Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,708,751	A→G	9.6%	E611E (GAA→GAG)	rsxC →	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,708,751	0	A	G	9.6%	87.9 / 2.7	48	E611E (GAA→GAG)	rsxC	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (19/23);  new base G (3/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

CAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAA  >  NC_000913/1708481‑1709024                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                  aaaggcaatttgtcaagacggggAAGCCCGTAAAGGGAAAGCCAGAGCGAAACAGGCACACATGCAGCAAACGAACGCGGGAGCAACCGTGGCTTATCCACGGAGAACTGCCGTGGAAGCAGCGATCGCCCGCGCCAAAGGGGGCAAGCTGGAACAGAAACAGGCGAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCa                                                                                                                                                                                                                                                                                <  1:616781/254‑1 (MQ=18)  aGTGCAAGTATTGCAGGACGGGAAGCGCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCag                                                                                                                                                                                                                                                                               <  1:310355/274‑1 (MQ=255)   gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGAGCAAACGGGAACAGCAAACGGCTAATGCGGAGACagaa                                                                                                                                                                                                                                                                             >  2:584372/1‑275 (MQ=255)   gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCACGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCACAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAACGCGCGCCAGCTGGAACAGCAACAGGCTAATGCGGAACCcga                                                                                                                                                                                                                                                                              >  2:740037/1‑274 (MQ=255)   gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCAGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACATCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGATATTGACAGTGCCAACGCCAGCAAGCTGGAACACCAACAGGCTAATCAGGAAcgcga                                                                                                                                                                                                                                                                              >  2:323480/1‑270 (MQ=25)     gCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGACCAGCAACAGACTAATGCGGAACCagaaaa                                                                                                                                                                                                                                                                           >  1:259049/1‑273 (MQ=255)                   acggcacgaccGTAAAGCGCAAGCAAGGGCGAAACAGTCAGAACTGCACCAAACTAACGACGCAGCAACCGTGGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCCCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAt                                                                                                                                                                                                                                                                 <  2:713006/262‑1 (MQ=21)                   acaggAAGCCCGTAAAGCGAAAGCCAGAGCAAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAt                                                                                                                                                                                                                                                                 <  2:663675/268‑1 (MQ=255)                   aCGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGCACACCAACAGGCCAATGCGGGACCAGAACAAACGGACGATGCGCGCAAAGCCGGCGCCGAAGCCGCTCTTGCCCGCGACAAAGCCCGCCAACTCGAACAGCAAACGGCTACTGCGGAAcacaaaaaacccagcgaaccg                                                                                                                                                                                                                                                              >  1:591637/1‑254 (MQ=255)                      ggaggccgggaagaggcAAGACAGGGCGAAAGAGGCAGAACTGAAGCAAAATAACGGGGGAGCAACCGGGGCGGAACAAGGAAAAACTGCCGGTCAAGCAGCTATCACCGGGGCCGAAGCGGCGAAGCGGGAACAGCAACAGGCTAATGCGGAACCAGCACACGAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCgcgc                                                                                                                                                                                                                                                           <  1:416673/259‑1 (MQ=17)                                     cgaaGCCAGAGCGAAACAGGCATACTTGCAGCAACGTAACGACCGAGCAACCCTTGCTGACCCCCGTAAAACGCCCGTTGAAGCAGCTATCGCCGGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGCCTACTGCCGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                                           <  1:210409/273‑1 (MQ=14)                                      cAAGCCCGCGCGAAACATGCAGAACTGCAACAACCTAAAGCCGCAGCAACCGTTGCTGATCCACGGAAAACTGCCGTTGAAGCAGCTATCGCCCGCGACAAAGCGCGCAAGCTGGAACAGCAAAAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                                           <  2:836018/274‑1 (MQ=18)                                      cAAGACAGAGCGAAACAGACAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGGAAAACTGCCGTTGAAGCAGCTACCGCCCGCGCCAAAGCGCGCAAGGTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                                           <  2:785112/274‑1 (MQ=255)                                              agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAACGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAAAGGTCGACCCCCGCAACGCCGCCGCCGAAGCCGccgtt                                                                                                                                                                                                                                   >  2:40987/1‑270 (MQ=255)                                              agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAtt                                                                                                                                                                                                                                   >  2:481560/1‑274 (MQ=255)                                              agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGCACAGCAACAGGCTAATGAGGAGCCAGAAAAAAAGGTCAAACCGCGAAAAgccgcagccgcaacggccattg                                                                                                                                                                                                                                  >  1:318868/1‑258 (MQ=18)                                               gCGAAACAGCCAGAACGTCACCAAACTACCGACGCCGCAACCGTTGCTGGTCCACGTAAAACTGCCTTTGAAGCAGCTATAGCCCGCGCCAAAGCGAGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc                                                                                                                                                                                                                                 <  2:850050/275‑1 (MQ=18)                                                  caggaGGCAAAACCTCAGGAAACTAAAGGCGCAGCACGCGTGGCTGAGCGAGATAAAACTGCCGCTGGAGCAGCCACTGCCGCGGACAAAGGGGGCAAGCAGGAACAGCAACAGGCAAATGCGGAACCAGAACACCAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGcc                                                                                                                                                                                                                                <  1:574903/269‑1 (MQ=14)                                                   aaCAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACCGGCTACTGCGGAACCAGACCAACAGGTCGCTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACCGGCCACTGCGGAACCAGAACAACAGGTGCAACACGGCAAAGCCGCCGCCCCAGCCGccctttccagc                                                                                                                                                                                                                             >  1:591998/1‑265 (MQ=18)                                                   aaCAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCGGACCAACAGGTCGATCCGCGCAAAGCCGCCGTc                                                                                                                                                                                                                                               >  2:210409/1‑257 (MQ=255)                                                         cAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACTGGAAAAGCAACAGGCTAATGCCGAACAAGAAACACAGGTCGACCCGCCCCAAGCCGCCGGTGAAGGCGCCATTGCCCggcccaca                                                                                                                                                                                                                       >  1:868523/1‑268 (MQ=18)                                                                 cagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGCTCCGCGCAAAGCCGACGTCGAAGCCGCTATTGACCGCGCCAAAGAACGCa                                                                                                                                                                                                                >  2:528981/1‑274 (MQ=255)                                                                 cagcaAAATAACGACGGGGCCACCGGTGCGAGACCACGTAAAACTTCCGTGGAAGCAGCTATCGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGGTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa                                                                                                                                                                                                               <  2:492226/275‑1 (MQ=17)                                                                  agcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCTGGCCAAAGACCGCAACCTGGAACCGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAACCGCGCAAAGCCGCCGTCGAAGCCGCGAATGCCCggtcacaaagaaccaaa                                                                                                                                                                                                              >  1:130464/1‑259 (MQ=18)                                                                       aCTAACGATGCAGCAACCGTTGCTGATCCAGCGAAAACTGCAGTTGAAGCAGCTATCGCCCGGCCCAAAGCGCGCAAGCTGGAAAAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGc                                                                                                                                                                                                             <  2:579504/271‑1 (MQ=255)                                                                                               aaaccACGAAAAACGCGCGCTGAGGAAGCCATAGCACGGGCGAAAACGCGGAAGGTGGAAGAGCAACAGGCGAATAAGGAAGCAGAACCACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc                                                                                                                                                                                  <  1:657644/271‑1 (MQ=14)                                                                                                   acgtaaaaaCTGCCGTCGAAGCAGCTACCGCCCGCGCCAAACGGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAAAAGGTCGATCCGCGCAAAGCCGCCGGCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                                                                                                                                                                <  2:259049/267‑1 (MQ=255)                                                                                                               ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGACGAAGCGGCTATTGCCCGTGCCAAAGCGCGAAAGACGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAACGCCGCGGTCGCAGCCGCCATTGACCGCGCCAAACCACGCAAGCGCGCACAGCGACAGGATGACGCGGAGCACGaaaaacctggcca                                                                                                                                                                  >  1:196293/1‑267 (MQ=14)                                                                                                                                     cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGTGCCAAAGCAAGAAAACCCGAACCGCAACCGGCTAATGACGAACCCCAAACACAGGTCAATCCgcgccacgcgcccccataa                                                                                                                                            >  1:811874/1‑259 (MQ=14)                                                                                                                                                              cAAAAGGCAAATGCGTAACCAGGACAACAGGCCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAgcgcg                                                                                                                  <  2:189620/275‑1 (MQ=255)                                                                                                                                                                              aaCCAGAACAACAGGCCGATCCGCGCAAAGCCCCCCGCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCaa                                                                                                  <  2:462611/275‑1 (MQ=255)                                                                                                                                                                                ccAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCCAAGCCGCCATTGCCCGTGCCAAAGCCCGCAAGCTGGAACCACAACCAGCAAATGCCGAGCCAGAACAACCGGTCCCTCCGCGCCAACCCGCCGACGCAACCGCCAATGCCCTAGCCCAACCCCGACAACCGGAACCACAAc                                                                                                 >  1:871836/1‑274 (MQ=14)                                                                                                                                                                                      caacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTa                                                                                                                                                                                           >  1:643819/1‑178 (MQ=255)                                                                                                                                                                                          aGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc                                                                                                                                                                                       <  2:643820/178‑1 (MQ=255)                                                                                                                                                                                             tCGGTCCGCGCAAAGCCCCGCTCAAAGCCGCAATTGCCCGGTCCAAAGCGCGGCAGCTAGAACAGAAAGAGGCTAATGCGGAACCAGAAGAACAGGTCTATCCGCGCAAAGCCGCCGTGGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGa                                                                                    <  2:871837/274‑1 (MQ=17)                                                                                                                                                                                               gATCCGCGCAAAGCCGCCGTCGAAGCCGCTCAGGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGTCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACCCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGcc                                                                                 <  2:808218/275‑1 (MQ=255)                                                                                                                                                                                                    gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCCATTGCCCGAGGCAAAGCCCGCAACCGGGAACACCACCAGGCTAATGCGGCGCCacaa                                                                             >  2:272855/1‑274 (MQ=14)                                                                                                                                                                                                                                     tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCCGAACAACAGGTAGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTTCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGGCAGAAGAACAGGTTGAACCGCGCAAAGcctgcgacacaa                                           >  2:118474/1‑264 (MQ=255)                                                                                                                                                                                                                                     tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAAAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAACCGGGAACAGCAACCGGCTAATGCGGAGCGAAAAAAAAAGGCTACACCGCGCaaacacgaagacgaag                                           >  2:188804/1‑262 (MQ=14)                                                                                                                                                                                                                                      gcacaaGCGTGCAAGCTGAAACAGCAACAGGCGAAGGCGGAACCAGAAGAACAGGTCGACCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGGCCGGGCAAAAGCACGAAAGCTGGAACAGCAACAGGCTAATGACGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAg                                           <  2:87528/270‑1 (MQ=14)                                                                                                                                                                                                                                      gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCCAAGCACGCAAACCGGAACAGCAAAAGGCTACTGCCGGGCCCGAACAAAAGGTCGATCCCCCCCAAACCCCCGTCGCACCCGCCCTTTCCCCACCCAAACCCCCCCAACGGGCACAGCCACAGGTCAATGCCGCGCCCGAACAACAggcttacaccgccaaacacgccgctgacc                                           >  1:850008/1‑247 (MQ=2)                                                                                                                                                                                                                                       ccAAAGCGCGACAGCTGGACCAGCAACAGGCTAATGCGGAACCAGAAGCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTAGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGc                                          <  2:754568/274‑1 (MQ=255)                                                                                                                                                                                                                                               cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCAGCTATTGCCCGAGCCAAAGCGCGAAAACGGGAAAAGCAACCGGCTACTACGGCGCCAGAAGAACAAGTTGATCCGCGCAAACCCGCCGACAACCCGGCCATTGc                                 >  2:394395/1‑275 (MQ=14)                                                                                                                                                                                                                                                              cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGATGGAACCGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCTCAAAGCTGCCGTCGAAGAGGCGAtggaccgggcaacagacgg                  >  2:526658/1‑257 (MQ=14)                                                                                                                                                                                                                                                                gaaggaaaaTGAGGAACCAGAAGGACAGAGGGATCCGCGCAAATCCGCCGTCGAAGCCGATAGTGCCCGTGCCAAAGCACGCAAGCTGGAACCGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCa                <  2:405149/268‑1 (MQ=17)                                                                                                                                                                                                                                                                    gCTAATACGGAACCAGAAGAACATGTCGATCCGCGCAAAGCCGCCTTCGAGGCCGCTATGGCCCGTGCCAAAGCACGCAAGCTGGAACCGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCt            <  2:591999/275‑1 (MQ=255)                                                                                                                                                                                                                                                                     cTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                           <  2:707051/211‑1 (MQ=255)                                                                                                                                                                                                                                                                       aaggCGGAACCAGAAAACCAGGTCGCTCCGCGCAAAGCCCCCGTCTAAGCCCCCATTGCCCGTGCCAAAGCACCCAAGCTGGAACAGCAACAGCCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCt            <  2:740879/269‑1 (MQ=17)                                                                                                                                                                                                                                                                              aaCCAGAAGAACAGGGCGAGCCGCGGAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCGAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa  <  2:591638/275‑1 (MQ=255)                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                  CAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAA  >  NC_000913/1708481‑1709024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑