275‑1 (MQ=255)
                                                                                                                                                 aCGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAAAAAACGTCCCAGCAACTAAATGATGCAACGTATTGAGATTATAATCATGAAACTGCGAGCGGCCTTCGAAAAATACCTGTTCGTCTGACgct                                                                                                                          >  2:293044/1‑275 (MQ=255)
                                                                                                                                                   tttatttTTCGTACTCTAAACAGTAAAGACAGTTCGACACGAATTGCTCTACAGCAAATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAAGCGCCCCACAAAGGGGGGTTTTGAAAAAGAAACCGGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCgctg                                                                                                                         <  1:413648/273‑1 (MQ=255)
                                                                                                                                                      attattCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGAATCATGTCTTCACCCTCCGTACCACGCTTGCCGCTGGTAACAAACGTCCCAGCACATAAATGATACAACGTATTGCGCTTATCATCATACAACTGCCACCGGCCTTCGCCCATACCCGCGCGGCTGCCgctgctta                                                                                                                     >  1:568371/1‑272 (MQ=255)
                                                                                                                                                            cGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAtaccac                                                                                                               >  2:155068/1‑275 (MQ=255)
                                                                                                                                                             gTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGCTaatact                                                                                                              >  2:585611/1‑275 (MQ=255)
                                                                                                                                                                     aaaaGTAAAAACAGTTCGATAAGAATTGCTCTACAACAGATTCAACCAGAATTTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCaaa                                                                                                      <  2:448101/272‑1 (MQ=255)
                                                                                                                                                                      aCAGTAAGGAATGTTGGATAACCTTTGTTTTACAGCAAATTCACCCATAATTTTGCCAAGCTATGTGGGATGCCAAAACCGCGCCCCAAATGGAGCCTTTAATAAAGAACCCTGTTTATAGCATGAATGCTTACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAAc                                                                                                     <  1:315010/275‑1 (MQ=255)
                                                                                                                                                                          tAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCAAGCGGCCTTCGACAAATACCCGTGCGTCTTCCGCTGCTGATACTACTTCGCCAAACAGCg                                                                                                 >  2:198676/1‑275 (MQ=255)
                                                                                                                                                                                cAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAAcc                                                                                                                                                                                                                                                                        <  1:756877/102‑1 (MQ=255)
                                                                                                                                                                                  gTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTACCACGCTTGCCGCTGGTAACAACCGTCACCGCCCCTAACTGATGCAACGTATTGAGCTTATCATCCTCAAACTGCCAGAGACCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGACACTACTTCCCCaacactcggaccgtac                                                                                         >  1:165910/1‑261 (MQ=255)
                                                                                                                                                                                  gTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTTCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCCTAt                                                                                         >  2:196488/1‑275 (MQ=255)
                                                                                                                                                                                  gTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTAt                                                                                         >  2:107382/1‑275 (MQ=255)
                                                                                                                                                                                  gTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGGAt                                                                                         >  2:194773/1‑275 (MQ=255)
                                                                                                                                                                                  gTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATAAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCGGCTGATACTCCTTCCCCACACCGCGTGTCGttt                                                                                         >  1:836175/1‑273 (MQ=255)
                                                                                                                                                                                    tCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTCACAAGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCCGCTACTACGTCGCCAAACCGCGTGGAGTAttc                                                                                       >  2:712468/1‑275 (MQ=255)
                                                                                                                                                                                    tCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTTCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTAttc                                                                                       >  2:333690/1‑275 (MQ=255)
                                                                                                                                                                                            cATTACTCTACAGAAGATTCAACCAGAATCTTGCAAAGCTTCGTGGGATGCCAAAATCCCCCCCAAAATGGGGGCTTTGATAAAGAACCCTGTTTTGAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTgcg                                                                               <  1:408152/275‑1 (MQ=255)
                                                                                                                                                                                              tggCTCTGCAGCTGTTTCAACAAAAATCTGGGAAAGTTATCTGGGGGGGCAAATTGGGGCCAAAAAGGGGAGCTTTGTGAAAAGTCCGTGGTAATAACGTGATGCCTTAGCCCGCCGTAACACACTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCATATCATCATCAAACTGCCGGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTgcgc                                                                              <  1:721621/272‑1 (MQ=255)
                                                                                                                                                                                                      cagcagATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAACTCGCGCCCCAAATGGAGACTTTGATAAAGAAACCTGTTTATAGCATGAATGCTTAAACCGCCGTAACACGCTTGCCGCTGGTAACAATCGTACCATCACCTAAATGATGCAACGTATTGAGCTTATCATCATAAAACTGCCAGCGGACTTCGACAAATACCACTGCGTTTGCCGCTGCTCATACTACTTCGCCACACCGCGTGTCGTATACTTCTTGCGCaaatggcgc                                                                     >  2:793960/1‑267 (MQ=255)
                                                                                                                                                                                                      cagcagATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCgg                                                                                                                                                        >  1:153031/1‑192 (MQ=255)
                                                                                                                                                                                                      cagcagATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGc                                                                     >  1:371512/1‑275 (MQ=255)
                                                                                                                                                                                                      cagcagATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGATGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGc                                                                     >  2:576147/1‑275 (MQ=255)
                                                                                                                                                                                                         cagATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCAGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTTCGTCCGCCGCTGCTGATCCTACTTAGCCAACCCGCGTCTCGTATTCTTCTTGCACAGAAGTcccca                                                                   >  1:698215/1‑270 (MQ=255)
                                                                                                                                                                                                          agATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAACCCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCtt                                                                                                                                                    <  2:153031/192‑1 (MQ=255)
                                                                                                                                                                                                           gATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGGCGTATTCTTCTTGCGCAGAAGTCGCAGGTa                                                                >  2:589450/1‑275 (MQ=255)
                                                                                                                                                                                                            aTTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTa                                                                >  2:161949/1‑274 (MQ=255)
                                                                                                                                                                                                             ttCAACCAGAATCTGGACATGCTATCTGGTATGCCAAAAGCGCGCCCCAAAGGGAGCCTTGCTAAAAGAAACCTGTTTAAAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTTGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGc                                                              <  2:236731/275‑1 (MQ=255)
                                                                                                                                                                                                                 accaagaagtgtggaaacttatgggggAGGCCAAAAGCGCCCCCAAAATGGGGGTTTTAATAAGAAAACCGGTGTAGAGCATGACGGCACAACCCGCCGGAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAAt                                                           <  1:829092/251‑1 (MQ=255)
                                                                                                                                                                                                                 acagaaaTCTTGGCAAGGATCGTGGGATTCGGAAATCCGGCCCCAATGGGAGGCTTTGAAAAAGAAACCTGGTTATAGCTTGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATc                                                          <  2:436787/270‑1 (MQ=255)
                                                                                                                                                                                                                 aCCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACCACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAAt                                                           >  1:422518/1‑274 (MQ=255)
                                                                                                                                                                                                                      aaTCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATAATCAAACTGCCAGCGGCCTTCGCCAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTAGTATTCTTCTTGCGCCGAAGTCGCAGGTAGCAATCGacc                                                      >  2:402391/1‑273 (MQ=255)
                                                                                                                                                                                                                        atttgggaaagataccggggATGCCGAAATCCGCCCCCAAAGGTAGGTTTTGAAAAAGAACCCGGTTTATAGCATGAAGGCTAAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACt                                                    <  1:107382/258‑1 (MQ=255)
                                                                                                                                                                                                                                           gctcggagatgtgtaaaagagacggcggCTTTGATAAATAAACCTGGTTATAGCATGAATGCTAAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACAtt                                  <  2:273949/248‑1 (MQ=255)
                                                                                                                                                                                                                                             tGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGCAGTCGCAGGTCGCAATCCACACTCACTCCACCCCAGACAtttt                                >  1:205681/1‑274 (MQ=255)
                                                                                                                                                                                                                                               ccGAACTCGGCCCCCAAAAGGAGGCTTTGAAAAAGAAACCTGGTTATAGAAAGAATGCTTACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTcc                             <  1:300469/275‑1 (MQ=255)
                                                                                                                                                                                                                                                cGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGCCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGCCATTTTTcc                             >  1:67733/1‑274 (MQ=255)
                                                                                                                                                                                                                                                  aacacgcgCCCCAAAGGAGGGCTTTTATAATGAACCCTGTTTATGACATAAATGCTAACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTCCt                            <  1:198676/269‑1 (MQ=255)
                                                                                                                                                                                                                                                     tcggcccccAAATGGAGGCTTTGATAAAGAAACCGGGTTAAAGCATGAATGGTAAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTCCTCGAcc                       <  2:376205/270‑1 (MQ=255)
                                                                                                                                                                                                                                                           tccaagtgacggttttAATAAAAAAACCTGGATATAGCATGAATGCTCACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCGTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTCCTCGACCAGAGGc                 <  2:468667/262‑1 (MQ=255)
                                                                                                                                                                                                                                                           cccAAATGGAGGCTTTTATAAAAAACCCCGTTTATAGCATTATTGCTAAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCATCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTCCTCGACCAGAGGc                 <  2:694171/275‑1 (MQ=255)
                                                                                                                                                                                                                                                           cccAAACGAGGGTTTTAAAAAAGAACCCGGGTTAAGGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTCCTCGACCAGAGGc                 <  2:823214/275‑1 (MQ=255)
                                                                                                                                                                                                                                                             cAAAGGGAGGCTTTAATAAAGAACCCGGGTTAAAGCATAAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTCCTCGACCAGAGGc                 <  2:127253/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                     ggCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGCCATTTTTCCACGACCAGAGGCAAACCaaaaa       >  1:391561/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                     ggCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTCCTCGACCAGAGGCAAACCAAATa       >  2:217366/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                         ttGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATAATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGATGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGAAATCGACACTCCACCCACGCCAGACCTTTTTCCTCGACCCGAGGAACACCAAATACCgg   >  2:760318/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                          tGATAAAGAAACCTGGTTAGAGAAAGAATGCTACACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTGCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTCCTCGACCAGAGGCAAACCAAATACCggg  <  1:765600/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                               aaGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACCCTCCATCCACGCCAGa                                      >  1:273949/1‑234 (MQ=255)
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                        
TACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAGCGTGTCGTATTCTTCTTGCGCAGAAGTCGCAGGTAGCAATCGACACTCCATCCACGCCAGACATTTTTCCTCGACCAGAGGCAAACCAAATACCGGG  >  NC_000913/1532987‑1533525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: